##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65769
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02721646976539	33.0	31.0	34.0	30.0	34.0
2	32.22750840061427	34.0	31.0	34.0	30.0	34.0
3	32.28391795526768	33.0	31.0	34.0	30.0	34.0
4	35.9076160501148	37.0	35.0	37.0	35.0	37.0
5	35.92928279280512	37.0	35.0	37.0	35.0	37.0
6	35.89893414830696	37.0	35.0	37.0	35.0	37.0
7	35.95446182852103	37.0	35.0	37.0	35.0	37.0
8	35.76976995240919	37.0	35.0	37.0	35.0	37.0
9	37.6138302239657	39.0	37.0	39.0	35.0	39.0
10	37.133847253265216	39.0	37.0	39.0	33.0	39.0
11	37.15356779029634	39.0	37.0	39.0	34.0	39.0
12	37.24119265915553	39.0	37.0	39.0	34.0	39.0
13	37.17947665313446	39.0	37.0	39.0	33.0	39.0
14	38.28543842843893	40.0	38.0	41.0	33.0	41.0
15	38.329395307819794	40.0	38.0	41.0	34.0	41.0
16	38.42881904848789	40.0	38.0	41.0	34.0	41.0
17	38.40818622755401	40.0	38.0	41.0	34.0	41.0
18	38.00405966336724	39.0	37.0	40.0	34.0	41.0
19	37.54402530067357	38.0	37.0	40.0	34.0	41.0
20	36.97468412169867	38.0	35.0	40.0	33.0	41.0
21	36.827319861941035	38.0	35.0	40.0	33.0	41.0
22	36.86295975307516	38.0	35.0	40.0	33.0	41.0
23	36.81777129042558	38.0	35.0	40.0	33.0	41.0
24	36.70685277258283	38.0	35.0	40.0	33.0	41.0
25	36.594033663276015	38.0	35.0	40.0	33.0	41.0
26	36.527984308716874	38.0	35.0	40.0	33.0	41.0
27	36.43584363453907	38.0	35.0	40.0	32.0	41.0
28	36.31163618117958	38.0	35.0	40.0	32.0	41.0
29	36.194711794310386	38.0	35.0	40.0	31.0	41.0
30	35.96460338457328	38.0	35.0	40.0	31.0	41.0
31	35.427587465219176	37.0	35.0	40.0	29.0	41.0
32	34.90030257416108	37.0	35.0	40.0	25.0	41.0
33	34.38632182335143	37.0	34.0	40.0	21.0	41.0
34	33.84345208228801	37.0	33.0	40.0	18.0	41.0
35	33.48655141480029	37.0	33.0	40.0	15.0	41.0
36	33.2011586005565	37.0	33.0	40.0	13.0	41.0
37	32.836153811066005	37.0	32.0	40.0	10.0	41.0
38	32.66622572944701	37.0	32.0	40.0	10.0	41.0
39	32.58995879517706	37.0	32.0	40.0	10.0	41.0
40	32.299578828931566	36.0	31.0	40.0	10.0	41.0
41	32.09472547856893	36.0	31.0	40.0	10.0	41.0
42	32.05522358557983	36.0	31.0	40.0	10.0	41.0
43	31.956438443643663	36.0	31.0	40.0	10.0	41.0
44	31.844774893946997	36.0	31.0	40.0	9.0	41.0
45	31.78600860587815	36.0	30.0	40.0	9.0	41.0
46	31.48638416275145	35.0	29.0	40.0	8.0	41.0
47	31.232678009396523	35.0	28.0	39.0	8.0	41.0
48	31.24180084842403	35.0	28.0	39.0	8.0	41.0
49	31.394121850719944	35.0	29.0	39.0	8.0	41.0
50	31.31677538049841	35.0	29.0	39.0	8.0	41.0
51	30.389894935303868	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	1.0
14	4.0
15	2.0
16	9.0
17	13.0
18	29.0
19	52.0
20	101.0
21	184.0
22	249.0
23	415.0
24	700.0
25	1338.0
26	2122.0
27	2358.0
28	2251.0
29	2009.0
30	1939.0
31	1971.0
32	2249.0
33	2737.0
34	3787.0
35	5701.0
36	7405.0
37	8278.0
38	11997.0
39	7860.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.65153795861272	4.7377944016177835	36.620596329577765	36.99007131019173
2	42.477458985236204	4.751478660159041	36.39252535389013	16.378537000714623
3	17.444388693761496	5.508674299441986	60.104304459547805	16.942632547248703
4	14.16320759020207	4.839666104091593	60.35366205963296	20.64346424607338
5	11.280390457510377	6.255226626526175	61.566999650291166	20.89738326567228
6	15.432802688196567	7.056515987775396	65.71180951512112	11.79887180890693
7	58.06991135641412	2.993811674193009	34.155909318980065	4.780367650412808
8	56.65283036080828	3.2462102206206573	33.93848165549119	6.16247776307987
9	52.567318949657135	4.7149873040490204	36.325624534355086	6.392069211938756
10	25.86172816980644	16.688713527649803	41.77195943377579	15.677598868767962
11	16.4256716690234	15.411516063799056	46.07945992792957	22.083352339247973
12	14.379114780519696	13.142970092292721	53.40357919384513	19.074335933342457
13	17.49304383524153	14.339582478067175	54.34323161367818	13.824142073013121
14	15.172801775912664	16.675029269108546	52.275388100776965	15.876780854201828
15	11.128343140385288	15.431282215025316	54.35235445270569	19.088020191883714
16	11.395946418525444	14.886952819717497	51.37678845656768	22.340312305189375
