##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933672.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424150
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2566709890369	33.0	31.0	34.0	30.0	34.0
2	32.427464340445596	34.0	31.0	34.0	30.0	34.0
3	32.54975362489685	34.0	31.0	34.0	31.0	34.0
4	36.04942355298833	37.0	35.0	37.0	35.0	37.0
5	36.07727219144171	37.0	35.0	37.0	35.0	37.0
6	36.05013792290463	37.0	35.0	37.0	35.0	37.0
7	36.1366026169987	37.0	35.0	37.0	35.0	37.0
8	36.014391135211596	37.0	35.0	37.0	35.0	37.0
9	37.84935989626312	39.0	38.0	39.0	35.0	39.0
10	37.08890251090416	39.0	37.0	39.0	33.0	39.0
11	37.08252976541318	39.0	37.0	39.0	33.0	39.0
12	37.19322645290581	39.0	37.0	39.0	34.0	39.0
13	37.23926676883178	39.0	37.0	39.0	34.0	39.0
14	38.45376399858541	40.0	38.0	41.0	34.0	41.0
15	38.48725922433101	40.0	38.0	41.0	34.0	41.0
16	38.530045974301544	40.0	38.0	41.0	34.0	41.0
17	38.477194388777555	40.0	38.0	41.0	34.0	41.0
18	38.18739832606389	39.0	38.0	41.0	34.0	41.0
19	37.891361546622655	39.0	37.0	41.0	34.0	41.0
20	37.51674171873158	39.0	35.0	41.0	34.0	41.0
21	37.39418366144053	39.0	35.0	41.0	33.0	41.0
22	37.40573146292585	39.0	35.0	41.0	33.0	41.0
23	37.326372745490985	39.0	35.0	40.0	33.0	41.0
24	37.2188376753507	39.0	35.0	41.0	33.0	41.0
25	37.13716373924319	39.0	35.0	40.0	33.0	41.0
26	37.07036190027113	39.0	35.0	40.0	33.0	41.0
27	36.98149239655782	39.0	35.0	40.0	33.0	41.0
28	36.8350182718378	38.0	35.0	40.0	32.0	41.0
29	36.6632842154898	38.0	35.0	40.0	32.0	41.0
30	36.48612519155959	38.0	35.0	40.0	31.0	41.0
31	36.15725333018979	38.0	35.0	40.0	30.0	41.0
32	35.67934457149593	38.0	35.0	40.0	27.0	41.0
33	35.16728751620889	39.0	35.0	40.0	23.0	41.0
34	34.71151479429447	39.0	35.0	41.0	18.0	41.0
35	34.3656088647884	39.0	34.0	41.0	16.0	41.0
36	34.21349051043263	38.0	34.0	41.0	15.0	41.0
37	34.108843569491924	38.0	34.0	41.0	15.0	41.0
38	34.05486738182247	38.0	34.0	41.0	14.0	41.0
39	33.984953436284336	38.0	34.0	41.0	12.0	41.0
40	33.85254273252387	38.0	33.0	40.0	12.0	41.0
41	33.70633266533066	38.0	33.0	40.0	10.0	41.0
42	33.624687021101025	38.0	33.0	40.0	10.0	41.0
43	33.52172344689379	38.0	33.0	40.0	10.0	41.0
44	33.30591771778852	38.0	33.0	40.0	10.0	41.0
45	33.275468584227276	38.0	33.0	40.0	10.0	41.0
46	33.09007426617942	38.0	33.0	40.0	10.0	41.0
47	32.95228810562301	38.0	32.0	40.0	10.0	41.0
48	32.88517977130732	37.0	32.0	40.0	10.0	41.0
49	32.88037015206884	37.0	32.0	40.0	10.0	41.0
50	32.783795826948015	37.0	32.0	40.0	10.0	41.0
51	31.81379700577626	36.0	30.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	1.0
13	5.0
14	21.0
15	19.0
16	39.0
17	67.0
18	154.0
19	286.0
20	550.0
21	901.0
22	1540.0
23	2787.0
24	5081.0
25	9015.0
26	13141.0
27	13253.0
28	11035.0
29	9389.0
30	8853.0
31	9338.0
32	11035.0
33	13773.0
34	20874.0
35	27386.0
36	35311.0
37	49276.0
38	87326.0
39	93663.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.845455617116585	4.752563951432276	41.43864198986208	30.963338441589062
2	34.96475303548273	4.662265707886361	44.727337026995166	15.64564422963574
3	18.65283508192856	4.704467759047507	59.859955204526706	16.78274195449723
