##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933686.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767928
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.095827994291135	33.0	31.0	34.0	30.0	34.0
2	32.284107364232064	34.0	31.0	34.0	30.0	34.0
3	32.41768108468502	34.0	31.0	34.0	30.0	34.0
4	35.95497754997864	37.0	35.0	37.0	35.0	37.0
5	35.96583924534592	37.0	35.0	37.0	35.0	37.0
6	35.93839917283912	37.0	35.0	37.0	35.0	37.0
7	35.98867471950496	37.0	35.0	37.0	35.0	37.0
8	35.80893911929243	37.0	35.0	37.0	35.0	37.0
9	37.589521413465846	39.0	37.0	39.0	35.0	39.0
10	37.0871357731454	39.0	37.0	39.0	33.0	39.0
11	37.145922534404264	39.0	37.0	39.0	34.0	39.0
12	37.096589784458956	39.0	37.0	39.0	33.0	39.0
13	37.025899563500744	39.0	37.0	39.0	33.0	39.0
14	37.980029377754164	40.0	37.0	41.0	33.0	41.0
15	38.02898709253993	40.0	37.0	41.0	33.0	41.0
16	38.15860731735267	40.0	37.0	41.0	33.0	41.0
17	38.15755904199352	40.0	37.0	41.0	33.0	41.0
18	37.95624068923128	39.0	37.0	41.0	33.0	41.0
19	37.73533846923149	39.0	37.0	41.0	33.0	41.0
20	37.385471033742746	39.0	35.0	40.0	33.0	41.0
21	37.24545660530675	39.0	35.0	40.0	33.0	41.0
22	37.307634309466515	39.0	35.0	40.0	33.0	41.0
23	37.24216332781198	39.0	35.0	40.0	33.0	41.0
24	37.15236975341438	39.0	35.0	40.0	33.0	41.0
25	37.05775671677553	39.0	35.0	40.0	33.0	41.0
26	36.98591925284662	38.0	35.0	40.0	33.0	41.0
27	36.88984904834828	38.0	35.0	40.0	32.0	41.0
28	36.719592201352214	38.0	35.0	40.0	32.0	41.0
29	36.60675219551833	38.0	35.0	40.0	31.0	41.0
30	36.52268181392005	38.0	35.0	40.0	31.0	41.0
31	36.227685408007	38.0	35.0	40.0	30.0	41.0
32	35.91720577970852	38.0	35.0	40.0	30.0	41.0
33	35.534741538269216	38.0	35.0	40.0	27.0	41.0
34	35.17657905428634	38.0	35.0	40.0	24.0	41.0
35	35.00033596899709	38.0	35.0	40.0	23.0	41.0
36	34.970592295111	38.0	35.0	40.0	23.0	41.0
37	34.899334312591805	38.0	35.0	40.0	23.0	41.0
38	34.875515673344374	38.0	35.0	40.0	22.0	41.0
39	34.80383838068152	38.0	34.0	40.0	22.0	41.0
40	34.69960725484681	38.0	34.0	40.0	21.0	41.0
41	34.548720713400215	38.0	34.0	40.0	20.0	41.0
42	34.383744309362335	38.0	34.0	40.0	19.0	41.0
43	34.24848293069142	38.0	34.0	40.0	18.0	41.0
44	34.194070277421844	38.0	34.0	40.0	18.0	41.0
45	34.248032367617796	38.0	34.0	40.0	20.0	41.0
46	34.08870362846517	38.0	33.0	40.0	20.0	41.0
47	33.927810940609014	37.0	33.0	40.0	18.0	41.0
48	33.86258737798335	37.0	33.0	40.0	18.0	41.0
49	33.90163270514944	37.0	33.0	40.0	18.0	41.0
50	33.805692721192614	37.0	33.0	40.0	18.0	41.0
51	32.668316560927586	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	3.0
11	5.0
12	13.0
13	3.0
14	27.0
15	36.0
16	50.0
17	95.0
18	179.0
19	394.0
20	648.0
21	1130.0
22	1881.0
23	3087.0
24	5519.0
25	10412.0
26	16209.0
27	18183.0
28	17650.0
29	16586.0
30	17397.0
31	19678.0
32	23863.0
33	32087.0
34	47270.0
35	56242.0
36	73410.0
37	99306.0
38	163893.0
39	142619.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.61149482764009	4.842771718181913	44.18552260107718	28.36021085310081
2	33.074064235188715	4.722578158369014	45.16569261701618	17.03766498942609