17	11.195243959920328	15.43888458088157	49.23900317778892	24.126868281409173
18	12.226124770028433	14.356307682950934	52.55211421794462	20.865453329076008
19	12.76741321899375	16.88941598625492	52.90486399367483	17.438306801076493
20	13.296537882589062	19.55328498228649	50.56181483677721	16.588362298347246
21	12.881448706837567	20.66779181681339	50.355030485487084	16.095728990861957
22	11.085769891590262	19.11690918213748	48.405783879943435	21.391537046328818
23	11.411151150237954	19.96381273852423	45.839225166871934	22.785810944365885
24	12.992443248338883	19.106265869938724	46.138758381608355	21.762532500114034
25	12.432909121318554	20.722528850978424	43.72728793200444	23.11727409569858
26	12.127294013897124	21.692590734236493	45.46975018625796	20.710365065608418
27	13.407532424090377	20.666271343642144	46.61162553786738	19.314570694400096
28	11.502379540513008	19.02872173820493	47.09209505998267	22.376803661299398
29	14.243792668278369	18.200063859873193	45.17173744469279	22.384406027155652
30	19.261354133406318	19.598899177424016	42.094299746080985	19.045446943088688
31	17.39725402545272	26.54898204321185	36.88819960771792	19.16556432361751
32	15.88286274688683	27.28793200443978	34.86292934361173	21.966275905061654
33	18.25023947452447	21.678906475695236	34.57099849473156	25.499855555048732
34	18.84322401131232	23.108151256671075	34.458483480058995	23.590141251957608
35	21.015980173029845	22.66873451017957	31.873679088932477	24.44160622785811
36	22.09399565144673	23.637275920266386	31.122565342334536	23.14616308595235
37	16.5123386397847	29.597530751569888	31.718590825464887	22.171539783180524
38	17.17374446927884	32.82853624047804	29.912268698018824	20.085450592224298
39	22.423938329608173	26.445589867566788	31.190986635040822	19.939485167784216
40	23.266280466481167	26.611321443233134	31.634964801046085	18.48743328923961
41	17.993279508583072	29.883379707765055	29.419635390533532	22.70370539311834
42	17.448950113275252	26.6432513798294	31.152974805759552	24.754823701135795
43	18.00696376712433	22.478675363773206	34.78386473870669	24.730496130395778
44	18.920768143046114	22.045340509966703	34.30795663610516	24.72593471088203
45	22.56990375404826	20.842646231507246	32.63999756724292	23.94745244720157
46	22.916571637093462	22.635284100412047	35.500007602365855	18.948136660128633
47	15.84485091760556	26.374127628517996	38.47861454484636	19.30240690903009
48	15.257948273502715	24.370143988809318	39.153704632881755	21.218203104806214
49	19.921239489729203	18.866031108881085	40.27125241375116	20.941476987638552
50	19.48942510909395	16.950234913104957	37.611944837233345	25.948395140567744
51	17.350119357143942	16.96696011798872	35.16550350469066	30.51741702017668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	415.0
2	592.0
3	3593.5
4	6595.0
5	4047.0
6	1499.0
7	1428.0
8	1357.0
9	1326.0
10	1295.0
11	1222.0
12	1149.0
13	1072.5
14	996.0
15	909.0
16	822.0
17	752.5
18	683.0
19	633.5
20	584.0
21	530.5
22	477.0
23	460.5
24	444.0
25	410.0
26	387.0
27	398.0
28	382.0
29	366.0
30	392.0
31	418.0
32	462.0
33	506.0
34	539.5
35	573.0
36	642.0
37	711.0
38	819.5
39	928.0
40	1161.5
41	1395.0
42	1828.5
43	2262.0
44	2665.0
45	3068.0
46	4177.0
47	5286.0
48	6737.5
49	8189.0
50	8225.0
51	8261.0
52	6821.0
53	5381.0
54	4080.0
55	2779.0
56	2290.0
57	1801.0
58	1605.0
59	1409.0
60	1290.5
61	1172.0
62	1111.0
63	1050.0
64	937.5
65	825.0
66	733.0
67	641.0
68	534.0
69	427.0
70	372.0
71	317.0
72	268.5
73	220.0
74	178.5
75	106.5
76	76.0
77	54.5
78	33.0
79	26.0
80	19.0
81	14.5
82	10.0
83	6.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	65769.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.0368258602077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63282762677385	50.02204686098314
2	11.203156785372908	13.900165731575667
3	3.972941839660793	7.394061031793093
4	1.5955491286978258	3.959312137937326
5	0.862723952844293	2.6760327814015725
6	0.4558711796279503	1.696848059115997
7	0.27940491654616306	1.213337590658213
8	0.15930982083772458	0.7906460490504644
9	0.12744785667017966	0.711581444145418
