##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933679.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191945
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60654354111855	31.0	31.0	34.0	30.0	34.0
2	31.790856755841517	33.0	31.0	34.0	30.0	34.0
3	32.00075021490531	33.0	31.0	34.0	30.0	34.0
4	35.59387845476569	37.0	35.0	37.0	33.0	37.0
5	35.680606423715126	37.0	35.0	37.0	35.0	37.0
6	35.67481830732762	37.0	35.0	37.0	35.0	37.0
7	35.668837427388056	37.0	35.0	37.0	35.0	37.0
8	35.48785850113314	37.0	35.0	37.0	33.0	37.0
9	37.27326056943395	39.0	37.0	39.0	34.0	39.0
10	36.72132121180547	39.0	37.0	39.0	32.0	39.0
11	36.7681888040845	39.0	37.0	39.0	32.0	39.0
12	36.93654953241814	39.0	37.0	39.0	33.0	39.0
13	36.93426241892209	39.0	37.0	39.0	33.0	39.0
14	37.9082393393941	39.0	37.0	41.0	33.0	41.0
15	37.86156972049285	39.0	37.0	41.0	33.0	41.0
16	38.05338508426893	39.0	37.0	41.0	33.0	41.0
17	38.126916564640915	40.0	37.0	41.0	33.0	41.0
18	38.077543046185106	39.0	37.0	40.0	34.0	41.0
19	37.945823022219905	39.0	37.0	40.0	34.0	41.0
20	37.53814894891766	39.0	35.0	40.0	33.0	41.0
21	37.43859959884342	39.0	35.0	40.0	33.0	41.0
22	37.456891296986115	39.0	35.0	40.0	33.0	41.0
23	37.34486962411108	39.0	35.0	40.0	33.0	41.0
24	37.22799239365443	39.0	35.0	40.0	33.0	41.0
25	37.16953293912319	39.0	35.0	40.0	33.0	41.0
26	37.217338300033866	39.0	35.0	40.0	33.0	41.0
27	37.18667326577926	39.0	35.0	40.0	33.0	41.0
28	37.19770246685248	39.0	35.0	40.0	33.0	41.0
29	37.10212821381125	39.0	35.0	40.0	33.0	41.0
30	36.91163614577093	39.0	35.0	40.0	32.0	41.0
31	36.67833493969627	39.0	35.0	40.0	31.0	41.0
32	36.555622704420536	39.0	35.0	40.0	31.0	41.0
33	36.240318841334755	39.0	35.0	40.0	30.0	41.0
34	36.10510302430384	39.0	35.0	40.0	29.0	41.0
35	36.00764281434786	39.0	35.0	40.0	29.0	41.0
36	35.89442809138034	39.0	35.0	40.0	27.0	41.0
37	35.75269999218526	39.0	35.0	40.0	26.0	41.0
38	35.59615514861028	39.0	35.0	40.0	26.0	41.0
39	35.630592096694365	39.0	35.0	40.0	25.0	41.0
40	35.54450493630988	39.0	35.0	40.0	25.0	41.0
41	35.501320690822894	39.0	35.0	40.0	25.0	41.0
42	35.39576962150616	39.0	35.0	40.0	24.0	41.0
43	35.295516944958194	39.0	35.0	40.0	24.0	41.0
44	35.173982130297745	39.0	35.0	40.0	23.0	41.0
45	35.12120659563938	38.0	35.0	40.0	23.0	41.0
46	34.791956029070825	38.0	34.0	40.0	23.0	41.0
47	34.6997264841491	38.0	34.0	40.0	23.0	41.0
48	34.6559066399229	38.0	34.0	40.0	22.0	41.0
49	34.78159889551694	38.0	34.0	40.0	22.0	41.0
50	34.62880512646852	38.0	34.0	40.0	22.0	41.0
51	33.76813149600146	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	5.0
13	13.0
14	7.0
15	10.0
16	11.0
17	13.0
18	50.0
19	97.0
20	116.0
21	252.0
22	405.0
23	652.0
24	1174.0
25	2015.0
26	3092.0
27	3614.0
28	3576.0
29	3667.0
30	4089.0
31	4749.0
32	5731.0
33	7422.0
34	10352.0
35	14310.0
36	19112.0
37	25277.0
38	43975.0
39	38150.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.344760217770716	5.1212586938966895	44.2434030581677	25.29057803016489
2	31.19747844434604	5.38643882362135	43.24415848289875	20.171924249133866
3	20.65122821641616	5.516684466904582	52.42074552606215	21.411341790617104
4	16.256740212039908	5.513558571465785	53.58930943759931	24.640391778894998
5	13.518976790226366	7.2843783375446085	55.06004324155357	24.136601630675454
