##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933680.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192364
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.695587531970638	31.0	31.0	34.0	30.0	34.0
2	31.87734711276538	33.0	31.0	34.0	30.0	34.0
3	32.05382504002828	33.0	31.0	34.0	30.0	34.0
4	35.6874727079911	37.0	35.0	37.0	33.0	37.0
5	35.7044561352436	37.0	35.0	37.0	35.0	37.0
6	35.65704081844836	37.0	35.0	37.0	35.0	37.0
7	35.75350897257283	37.0	35.0	37.0	35.0	37.0
8	35.51547586866565	37.0	35.0	37.0	33.0	37.0
9	37.30658543178557	39.0	37.0	39.0	34.0	39.0
10	36.65864714811503	39.0	35.0	39.0	32.0	39.0
11	36.745030255141295	39.0	35.0	39.0	32.0	39.0
12	36.95680064877004	39.0	37.0	39.0	33.0	39.0
13	36.93226903162754	39.0	37.0	39.0	33.0	39.0
14	37.8372408558774	39.0	37.0	41.0	33.0	41.0
15	37.82787319872741	39.0	37.0	41.0	33.0	41.0
16	37.9450936765715	39.0	37.0	41.0	33.0	41.0
17	37.98968621987482	39.0	37.0	41.0	33.0	41.0
18	37.86566613295627	39.0	37.0	40.0	33.0	41.0
19	37.69214613961032	39.0	37.0	40.0	33.0	41.0
20	37.30365349025805	39.0	35.0	40.0	33.0	41.0
21	37.148822024911105	39.0	35.0	40.0	32.0	41.0
22	37.19906011519827	39.0	35.0	40.0	33.0	41.0
23	37.164692977896074	39.0	35.0	40.0	32.0	41.0
24	37.06087417604125	39.0	35.0	40.0	32.0	41.0
25	36.96914703374852	38.0	35.0	40.0	32.0	41.0
26	36.96474912145724	38.0	35.0	40.0	32.0	41.0
27	36.91276954107837	38.0	35.0	40.0	32.0	41.0
28	36.859947807282026	38.0	35.0	40.0	32.0	41.0
29	36.8218169719906	39.0	35.0	40.0	32.0	41.0
30	36.75051464931068	39.0	35.0	40.0	31.0	41.0
31	36.51621405252542	38.0	35.0	40.0	31.0	41.0
32	36.26148863612734	38.0	35.0	40.0	30.0	41.0
33	36.09300596785261	39.0	35.0	40.0	30.0	41.0
34	35.96532615250255	39.0	35.0	40.0	29.0	41.0
35	35.81801168617829	39.0	35.0	40.0	27.0	41.0
36	35.7313478613462	39.0	35.0	40.0	27.0	41.0
37	35.51338608055561	39.0	35.0	40.0	25.0	41.0
38	35.366175583789065	38.0	35.0	40.0	25.0	41.0
39	35.30586804183735	39.0	35.0	40.0	24.0	41.0
40	35.27224948535069	39.0	35.0	40.0	24.0	41.0
41	35.19716267077	38.0	35.0	40.0	24.0	41.0
42	35.187602669938244	39.0	35.0	40.0	24.0	41.0
43	35.05162608388264	38.0	35.0	40.0	23.0	41.0
44	34.942795949346035	38.0	35.0	40.0	23.0	41.0
45	34.91329978582271	38.0	35.0	40.0	23.0	41.0
46	34.58629993138009	38.0	34.0	40.0	22.0	41.0
47	34.36281736707492	38.0	33.0	40.0	21.0	41.0
48	34.35514961219355	38.0	34.0	40.0	21.0	41.0
49	34.50863987024599	38.0	34.0	40.0	21.0	41.0
50	34.47125241729222	38.0	34.0	40.0	20.0	41.0
51	33.39134141523362	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	1.0
13	6.0
14	3.0
15	5.0
16	9.0
17	21.0
18	46.0
19	103.0
20	139.0
21	272.0
22	429.0
23	697.0
24	1225.0
25	2081.0
26	3136.0
27	3806.0
28	3777.0
29	3940.0
30	4105.0
31	4966.0
32	5999.0
33	7880.0
34	11215.0
35	14852.0
36	20029.0
37	27399.0
38	45012.0
39	31204.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.059782495685262	4.9120417541743775	44.744858705371065	25.283317044769294
2	31.66496849722401	5.363789482439542	42.9716578985673	19.999584121769146
3	20.546983843130732	5.341436027531139	52.71204591295668	21.399534216381443
4	16.11060281549562	5.363789482439542	54.06728909775218	24.458318604312655
