##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933671.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425990
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13666283246086	33.0	31.0	34.0	30.0	34.0
2	32.35382990211038	34.0	31.0	34.0	30.0	34.0
3	32.49440127702528	34.0	31.0	34.0	31.0	34.0
4	35.96638888236813	37.0	35.0	37.0	35.0	37.0
5	36.044576163759714	37.0	35.0	37.0	35.0	37.0
6	36.03861358247846	37.0	35.0	37.0	35.0	37.0
7	36.06237470363154	37.0	35.0	37.0	35.0	37.0
8	35.98350900255875	37.0	35.0	37.0	35.0	37.0
9	37.82997018709359	39.0	38.0	39.0	35.0	39.0
10	37.11821404258316	39.0	37.0	39.0	33.0	39.0
11	37.10112209206789	39.0	37.0	39.0	34.0	39.0
12	37.1828235404587	39.0	37.0	39.0	34.0	39.0
13	37.241660602361556	39.0	37.0	39.0	34.0	39.0
14	38.49787553698444	40.0	38.0	41.0	34.0	41.0
15	38.51485011385244	40.0	38.0	41.0	34.0	41.0
16	38.6087537266133	40.0	38.0	41.0	34.0	41.0
17	38.59615953426137	40.0	38.0	41.0	34.0	41.0
18	38.346073851498865	39.0	38.0	41.0	34.0	41.0
19	38.093046785135805	39.0	37.0	41.0	34.0	41.0
20	37.67344538604193	39.0	35.0	41.0	34.0	41.0
21	37.578248315688164	39.0	35.0	41.0	34.0	41.0
22	37.56153196084415	39.0	35.0	41.0	34.0	41.0
23	37.43686941008005	39.0	35.0	41.0	33.0	41.0
24	37.33377778821099	39.0	35.0	41.0	33.0	41.0
25	37.2693983426841	39.0	35.0	41.0	33.0	41.0
26	37.23644451747693	39.0	35.0	41.0	33.0	41.0
27	37.17062607103453	39.0	35.0	41.0	33.0	41.0
28	37.084741425855064	39.0	35.0	41.0	33.0	41.0
29	36.855562337144065	39.0	35.0	41.0	33.0	41.0
30	36.58317096645461	39.0	35.0	41.0	31.0	41.0
31	36.25254113946337	39.0	35.0	41.0	30.0	41.0
32	35.85971736425738	39.0	35.0	41.0	28.0	41.0
33	35.285687457452056	39.0	35.0	41.0	23.0	41.0
34	34.83552665555529	39.0	35.0	41.0	18.0	41.0
35	34.55193549144346	39.0	35.0	41.0	17.0	41.0
36	34.38171553322848	39.0	35.0	41.0	15.0	41.0
37	34.30679593417686	39.0	34.0	41.0	15.0	41.0
38	34.21698161928684	39.0	34.0	41.0	15.0	41.0
39	34.192812037841264	39.0	34.0	41.0	15.0	41.0
40	34.04539073687176	39.0	34.0	41.0	13.0	41.0
41	33.92469306791239	38.0	34.0	41.0	12.0	41.0
42	33.758130472546306	38.0	33.0	41.0	10.0	41.0
43	33.69910561280781	38.0	33.0	41.0	10.0	41.0
44	33.50257752529402	38.0	33.0	40.0	10.0	41.0
45	33.44308082349351	38.0	33.0	40.0	10.0	41.0
46	33.23165097772248	38.0	33.0	40.0	10.0	41.0
47	33.19239653512993	38.0	33.0	40.0	10.0	41.0
48	33.08256766590765	38.0	33.0	40.0	10.0	41.0
49	33.07036315406465	38.0	33.0	40.0	10.0	41.0
50	32.903518861945116	37.0	32.0	40.0	10.0	41.0
51	32.04635789572525	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	8.0
11	6.0
12	4.0
13	12.0
14	14.0
15	17.0
16	43.0
17	79.0
18	141.0
19	296.0
20	500.0
21	892.0
22	1559.0
23	2683.0
24	5032.0
25	9199.0
26	12692.0
27	12854.0
28	10797.0
29	9218.0
30	8778.0
31	9194.0
32	10487.0
33	13415.0
34	20357.0
35	26734.0
36	33996.0
37	46778.0
38	85031.0
39	105127.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.93058522500528	5.005986056010705	41.21599098570389	30.847437733280124
