##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497444
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4580676417848	33.0	31.0	34.0	31.0	34.0
2	32.61982655334068	34.0	31.0	34.0	31.0	34.0
3	32.68366087438988	34.0	31.0	34.0	31.0	34.0
4	36.17101422471675	37.0	35.0	37.0	35.0	37.0
5	36.172063589067314	37.0	35.0	37.0	35.0	37.0
6	36.1431397303013	37.0	36.0	37.0	35.0	37.0
7	36.196112527239244	37.0	37.0	37.0	35.0	37.0
8	36.07345349426267	37.0	37.0	37.0	35.0	37.0
9	37.9078046976142	39.0	38.0	39.0	35.0	39.0
10	37.38493177121445	39.0	37.0	39.0	34.0	39.0
11	37.33668513440709	39.0	37.0	39.0	34.0	39.0
12	37.327751465491595	39.0	37.0	39.0	34.0	39.0
13	37.32817764411673	39.0	37.0	39.0	34.0	39.0
14	38.64649488183595	40.0	38.0	41.0	34.0	41.0
15	38.708125939804276	40.0	38.0	41.0	35.0	41.0
16	38.72689187124581	40.0	38.0	41.0	35.0	41.0
17	38.66665795546835	40.0	38.0	41.0	34.0	41.0
18	38.26533639967514	39.0	38.0	41.0	34.0	41.0
19	37.87756209744212	39.0	37.0	41.0	34.0	41.0
20	37.407404652584006	39.0	35.0	41.0	34.0	41.0
21	37.31355690288756	39.0	35.0	41.0	33.0	41.0
22	37.28759820200867	39.0	35.0	41.0	33.0	41.0
23	37.200716060501286	38.0	35.0	41.0	33.0	41.0
24	37.09410707536929	38.0	35.0	41.0	33.0	41.0
25	37.00051865134568	38.0	35.0	41.0	33.0	41.0
26	36.92120319071091	38.0	35.0	40.0	33.0	41.0
27	36.82343339149733	38.0	35.0	40.0	33.0	41.0
28	36.65957374096381	38.0	35.0	40.0	33.0	41.0
29	36.45556685777696	38.0	35.0	40.0	32.0	41.0
30	36.20894412235347	38.0	35.0	40.0	31.0	41.0
31	35.81571795016122	38.0	35.0	40.0	30.0	41.0
32	35.226546103682026	38.0	35.0	40.0	24.0	41.0
33	34.55610681805389	38.0	34.0	40.0	20.0	41.0
34	33.98847508463264	38.0	34.0	40.0	15.0	41.0
35	33.57498733525784	38.0	33.0	40.0	12.0	41.0
36	33.372582642468295	38.0	33.0	40.0	10.0	41.0
37	33.20710873987826	38.0	33.0	40.0	10.0	41.0
38	33.09437846270133	38.0	33.0	40.0	10.0	41.0
39	32.99664283818882	38.0	33.0	40.0	10.0	41.0
40	32.85699294794992	38.0	33.0	40.0	10.0	41.0
41	32.719821728677	37.0	32.0	40.0	10.0	41.0
42	32.580531677937614	37.0	31.0	40.0	10.0	41.0
43	32.43932985421475	37.0	31.0	40.0	10.0	41.0
44	32.22736227595468	37.0	31.0	40.0	8.0	41.0
45	32.22264415693023	37.0	31.0	40.0	9.0	41.0
46	32.095260974099595	36.0	31.0	40.0	8.0	41.0
47	31.993032381534405	36.0	30.0	40.0	8.0	41.0
48	31.89427151598974	36.0	30.0	40.0	8.0	41.0
49	31.869537073519833	36.0	30.0	40.0	8.0	41.0
50	31.779350037391144	36.0	30.0	40.0	8.0	41.0
51	30.897453783742492	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	5.0
12	3.0
13	9.0
14	20.0
15	27.0
16	44.0
17	91.0
18	199.0
19	382.0
20	692.0
21	1082.0
22	1812.0
23	3154.0
24	5942.0
25	11251.0
26	17222.0
27	19212.0
28	16905.0
29	13911.0
30	12374.0
31	12267.0
32	13443.0
33	16477.0
34	25946.0
35	33288.0
36	41216.0
37	52381.0
38	89181.0
39	108857.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.790505061876313	4.3118421370043665	35.543699391288264	37.35395340983106
2	40.606379813607155	4.581018164858758	42.02020729971615	12.792394721817935
3	18.728540298003395	4.53538488754513	60.93610537065479	15.799969443796687
4	14.833026431115865	4.722340605173648	61.891790834747226	18.55284212896326
5	13.616206045303592	5.661340774036876	61.17874574826513	19.5437074323944
6	17.128963260186072	6.463642138612588	63.53056826497053	12.876826336230812
7	66.21549360330008	2.35041532313185	28.256044901536654	3.178046172031425
8	67.08795361889982	2.71407434806732	26.974895666647903	3.2230763663849595
9	62.22187824157091	4.800138307025515	28.659105346531472	4.318878104872106
10	29.11986072804175	21.066893961933403	35.50731338602938	14.305931923995466
11	21.74114071131625	20.67529209318034	40.80037149910341	16.783195696399996
12	19.16698160999027	18.405287831394087	45.28811283280128	17.139617725814364
13	18.69416456927815	18.253310925450904	46.91402449320928	16.13850001206166
14	17.497849004109007	19.52722316481855	46.241385965053354	16.733541866019088