5	13.294067497036869	7.190534611465763	55.41577426129629	24.099623630201076
6	17.782953151317294	8.262980599280532	59.31827161007257	14.635794639329605
7	68.05535339252667	2.642906157077208	23.094238007111517	6.207502443284606
8	65.77893992638955	3.4029236239629035	22.19126239836976	8.626874051277786
9	59.19766692312491	6.127965731633777	26.048013141752097	8.62635420348922
10	27.284731030754195	21.475951841300866	32.677112141564955	18.56220498637999
11	16.31958162649976	19.324301844419953	38.4183111185045	25.93780541057578
12	15.528373292300016	14.127903349899148	45.89424216589383	24.44948119190701
13	20.48408226071406	15.490424403734588	48.267347320704495	15.758146014846853
14	18.104738932440583	18.057432783680937	45.034933771391735	18.802894512486745
15	11.826017342122226	18.145806907737413	47.48705579006467	22.54111996007569
16	10.330415254413507	18.342309371815933	43.70412343265892	27.62315194111164
17	10.016427190118733	18.51177975088894	42.32860618410929	29.143186874883035
18	11.572851469089851	16.76249194235928	46.78110249319	24.88355409536088
19	11.833815058950739	19.908610758769832	48.16597700193383	20.091597180345595
20	11.97573350522967	24.377222349296126	44.08725125283317	19.559792892641035
21	10.643363623131147	26.74097024391258	43.76182653718991	18.85383959576636
22	8.84728951363041	24.137572518766508	40.803892620240795	26.211245347362294
23	9.617184088498886	24.40737352103304	37.74978686240669	28.225655528061385
24	11.413778045788193	22.872263001393193	37.77473955625793	27.939219396560688
25	10.010189016655923	25.939884801730052	34.589112307916245	29.460813873697784
26	9.515293921939657	26.21176519515086	38.2696346509742	26.00330623193529
27	12.799692250109167	24.766068495144623	40.31523569898734	22.119003555758873
28	10.848703499615313	20.66394959555842	40.44103886382067	28.04630804100559
29	12.481025555717286	19.251523154020504	37.721715081824044	30.545736208438168
30	19.69027468757148	20.661350356615582	34.986276018381815	24.66209893743112
31	18.577800420037015	27.793662015761782	28.1726310536275	25.455906510573705
32	14.454887608908113	27.598719095048967	28.10349129774802	29.842901998294902
33	17.741365328231893	20.412863113680316	29.594414755359633	32.25135680272816
34	18.59339585369404	16.796801896404734	28.918612630221872	35.691189619679356
35	13.601297540080266	17.244910690149922	30.877399097544238	38.276392672225576
36	16.1589486598324	16.65748268906864	37.26944750577031	29.914121145328647
37	20.757002349712003	19.459982117236073	36.6840988958433	23.098916637208625
38	20.882285666756772	24.74839367033333	30.479195691501527	23.890124971408373
39	17.68626146264374	20.637957206130046	28.95864090994157	32.71714042128465
40	16.65592314570294	14.915472749578923	30.877918945332805	37.55068515938533
41	13.817554220124348	14.780832172339938	37.553804246116734	33.84780936141898
42	17.384229897486016	14.360795159177393	45.35619970472646	22.898775238610135
43	18.38025826038136	15.31003722110166	44.97567112349504	21.334033395021937
44	19.023309974839368	16.391840469110644	33.660664157534676	30.924185398515313