4	14.635859955204527	4.865495697276907	60.19521395732642	20.303430390192148
5	13.514322763173405	5.605092538017211	61.454438288341386	19.426146410467997
6	17.5730284097607	6.641518330779206	62.85276435223388	12.932688907226217
7	70.76765295296475	1.9283272427207356	24.052339974065777	3.2516798302487326
8	71.952846870211	2.0761523046092187	22.463279500176824	3.5077213250029473
9	66.9194860308853	4.6837203819403515	24.104208416833668	4.292585170340681
10	29.16963338441589	20.83838264764824	33.33325474478369	16.658729223152186
11	20.92042909348108	22.400801603206414	36.94754214310974	19.731227160202756
12	17.07226217140163	20.447483201697516	43.473063774608036	19.007190852292823
13	18.897324059884475	20.473417423081457	45.30307674171873	15.326181775315337
14	18.612283390310033	21.626782977720147	44.187433690911234	15.57349994105859
15	15.92384769539078	21.896498880113167	45.102675940115525	17.076977484380524
16	16.644111752917603	20.836496522456677	43.07037604621007	19.449015678415655
17	17.01002004008016	21.05151479429447	41.4492514440646	20.489213721560766
18	18.23765177413651	19.44265000589414	43.140398443946715	19.179299776022635
19	17.85665448544147	22.85913002475539	42.09006247789697	17.194153011906167
20	19.04656371566663	23.03524696451727	40.791465283508195	17.126724036307913
21	18.27773193445715	24.57031710479783	40.89897441942709	16.252976541317928
22	17.23541200047153	22.4899210185076	40.1563126252505	20.118354355770364
23	16.629494282683012	23.763055522810326	38.93127431333255	20.676175881174114
24	16.429093481079807	23.7293410350112	39.82647648237651	20.015089001532477
25	16.317576329128848	25.81209477779088	37.91182364729459	19.95850524578569
26	15.69751267240363	26.67994813155723	38.73794648119769	18.884592714841446
27	16.07568077331133	25.386066250147355	39.8903689732406	18.647884003300717
28	15.40209831427561	24.113639042791466	40.203937286337386	20.280325356595544
29	16.649298597194388	23.331368619592123	38.762230343039015	21.257102440174467
30	19.131203583637866	24.14381704585642	37.188023105033594	19.53695626547212
31	18.162678297772015	26.700695508664385	34.94188376753507	20.19474242602853
32	16.723564776612047	26.497701284922787	34.71932099493104	22.059412943534127
33	19.50017682423671	24.258399151243665	32.57220322998939	23.669220794530236
34	18.48214075209242	25.414829659318638	32.59625132618177	23.506778262407167
35	17.953554167157844	26.778262407167276	31.271248379111167	23.996935046563717
36	18.805375456795943	27.6732288105623	31.008369680537545	22.51302605210421
37	17.790168572438997	26.461393374985263	33.36814806082754	22.380289991748203
38	18.77991276670989	25.622303430390193	32.50618884828481	23.091594954615115
39	22.045031238948486	24.033714487799127	30.51679830248733	23.40445597076506
40	21.91088058469881	24.753035482730166	30.550512790286454	22.78557114228457
41	18.33195803371449	25.700106094542026	32.28126841919132	23.68666745255216
42	18.06955086643876	26.00377225038312	35.12695980195685	20.799717081221267
43	18.445597076505955	24.266650948956737	35.875279971708125	21.41247200282919
