FastQCFastQC Report
Sat 14 Jan 2017
SRR2933680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192364
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56602.942338483292092No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT10540.5479195691501528TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC10040.5219271797217775TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC8680.45122788047659645TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC8420.4377118379738413TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC7060.36701253872866024No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC3780.19650246407851782No Hit
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG3670.19078413840427522TruSeq Adapter, Index 16 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2570.1336008816618494No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2570.1336008816618494No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG2330.12112453473622922No Hit
TGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG2160.1122871223305816TruSeq Adapter, Index 16 (95% over 21bp)
GTGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC2120.11020773117631157No Hit
TGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2100.10916803559917657No Hit
GCTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC2030.105529101079204No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC1990.10344970992493398No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.10033062319352894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC207.01863E-445.021
CTGCTCG207.01863E-445.01
TTAGGGT406.7648216E-945.04
CGTGGGA406.7648216E-945.04
TCGTTCA207.01863E-445.016
ACACGAC207.01863E-445.026
GGATGTC253.8786617E-545.08
CCGATCC207.01863E-445.042
GCTGGAT406.7648216E-945.04
GACACGA207.01863E-445.025
CTTCAGG253.8786617E-545.02
TATGGGT253.8786617E-545.04
TATGGGA453.8198777E-1045.04
GGGTACG207.01863E-445.07
TCGGGTA253.8786617E-545.05
CGACCAA207.01863E-445.029
ATGCTGG207.01863E-445.02
AGGGTTG207.01863E-445.06
CACGACC207.01863E-445.027
CCTCGTT207.01863E-445.014
Produced by FastQC (version 0.11.5)