##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933674.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	121743
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95341005232334	33.0	31.0	34.0	30.0	34.0
2	32.150834134200736	33.0	31.0	34.0	30.0	34.0
3	32.21770450867812	33.0	31.0	34.0	30.0	34.0
4	35.885085795487214	37.0	35.0	37.0	35.0	37.0
5	35.917670831177155	37.0	35.0	37.0	35.0	37.0
6	35.871532654854896	37.0	35.0	37.0	35.0	37.0
7	35.94161471296091	37.0	35.0	37.0	35.0	37.0
8	35.74585807808252	37.0	35.0	37.0	35.0	37.0
9	37.55346919330064	39.0	37.0	39.0	35.0	39.0
10	37.097837247316065	39.0	37.0	39.0	33.0	39.0
11	37.13328897760036	39.0	37.0	39.0	34.0	39.0
12	37.23986594711811	39.0	37.0	39.0	34.0	39.0
13	37.188618647478705	39.0	37.0	39.0	33.0	39.0
14	38.21827127637729	40.0	38.0	41.0	33.0	41.0
15	38.26008887574645	40.0	38.0	41.0	33.0	41.0
16	38.3401756158465	40.0	38.0	41.0	34.0	41.0
17	38.36422627995039	40.0	38.0	41.0	34.0	41.0
18	37.99049637350813	39.0	38.0	40.0	34.0	41.0
19	37.56223355757621	38.0	37.0	40.0	34.0	41.0
20	36.96388293372104	38.0	35.0	40.0	33.0	41.0
21	36.847161643790606	38.0	35.0	40.0	33.0	41.0
22	36.861445832614606	38.0	35.0	40.0	33.0	41.0
23	36.81832220332996	38.0	35.0	40.0	33.0	41.0
24	36.714316223520036	38.0	35.0	40.0	33.0	41.0
25	36.623748387997665	38.0	35.0	40.0	33.0	41.0
26	36.57307607008206	38.0	35.0	40.0	33.0	41.0
27	36.52095808383233	38.0	35.0	40.0	32.0	41.0
28	36.411210500809084	38.0	35.0	40.0	32.0	41.0
29	36.34609792760158	38.0	35.0	40.0	32.0	41.0
30	36.1576764167139	38.0	35.0	40.0	31.0	41.0
31	35.7109731154974	38.0	35.0	40.0	30.0	41.0
32	35.10395669566218	38.0	35.0	40.0	26.0	41.0
33	34.545337308921255	38.0	34.0	40.0	21.0	41.0
34	34.16192306744536	38.0	34.0	40.0	18.0	41.0
35	33.820835694865416	38.0	34.0	40.0	16.0	41.0
36	33.45044889644579	37.0	33.0	40.0	15.0	41.0
37	33.0791421272681	37.0	33.0	40.0	12.0	41.0
38	32.8826790862719	37.0	32.0	40.0	10.0	41.0
39	32.820014292402846	37.0	32.0	40.0	10.0	41.0
40	32.88859318400237	37.0	33.0	40.0	10.0	41.0
41	32.67609636693691	37.0	32.0	40.0	10.0	41.0
42	32.246387882670874	36.0	31.0	40.0	10.0	41.0
43	31.903780915535183	36.0	30.0	39.0	10.0	41.0
44	31.995589068776027	36.0	30.0	39.0	10.0	41.0
45	32.15187731532819	37.0	31.0	40.0	10.0	41.0
46	31.946682766154932	36.0	30.0	40.0	10.0	41.0
47	31.62374017397304	36.0	30.0	39.0	9.0	41.0
48	31.524465472347487	35.0	29.0	39.0	8.0	41.0
49	31.619863154349737	36.0	30.0	39.0	8.0	41.0
50	31.608659224760355	36.0	29.0	39.0	8.0	41.0
51	30.583828228317028	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	2.0
14	2.0
15	2.0
16	6.0
17	16.0
18	41.0
19	80.0
20	150.0
21	264.0
22	390.0
23	766.0
24	1387.0
25	2553.0
26	3710.0
27	4168.0
28	3767.0
29	3371.0
30	3475.0
31	3461.0
32	4170.0
33	5024.0
34	7011.0
35	10245.0
36	13804.0
37	16341.0
38	24968.0
39	12560.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.441068480323306	4.066763592157249	37.49620101361064	36.99596691390881
2	41.926845896684	4.438037505236441	36.825115201695375	16.810001396384187
3	16.678577002373853	5.130479781178384	60.58336002891337	17.607583187534395
4	13.803668383397813	4.341933417116384	61.13205687390651	20.722341325579293
5	11.127539160362403	5.632356685805344	62.22945056389278	21.010653589939462