44	19.847223859483673	24.49557939408228	31.991512436637983	23.665684309796063
45	21.660497465519274	23.671106919721797	30.164092891665682	24.504302723093247
46	21.54827301662148	24.40787457267476	31.783567134268537	22.260285276435223
47	18.316161735235177	26.028527643522338	34.66202994223742	20.993280679005068
48	18.147353530590593	25.125780973712132	35.59023930213368	21.136626193563597
49	20.86384533773429	23.245549923376164	34.59530826358599	21.295296475303548
50	20.196157019922197	22.407403041376874	32.85323588353177	24.543204055169163
51	19.314629258517037	22.085111399269127	31.59731227160203	27.00294707061181
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526.0
1	1028.0
2	1530.0
3	10033.0
4	18536.0
5	12405.5
6	6275.0
7	6071.0
8	5867.0
9	5842.5
10	5818.0
11	5810.0
12	5802.0
13	5652.0
14	5502.0
15	5257.0
16	5012.0
17	4868.0
18	4724.0
19	4578.5
20	4433.0
21	4290.5
22	4148.0
23	4166.0
24	4184.0
25	4164.5
26	4292.0
27	4439.0
28	4788.0
29	5137.0
30	5607.0
31	6077.0
32	7209.5
33	8342.0
34	9434.0
35	10526.0
36	11741.0
37	12956.0
38	14372.0
39	15788.0
40	17140.0
41	18492.0
42	20738.0
43	22984.0
44	25031.0
45	27078.0
46	30938.5
47	34799.0
48	38318.5
49	41838.0
50	40179.0
51	38520.0
52	33357.5
53	28195.0
54	23804.0
55	19413.0
56	16565.0
57	13717.0
58	12381.5
59	11046.0
60	9698.0
61	8350.0
62	7174.0
63	5998.0
64	5339.5
65	4681.0
66	3926.0
67	3171.0
68	2559.0
69	1947.0
70	1692.0
71	1437.0
72	1246.0
73	1055.0
74	928.0
75	646.0
76	491.0
77	341.0
78	191.0
79	133.0
80	75.0
81	64.0
82	53.0
83	41.5
84	30.0
85	23.5
86	17.0
87	9.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	424150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.80931644463817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11955804645484	36.665342798707805
2	9.811707301487512	9.578054530809418
3	4.62326761500673	6.769755960873332
4	2.738163395900251	5.345915346704817
5	1.773295683647551	4.3276675086532155
6	1.2822933660236113	3.755271760626401
7	0.9407993896930417	3.21438425857151
8	0.686824140572898	2.6818733455231385
9	0.5705397670629929	2.5062890431344997
>10	2.3721545561856923	18.017434056605317
>50	0.05571800515453844	1.853555965850214
>100	0.022771706454432276	1.785921747140327
>500	0.0019380175705899813	0.6380318953634272
>1k	4.845043926474953E-4	0.3845218168498638
>5k	0.0	0.0
>10k+	4.845043926474953E-4	2.4759799645867617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10470	2.4684663444536135	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	1626	0.3833549451844866	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	795	0.18743369091123424	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	691	0.16291406342095957	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	660	0.15560532830366616	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	552	0.1301426382176117	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3576564894494873E-4	0.0	0.0	0.1805964870918307	0.0
2	2.3576564894494873E-4	0.0	0.0	0.616291406342096	0.0
3	2.3576564894494873E-4	0.0	0.0	0.8786985736178239	0.0
4	2.3576564894494873E-4	0.0	0.0	1.3120358363786397	0.0
