##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933687.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	900790
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50964597741982	34.0	31.0	34.0	31.0	34.0
2	32.735343420775095	34.0	31.0	34.0	31.0	34.0
3	32.74448872656224	34.0	31.0	34.0	31.0	34.0
4	36.208614660464704	37.0	37.0	37.0	35.0	37.0
5	36.27052587173481	37.0	37.0	37.0	35.0	37.0
6	36.27360650096027	37.0	37.0	37.0	35.0	37.0
7	36.282992706402155	37.0	37.0	37.0	35.0	37.0
8	36.234360949832926	37.0	37.0	37.0	35.0	37.0
9	38.08468899521531	39.0	39.0	39.0	37.0	39.0
10	37.71799864563328	39.0	38.0	39.0	35.0	39.0
11	37.5745057116531	39.0	37.0	39.0	35.0	39.0
12	37.60925187890629	39.0	37.0	39.0	35.0	39.0
13	37.63430988354666	39.0	37.0	39.0	35.0	39.0
14	39.020590814729296	40.0	39.0	41.0	35.0	41.0
15	39.058690704825764	41.0	39.0	41.0	35.0	41.0
16	39.121100367455234	41.0	39.0	41.0	35.0	41.0
17	39.084657911388895	41.0	39.0	41.0	35.0	41.0
18	38.52569078253533	39.0	38.0	40.0	35.0	41.0
19	37.933176434019025	38.0	37.0	40.0	35.0	41.0
20	37.20058282174536	38.0	35.0	40.0	34.0	41.0
21	37.13676106528714	38.0	35.0	40.0	34.0	41.0
22	37.120165632389345	38.0	35.0	40.0	34.0	41.0
23	36.9691470820058	38.0	35.0	40.0	34.0	41.0
24	36.87627304921236	37.0	35.0	40.0	34.0	41.0
25	36.82690860244896	37.0	35.0	40.0	33.0	41.0
26	36.81433075411583	37.0	35.0	40.0	33.0	41.0
27	36.7606611973934	37.0	35.0	40.0	33.0	41.0
28	36.675393820979366	37.0	35.0	40.0	33.0	41.0
29	36.47615093418	37.0	35.0	40.0	33.0	41.0
30	36.15612850941951	37.0	35.0	40.0	33.0	41.0
31	35.63753483053764	37.0	35.0	40.0	30.0	41.0
32	34.808869991896	37.0	35.0	40.0	22.0	41.0
33	33.53180430510996	37.0	33.0	40.0	15.0	41.0
34	32.42970836709999	37.0	31.0	40.0	10.0	41.0
35	31.715319885877953	37.0	30.0	40.0	7.0	41.0
36	31.306300025533144	37.0	25.0	40.0	7.0	41.0
37	31.073562095493955	36.0	24.0	40.0	7.0	41.0
38	30.882776229753883	36.0	23.0	40.0	7.0	41.0
39	30.76926142608155	36.0	23.0	40.0	7.0	41.0
40	30.58456799031961	36.0	22.0	40.0	7.0	41.0
41	30.40382886133283	36.0	22.0	40.0	7.0	41.0
42	30.18022846612418	36.0	21.0	40.0	7.0	41.0
43	30.047903506921703	35.0	20.0	40.0	7.0	41.0
44	29.893373594289457	35.0	20.0	40.0	7.0	41.0
45	29.80850919748221	35.0	20.0	40.0	7.0	41.0
46	29.65593312536773	35.0	20.0	40.0	7.0	41.0
47	29.615874954206863	35.0	20.0	40.0	7.0	41.0
48	29.49240333485052	35.0	20.0	40.0	7.0	41.0
49	29.453368709688164	35.0	20.0	40.0	7.0	41.0
50	29.295637162934757	35.0	20.0	40.0	7.0	41.0
51	28.62347717003963	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	2.0
11	3.0
12	8.0
13	8.0
14	14.0
15	28.0
16	52.0
17	148.0
18	318.0
19	682.0
20	1254.0
21	2092.0
22	3368.0
23	5892.0
24	11989.0
25	27129.0
26	47544.0
27	54776.0
28	46515.0
29	35543.0
30	28377.0
31	25051.0
32	24340.0
33	26578.0
34	36544.0
35	48914.0
36	62324.0
37	73038.0
38	138546.0
39	199601.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.38006638617214	3.2576960223803546	37.42881248681712	42.93342510463038
2	47.84977630746345	3.208627982104597	36.76717103875487	12.174424671677084
3	14.306442123025345	3.6007282496475312	69.57170927741205	12.521120349915074
4	12.11025877285494	3.3609387315578547	69.15807235870736	15.370730136879851
5	11.867360872123358	4.066430577604103	68.76075444887265	15.305454101399881
6	13.920447607100433	4.813330520987134	69.76320785088645	11.503014021025988
7	56.61319508431488	1.2452402890795857	39.316932914441765	2.824631712163767
8	58.64241388114877	1.5415357630524318	37.46833335183561	2.347717003963188
9	54.24216521053742	4.114055440224692	38.71523884590193	2.928540503335961
10	22.408552492811864	26.103309317376972	42.65289357119861	8.835244618612553
11	16.378956249514314	17.711786320896103	53.924444099068594	11.984813330520987
12	14.49949488782069	17.536162701628573	54.457975776818124	13.506366633732615
13	15.031361360583487	16.92236814351847	55.881504013144024	12.164766482754027