15	16.54839539727085	19.634973987021656	46.7777277442285	17.038902871479
16	17.293604908291186	18.74281326139224	46.38170326710142	17.581878563215156
17	17.4481951737281	18.566109954085285	45.314648483045325	18.671046389141292
18	17.867538858645396	18.381968623603864	45.681322922781256	18.069169594969484
19	18.52510031279903	20.365910534653146	44.17321346724455	16.93577568530327
20	20.06838960767443	20.869283778676596	42.78069491239215	16.281631701256824
21	19.949582264536307	21.481412983169964	42.51051374627094	16.058491006022788
22	19.13843568321258	19.886459581380013	42.63334164247634	18.341763092931064
23	17.85728644832383	21.410048166225746	42.13901464285427	18.59365074259615
24	17.56298196379894	20.60995810583704	43.54399691221524	18.283063018148777
25	17.872162494672768	21.664147120077835	41.942610625517645	18.521079759731748
26	17.12634990069234	22.128319971695305	42.49684386584219	18.248486261770168
27	17.390701264865996	21.320992915785496	42.96121774511302	18.327088074235494
28	16.723892538657616	22.1691285853282	42.88120873907415	18.225770136940035
29	17.936491343749246	21.613689179083476	41.92250786018125	18.527311616986033
30	18.71487041757464	21.488046895730978	42.0690570194836	17.72802566721078
31	18.61777406099983	23.537121766470197	39.50253696898545	18.34256720354452
32	18.208481758750732	23.767901512532063	38.48513601531027	19.538480713406937
33	19.068679087495276	23.8280087808879	36.732375905629574	20.370936225987247
34	18.890568586614776	24.24091958089755	35.92746118156013	20.941050650927544
35	18.909867241337718	26.164754223590997	34.357234181133954	20.568144353937328
36	19.13240485361166	27.265179598105515	33.39370863856032	20.2087069097225
37	19.500285459267776	27.302771769284583	33.35993599279517	19.837006778652473
38	19.279959151180837	27.232412090607184	32.25669623113355	21.230932527078426
39	19.91178906570388	26.645411342784314	32.03737506131343	21.405424530198374
40	21.024276099420234	25.575542171581123	33.09960518168879	20.30057654730985
41	19.34308183433713	26.339849309671038	33.10442984536952	21.2126390106223
42	19.420678508535634	27.613761549038685	32.52185170592067	20.443708236505014
43	19.186883347673305	26.551129373356595	33.934271998456104	20.32771528051399
44	19.44661107581959	26.164754223590997	33.383255200585396	21.00537950000402
45	20.46059455938759	25.311793890367557	32.69594165373389	21.531669896510962
46	20.11341980202797	25.815971245004466	33.28555576105049	20.78505319191708
47	19.28458278720821	26.62410241152773	33.81265026817089	20.27866453309317
48	18.9613303205989	25.802904447535802	34.87528244385298	20.36048278801232
49	19.79981666278013	24.93305779142979	34.31320912504724	20.95391642074284
50	19.052596875226154	24.457024308263843	34.37572872524344	22.114650091266554
51	18.707030339093443	23.98038774213781	33.75033169562805	23.562250223140698
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	678.0
1	1386.5
2	2095.0
3	18078.5
4	34062.0
5	22289.5
6	10517.0
7	10114.0
8	9711.0
9	9680.0
10	9649.0
11	9253.5
12	8858.0
13	8481.5
14	8105.0
15	7684.0
16	7263.0
17	6937.5
18	6612.0
19	6288.0
20	5964.0
21	5794.5
22	5625.0
23	5428.0
24	5231.0
25	5452.5
26	5866.5
27	6059.0
28	6373.0
29	6687.0
30	7207.5
31	7728.0
32	8476.5
33	9225.0
34	10177.5
35	11130.0
36	11760.0
37	12390.0
38	13546.0
39	14702.0
40	15972.5
41	17243.0
42	19034.0
43	20825.0
44	22109.0
45	23393.0
46	26677.5
47	29962.0
48	32181.5
49	34401.0
50	34087.5
51	33774.0
52	31824.0
53	29874.0
54	26733.5
55	23593.0
56	21931.0
57	20269.0
58	19240.5
59	18212.0
60	16800.5
61	15389.0
62	13782.5
63	12176.0
64	11026.0
65	9876.0
66	8177.0
67	6478.0
68	5489.5
69	4501.0
70	3915.5
71	3330.0
72	2843.5
73	2357.0
74	1954.5
75	1302.5
76	1053.0
77	883.5
78	714.0
79	489.5
80	265.0
81	205.0
82	145.0
83	102.5
84	60.0
85	42.0
86	24.0
87	15.0