5	2.3576564894494873E-4	0.0	0.0	2.326063892490864	0.0
6	2.3576564894494873E-4	0.0	0.0	3.4325120829895086	0.0
7	2.3576564894494873E-4	0.0	0.0	4.339738300129671	0.0
8	2.3576564894494873E-4	0.0	0.0	5.649180714369916	0.0
9	2.3576564894494873E-4	0.0	0.0	6.314039844394672	0.0
10	2.3576564894494873E-4	0.0	0.0	7.763998585406107	0.0
11	2.3576564894494873E-4	0.0	0.0	11.010255805729106	0.0
12	2.3576564894494873E-4	0.0	0.0	13.780737946481198	0.0
13	2.3576564894494873E-4	0.0	0.0	14.774490156784157	0.0
14	2.3576564894494873E-4	0.0	0.0	15.131675114935755	0.0
15	2.3576564894494873E-4	0.0	0.0	15.575621831899092	0.0
16	2.3576564894494873E-4	0.0	0.0	16.724036307909937	2.3576564894494873E-4
17	2.3576564894494873E-4	0.0	0.0	18.30720264057527	2.3576564894494873E-4
18	2.3576564894494873E-4	0.0	0.0	19.854296828952023	2.3576564894494873E-4
19	2.3576564894494873E-4	0.0	0.0	20.78321348579512	2.3576564894494873E-4
20	2.3576564894494873E-4	0.0	0.0	21.605328303666155	2.3576564894494873E-4
21	2.3576564894494873E-4	0.0	0.0	22.58257691854297	2.3576564894494873E-4
22	2.3576564894494873E-4	0.0	0.0	23.417187315808086	2.3576564894494873E-4
23	2.3576564894494873E-4	0.0	0.0	24.078981492396558	2.3576564894494873E-4
24	2.3576564894494873E-4	0.0	0.0	24.500058941412235	2.3576564894494873E-4
25	2.3576564894494873E-4	0.0	0.0	24.819049864434753	2.3576564894494873E-4
26	2.3576564894494873E-4	0.0	0.0	25.070376046210068	2.3576564894494873E-4
27	2.3576564894494873E-4	0.0	0.0	25.302133679122953	2.3576564894494873E-4
28	2.3576564894494873E-4	0.0	0.0	25.527997170812213	2.3576564894494873E-4
29	2.3576564894494873E-4	0.0	0.0	25.77555110220441	2.3576564894494873E-4
30	2.3576564894494873E-4	0.0	0.0	26.010373688553578	2.3576564894494873E-4
31	2.3576564894494873E-4	0.0	0.0	26.241895555817518	2.3576564894494873E-4
32	2.3576564894494873E-4	0.0	0.0	26.515383708593657	2.3576564894494873E-4
33	2.3576564894494873E-4	0.0	0.0	26.72757279264411	2.3576564894494873E-4
34	2.3576564894494873E-4	0.0	0.0	26.931038547683603	2.3576564894494873E-4
35	2.3576564894494873E-4	0.0	0.0	27.137805021808322	2.3576564894494873E-4
36	2.3576564894494873E-4	0.0	0.0	27.343392667688317	2.3576564894494873E-4
37	2.3576564894494873E-4	0.0	0.0	27.597076505953083	2.3576564894494873E-4
38	2.3576564894494873E-4	0.0	0.0	27.841093952611104	2.3576564894494873E-4
39	2.3576564894494873E-4	0.0	0.0	28.088883649652246	2.3576564894494873E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACG	20	7.028247E-4	45.000004	9
CTATCGG	20	7.028247E-4	45.000004	2
AGGCACG	20	7.028247E-4	45.000004	10
CGTTTTT	6995	0.0	43.713364	1
GCGATCT	150	0.0	42.0	9
CGTTTGG	205	0.0	40.609756	2
GCGCGAC	50	1.0786607E-9	40.5	9
GCCGATT	45	1.9233994E-8	40.0	9
TGCGGGA	370	0.0	39.527027	4
ACAAGCG	245	0.0	39.489796	14
TTACGGG	80	0.0	39.375004	3
GCGGGAT	155	0.0	39.193546	5
TGGGCGA	590	0.0	38.898304	6
GTTTTTT	8295	0.0	38.598553	2
CGGGTAC	35	6.2389718E-6	38.571426	6
CCGCTTG	35	6.2389718E-6	38.571426	1
CTACGGG	35	6.2389718E-6	38.571426	3
TTGGGAC	750	0.0	37.8	5
CGGGTGC	30	1.13870745E-4	37.500004	6
CGGGTAG	60	1.546141E-10	37.500004	6
>>END_MODULE