3	19.387755102040817	4.920252940379828	56.50178141700785	19.190210540571513
4	16.359997291412736	5.087846777302039	56.4410986446646	22.11105728662062
5	14.896839287016492	5.717983977664573	56.3309320665479	23.05424466877103
6	18.80319509120647	6.3607525705534895	59.404527507787186	15.431524830452856
7	69.62944442708171	2.3987925951391276	22.457574147576334	5.514188830202831
8	69.22055192674314	2.929571522330219	21.42974341344501	6.420133137481638
9	62.49400985509058	5.93974955985457	24.643716598431105	6.922523986623746
10	28.18753841505974	27.135486660207732	29.46955964621683	15.207415278515693
11	23.45636570095113	18.486628954797844	37.674625746163706	20.382379598087322
12	20.461032805158816	16.896636142971737	41.32470752466377	21.317623527205676
13	21.898146701253243	16.722270837891053	44.27628631850903	17.10329614234668
14	18.43271765061308	19.10960402537738	44.156743861403676	18.30093446260587
15	14.317878759467034	19.08889895927743	46.45435509579023	20.138867185465305
16	16.074814305507807	20.34761071350439	42.573652738277545	21.003922242710253
17	16.470163869529436	20.586825848256606	40.65719702888813	22.28581325332583
18	17.313602316883873	19.063636174224666	42.66975549791126	20.953006010980197
19	16.9991197091394	22.475023700138554	41.29736121094686	19.228495379775186
20	16.874889312539718	25.520100842787347	38.69386192455543	18.91114792011751
21	15.965559271181673	26.34491775270598	39.530268462668374	18.15925451344397
22	15.298048775406029	23.240069381504515	39.01212092800367	22.44976091508579
23	14.23740246481441	24.863007990332427	37.546098071694225	23.35349147315894
24	14.742658165869718	23.91786730005938	38.180141888302025	23.159332645768874
25	15.94706795428738	25.052478878228168	35.12686085153817	23.873592315946286
26	15.017553728995425	25.017840214186748	36.82181141982061	23.14279463699722
27	16.40466293715036	23.43370732672855	38.292782656707395	21.868847079413694
28	14.659707680928422	22.570475357064726	37.65509266493734	25.114724297069518
29	16.452974758050235	20.902350220333158	36.542748799366606	26.101926222250004
30	19.88063985165276	20.82825473221448	35.96170474315301	23.32940067297976
31	18.03294058818014	23.86343511370858	33.53934223000073	24.56428206811055
32	17.361914137783753	22.72439603712848	32.3598045650113	27.553885260076466
33	18.120969674240293	20.687095665218614	33.403522205206734	27.788412455334356
34	16.12221458261712	19.921919763311145	34.085877842714424	29.869987811357312
35	14.727552583054662	20.856382369180444	34.16921898928024	30.24684605848465
36	15.625553437301415	22.497812294902648	33.85721057182444	28.019423695971497
37	15.139830817472472	23.049686949818213	35.39693825462803	26.41354397808128
38	15.467205258826347	22.718015230594535	34.1499463491369	27.66483316144222
39	16.529935098082113	21.874576783240094	33.69664864414372	27.89883947453407
40	18.383494285922637	20.38810930191372	35.03075809190445	26.19763832025919
41	16.122995905866176	22.421372837036806	32.895922534404264	28.55970872269275
