##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1054939
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52657926192889	34.0	31.0	34.0	31.0	34.0
2	32.712783393163015	34.0	31.0	34.0	31.0	34.0
3	32.753701398848655	34.0	31.0	34.0	31.0	34.0
4	36.23021520675603	37.0	37.0	37.0	35.0	37.0
5	36.233426766855715	37.0	37.0	37.0	35.0	37.0
6	36.20841868581975	37.0	37.0	37.0	35.0	37.0
7	36.264315756645644	37.0	37.0	37.0	35.0	37.0
8	36.16822868431255	37.0	37.0	37.0	35.0	37.0
9	38.01650332388887	39.0	39.0	39.0	35.0	39.0
10	37.43774474163909	39.0	37.0	39.0	34.0	39.0
11	37.38657495836252	39.0	37.0	39.0	34.0	39.0
12	37.37207269804226	39.0	37.0	39.0	34.0	39.0
13	37.38223063134456	39.0	37.0	39.0	34.0	39.0
14	38.70370040353044	40.0	38.0	41.0	35.0	41.0
15	38.76779984435119	40.0	38.0	41.0	35.0	41.0
16	38.79662710355765	40.0	38.0	41.0	35.0	41.0
17	38.74210167602108	40.0	38.0	41.0	34.0	41.0
18	38.26535373135319	39.0	38.0	41.0	34.0	41.0
19	37.778882949630265	38.0	37.0	41.0	34.0	41.0
20	37.222751268082796	38.0	35.0	41.0	34.0	41.0
21	37.1318000377273	38.0	35.0	40.0	33.0	41.0
22	37.11490048239756	38.0	35.0	40.0	33.0	41.0
23	37.03958617512482	38.0	35.0	40.0	33.0	41.0
24	36.92926510442784	38.0	35.0	40.0	33.0	41.0
25	36.85165966942164	38.0	35.0	40.0	33.0	41.0
26	36.782887920533796	38.0	35.0	40.0	33.0	41.0
27	36.707518633778825	38.0	35.0	40.0	33.0	41.0
28	36.5253545465662	38.0	35.0	40.0	33.0	41.0
29	36.32784644420199	38.0	35.0	40.0	32.0	41.0
30	36.05939869509043	38.0	35.0	40.0	31.0	41.0
31	35.59866020689348	38.0	35.0	40.0	30.0	41.0
32	34.83110303060177	38.0	35.0	40.0	23.0	41.0
33	33.90935305264096	38.0	34.0	40.0	16.0	41.0
34	33.07669922147157	38.0	33.0	40.0	12.0	41.0
35	32.51664409032181	37.0	32.0	40.0	9.0	41.0
36	32.21826664859295	37.0	31.0	40.0	8.0	41.0
37	32.008640310008445	37.0	31.0	40.0	7.0	41.0
38	31.890780414791756	37.0	30.0	40.0	7.0	41.0
39	31.800400781466987	37.0	30.0	40.0	7.0	41.0
40	31.66269518901093	37.0	29.0	40.0	7.0	41.0
41	31.48854957490433	37.0	29.0	40.0	7.0	41.0
42	31.32346135653341	36.0	27.0	40.0	7.0	41.0
43	31.180241701178932	36.0	27.0	40.0	7.0	41.0
44	30.965299415416435	36.0	25.0	40.0	7.0	41.0
45	30.96028585539069	36.0	25.0	40.0	7.0	41.0
46	30.83570803619925	35.0	24.0	40.0	7.0	41.0
47	30.751138217470395	35.0	24.0	40.0	7.0	41.0
48	30.66652858601303	35.0	24.0	40.0	7.0	41.0
49	30.65014754407601	35.0	24.0	40.0	7.0	41.0
50	30.556232161290843	35.0	24.0	40.0	7.0	41.0
51	29.689626604002697	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	11.0
12	11.0
13	16.0
14	25.0
15	60.0
16	93.0
17	216.0
18	439.0
19	909.0
20	1581.0
21	2501.0
22	4110.0
23	7285.0
24	14847.0
25	30255.0
26	49483.0
27	52196.0
28	42119.0
29	32581.0
30	27005.0
31	26241.0
32	28197.0
33	35076.0
34	54198.0
35	65061.0
36	80200.0
37	103920.0
38	179717.0
39	216497.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.354447034378293	3.621915579952964	37.66577972754823	38.357857658120516
2	43.156049781077385	3.767706000062563	39.9560543311035	13.120189887756542
3	16.70115523267222	3.7717820651241447	65.18594914018725	14.341113562016384
4	13.799376077668946	4.092464114038822	64.9822406793189	17.125919128973337
5	12.980371376923216	4.8285256303918995	64.655207552285	17.53589544039987
6	16.010973146314623	5.6093290702116425	66.29407008367309	12.085627699800652
7	63.515236425992406	1.842097031202752	31.78420742810722	2.8584591146976273
8	65.22936397270364	2.0346200112044395	30.26023305612931	2.475782959962614
9	60.93025283926369	3.7779435588218844	31.676428684502138	3.6153749174122862
10	29.52445591640844	17.531534998706086	39.36170716979844	13.582301915087033
