##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933675.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66605
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932527588018917	33.0	31.0	34.0	30.0	34.0
2	32.14579986487501	34.0	31.0	34.0	30.0	34.0
3	32.24257938593199	33.0	31.0	34.0	30.0	34.0
4	35.8190826514526	37.0	35.0	37.0	35.0	37.0
5	35.89922678477592	37.0	35.0	37.0	35.0	37.0
6	35.8990916597853	37.0	35.0	37.0	35.0	37.0
7	35.87024998123264	37.0	35.0	37.0	35.0	37.0
8	35.722753547031004	37.0	35.0	37.0	35.0	37.0
9	37.58983559792809	39.0	37.0	39.0	35.0	39.0
10	37.16863598828917	39.0	37.0	39.0	33.0	39.0
11	37.1743262517829	39.0	37.0	39.0	34.0	39.0
12	37.208212596651904	39.0	37.0	39.0	34.0	39.0
13	37.18952030628331	39.0	37.0	39.0	34.0	39.0
14	38.357495683507246	40.0	38.0	41.0	34.0	41.0
15	38.3612791832445	40.0	38.0	41.0	34.0	41.0
16	38.507979881390284	40.0	38.0	41.0	34.0	41.0
17	38.5249605885444	40.0	38.0	41.0	34.0	41.0
18	38.167269724495156	39.0	38.0	40.0	34.0	41.0
19	37.77045266871856	38.0	37.0	40.0	34.0	41.0
20	37.13327828241123	38.0	35.0	40.0	34.0	41.0
21	37.03990691389535	38.0	35.0	40.0	33.0	41.0
22	37.02274604008708	38.0	35.0	40.0	33.0	41.0
23	36.93524510171909	38.0	35.0	40.0	33.0	41.0
24	36.843104872006606	38.0	35.0	40.0	33.0	41.0
25	36.733488476841075	38.0	35.0	40.0	33.0	41.0
26	36.706223256512274	38.0	35.0	40.0	33.0	41.0
27	36.631679303355604	38.0	35.0	40.0	33.0	41.0
28	36.556970197432626	38.0	35.0	40.0	33.0	41.0
29	36.38831919525561	38.0	35.0	40.0	32.0	41.0
30	36.07463403648375	38.0	35.0	40.0	31.0	41.0
31	35.573410404624276	37.0	35.0	40.0	30.0	41.0
32	35.21111027700623	38.0	35.0	40.0	27.0	41.0
33	34.63848059454996	38.0	35.0	40.0	22.0	41.0
34	34.10808497860521	38.0	34.0	40.0	18.0	41.0
35	33.78949027850762	37.0	34.0	40.0	16.0	41.0
36	33.50014263193454	37.0	33.0	40.0	15.0	41.0
37	33.22070415133999	37.0	33.0	40.0	12.0	41.0
38	33.02998273402898	37.0	33.0	40.0	10.0	41.0
39	32.95516853089107	37.0	33.0	40.0	10.0	41.0
40	32.679168230613314	37.0	32.0	40.0	10.0	41.0
41	32.53192703250507	36.0	32.0	40.0	10.0	41.0
42	32.4168455821635	37.0	31.0	40.0	10.0	41.0
43	32.35638465580662	37.0	31.0	40.0	10.0	41.0
44	32.22840627580512	36.0	31.0	40.0	10.0	41.0
45	32.1270024772915	36.0	31.0	40.0	10.0	41.0
46	31.799924930560767	35.0	30.0	40.0	10.0	41.0
47	31.664439606636137	35.0	30.0	39.0	10.0	41.0
48	31.615299151715337	35.0	30.0	39.0	9.0	41.0
49	31.677336536296075	35.0	30.0	39.0	8.0	41.0
50	31.523759477516702	35.0	30.0	39.0	8.0	41.0
51	30.703881090008256	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	2.0
13	4.0
14	4.0
15	3.0
16	5.0
17	16.0
18	31.0
19	48.0
20	91.0
21	139.0
22	244.0
23	459.0
24	669.0
25	1287.0
26	1991.0
27	2334.0
28	2103.0
29	2071.0
30	1846.0
31	2002.0
32	2160.0
33	2595.0
34	3813.0
35	5554.0
36	7178.0
37	7882.0
38	12615.0
39	9454.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.86772764807447	4.930560768711058	36.36213497485174	36.839576608362734
2	42.189024847984385	4.7413857818482095	36.578334959837846	16.491254410329557
3	17.836498761354253	5.703775992793334	59.618647248705045	16.841077997147362