>10	0.6519448052743806	6.782830816950234
>50	0.03676380480870567	1.4262038346333379
>100	0.019607362564643023	2.6471437911478053
>500	0.0	0.0
>1k	0.002450920320580378	6.779789870607733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4459	6.779789870607733	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	330	0.5017561465127948	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	304	0.46222384406027156	Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	281	0.42725296112150096	Illumina Single End Adapter 1 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	220	0.33450409767519657	Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	217	0.32994267816144385	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	150	0.228070975687634	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.1976615122626161	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109	0.16573157566634739	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.13684258541258038	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.1322811658988277	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	72	0.10947406833006432	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015204731712508933	0.0	0.0	0.4789490489440314	0.0
2	0.0015204731712508933	0.0	0.0	1.765269351822287	0.0
3	0.0015204731712508933	0.0	0.0	2.464687010597698	0.0
4	0.0015204731712508933	0.0	0.0	3.4438717328832733	0.0
5	0.0015204731712508933	0.0	0.0	5.5588499140932655	0.0
6	0.0015204731712508933	0.0	0.0	7.131019173166689	0.0
7	0.0015204731712508933	0.0	0.0	8.566345846827533	0.0
8	0.0015204731712508933	0.0	0.0	10.713253964633793	0.0
9	0.0015204731712508933	0.0	0.0	11.64074259909684	0.0
10	0.0015204731712508933	0.0	0.0	14.05981541455701	0.0
11	0.0015204731712508933	0.0	0.0	19.91667807021545	0.0
12	0.0015204731712508933	0.0	0.0	25.19271997445605	0.0
13	0.0015204731712508933	0.0	0.0	26.85763809697578	0.0
14	0.0015204731712508933	0.0	0.0	27.427815536194863	0.0
15	0.0015204731712508933	0.0	0.0	28.30512855600663	0.0
16	0.0015204731712508933	0.0	0.0	30.395779166476608	0.0
17	0.0015204731712508933	0.0	0.0	32.9349693624656	0.0
18	0.0015204731712508933	0.0	0.0	35.29626419741824	0.0
19	0.0015204731712508933	0.0	0.0	36.66164910520154	0.0
20	0.0015204731712508933	0.0	0.0	37.82024966169472	0.0
21	0.0015204731712508933	0.0	0.0	39.298149584150586	0.0
22	0.0015204731712508933	0.0	0.0	40.33055086742994	0.0
23	0.0015204731712508933	0.0	0.0	41.18809773601545	0.0
24	0.0015204731712508933	0.0	0.0	41.64271921421947	0.0
25	0.0015204731712508933	0.0	0.0	41.94225242895589	0.0
26	0.0015204731712508933	0.0	0.0	42.17184387781477	0.0
27	0.0015204731712508933	0.0	0.0	42.32845261445362	0.0
28	0.0015204731712508933	0.0	0.0	42.49266371694871	0.0
29	0.0015204731712508933	0.0	0.0	42.67664097067007	0.0
30	0.0015204731712508933	0.0	0.0	42.84237254633642	0.0
31	0.0015204731712508933	0.0	0.0	43.00810412200277	0.0
32	0.0015204731712508933	0.0	0.0	43.125180556189086	0.0
33	0.0015204731712508933	0.0	0.0	43.258982195259165	0.0
34	0.0015204731712508933	0.0	0.0	43.41559093189801	0.0
35	0.0015204731712508933	0.0	0.0	43.53722878559808	0.0
36	0.0015204731712508933	0.0	0.0	43.65886663929815	0.0
37	0.0015204731712508933	0.0	0.0	43.79570922471073	0.0
38	0.0015204731712508933	0.0	0.0	43.94319512232207	0.0
39	0.0015204731712508933	0.0	0.0	44.08611960041965	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGC	35	1.1887278E-7	45.000004	4
TGTTGTG	20	6.9848174E-4	45.0	45
TTATGGG	40	6.6484063E-9	45.0	3
CGTTTGG	20	6.9848174E-4	45.0	2
TGTTGCG	20	6.9848174E-4	45.0	1
ATGGGCC	20	6.9848174E-4	45.0	5
TTGTCCT	20	6.9848174E-4	45.0	1
TTCGTGG	25	3.8506878E-5	45.0	2
CGGGCTC	20	6.9848174E-4	45.0	6
TTGGGTA	25	3.8506878E-5	45.0	5
ATTTGGG	20	6.9848174E-4	45.0	3
TGGGCTT	20	6.9848174E-4	45.0	6
TGGGCCG	20	6.9848174E-4	45.0	6
TTTGTTG	20	6.9848174E-4	45.0	1
TTTCGTG	20	6.9848174E-4	45.0	1
CCTGGCC	20	6.9848174E-4	45.0	29
TCGGGCT	20	6.9848174E-4	45.0	5
GGGTACC	30	2.134324E-6	44.999996	7
TACATGG	30	2.134324E-6	44.999996	2
GTGCTTG	30	2.134324E-6	44.999996	1
>>END_MODULE