11	22.046393203777658	19.50690987820149	42.678960584450856	15.767736333569998
12	17.412760358655806	17.857620203632628	48.153495130998095	16.576124306713467
13	17.545469453684053	17.49722021842021	49.6912143735325	15.266095954363237
14	16.53195113651121	18.308451957885715	49.92336049762119	15.236236407981885
15	15.81494285451576	18.230153591819054	50.03360383870537	15.921299714959822
16	17.168006870539436	17.4793044905914	49.133077836728	16.219610802141165
17	17.35455794126485	17.940752972446745	47.81385463993652	16.890834446351874
18	18.23024838402979	17.405082189586317	47.98940981421674	16.37525961216715
19	18.470262261609438	19.847498291370403	45.64197550758859	16.04026393943157
20	19.292774274152343	20.100688286242143	45.40499498075244	15.201542458853071
21	19.608811504741034	19.9875063866252	45.09189630869652	15.311785799937248
22	18.64164657861734	18.323618711603228	45.66681106680102	17.367923642978408
23	17.589547831675574	19.893188136944413	45.2832817821694	17.233982249210616
24	17.109235699884067	19.883519331449495	46.02313498695185	16.98410998171458
25	17.284790874164287	20.908507506121207	44.69793988088411	17.108761738830395
26	16.653569542883524	20.690675005853418	45.059382580414606	17.596372870848455
27	16.990650644255258	19.85233269411786	45.658943313310054	17.498073348316822
28	16.761822247542273	20.14969585919186	45.364423914558095	17.724057978707776
29	17.707469341829242	19.950253047806555	44.60807686510784	17.734200745256363
30	18.60145468126593	20.38629721718507	44.06842480939656	16.943823292152437
31	18.9636557184823	21.218857204065827	42.548810879112445	17.26867619833943
32	18.83966750684163	21.39858323561836	41.658238059262196	18.103511198277815
33	20.575976430864724	21.80647411840874	38.94784437773179	18.66970507299474
34	19.47676595518793	23.098112781876488	38.05006734986573	19.375053913069856
35	20.293780019508237	24.486344708082648	36.094693626835294	19.125181645573818
36	21.5813426179144	25.755138448763386	33.757686463387934	18.905832469934282
37	21.041974938835324	26.107291511641904	34.42919448423084	18.421539065291928
38	21.233644788940403	25.393316580389957	34.57176196917547	18.80127666149417
39	21.285780504844357	25.230937523401824	33.42126890749134	20.062013064262484
40	21.74457480479914	25.01500086734873	33.78138451607155	19.45903981178059
41	20.478435246018964	25.93249467504756	33.679482889532004	19.909587189401474
42	20.762622293800874	26.567128525914768	33.31017243651055	19.36007674377381
43	20.200125315302593	25.448296062615945	34.46057070598395	19.891007916097518
44	20.8069850484246	25.249611588916515	33.57170414592692	20.37169921673196
45	20.960453637603692	24.705599091511452	33.129498482850664	21.20444878803419
46	20.746318033554548	25.05244379058884	33.39558021838229	20.80565795747432
47	19.93821443704328	25.85400672455943	34.12984068273141	20.077938155665873
48	20.00494815340034	25.226387497286574	34.51697207137095	20.251692277942137
49	20.69408752543986	24.534119982292815	34.09998113635007	20.67181135591726
50	20.02068366038226	24.401979640528978	34.047181874970974	21.53015482411779
51	19.674502506780012	24.24178080438774	33.73683217702635	22.346884511805897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	641.0
1	1877.0
2	3113.0
3	36769.5
4	70426.0
5	46820.5
6	23215.0
7	22959.0
8	22703.0
9	22756.5
10	22810.0
11	22664.5
12	22519.0
13	21670.0
14	20821.0
15	20020.0
16	19219.0
17	18232.0
18	17245.0
19	16494.5
20	15744.0
21	14911.0
22	14078.0
23	13733.0
24	13388.0
25	13152.0
26	13029.5
27	13143.0
28	13719.5
29	14296.0
30	15084.5
31	15873.0
32	18055.0
33	20237.0
34	22401.0
35	24565.0
36	26497.0