2	34.89518533298904	4.7339139416418226	44.88110049531679	15.489800230052348
3	18.840113617690555	4.857860513157586	59.43402427287025	16.868001596281605
4	14.90856592877767	4.922885513744454	59.77745956477851	20.39108899269936
5	13.590225122655461	5.630179112185732	61.36622925420785	19.41336651095096
6	17.664733913941642	6.654616305547078	62.54090471607314	13.139745064438133
7	70.71292753351017	2.0258691518580245	23.93013920514566	3.3310641094861384
8	71.99910796028077	2.1047442428226013	22.298645508110518	3.5975022887861217
9	66.83419798586822	4.785558346440057	24.006666823164863	4.373576844526867
10	29.342707575295197	20.708702082208504	33.22120237564262	16.727387966853684
11	20.77278809361722	22.411558956783022	36.917063780839925	19.89858916875983
12	17.05274771708256	20.509166881851687	43.25406699687786	19.184018404187892
13	18.957722012253807	20.481231953801732	45.11232658043616	15.448719453508296
14	18.631658020141316	21.61130542970492	44.081316462827765	15.675720087325992
15	15.97666611892298	21.847226460715042	45.06537712152868	17.110730298833307
16	16.657668020376065	20.917392427052278	42.973543979905635	19.451395572666026
17	17.081621634310665	21.1345336744994	41.31223737646423	20.4716073147257
18	18.273433648677198	19.661729148571563	42.87495011619991	19.189887086551327
19	17.866381839949295	23.066503908542455	41.87281391581962	17.19430033568863
20	19.227681400971854	23.098429540599543	40.56433249606798	17.10955656236062
21	18.3957369891312	24.464189300218315	40.828657949717126	16.311415760933357
22	17.410267846663068	22.490199300453064	39.9561022559215	20.143430596962368
23	16.779267118946454	23.838587760276063	38.78612173994695	20.596023380830538
24	16.59710321838541	23.889762670485222	39.54787671072091	19.965257400408458
25	16.341698161928683	25.865161154017702	37.78140331932674	20.011737364726873
26	15.858118735181579	26.63982722599122	38.629545294490484	18.87250874433672
27	16.251085706237237	25.42031503086927	39.62158736120566	18.707011901687835
28	15.415385337683984	24.240240381229604	39.95657175051058	20.387802530575836
29	16.887250874433672	23.41768586117045	38.518744571468815	21.176318692927065
30	19.24082724946595	24.093288574849176	37.12105917979295	19.544824995891922
31	18.355360454470762	26.654146810958	34.84095870795089	20.149534026620344
32	16.814948707716145	26.562125871499333	34.63414634146341	21.988779079321112
33	19.659616422920724	24.34705040024414	32.35827132092302	23.63506185591211
34	18.444564426394987	25.513040212211553	32.53128007699711	23.511115284396347
35	17.98258175074532	26.90274419587314	31.25402004741895	23.86065400596258
36	18.938237986807202	27.61567172938332	30.999553980140377	22.446536303669102
37	17.86497335618207	26.29967839620648	33.46487006737247	22.370478180238972
38	18.92743961125848	25.709523697739385	32.373060400478884	22.989976290523252
39	21.984788375313975	24.103852203103358	30.53616282072349	23.375196600859173
40	21.927744782741378	24.808094086715652	30.634052442545602	22.63010868799737
