FastQCFastQC Report
Sat 14 Jan 2017
SRR2933670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054939
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430114.077107775899838No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10810.10247037980395075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA351.2117926E-745.022
CGCTATC302.1653323E-644.99999626
CGTTTTT276150.044.291151
TACGGGT800.042.1875044
GTTTTTT310700.040.307372
GATAACG403.4578625E-739.37500417
CGATAAC403.4578625E-739.37500416
GCTACGA1100.038.86363610
CCGATAA356.247803E-638.57142615
CCGCTAT356.247803E-638.57142625
TACCGGT356.247803E-638.57142640
TGGGCGA15950.037.8056456
CGTGCAA301.13985836E-437.49999614
TCGTTTG2900.037.2413831
TACGAAT1150.037.17391212
TTGGGAC15800.036.4556965
TTTGGGC28950.036.1398964
CTCGTAA751.8189894E-1236.00000433
CGAATAT1250.036.014
TTACGTA250.002107030236.020
Produced by FastQC (version 0.11.5)