6	15.11873372596371	6.822568854061424	66.10154177242224	11.95715564755263
7	56.62420015935208	2.139753414980738	35.822182794904016	5.413863630763165
8	53.64168781778008	2.7229491634015917	35.74907797573577	7.88628504308256
9	48.43646041250832	4.660637572591442	38.6149511676236	8.28795084727664
10	23.662962141560502	16.36644406659931	44.18159565642378	15.78899813541641
11	15.53436337202139	14.320330532350937	47.7481251488792	22.39718094674848
12	14.149478820137503	11.787125337801763	54.3809500340882	19.68244580797253
13	18.148887410364456	13.404466786591426	55.36416878177801	13.08247702126611
14	14.929811159573855	16.253090526765398	52.54922254256918	16.26787577109156
15	9.887221441889883	14.725281946395274	55.65083824121305	19.736658370501793
16	8.995178367544746	14.973345490089779	52.10320100539661	23.92827513696886
17	8.731508177061514	15.064521163434447	50.16715540113189	26.036815258372144
18	10.06053736149101	13.936735582333275	54.884469743640295	21.118257312535423
19	10.658518354238026	16.799323164370847	54.994537673623945	17.54762080776718
20	10.794871163023746	20.12682454021997	52.310194425962884	16.768109870793392
21	9.884757234502189	22.033299655832366	51.89867179221803	16.18327131744741
22	7.749932234296838	20.08821862447944	48.92437347527168	23.237475665952047
23	8.495765670305481	20.66812876305003	46.54230633383439	24.293799232810102
24	10.872082994504817	19.204389574759944	46.47659413682923	23.446933293906014
25	8.84732592428312	22.1252967316396	44.71715006201589	24.31022728206139
26	8.713437322885094	22.342968384219215	48.02493777876346	20.91865651413223
27	12.379356513310826	20.65745053103669	48.99994250182762	17.963250453824863
28	8.842397509507734	17.868789170629935	50.4127547374387	22.87605858242363
29	12.140328396704533	16.53401016896249	48.52517187846529	22.80048955586769
30	21.009010785014333	18.184207716254733	44.00827973682265	16.798501761908284
31	18.7879385262397	23.457611525919354	39.64170424582933	18.112745702011615
32	16.76975267571852	24.181267095438752	38.76608922073548	20.282891008107242
33	22.172938074468348	17.74065038646986	37.78533468043337	22.30107685862842
34	21.206147376029833	15.568040872986538	40.257756092752764	22.968055658230863
35	21.862447943618935	16.484726021208612	40.314432862669726	21.33839317250273
36	27.115316691719443	16.451869922706027	42.58725347658592	13.845559908988609
37	24.555005215905638	19.54527159672425	42.32522609102782	13.574497096342295
38	22.14829600059141	23.75249500998004	37.8797959636283	16.21941302580025
39	28.17081885611493	19.74651520005257	35.988106092342065	16.094559851490434
40	33.65039468388326	14.758138044897859	36.10228103463854	15.489186236580338
41	31.134438941047943	14.882169816745112	36.531053120097255	17.45233812210969
42	26.75307820572846	14.220119431918057	44.25223626820433	14.774566094149149
43	21.34332158727812	15.086699029923691	47.43845642049235	16.13152296230584
44	20.839801877726032	16.23009125781359	40.963340808095744	21.966766056364637
45	27.165422241935882	13.754384235643938	36.53762433979777	22.54256918262241
46	30.64406167089689	14.193834553115991	39.95958699884182	15.202516777145297
47	21.5872781186598	18.059354541944916	45.205884527241814	15.14748281215347
48	19.489416229269853	16.925819143605793	45.92379027952326	17.660974347601094
