FastQCFastQC Report
Sat 14 Jan 2017
SRR2933674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121743
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85907.055847153429766No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC10180.8361877068907453No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC8270.67929983654091No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT7150.5873027607336767Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC5480.4501285494853914No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC5400.4435573297848747No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC4770.391808974643306No Hit
TCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG2310.18974396885241862No Hit
GTGTTTGTGTGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC2290.18810116392728946No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT1850.15195945557444782No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG1700.13963841863597906No Hit
GGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT1560.1281387841600749No Hit
TGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG1510.124031771847252No Hit
GCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG1490.12238896692212282No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1410.11581774722160616No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG1390.11417494229647701No Hit
GTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC1310.10760372259596034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.10596091767083118No Hit
GTGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC1270.10431811274570202No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC1270.10431811274570202No Hit
GTTCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC1270.10431811274570202No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.10185390535800826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTG351.1999873E-745.0000045
TGTATGG351.1999873E-745.0000042
CGTTCGG253.870213E-545.045
TTGTCCG253.870213E-545.01
GCCTTTG207.008425E-445.01
GGTTGAT207.008425E-445.015
GCGATTC253.870213E-545.09
GTGTATG207.008425E-445.01
TGTCCGG253.870213E-545.02
TCAGGGC207.008425E-445.04
CCCGGGC253.870213E-545.04
CGGGGAA207.008425E-445.06
GTCCGGG253.870213E-545.03
TACGGGA253.870213E-545.04
CCATGTA207.008425E-445.03
TAGCAGG253.870213E-545.02
AGGGAGC207.008425E-445.06
GCTACCT207.008425E-445.013
TTCCTTG253.870213E-545.01
GACATGG207.008425E-445.02
Produced by FastQC (version 0.11.5)