41	18.469917134205026	25.703889762670485	32.33315336040752	23.493039742716963
42	18.131646282776593	26.267048522265778	34.929693185286034	20.671612009671588
43	18.43752200755886	24.394704101035238	35.767975774079204	21.3997981173267
44	19.89671119040353	24.50386159299514	31.98408413343036	23.615343083170966
45	21.664827812859457	23.68248080940867	30.241789713373553	24.410901664358317
46	21.540881241343694	24.381088757952067	31.90826075729477	22.16976924340947
47	18.368271555670322	26.049907274818658	34.61325383224958	20.968567337261437
48	18.22225873846804	25.11396981149792	35.440972792788564	21.222798657245477
49	20.874668419446465	23.281062935749667	34.528979553510645	21.315289091293224
50	20.08756074086246	22.57916852508275	32.8014742130097	24.531796521045095
51	19.23542806169159	22.18807953238339	31.806380431465524	26.770111974459493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	506.0
1	1055.5
2	1605.0
3	10067.0
4	18529.0
5	12434.0
6	6339.0
7	6138.0
8	5937.0
9	5925.5
10	5914.0
11	5830.5
12	5747.0
13	5589.5
14	5432.0
15	5175.5
16	4919.0
17	4824.5
18	4730.0
19	4485.5
20	4241.0
21	4128.0
22	4015.0
23	4012.0
24	4009.0
25	4039.5
26	4291.5
27	4513.0
28	4858.0
29	5203.0
30	5766.0
31	6329.0
32	7326.0
33	8323.0
34	9453.5
35	10584.0
36	11690.0
37	12796.0
38	14551.5
39	16307.0
40	17572.0
41	18837.0
42	20989.0
43	23141.0
44	25041.5
45	26942.0
46	30770.0
47	34598.0
48	38146.0
49	41694.0
50	40309.0
51	38924.0
52	33581.0
53	28238.0
54	23953.0
55	19668.0
56	16657.0
57	13646.0
58	12484.0
59	11322.0
60	10002.0
61	8682.0
62	7393.0
63	6104.0
64	5444.0
65	4784.0
66	4007.0
67	3230.0
68	2585.0
69	1940.0
70	1687.5
71	1435.0
72	1258.0
73	1081.0
74	953.0
75	658.5
76	492.0
77	339.5
78	187.0
79	133.5
80	80.0
81	70.0
82	60.0
83	39.5
84	19.0
85	13.0
86	7.0
87	5.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	425990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.888111452744816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99243806931143	36.662386704455606
2	9.989709222394218	9.767560356898425
3	4.560519410639555	6.68865543689258
4	2.755856601903755	5.389144988066135
5	1.8451001809627559	4.510173164419342
6	1.2486373504542052	3.6626113171839108
7	0.9263044323657182	3.169969203006673
8	0.7306956377095738	2.8577863819504077
9	0.5291870395908171	2.3283859473777557
>10	2.3433285931847254	18.0155626091254
>50	0.05408041781564659	1.811064303103037
>100	0.02124587842753137	1.6642340115818575
>500	0.0019314434934119425	0.6312286165914733
>1k	4.8286087335298563E-4	0.3810033609493785
>5k	0.0	0.0
>10k+	4.8286087335298563E-4	2.4602335983980312
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10422	2.4465363036691	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	1614	0.37888213338341276	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	778	0.1826333951501209	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	689	0.16174088593628957	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	663	0.1556374562783164	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	544	0.12770252822836217	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.694945890748609E-4	0.0	0.0	0.17300875607408625	0.0