49	23.61121378641893	11.94072759830134	46.39281108564764	18.055247529632094
50	22.89741504645031	10.217425231840844	40.853272878112094	26.03188684359676
51	20.3789950962273	10.223996451541362	37.11589167344324	32.2811167787881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	221.0
1	366.0
2	511.0
3	6732.5
4	12954.0
5	7834.0
6	2714.0
7	2537.5
8	2361.0
9	2293.5
10	2226.0
11	2058.5
12	1891.0
13	1752.5
14	1614.0
15	1480.5
16	1347.0
17	1232.5
18	1118.0
19	1039.0
20	960.0
21	864.0
22	768.0
23	675.0
24	582.0
25	575.0
26	520.5
27	473.0
28	469.5
29	466.0
30	505.5
31	545.0
32	590.0
33	635.0
34	687.5
35	740.0
36	844.0
37	948.0
38	1213.0
39	1478.0
40	2086.5
41	2695.0
42	3782.0
43	4869.0
44	5977.5
45	7086.0
46	9989.0
47	12892.0
48	15678.5
49	18465.0
50	17980.5
51	17496.0
52	14055.0
53	10614.0
54	7791.0
55	4968.0
56	3554.0
57	2140.0
58	1718.0
59	1296.0
60	1117.0
61	938.0
62	916.5
63	895.0
64	864.0
65	833.0
66	646.5
67	460.0
68	404.0
69	348.0
70	272.0
71	196.0
72	188.0
73	180.0
74	151.5
75	94.5
76	66.0
77	53.5
78	41.0
79	25.5
80	10.0
81	9.0
82	8.0
83	5.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	121743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.191797475008826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17208084444808	38.734054524695466
2	11.350953277145896	11.394494960695893
3	4.603551264217331	6.931815381582513
4	2.1700351853367152	4.356718661442547
5	1.2633990671794453	3.1706135054992894
6	0.7675312985844039	2.311426529656736
7	0.5318713689550774	1.8686906023344256
8	0.3698551673349153	1.4850956523167658
9	0.25693478438752965	1.1606416796037555
>10	1.3648637591031831	12.172363092744552
>50	0.10146469192373782	3.421141256581487
>100	0.03764012764912855	2.940620815981206
>500	0.006546109156370183	2.160288476544853
>1k	0.0016365272890925457	0.8361877068907453
>5k	0.0016365272890925457	7.055847153429766
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8590	7.055847153429766	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCC	1018	0.8361877068907453	No Hit
CCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	827	0.67929983654091	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	715	0.5873027607336767	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	548	0.4501285494853914	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	540	0.4435573297848747	No Hit
GCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGC	477	0.391808974643306	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	231	0.18974396885241862	No Hit
GTGTTTGTGTGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGC	229	0.18810116392728946	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	185	0.15195945557444782	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCG	170	0.13963841863597906	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCT	156	0.1281387841600749	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	151	0.124031771847252	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	149	0.12238896692212282	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	141	0.11581774722160616	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTG	139	0.11417494229647701	No Hit
GTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	131	0.10760372259596034	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129	0.10596091767083118	No Hit
GTGCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	127	0.10431811274570202	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTC	127	0.10431811274570202	No Hit
GTTCCTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTC	127	0.10431811274570202	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124	0.10185390535800826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.214024625645828E-4	0.0	0.0	0.6513721528137142	0.0
2	8.214024625645828E-4	0.0	0.0	2.349211042934707	0.0
3	8.214024625645828E-4	0.0	0.0	3.3357154004747707	0.0
4	8.214024625645828E-4	0.0	0.0	4.638459706102199	0.0
5	8.214024625645828E-4	0.0	0.0	7.6538281461767825	0.0
6	8.214024625645828E-4	0.0	0.0	9.732797778927742	0.0
7	8.214024625645828E-4	0.0	0.0	11.6934854570694	0.0
8	8.214024625645828E-4	0.0	0.0	14.836992681304059	0.0
9	8.214024625645828E-4	0.0	0.0	16.298267662206452	0.0
10	8.214024625645828E-4	0.0	0.0	19.954330023081408	0.0
11	8.214024625645828E-4	0.0	0.0	27.697691037677732	0.0
12	8.214024625645828E-4	0.0	0.0	34.56297281979251	0.0
13	8.214024625645828E-4	0.0	0.0	36.78404507856715	0.0
14	8.214024625645828E-4	0.0	0.0	37.630089615008664	0.0
15	8.214024625645828E-4	0.0	0.0	38.87122873594375	0.0
16	8.214024625645828E-4	0.0	0.0	41.755172782008	0.0
17	8.214024625645828E-4	0.0	0.0	45.465447705412224	0.0
18	8.214024625645828E-4	0.0	0.0	48.663988894638706	0.0
19	8.214024625645828E-4	0.0	0.0	50.406183517738185	0.0
20	8.214024625645828E-4	0.0	0.0	51.92906368333292	0.0
21	8.214024625645828E-4	0.0	0.0	53.63511659807956	0.0
22	8.214024625645828E-4	0.0	0.0	55.08324913958092	0.0
23	8.214024625645828E-4	0.0	0.0	56.1412155113641	0.0
24	8.214024625645828E-4	0.0	0.0	56.70141199083315	0.0
25	8.214024625645828E-4	0.0	0.0	57.02668736600872	0.0
26	8.214024625645828E-4	0.0	0.0	57.28132212940374	0.0
27	8.214024625645828E-4	0.0	0.0	57.49160115982028	0.0
28	8.214024625645828E-4	0.0	0.0	57.70763000747476	0.0
29	8.214024625645828E-4	0.0	0.0	57.903123793565136	0.0
30	8.214024625645828E-4	0.0	0.0	58.07479690824113	0.0
31	8.214024625645828E-4	0.0	0.0	58.199650082550946	0.0
32	8.214024625645828E-4	0.0	0.0	58.335181488874106	0.0
33	8.214024625645828E-4	0.0	0.0	58.459213260721356	0.0
34	8.214024625645828E-4	0.0	0.0	58.60624430152041	0.0
35	8.214024625645828E-4	0.0	0.0	58.70563399949073	0.0
36	8.214024625645828E-4	0.0	0.0	58.810773514698994	0.0
37	8.214024625645828E-4	0.0	0.0	58.91427022498213	0.0
38	8.214024625645828E-4	0.0	0.0	59.02762376481605	0.0
39	8.214024625645828E-4	0.0	0.0	59.123727852936106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGGTG	35	1.1999873E-7	45.000004	5
TGTATGG	35	1.1999873E-7	45.000004	2
CGTTCGG	25	3.870213E-5	45.0	45
TTGTCCG	25	3.870213E-5	45.0	1
GCCTTTG	20	7.008425E-4	45.0	1
GGTTGAT	20	7.008425E-4	45.0	15
GCGATTC	25	3.870213E-5	45.0	9
GTGTATG	20	7.008425E-4	45.0	1
TGTCCGG	25	3.870213E-5	45.0	2
TCAGGGC	20	7.008425E-4	45.0	4
CCCGGGC	25	3.870213E-5	45.0	4
CGGGGAA	20	7.008425E-4	45.0	6
GTCCGGG	25	3.870213E-5	45.0	3
TACGGGA	25	3.870213E-5	45.0	4
CCATGTA	20	7.008425E-4	45.0	3
TAGCAGG	25	3.870213E-5	45.0	2
AGGGAGC	20	7.008425E-4	45.0	6
GCTACCT	20	7.008425E-4	45.0	13
TTCCTTG	25	3.870213E-5	45.0	1
GACATGG	20	7.008425E-4	45.0	2
>>END_MODULE