45	22.25104489405502	13.876816868021043	29.63600257844503	34.236135659478904
46	23.30633590484706	14.290095859932212	36.048325050425234	26.35524318479549
47	15.051153022395042	19.261920109791852	42.00630055519744	23.680626312615665
48	14.766276434260048	18.525295793391695	43.779501362001206	22.92892641034705
49	21.49674575284357	12.26944750577031	42.31145120708656	23.92235553429956
50	20.73516874259217	9.836559855274375	36.646669855066435	32.78160154706702
51	17.377471876234637	9.581314591087729	33.3050882701545	39.73612526252313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	281.0
1	519.0
2	757.0
3	4620.5
4	8484.0
5	5232.5
6	1981.0
7	1891.5
8	1802.0
9	1772.0
10	1742.0
11	1677.0
12	1612.0
13	1512.0
14	1412.0
15	1322.0
16	1232.0
17	1135.5
18	1039.0
19	957.5
20	876.0
21	823.0
22	770.0
23	743.5
24	717.0
25	662.5
26	637.5
27	667.0
28	751.0
29	835.0
30	958.5
31	1082.0
32	1116.0
33	1150.0
34	1350.0
35	1550.0
36	1762.5
37	1975.0
38	2371.0
39	2767.0
40	3697.0
41	4627.0
42	6594.5
43	8562.0
44	10752.0
45	12942.0
46	17888.0
47	22834.0
48	28678.0
49	34522.0
50	34244.5
51	33967.0
52	27240.5
53	20514.0
54	14941.0
55	9368.0
56	6615.5
57	3863.0
58	3002.0
59	2141.0
60	1857.0
61	1573.0
62	1417.0
63	1261.0
64	1065.5
65	870.0
66	767.0
67	664.0
68	524.5
69	385.0
70	357.0
71	329.0
72	295.0
73	261.0
74	206.0
75	122.5
76	94.0
77	74.5
78	55.0
79	38.0
80	21.0
81	14.5
82	8.0
83	8.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192364.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.49385539913913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07618498538072	37.90886028570834
2	12.64361899271095	12.768501382795117
3	5.006588971708602	7.584059387411365
4	2.3977679858337106	4.842901998294899
5	1.229255034386196	3.1034912977480196
6	0.7875880245439196	2.3861013495248593
7	0.5106453074167113	1.8049115219063858
8	0.37474776592678005	1.5137967603085816
9	0.27694271712720836	1.2585514961219355
>10	1.533994975909072	14.14869726144185
>50	0.11324795124160936	3.841155309725312
>100	0.04324012683770539	3.5708344596702086
>500	0.0030885804884075276	1.255952257179098
>1k	0.002059053658938352	1.0698467488719303
>5k	0.001029526829469176	2.942338483292092
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5660	2.942338483292092	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1054	0.5479195691501528	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	1004	0.5219271797217775	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	868	0.45122788047659645	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	842	0.4377118379738413	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	706	0.36701253872866024	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	378	0.19650246407851782	No Hit
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	367	0.19078413840427522	TruSeq Adapter, Index 16 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	257	0.1336008816618494	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	257	0.1336008816618494	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	233	0.12112453473622922	No Hit
TGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	216	0.1122871223305816	TruSeq Adapter, Index 16 (95% over 21bp)
GTGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	212	0.11020773117631157	No Hit
TGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	210	0.10916803559917657	No Hit
GCTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	203	0.105529101079204	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	199	0.10344970992493398	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	193	0.10033062319352894	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5926264789669585	0.0
2	0.0	0.0	0.0	2.1906385810234763	0.0
3	0.0	0.0	0.0	3.1118088623650997	0.0
4	0.0	0.0	0.0	4.619367449210871	0.0
5	0.0	0.0	0.0	7.735854941673078	0.0
6	0.0	0.0	0.0	10.397995466927284	0.0
7	0.0	0.0	0.0	12.890665613108482	0.0
8	0.0	0.0	0.0	16.633569690794534	0.0
9	0.0	0.0	0.0	18.35374602316442	0.0
10	5.198477885675074E-4	0.0	0.0	22.392443492545382	0.0
11	5.198477885675074E-4	0.0	0.0	31.627539456447153	0.0
12	5.198477885675074E-4	0.0	0.0	40.05167287018361	0.0
13	5.198477885675074E-4	0.0	0.0	42.799588280551454	0.0
14	5.198477885675074E-4	0.0	0.0	43.672932565344865	0.0
15	5.198477885675074E-4	0.0	0.0	44.960075689838014	0.0
16	5.198477885675074E-4	0.0	0.0	48.21640223742488	0.0
17	5.198477885675074E-4	0.0	0.0	52.181281320829264	0.0
18	5.198477885675074E-4	0.0	0.0	56.01515876151463	0.0
19	5.198477885675074E-4	0.0	0.0	58.21723399388659	0.0
20	5.198477885675074E-4	0.0	0.0	60.14846852841488	0.0
21	5.198477885675074E-4	0.0	0.0	62.44047742820902	0.0
22	5.198477885675074E-4	0.0	0.0	64.27242103512091	0.0
23	5.198477885675074E-4	0.0	0.0	65.56164355076834	0.0
24	5.198477885675074E-4	0.0	0.0	66.27435486889439	0.0
25	5.198477885675074E-4	0.0	0.0	66.71934457590818	0.0
26	5.198477885675074E-4	0.0	0.0	67.04632883491713	0.0
27	5.198477885675074E-4	0.0	0.0	67.32288785843505	0.0
28	5.198477885675074E-4	0.0	0.0	67.59112931733588	0.0
29	5.198477885675074E-4	0.0	0.0	67.82817990892266	0.0
30	5.198477885675074E-4	0.0	0.0	68.05691293589237	0.0
31	5.198477885675074E-4	0.0	0.0	68.2404192052567	0.0
32	5.198477885675074E-4	0.0	0.0	68.41196897548397	0.0
33	5.198477885675074E-4	0.0	0.0	68.56428437753426	0.0
34	5.198477885675074E-4	0.0	0.0	68.693206629099	0.0
35	5.198477885675074E-4	0.0	0.0	68.82524796739514	0.0
36	5.198477885675074E-4	0.0	0.0	68.95988854463413	0.0
37	5.198477885675074E-4	0.0	0.0	69.08621155725604	0.0
38	5.198477885675074E-4	0.0	0.0	69.22345137343785	0.0
39	5.198477885675074E-4	0.0	0.0	69.37992555779668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.01863E-4	45.0	21
CTGCTCG	20	7.01863E-4	45.0	1
TTAGGGT	40	6.7648216E-9	45.0	4
CGTGGGA	40	6.7648216E-9	45.0	4
TCGTTCA	20	7.01863E-4	45.0	16
ACACGAC	20	7.01863E-4	45.0	26
GGATGTC	25	3.8786617E-5	45.0	8
CCGATCC	20	7.01863E-4	45.0	42
GCTGGAT	40	6.7648216E-9	45.0	4
GACACGA	20	7.01863E-4	45.0	25
CTTCAGG	25	3.8786617E-5	45.0	2
TATGGGT	25	3.8786617E-5	45.0	4
TATGGGA	45	3.8198777E-10	45.0	4
GGGTACG	20	7.01863E-4	45.0	7
TCGGGTA	25	3.8786617E-5	45.0	5
CGACCAA	20	7.01863E-4	45.0	29
ATGCTGG	20	7.01863E-4	45.0	2
AGGGTTG	20	7.01863E-4	45.0	6
CACGACC	20	7.01863E-4	45.0	27
CCTCGTT	20	7.01863E-4	45.0	14
>>END_MODULE