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.50713813675674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.26414622453164	31.875133302622427
2	8.898200894807895	7.74270510998039
3	4.59123256173523	5.992541678441705
4	2.930939967150263	5.1006724008539095
5	2.162051453167116	4.703233561585868
6	1.67472360984773	4.371745886071985
7	1.2422531438509217	3.7832815391239665
8	0.9976173473152841	3.4722780589816713
9	0.7779654467465518	3.0462345141503304
>10	3.387131798092892	22.212306667956224
>50	0.0510134012772142	1.5108381687427783
>100	0.020869118703620557	1.7263263049454816
>500	0.0013912745802413704	0.3543044637078384
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.637581934137901E-4	4.108398342835424
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20362	4.093325077797702	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC	713	0.14333271684852966	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	529	0.1063436286295543	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.10332821382909434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0102765336399675E-4	0.0	0.0	0.07659153593168276	0.0
2	2.0102765336399675E-4	0.0	0.0	0.23861982454306416	0.0
3	2.0102765336399675E-4	0.0	0.0	0.372504241683486	0.0
4	2.0102765336399675E-4	0.0	0.0	0.5731298397407547	0.0
5	2.0102765336399675E-4	0.0	0.0	1.0765030837642027	0.0
6	2.0102765336399675E-4	0.0	0.0	1.798393386994315	0.0
7	2.0102765336399675E-4	0.0	0.0	2.399064015245937	0.0
8	2.0102765336399675E-4	0.0	0.0	3.224885615265236	0.0
9	2.0102765336399675E-4	0.0	0.0	3.7387122972636115	0.0
10	2.0102765336399675E-4	0.0	0.0	4.532570500398035	0.0
11	2.0102765336399675E-4	0.0	0.0	5.97534596859144	0.0
12	2.0102765336399675E-4	0.0	0.0	7.150754657810729	0.0
13	2.0102765336399675E-4	0.0	0.0	7.555825379339182	0.0
14	2.0102765336399675E-4	0.0	0.0	7.7188588062173835	0.0
15	2.0102765336399675E-4	0.0	0.0	7.939989224917779	0.0
16	4.020553067279935E-4	0.0	0.0	8.40174974469488	0.0
17	4.020553067279935E-4	0.0	0.0	8.951761404298775	0.0
18	4.020553067279935E-4	0.0	0.0	9.494938123688295	0.0
19	4.020553067279935E-4	0.0	0.0	9.84975193187575	0.0
20	4.020553067279935E-4	0.0	0.0	10.165968430617315	0.0
21	4.020553067279935E-4	0.0	0.0	10.527617179019146	0.0
22	4.020553067279935E-4	0.0	0.0	10.878812489446048	0.0
23	4.020553067279935E-4	0.0	0.0	11.157436817008549	0.0
24	4.020553067279935E-4	0.0	0.0	11.342784313410153	0.0
25	4.020553067279935E-4	0.0	0.0	11.49456019169997	0.0
26	4.020553067279935E-4	0.0	0.0	11.639099074468684	0.0
27	4.020553067279935E-4	0.0	0.0	11.766751634354822	0.0
28	4.020553067279935E-4	0.0	0.0	11.885558977492943	0.0
29	4.020553067279935E-4	0.0	0.0	12.013814620339174	0.0
30	4.020553067279935E-4	0.0	0.0	12.161368917908348	0.0
31	4.020553067279935E-4	0.0	0.0	12.286408118300754	0.0
32	4.020553067279935E-4	0.0	0.0	12.451652849365958	0.0
33	4.020553067279935E-4	0.0	0.0	12.59860406397504	0.0
34	4.020553067279935E-4	0.0	0.0	12.73892136602311	0.0
35	4.020553067279935E-4	0.0	0.0	12.8701924236698	0.0
36	4.020553067279935E-4	0.0	0.0	13.00548403438377	0.0
37	4.020553067279935E-4	0.0	0.0	13.139770506830919	0.0
38	4.020553067279935E-4	0.0	0.0	13.303608044322576	0.0
39	6.030829600919903E-4	0.0	0.0	13.469857913654602	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	30	2.1630094E-6	45.000004	17
CGCACGA	20	7.0294226E-4	45.000004	39
AATCGTT	20	7.0294226E-4	45.000004	22
TAATCGT	20	7.0294226E-4	45.000004	21
TAACGTC	25	3.8876035E-5	45.0	32
CGGTAGG	35	1.2100645E-7	45.0	31
CGTTTTT	10645	0.0	43.77407	1
GCTACGA	65	0.0	41.53846	10
TACGGGA	45	1.9244908E-8	40.0	4
CGGGAAT	85	0.0	39.705883	6
CTTCGTG	40	3.4529512E-7	39.375004	1
GCGATTC	115	0.0	39.130432	9
GCGATGT	190	0.0	39.078945	9
TGGTTCG	35	6.2411455E-6	38.571426	1
CGAATAT	70	0.0	38.571426	14
ATAACGT	35	6.2411455E-6	38.571426	31
GTTTTTT	12640	0.0	38.23576	2
TTACACG	165	0.0	38.181816	34
AGCTACG	65	9.094947E-12	38.07692	9
CGTGCGG	60	1.546141E-10	37.500004	2
>>END_MODULE