FastQCFastQC Report
Sat 14 Jan 2017
SRR2933679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191945
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55962.915418479251869No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT10870.5663080569954935TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC9900.5157727474015994TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC8860.46159055979577485TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC8640.45012894318685037TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC7360.38344317382583554No Hit
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG3670.19120060433978484TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC3590.18703274375472143No Hit
TTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2860.1490010159160176No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG2580.13441350386829562No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2340.1219099221131053No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC2250.11722107895490896No Hit
TGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG2130.11096928807731381TruSeq Adapter, Index 16 (95% over 21bp)
TGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT2070.10784339263851624No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG2010.10471749719971866TruSeq Adapter, Index 16 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC1970.10263356690718695No Hit
GTGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC1970.10263356690718695No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.10107061918778817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG302.1560245E-645.0000045
TTAGGGA302.1560245E-645.0000044
GTCTGGT302.1560245E-645.0000043
CTCGGGA302.1560245E-645.0000044
ACTAATC302.1560245E-645.00000410
TAGGGCG302.1560245E-645.0000045
CTGGATA302.1560245E-645.0000045
GACTAAT302.1560245E-645.0000049
CTTCGTG253.8786286E-545.01
GTGTTAG207.0185907E-445.01
CATGGGA253.8786286E-545.04
CTGTCGG207.0185907E-445.02
TCTTGCG406.7648216E-945.01
ACACGCG207.0185907E-445.036
GAGCTAG207.0185907E-445.01
TGCTTCG207.0185907E-445.01
AAGAGCA207.0185907E-445.035
CGTGCGG207.0185907E-445.02
AACCCGA207.0185907E-445.024
CGGTTTG253.8786286E-545.01
Produced by FastQC (version 0.11.5)