4	14.673072592147737	4.957585766834321	59.48202086930411	20.887320771713835
5	11.063733953907365	6.526537046768261	61.565948502364684	20.84378049695969
6	16.046843330080325	7.3282786577584265	64.89602882666466	11.728849185496584
7	58.25538623226485	3.1258914495908714	33.823286540049544	4.795435778094738
8	56.68793634111553	3.2895428271150813	33.71518654755649	6.307334284212897
9	52.70325050671871	4.835973275279634	36.06636138428046	6.394414833721192
10	25.76833571053224	17.33953907364312	41.20861797162375	15.683507244200886
11	16.11290443660386	15.449290593799264	46.110652353426914	22.327152616169958
12	14.405825388484347	13.038060205690263	53.152165753321825	19.403948652503566
13	17.63831544178365	14.371293446437955	54.43134899782298	13.559042113955408
14	15.431273928383755	16.770512724269953	51.987088056452215	15.811125290894076
15	11.129795060430899	15.417761429322125	54.27070039786803	19.181743112378953
16	11.548682531341491	15.01388784625779	51.05022145484573	22.387208167554988
17	11.407551985586668	15.542376698446061	48.81014938818407	24.2399219277832
18	12.422490803993695	14.33075594925306	52.417986637639814	20.82876660911343
19	13.173185196306584	17.031754372794836	52.375947751670296	17.419112679228284
20	13.488476841077999	19.48802642444261	50.3490728924255	16.6744238420539
21	13.185196306583592	20.860295773590572	49.60138127768185	16.353126642143984
22	11.15381728098491	19.120186172209294	48.0549508295173	21.671045717288493
23	11.605735305157271	20.22370692890924	45.42752045642219	22.743037309511298
24	13.21372269349148	19.340890323549285	45.741310712408975	21.70407627055026
25	12.586142181517904	21.06448464829968	43.2790331056227	23.07034006455972
26	12.329404699346897	21.926281810674876	44.93956910141881	20.804744388559417
27	13.434426844831467	20.846783274528942	46.355378725320925	19.36341115531867
28	11.707829742511823	19.00758201336236	46.9304106298326	22.35417761429322
29	14.558967044516177	18.024172359432473	44.789430222956234	22.627430373095116
30	19.588619473012535	19.600630583289544	42.1079498536146	18.702800090083326
31	17.56624878012161	26.577584265445537	36.615869679453496	19.240297274979355
32	16.438705802867652	27.021995345694766	34.57398093236244	21.965317919075144
33	18.45206816305082	21.57495683507244	34.495908715561896	25.47706628631484
34	18.912994519930937	22.851137302004354	34.519930936115905	23.715937241948804
35	20.747691614743637	22.594399819833345	32.11470610314541	24.54320246227761
36	22.165002627430372	23.170933113129642	31.63876585841904	23.025298401020944
37	16.36513775242099	29.61489377674349	31.979581112529086	22.040387358306436
38	17.03325576157946	32.785826889873135	30.07732152240823	20.103595826139177
39	22.457773440432398	26.304331506643646	31.29644921552436	19.941445837399595
40	23.085353952405978	26.42744538698296	31.98408527888297	18.5031153817281
41	17.728398768861197	29.85962014863749	29.754522933713684	22.65745814878763
42	17.597777944598754	26.66466481495383	31.177839501538923	24.55971773890849
43	17.81247654080024	22.279108175061932	35.13550033781247	24.77291494632535
44	19.147211170332557	21.914270700397868	34.537947601531414	24.400570527738157