6	18.02755997811873	8.227877777488343	59.02836750110709	14.716194743285838
7	68.19818177081977	2.783088905676105	22.819036703222277	6.199692620281851
8	65.9616035843601	3.4520305295787854	21.903670322227722	8.68269556383339
9	59.291984683112354	6.190314933965459	25.850634296282788	8.667066086639402
10	27.489124488785848	21.510849462085492	32.2300659042955	18.769960144833156
11	16.286436218708484	19.326369532939122	38.314100393341846	26.073093855010548
12	15.77691526218448	14.243142566881136	45.69069264633098	24.289249524603402
13	20.689259944254864	15.627914246268462	47.96165568261742	15.721170126859258
14	18.10362343379614	18.355778999192477	44.60131808591002	18.939279481101355
15	11.81015395035036	18.341191487144755	47.15569564198078	22.69295892052411
16	10.598869467816302	18.209382896142124	43.53955560186512	27.65219203417646
17	10.339941128969237	18.506342962827894	41.85209304748756	29.30162286071531
18	11.727838703795358	16.759488395113184	46.680038552710414	24.832634348381045
19	12.116491703352523	19.993227226549273	47.912162338169786	19.97811873192842
20	12.128995285107713	24.447628226835814	43.84693532001355	19.57644116804293
21	10.844773242335044	26.722759123707313	43.59165385917841	18.84081377477923
22	9.103649482924796	24.102737763421814	40.495975409622545	26.297637344030843
23	9.878871551746595	24.528901508244548	37.32267055666988	28.26955638333898
24	11.49495949360494	23.184245486988463	37.57534710463935	27.74544791476725
25	10.23053478861132	26.01422282424653	34.23584881085728	29.519393576284873
26	9.530334210320666	26.400791893511162	38.05308812420225	26.015785771965927
27	12.965172314986065	24.700304774805282	40.28080960691865	22.053713303290003
28	10.864049597540962	20.8606632108156	40.25319753054261	28.022089661100836
29	12.543697413321524	19.167990830706714	37.786345046758186	30.50196670921358
30	19.98801740081794	20.868477949412593	34.83862564797208	24.30487900179739
31	18.705879288337805	27.888197139805676	27.973638281799474	25.43228529005705
32	14.526036104092318	27.77774883430149	27.7714970434239	29.92471801818229
33	17.873349136471383	20.21151892469197	29.67620933079789	32.23892260803876
34	18.78142176144208	16.822006303889133	28.598296386985854	35.79827554768293
35	13.671624684154315	17.300268306025163	30.930214384328846	38.097892625491674
36	16.252051368881713	16.817838443304073	37.14814139467035	29.78196879314387
37	20.824715413269427	19.558727760556412	36.45315064211102	23.163406184063142
38	20.870040897131993	24.73677355492459	30.493630988043453	23.899554559899972
39	17.998905936596422	20.714267107765245	28.78220323530178	32.504623720336554
40	16.644351246450807	14.85373414259293	30.827059834848527	37.67485477610774
41	13.903461929198468	14.908437312771888	37.45239521737998	33.73570554064966
42	17.464377816562035	14.39266456537029	45.322357967125996	22.820599650941674
43	18.526140300606944	15.305426033499177	44.82481960978405	21.34361405610982
44	18.954908958295345	16.59329495428378	33.57941076870979	30.87238531871109
45	22.425694860506916	13.925864179843185	29.555862356404177	34.09257860324572
46	23.358774648987993	14.464560160462634	36.184844616947565	25.991820573601814