42	17.793855673969436	24.683694304674397	32.13686699794772	25.385583023408444
43	17.11462532945797	23.333697950849558	35.90740277734371	23.64427394234876
44	18.49066579158463	21.393672323446992	33.720999885405924	26.39466199956246
45	20.18848121178027	19.11793814003396	33.08487254013397	27.608708108051793
46	20.511949036888875	20.337193070183663	34.026627496327784	25.124230396599685
47	16.55194236959715	23.513662739215135	35.868597055974	24.06579783521372
48	16.80066360387953	23.513923180298153	35.50541196570512	24.180001250117197
49	20.880733610442643	19.882202498150868	35.32792136762822	23.90914252377827
50	18.91596608015335	19.28865726995239	34.365200904251445	27.43017574564282
51	17.43718161077601	18.638466106197455	32.74317383921409	31.181178443812442
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	738.0
1	1446.0
2	2154.0
3	19001.0
4	35848.0
5	22414.0
6	8980.0
7	8724.0
8	8468.0
9	8543.5
10	8619.0
11	8442.5
12	8266.0
13	7876.0
14	7486.0
15	7055.0
16	6624.0
17	6250.5
18	5877.0
19	5604.5
20	5332.0
21	5091.5
22	4851.0
23	4809.5
24	4768.0
25	5031.5
26	5613.0
27	5931.0
28	6701.5
29	7472.0
30	8097.0
31	8722.0
32	9909.5
33	11097.0
34	12206.0
35	13315.0
36	14300.5
37	15286.0
38	16735.0
39	18184.0
40	21391.0
41	24598.0
42	29342.5
43	34087.0
44	40622.0
45	47157.0
46	60374.5
47	73592.0
48	85977.0
49	98362.0
50	96333.0
51	94304.0
52	79446.0
53	64588.0
54	51538.0
55	38488.0
56	32475.0
57	26462.0
58	22815.0
59	19168.0
60	17057.0
61	14946.0
62	13438.5
63	11931.0
64	10458.0
65	8985.0
66	7589.5
67	6194.0
68	5153.0
69	4112.0
70	3420.5
71	2729.0
72	2326.5
73	1924.0
74	1630.0
75	1125.5
76	915.0
77	662.5
78	410.0
79	305.0
80	200.0
81	132.5
82	65.0
83	50.0
84	35.0
85	24.5
86	14.0
87	13.0
88	12.0
89	6.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	767928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.726913114316574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7544530450577	29.630642878456896
2	10.039787381165596	8.177790967178863
3	4.71287719303931	5.758228198780684
4	2.7922274820735375	4.548752242312637
5	1.918231338061696	3.9061820519198966
6	1.4728395462084123	3.599052493785567
7	1.1407612738613337	3.252177969930934
8	0.9148921026940149	2.9808584940314833
9	0.7131386571447104	2.613954251519372
>10	3.3439526193480256	21.659379406897898
>50	0.12078782583771934	3.3699907080579177
>100	0.07157791577216426	5.444949045477601
>500	0.0022368098678767438	0.5895365371063532
>1k	0.0019172656010372087	1.391852817737847
>5k	0.0	0.0
>10k+	3.195442668395348E-4	3.0766519368060257
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23641	3.0785438218166283	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	2905	0.37829067308393494	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	2009	0.2616130678917815	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1803	0.234787636340907	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1476	0.19220551926743132	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	1425	0.1855642716504672	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1077	0.14024752320530048	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	786	0.10235334562615245	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	785	0.10222312508464335	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9066162452729944E-4	0.0	0.0	0.2836203394068194	0.0