45	22.72802342166504	20.490954132572632	32.806846332857894	23.974176112904434
46	23.148412281360258	22.298626229262066	35.8651752871406	18.68778620223707
47	15.929735004879515	26.446963441183097	38.63073342842129	18.9925681255161
48	15.407251707829742	24.293971924029726	39.345394489903164	20.95338187823737
49	20.022520831769384	18.906988964792433	40.44741385781848	20.623076345619697
50	19.433976428196083	16.965693266271302	37.597777944598754	26.002552360933862
51	17.39208768110502	16.838075219578112	35.462803092860895	30.307034006455975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	408.0
2	602.0
3	3667.5
4	6733.0
5	4173.5
6	1614.0
7	1477.0
8	1340.0
9	1293.0
10	1246.0
11	1192.5
12	1139.0
13	1067.0
14	995.0
15	897.5
16	800.0
17	726.5
18	653.0
19	581.5
20	510.0
21	477.5
22	445.0
23	442.5
24	440.0
25	417.0
26	378.5
27	363.0
28	357.5
29	352.0
30	399.0
31	446.0
32	465.0
33	484.0
34	556.0
35	628.0
36	683.5
37	739.0
38	849.5
39	960.0
40	1221.0
41	1482.0
42	1953.0
43	2424.0
44	2822.0
45	3220.0
46	4203.0
47	5186.0
48	6732.0
49	8278.0
50	8245.0
51	8212.0
52	6806.0
53	5400.0
54	4139.5
55	2879.0
56	2384.0
57	1889.0
58	1673.5
59	1458.0
60	1302.5
61	1147.0
62	1137.5
63	1128.0
64	1007.0
65	886.0
66	764.5
67	643.0
68	537.5
69	432.0
70	373.5
71	315.0
72	274.5
73	234.0
74	190.5
75	115.5
76	84.0
77	56.0
78	28.0
79	25.0
80	22.0
81	15.0
82	8.0
83	6.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	66605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.87673598078223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29505253196807	49.68395766083628
2	11.341081697522625	14.03498235868178
3	4.137044136558853	7.679603633360858
4	1.737315895469876	4.299977479168231
5	0.8079974765243976	2.499812326401922
6	0.43918181156431224	1.6305082201035956
7	0.24749472253900467	1.071991592222806
8	0.22080411520636695	1.093011035207567
9	0.1164681047242375	0.6485999549583366
>10	0.5968990367117172	6.292320396366639
>50	0.038822701574745835	1.568951279933939
>100	0.019411350787372918	2.6904887020493957
>500	0.0	0.0
>1k	0.0024264188484216147	6.805795360708656
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4533	6.805795360708656	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	346	0.5194805194805194	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	317	0.4759402447263719	Illumina Single End Adapter 1 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	297	0.44591246903385634	Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	218	0.32730275504841977	Illumina Single End Adapter 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	207	0.31078747841753623	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	175	0.2627430373095113	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121	0.18166804293971925	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.16665415509346143	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.14413332332407477	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	88	0.13212221304706853	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.12911943547781699	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	76	0.1141055476315592	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	74	0.11110277006230763	Illumina Single End Adapter 1 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	67	0.10059304856992718	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015013887846257788	0.0	0.0	0.5059680204188874	0.0