2	4.694945890748609E-4	0.0	0.0	0.5899199511725627	0.0
3	4.694945890748609E-4	0.0	0.0	0.8476724805746614	0.0
4	4.694945890748609E-4	0.0	0.0	1.2817202281743703	0.0
5	4.694945890748609E-4	0.0	0.0	2.2876123852672596	0.0
6	7.042418836122914E-4	0.0	0.0	3.3646329726049906	0.0
7	7.042418836122914E-4	0.0	0.0	4.262306626916125	0.0
8	7.042418836122914E-4	0.0	0.0	5.596375501772342	0.0
9	7.042418836122914E-4	0.0	0.0	6.26892650062208	0.0
10	9.389891781497219E-4	0.0	0.0	7.68773914880631	0.0
11	9.389891781497219E-4	0.0	0.0	10.87795488156999	0.0
12	9.389891781497219E-4	0.0	0.0	13.585060682175637	0.0
13	9.389891781497219E-4	0.0	0.0	14.54517711683373	0.0
14	9.389891781497219E-4	0.0	0.0	14.898471795112561	0.0
15	9.389891781497219E-4	0.0	0.0	15.317730463156412	0.0
16	0.0011737364726871523	0.0	0.0	16.453203126833962	0.0
17	0.0011737364726871523	0.0	0.0	17.97999953050541	0.0
18	0.0011737364726871523	0.0	0.0	19.488720392497477	0.0
19	0.0011737364726871523	0.0	0.0	20.40564332496068	0.0
20	0.0011737364726871523	0.0	0.0	21.187351815770324	0.0
21	0.0014084837672245828	0.0	0.0	22.167421770464095	0.0
22	0.0014084837672245828	0.0	0.0	23.01931970234043	0.0
23	0.0014084837672245828	0.0	0.0	23.658301838071317	0.0
24	0.0014084837672245828	0.0	0.0	24.081316462827765	0.0
25	0.0014084837672245828	0.0	0.0	24.39540834291885	0.0
26	0.0014084837672245828	0.0	0.0	24.63179886851804	0.0
27	0.0014084837672245828	0.0	0.0	24.848470621376087	0.0
28	0.0014084837672245828	0.0	0.0	25.05856944998709	0.0
29	0.0014084837672245828	0.0	0.0	25.30599309842954	0.0
30	0.0014084837672245828	0.0	0.0	25.538862414610673	0.0
31	0.0016432310617620132	0.0	0.0	25.771262236202727	0.0
32	0.0018779783562994437	0.0	0.0	26.029953754782976	0.0
33	0.0018779783562994437	0.0	0.0	26.254841662949836	0.0
34	0.0018779783562994437	0.0	0.0	26.446630202586917	0.0
35	0.0018779783562994437	0.0	0.0	26.65532054743069	0.0
36	0.0018779783562994437	0.0	0.0	26.859315946383717	0.0
37	0.0018779783562994437	0.0	0.0	27.09852343951736	0.0
38	0.0018779783562994437	0.0	0.0	27.334913965116552	0.0
39	0.0018779783562994437	0.0	0.0	27.58538932838799	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8866565E-5	45.000004	1
GCCGATC	30	2.1622727E-6	45.000004	9
TACGCGC	20	7.0282805E-4	45.000004	34
ATACGCG	20	7.0282805E-4	45.000004	33
TGTCGTG	20	7.0282805E-4	45.000004	1
ATTTCGC	20	7.0282805E-4	45.000004	12
CGTTTTT	6430	0.0	43.74028	1
CGCGGGA	85	0.0	42.35294	4
TGTTGCG	85	0.0	42.35294	1
GCCGATT	85	0.0	42.35294	9
GGCCGAT	165	0.0	42.272728	8
ACACGCG	150	0.0	42.0	36
TTACACG	150	0.0	42.0	34
TACGGGA	60	3.6379788E-12	41.250004	4
CGGGTAT	105	0.0	40.714287	6
TTGTGCG	50	1.0786607E-9	40.500004	1
GGGCCGA	175	0.0	39.857143	7
TGGGCGA	615	0.0	39.14634	6
CTTTGCG	185	0.0	38.91892	1
CGGGTCC	70	0.0	38.571426	6
>>END_MODULE