2	5.208821660363992E-4	0.0	0.0	0.9202685668448084	0.0
3	5.208821660363992E-4	0.0	0.0	1.439197424758571	0.0
4	5.208821660363992E-4	0.0	0.0	2.2379702263753893	0.0
5	5.208821660363992E-4	0.0	0.0	4.006495400610474	0.0
6	5.208821660363992E-4	0.0	0.0	6.257227240053755	0.0
7	5.208821660363992E-4	0.0	0.0	8.414590951235011	0.0
8	5.208821660363992E-4	0.0	0.0	11.550692252398662	0.0
9	5.208821660363992E-4	0.0	0.0	13.435504370201373	0.0
10	5.208821660363992E-4	0.0	0.0	16.10893208738319	0.0
11	5.208821660363992E-4	0.0	0.0	20.87161817253701	0.0
12	5.208821660363992E-4	0.0	0.0	24.933978185454887	0.0
13	5.208821660363992E-4	0.0	0.0	26.40312633476055	0.0
14	5.208821660363992E-4	0.0	0.0	27.066990655373942	0.0
15	5.208821660363992E-4	0.0	0.0	27.782812972049463	0.0
16	5.208821660363992E-4	0.0	0.0	29.207686137242032	0.0
17	6.51102707545499E-4	0.0	0.0	30.87281620151889	0.0
18	6.51102707545499E-4	0.0	0.0	32.48481628486004	0.0
19	7.813232490545989E-4	0.0	0.0	33.46212144888583	0.0
20	7.813232490545989E-4	0.0	0.0	34.444505214030485	0.0
21	7.813232490545989E-4	0.0	0.0	35.559713931514416	0.0
22	7.813232490545989E-4	0.0	0.0	36.52490858517986	0.0
23	7.813232490545989E-4	0.0	0.0	37.20570157618943	0.0
24	7.813232490545989E-4	0.0	0.0	37.62852767446948	0.0
25	7.813232490545989E-4	0.0	0.0	37.944833369795084	0.0
26	7.813232490545989E-4	0.0	0.0	38.22519819566418	0.0
27	7.813232490545989E-4	0.0	0.0	38.503219051786104	0.0
28	7.813232490545989E-4	0.0	0.0	38.75688866664583	0.0
29	7.813232490545989E-4	0.0	0.0	39.0083445322999	0.0
30	7.813232490545989E-4	0.0	0.0	39.316055671885906	0.0
31	7.813232490545989E-4	0.0	0.0	39.54954110281172	0.0
32	7.813232490545989E-4	0.0	0.0	39.80881020095634	0.0
33	9.115437905636987E-4	0.0	0.0	40.022241668489755	0.0
34	9.115437905636987E-4	0.0	0.0	40.26210790594951	0.0
35	9.115437905636987E-4	0.0	0.0	40.53153420633184	0.0
36	0.0010417643320727984	0.0	0.0	40.748090966861476	0.0
37	0.0010417643320727984	0.0	0.0	40.96920544634393	0.0
38	0.0010417643320727984	0.0	0.0	41.263113208529965	0.0
39	0.0010417643320727984	0.0	0.0	41.81954558239835	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAG	25	3.8895923E-5	45.0	9
TCGCGCG	30	2.1645574E-6	44.999996	1
CGTTTTT	9870	0.0	43.791794	1
AGACACG	105	0.0	42.857143	24
CTACGAA	90	0.0	42.5	11
TACGAAT	90	0.0	42.5	12
GCTACGA	90	0.0	42.5	10
AGCTACG	90	0.0	42.5	9
AGGCACG	65	0.0	41.53846	10
CGTTAGG	60	3.6379788E-12	41.249996	2
CGACCAA	105	0.0	40.714287	29
GGCGATA	50	1.0804797E-9	40.5	8
CGTTCAT	45	1.9268555E-8	40.0	17
ACGGGAT	45	1.9268555E-8	40.0	5
CGGGCTC	80	0.0	39.375004	6
GTTTTTT	11270	0.0	39.210297	2
TTGGGAC	1780	0.0	38.932587	5
ACACGAC	110	0.0	38.863636	26
CACGACC	110	0.0	38.863636	27
AAACACG	110	0.0	38.863636	40
>>END_MODULE