14	13.919115443111046	18.534508598008415	54.73606500960268	12.810310949277856
15	12.823188534508597	18.144406576449562	55.713540336815456	13.318864552226378
16	14.798787730769657	18.741993139355454	53.75936677805038	12.699852351824509
17	14.930005883724288	17.807369087134624	53.74993061645888	13.512694412682203
18	15.676572786109972	16.703560208261635	54.0383441201612	13.581522885467201
19	15.100633888031616	18.77807258073469	52.71439514204198	13.40689838919171
20	16.01682967173259	19.892094716859646	51.60781092152445	12.483264689883326
21	15.920580823499373	20.079818825697444	51.872689528080905	12.126910822722277
22	15.801574173780791	17.96356531489026	52.02733156451559	14.207528946813353
23	13.50292521009336	20.375559231341377	51.7995315223304	14.321984036234861
24	13.023346173914007	20.064276912487927	52.80431621132562	14.108060702272448
25	13.984724519588362	21.247460562395233	51.17363647465003	13.594178443366378
26	14.462860378112547	20.908868881759343	50.780204043117706	13.848066697010402
27	14.287236758845015	19.76898056150712	51.784655691115574	14.15912698853229
28	13.279898755536806	20.46836665593535	51.50046070671299	14.751273881814853
29	14.605956993305877	19.25099079696711	51.09070926631068	15.052342943416336
30	16.287703016241302	19.140754226845324	50.7108205020038	13.86072225490958
31	16.289923289556945	20.692392233483943	48.49154630935068	14.526138167608432
32	16.097869647753637	21.258450915307677	47.4158238879206	15.227855549018084
33	16.43968072469721	22.316522163878375	45.16413370485907	16.07966340656535
34	16.132172870480357	23.839962699408296	43.360383663228944	16.667480766882402
35	16.960889885544912	26.25817338114322	40.247782502026	16.53315423128587
36	17.992650895325216	27.159715360960934	38.39363225613073	16.454001487583124
37	17.214667125523153	27.730103575750174	37.6092096937133	17.446019605013376
38	16.624185437227325	28.798943149901753	37.688473451081826	16.888397961789096
39	17.382519788185927	27.56469321373461	37.26406820679626	17.788718791283205
40	18.62809311826286	27.354322317077234	37.46689017418044	16.550694390479467
41	17.846889952153113	27.94658022402558	36.09831370241677	18.108216121404546
42	18.652516124734955	27.21910767215444	36.278377868315594	17.849998334795014
43	18.1335272372029	26.31234805004496	37.18236214878051	18.371762563971625
44	18.18803494710199	26.81179853239934	36.694679114999055	18.30548740549962
45	18.66605979196039	26.152710398650076	36.645722088389086	18.535507721000457
46	18.66739195594978	26.420142319519535	36.86985867960345	18.04260704492723
47	17.365756724652805	27.437027498085016	37.382408774520144	17.814807002742036
48	17.34743946979873	26.878184704537126	37.5878950698831	18.186480755781037
49	18.30548740549962	25.468533176434022	37.548041163867275	18.67793825419909
50	17.240977364313547	25.837098546831115	37.573685320662975	19.348238768192363
51	17.288713240599918	25.152699297283494	37.1029873777462	20.45560008437039
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	323.0
1	1032.0
2	1741.0
3	43204.5
4	84668.0
5	56846.0
6	29024.0
7	28362.0
8	27700.0
9	27489.5
10	27279.0
11	26555.0
12	25831.0
13	24636.5
14	23442.0
15	22283.0
16	21124.0
17	19862.5
18	18601.0
19	17318.0
20	16035.0
21	14862.5
22	13690.0
23	13270.0
24	12850.0
25	12479.0
26	12506.0
27	12904.0
28	13656.5
29	14409.0
30	15564.5
31	16720.0
32	17946.5
33	19173.0
34	21050.5
35	22928.0
36	25226.5
37	27525.0
38	29020.0
39	30515.0
40	33166.0
41	35817.0
42	39424.5
43	43032.0
44	45908.5
45	48785.0
46	51939.0
47	55093.0
48	56384.5
49	57676.0
50	55339.0
51	53002.0
52	47537.5
53	42073.0
54	37237.0
55	32401.0
56	28412.5
57	24424.0
58	20767.0
59	17110.0
60	14599.0
61	12088.0
62	9835.5
63	7583.0
64	6169.0
65	4755.0
66	3756.5
67	2758.0
68	2225.5
69	1693.0
70	1398.0
71	1103.0
72	996.5
73	890.0
74	917.0
75	778.5
76	613.0
77	418.0
78	223.0
79	147.5
80	72.0
81	62.5
82	53.0
83	29.0