47	15.09182317851468	19.51809111985204	41.85209304748756	23.53799265414572
48	14.769334965745395	18.67670426424236	43.643752116491704	22.910208653520538
49	21.760921097189296	12.375941024772722	42.12196202037042	23.74117585766756
50	20.65487509442809	9.985672979238844	36.52869311521529	32.83075881111777
51	17.494073823230615	9.651202167287504	33.14074344213186	39.71398056735002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	487.0
2	703.0
3	4531.5
4	8360.0
5	5191.5
6	2023.0
7	1916.5
8	1810.0
9	1776.0
10	1742.0
11	1655.5
12	1569.0
13	1465.0
14	1361.0
15	1251.5
16	1142.0
17	1052.5
18	963.0
19	880.5
20	798.0
21	752.0
22	706.0
23	706.0
24	706.0
25	648.0
26	640.0
27	690.0
28	759.5
29	829.0
30	959.0
31	1089.0
32	1170.5
33	1252.0
34	1429.5
35	1607.0
36	1841.0
37	2075.0
38	2482.0
39	2889.0
40	3752.5
41	4616.0
42	6552.5
43	8489.0
44	10675.5
45	12862.0
46	17788.0
47	22714.0
48	28342.0
49	33970.0
50	33961.5
51	33953.0
52	27306.5
53	20660.0
54	15007.0
55	9354.0
56	6669.0
57	3984.0
58	3108.5
59	2233.0
60	1919.0
61	1605.0
62	1444.0
63	1283.0
64	1130.0
65	977.0
66	816.0
67	655.0
68	529.0
69	403.0
70	383.5
71	364.0
72	300.5
73	237.0
74	214.0
75	153.0
76	115.0
77	89.0
78	63.0
79	42.0
80	21.0
81	17.0
82	13.0
83	9.0
84	5.0
85	3.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	191945.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.93490322748704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28307097486882	38.34535934772982
2	12.488876614808678	12.72239443590612
3	4.942362965008643	7.5521633801349335
4	2.401628361308008	4.893068326864467
5	1.2826413820614317	3.266560733543463
6	0.8059979338631645	2.463205605772487
7	0.49198604846216004	1.7541483237385709
8	0.3886792066852824	1.5837870223241033
9	0.2792353248028476	1.280054182187606
>10	1.4780038254216659	13.620047409414154
>50	0.10944388188243476	3.754721404569017
>100	0.041936440721306784	3.4718278673578373
>500	0.004091360070371393	1.8109354242100602
>1k	0.0010228400175928483	0.5663080569954935
>5k	0.0010228400175928483	2.915418479251869
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5596	2.915418479251869	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1087	0.5663080569954935	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	990	0.5157727474015994	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	886	0.46159055979577485	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	864	0.45012894318685037	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	736	0.38344317382583554	No Hit
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	367	0.19120060433978484	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	359	0.18703274375472143	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	286	0.1490010159160176	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	258	0.13441350386829562	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	234	0.1219099221131053	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	225	0.11722107895490896	No Hit
TGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	213	0.11096928807731381	TruSeq Adapter, Index 16 (95% over 21bp)
TGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	207	0.10784339263851624	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	201	0.10471749719971866	TruSeq Adapter, Index 16 (95% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	197	0.10263356690718695	No Hit
GTGCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	197	0.10263356690718695	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	194	0.10107061918778817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010419651462658574	0.0	0.0	0.614238453723723	0.0
2	0.0010419651462658574	0.0	0.0	2.169892417098648	0.0
3	0.0010419651462658574	0.0	0.0	3.0946364844095964	0.0
4	0.0010419651462658574	0.0	0.0	4.581520748130975	0.0
5	0.0010419651462658574	0.0	0.0	7.673031337101774	0.0
6	0.0010419651462658574	0.0	0.0	10.252937039256038	0.0
7	0.0010419651462658574	0.0	0.0	12.627054625022794	0.0
8	0.0010419651462658574	0.0	0.0	16.357810831227695	0.0
9	0.0010419651462658574	0.0	0.0	18.03797962958139	0.0
10	0.0010419651462658574	0.0	0.0	22.03495793065722	0.0
11	0.0010419651462658574	0.0	0.0	31.334496861079998	0.0
12	0.0010419651462658574	0.0	0.0	39.673864909218786	0.0
13	0.0010419651462658574	0.0	0.0	42.35119435254891	0.0
14	0.0010419651462658574	0.0	0.0	43.22488212769283	0.0
15	0.0010419651462658574	0.0	0.0	44.478366198650654	0.0
16	0.0010419651462658574	0.0	0.0	47.65948579020032	0.0
17	0.0010419651462658574	0.0	0.0	51.67678241162833	0.0
18	0.0010419651462658574	0.0	0.0	55.40493370496757	0.0
19	0.0010419651462658574	0.0	0.0	57.60973195446612	0.0
20	0.0010419651462658574	0.0	0.0	59.53476256219229	0.0
21	0.0010419651462658574	0.0	0.0	61.831774727135375	0.0
22	0.0010419651462658574	0.0	0.0	63.631248534736514	0.0
23	0.0010419651462658574	0.0	0.0	64.95454427049415	0.0
24	0.0010419651462658574	0.0	0.0	65.62192294667743	0.0
25	0.0010419651462658574	0.0	0.0	66.06788402927923	0.0
26	0.0010419651462658574	0.0	0.0	66.41485842298574	0.0
27	0.0010419651462658574	0.0	0.0	66.67795462241789	0.0
28	0.0010419651462658574	0.0	0.0	66.93427804839928	0.0
29	0.0010419651462658574	0.0	0.0	67.14631795566439	0.0
30	0.0010419651462658574	0.0	0.0	67.37555028784287	0.0
31	0.0010419651462658574	0.0	0.0	67.55685222329313	0.0
32	0.0010419651462658574	0.0	0.0	67.72200369897627	0.0
33	0.0010419651462658574	0.0	0.0	67.90017973898773	0.0
34	0.0010419651462658574	0.0	0.0	68.04084503373362	0.0
35	0.0010419651462658574	0.0	0.0	68.17056969444373	0.0
36	0.0010419651462658574	0.0	0.0	68.29247961655683	0.0
37	0.0010419651462658574	0.0	0.0	68.410742660658	0.0
38	0.0010419651462658574	0.0	0.0	68.5560967985621	0.0
39	0.0010419651462658574	0.0	0.0	68.70092995389304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAG	30	2.1560245E-6	45.000004	5
TTAGGGA	30	2.1560245E-6	45.000004	4
GTCTGGT	30	2.1560245E-6	45.000004	3
CTCGGGA	30	2.1560245E-6	45.000004	4
ACTAATC	30	2.1560245E-6	45.000004	10
TAGGGCG	30	2.1560245E-6	45.000004	5
CTGGATA	30	2.1560245E-6	45.000004	5
GACTAAT	30	2.1560245E-6	45.000004	9
CTTCGTG	25	3.8786286E-5	45.0	1
GTGTTAG	20	7.0185907E-4	45.0	1
CATGGGA	25	3.8786286E-5	45.0	4
CTGTCGG	20	7.0185907E-4	45.0	2
TCTTGCG	40	6.7648216E-9	45.0	1
ACACGCG	20	7.0185907E-4	45.0	36
GAGCTAG	20	7.0185907E-4	45.0	1
TGCTTCG	20	7.0185907E-4	45.0	1
AAGAGCA	20	7.0185907E-4	45.0	35
CGTGCGG	20	7.0185907E-4	45.0	2
AACCCGA	20	7.0185907E-4	45.0	24
CGGTTTG	25	3.8786286E-5	45.0	1
>>END_MODULE