2	0.0015013887846257788	0.0	0.0	1.7130846032580136	0.0
3	0.0015013887846257788	0.0	0.0	2.3797012236318595	0.0
4	0.0015013887846257788	0.0	0.0	3.412656707454395	0.0
5	0.0015013887846257788	0.0	0.0	5.519105172284363	0.0
6	0.0015013887846257788	0.0	0.0	7.053524510171909	0.0
7	0.0015013887846257788	0.0	0.0	8.463328578935515	0.0
8	0.0015013887846257788	0.0	0.0	10.62082426244276	0.0
9	0.0015013887846257788	0.0	0.0	11.494632535094963	0.0
10	0.0015013887846257788	0.0	0.0	13.952405975527363	0.0
11	0.0015013887846257788	0.0	0.0	19.777794459875384	0.0
12	0.0015013887846257788	0.0	0.0	25.073192703250506	0.0
13	0.0015013887846257788	0.0	0.0	26.723218977554236	0.0
14	0.0015013887846257788	0.0	0.0	27.25921477366564	0.0
15	0.0015013887846257788	0.0	0.0	28.101493881840703	0.0
16	0.0015013887846257788	0.0	0.0	30.09383679903911	0.0
17	0.0015013887846257788	0.0	0.0	32.63718940019518	0.0
18	0.0015013887846257788	0.0	0.0	34.89978229862623	0.0
19	0.0015013887846257788	0.0	0.0	36.20899331881991	0.0
20	0.0015013887846257788	0.0	0.0	37.369566849335634	0.0
21	0.0015013887846257788	0.0	0.0	38.686284813452446	0.0
22	0.0015013887846257788	0.0	0.0	39.72674724119811	0.0
23	0.0015013887846257788	0.0	0.0	40.59154718114256	0.0
24	0.0030027775692515577	0.0	0.0	41.04346520531492	0.0
25	0.0030027775692515577	0.0	0.0	41.339238795886196	0.0
26	0.0030027775692515577	0.0	0.0	41.541926281810674	0.0
27	0.0030027775692515577	0.0	0.0	41.71758876961189	0.0
28	0.0030027775692515577	0.0	0.0	41.90976653404399	0.0
29	0.0030027775692515577	0.0	0.0	42.11095263118384	0.0
30	0.0030027775692515577	0.0	0.0	42.24307484423091	0.0
31	0.0030027775692515577	0.0	0.0	42.39021094512424	0.0
32	0.0030027775692515577	0.0	0.0	42.51482621424818	0.0
33	0.0030027775692515577	0.0	0.0	42.6499512048645	0.0
34	0.0030027775692515577	0.0	0.0	42.77907064034232	0.0
35	0.0030027775692515577	0.0	0.0	42.900683131897004	0.0
36	0.0030027775692515577	0.0	0.0	43.03730951129795	0.0
37	0.0030027775692515577	0.0	0.0	43.15441783649876	0.0
38	0.0030027775692515577	0.0	0.0	43.28353727197658	0.0
39	0.0030027775692515577	0.0	0.0	43.41415809623902	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCTG	20	6.9854734E-4	45.0	1
AATTCGA	20	6.9854734E-4	45.0	13
TGATTGG	25	3.8512288E-5	45.0	2
AGGGCCT	35	1.189037E-7	45.0	6
AGGGTCT	20	6.9854734E-4	45.0	6
GTATTGG	20	6.9854734E-4	45.0	2
CTAGGGT	20	6.9854734E-4	45.0	4
TCCGGGC	20	6.9854734E-4	45.0	4
GTCTTTG	20	6.9854734E-4	45.0	1
TCGTTTG	25	3.8512288E-5	45.0	1
CGAGCTC	20	6.9854734E-4	45.0	17
GAATTCG	20	6.9854734E-4	45.0	12
GGGACAT	20	6.9854734E-4	45.0	7
CGGTTTT	20	6.9854734E-4	45.0	1
TGGGACA	45	3.7289283E-10	45.0	6
TCGAGCT	20	6.9854734E-4	45.0	16
TGCGGGC	20	6.9854734E-4	45.0	4
TTACGGG	20	6.9854734E-4	45.0	3
TATTGGG	30	2.1347423E-6	44.999996	3
CGTTTTT	1490	0.0	44.395973	1
>>END_MODULE