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	900790.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.67318909499868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68721343457999	36.25910002895674
2	8.592477425201572	8.020766473218806
3	3.8709028908512666	5.420021477792347
4	2.2575235302622785	4.214632904573612
5	1.5684747150951388	3.6602858484179803
6	1.17731987329294	3.296956384290077
7	0.8715037406758189	2.847310121989284
8	0.6839794214305878	2.5538800698814095
9	0.5225161443668078	2.1948745330099486
>10	2.6580504209503855	19.296828913827625
>50	0.06363155979630487	2.019290919518703
>100	0.0430578960326227	3.298392774167431
>500	0.002392105331657384	0.7568562635872058
>1k	7.176315994972151E-4	0.4022648056799974
>5k	0.0	0.0
>10k+	2.3921053316573837E-4	5.758538481088789
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51578	5.725862853717293	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	1452	0.16119184271583833	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1130	0.1254454423339513	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1021	0.11334495276368521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3304099734677336E-4	0.0	0.0	0.1031316955117175	0.0
2	3.3304099734677336E-4	0.0	0.0	0.31738807047147505	0.0
3	3.3304099734677336E-4	0.0	0.0	0.5017817693358052	0.0
4	3.3304099734677336E-4	0.0	0.0	0.7385739184493612	0.0
5	3.3304099734677336E-4	0.0	0.0	1.231585608188368	0.0
6	3.3304099734677336E-4	0.0	0.0	1.9556167364202532	0.0
7	3.3304099734677336E-4	0.0	0.0	2.651894448206574	0.0
8	3.3304099734677336E-4	0.0	0.0	3.709077587451015	0.0
9	3.3304099734677336E-4	0.0	0.0	4.408796722876586	0.0
10	4.4405466312903117E-4	0.0	0.0	5.268042496031262	0.0
11	4.4405466312903117E-4	0.0	0.0	6.452225268930605	0.0
12	4.4405466312903117E-4	0.0	0.0	7.4447984546897725	0.0
13	4.4405466312903117E-4	0.0	0.0	7.845668801829505	0.0
14	4.4405466312903117E-4	0.0	0.0	8.046714550561173	0.0
15	4.4405466312903117E-4	0.0	0.0	8.229332030772989	0.0
16	4.4405466312903117E-4	0.0	0.0	8.56825675240622	0.0
17	4.4405466312903117E-4	0.0	0.0	9.030961711386672	0.0
18	4.4405466312903117E-4	0.0	0.0	9.51376014387371	0.0
19	5.55068328911289E-4	0.0	0.0	9.796178909623775	0.0
20	6.660819946935467E-4	0.0	0.0	10.071270773432209	0.0
21	7.770956604758046E-4	0.0	0.0	10.362570632444854	0.0
22	7.770956604758046E-4	0.0	0.0	10.638994660242675	0.0
23	7.770956604758046E-4	0.0	0.0	10.894992173536563	0.0
24	7.770956604758046E-4	0.0	0.0	11.082161214045449	0.0
25	7.770956604758046E-4	0.0	0.0	11.232584731180408	0.0
26	7.770956604758046E-4	0.0	0.0	11.379455811010335	0.0
27	7.770956604758046E-4	0.0	0.0	11.539759544399915	0.0
28	7.770956604758046E-4	0.0	0.0	11.691737252855827	0.0
29	7.770956604758046E-4	0.0	0.0	11.85315112290323	0.0
30	7.770956604758046E-4	0.0	0.0	12.045093751040753	0.0
31	9.991229920403202E-4	0.0	0.0	12.20173403345952	0.0
32	9.991229920403202E-4	0.0	0.0	12.363813985501615	0.0
33	9.991229920403202E-4	0.0	0.0	12.520121226923035	0.0
34	9.991229920403202E-4	0.0	0.0	12.66887953907126	0.0
35	9.991229920403202E-4	0.0	0.0	12.848832691304299	0.0
36	0.001110136657822578	0.0	0.0	13.003585741404766	0.0
37	0.001110136657822578	0.0	0.0	13.167885966762508	0.0
38	0.001110136657822578	0.0	0.0	13.35483297993983	0.0
39	0.001110136657822578	0.0	0.0	13.629591802750918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	25	3.890131E-5	45.0	33
CCGCTTG	20	7.0324726E-4	45.0	1
GCTAGCG	25	3.890131E-5	45.0	1
TCATCCG	25	3.890131E-5	45.0	1
CGTTTTT	30700	0.0	44.684853	1
GTTTTTT	34560	0.0	40.20182	2
CTCGTTG	85	0.0	39.705883	1
CGCCGGT	105	0.0	38.57143	28
CTTCGCG	95	0.0	37.894737	1
TTGTACG	30	1.1397258E-4	37.500004	1
CATCCGG	30	1.1397258E-4	37.500004	2
TTTCGCG	195	0.0	36.923077	1
CACGCCG	110	0.0	36.81818	26
TATTGCG	55	2.748493E-9	36.81818	1
GCCGATT	165	0.0	36.81818	9
CGGTAGT	110	0.0	36.81818	12
CTTTGCG	190	0.0	36.71053	1
TGGGCGA	1355	0.0	36.531364	6
GTTTGCG	175	0.0	36.0	1
CGGGTCT	25	0.002106867	36.0	6
>>END_MODULE