37	28429.0
38	30663.5
39	32898.0
40	34130.0
41	35362.0
42	38565.0
43	41768.0
44	45998.0
45	50228.0
46	54598.0
47	58968.0
48	63390.5
49	67813.0
50	67880.0
51	67947.0
52	63194.0
53	58441.0
54	53558.5
55	48676.0
56	45303.5
57	41931.0
58	39245.0
59	36559.0
60	34235.5
61	31912.0
62	28350.0
63	24788.0
64	21550.0
65	18312.0
66	16068.5
67	13825.0
68	11793.0
69	9761.0
70	8458.0
71	7155.0
72	6276.0
73	5397.0
74	4416.0
75	2901.0
76	2367.0
77	1715.0
78	1063.0
79	810.0
80	557.0
81	379.0
82	201.0
83	156.0
84	111.0
85	78.0
86	45.0
87	29.5
88	14.0
89	16.5
90	19.0
91	11.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1054939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.80095488706823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.31074169534328	31.89861871005761
2	7.786619766484638	6.509762831631563
3	3.656248031171471	4.5850397702079215
4	2.386943231166528	3.991060252959397
5	1.6364485211625937	3.4202555404063557
6	1.297948489800194	3.2553291760725753
7	0.998299608809591	2.921091383813931
8	0.8636796577539738	2.888210752852191
9	0.7271920716884007	2.735759068459248
>10	4.228932510996227	28.507919541879062
>50	0.07016451139327604	2.044765406676523
>100	0.0338302700167068	2.3249750480483
>500	0.002270487856241859	0.6349382694131908
>1k	4.540975712483717E-4	0.20016215398989826
>5k	0.0	0.0
>10k+	2.2704878562418586E-4	4.082112093532249
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43011	4.077107775899838	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1081	0.10247037980395075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8958442146891906E-4	0.0	0.0	0.05374718348643855	0.0
2	1.8958442146891906E-4	0.0	0.0	0.1695832650039481	0.0
3	1.8958442146891906E-4	0.0	0.0	0.2915808402191975	0.0
4	1.8958442146891906E-4	0.0	0.0	0.4433431696050672	0.0
5	1.8958442146891906E-4	0.0	0.0	0.8200474150638094	0.0
6	1.8958442146891906E-4	0.0	0.0	1.528145229250222	0.0
7	1.8958442146891906E-4	0.0	0.0	2.1235351048733624	0.0
8	1.8958442146891906E-4	0.0	0.0	2.937231441817963	0.0
9	1.8958442146891906E-4	0.0	0.0	3.580396591651271	0.0
10	1.8958442146891906E-4	0.0	0.0	4.357977096306042	0.0
11	1.8958442146891906E-4	0.0	0.0	5.4313092984523275	0.0
12	1.8958442146891906E-4	0.0	0.0	6.271357869981108	0.0
13	1.8958442146891906E-4	0.0	0.0	6.638488102155669	0.0
14	1.8958442146891906E-4	0.0	0.0	6.841153848705944	0.0
15	1.8958442146891906E-4	0.0	0.0	7.009504814970344	0.0
16	1.8958442146891906E-4	0.0	0.0	7.292838732855643	0.0
17	1.8958442146891906E-4	0.0	0.0	7.646413678895178	0.0
18	1.8958442146891906E-4	0.0	0.0	8.010605352536972	0.0
19	2.843766322033786E-4	0.0	0.0	8.253462996438657	0.0
20	2.843766322033786E-4	0.0	0.0	8.495941471497405	0.0
21	2.843766322033786E-4	0.0	0.0	8.77453577884598	0.0
22	2.843766322033786E-4	0.0	0.0	9.048200891236366	0.0
23	2.843766322033786E-4	0.0	0.0	9.293238755984943	0.0
24	2.843766322033786E-4	0.0	0.0	9.490785723155557	0.0
25	2.843766322033786E-4	0.0	0.0	9.656103338676454	0.0
26	2.843766322033786E-4	0.0	0.0	9.816776135871363	0.0
27	2.843766322033786E-4	0.0	0.0	9.98143020591712	0.0
28	2.843766322033786E-4	0.0	0.0	10.147980120177564	0.0
29	2.843766322033786E-4	0.0	0.0	10.324198839932926	0.0
30	2.843766322033786E-4	0.0	0.0	10.525347911111448	0.0
31	2.843766322033786E-4	0.0	0.0	10.714079202683758	0.0
32	2.843766322033786E-4	0.0	0.0	10.92309602735324	0.0
33	3.791688429378381E-4	0.0	0.0	11.12225446210634	0.0
34	3.791688429378381E-4	0.0	0.0	11.32416187097074	0.0
35	3.791688429378381E-4	0.0	0.0	11.532135981322142	0.0
36	3.791688429378381E-4	0.0	0.0	11.732242338182587	0.0
37	3.791688429378381E-4	0.0	0.0	11.943534175909697	0.0
38	4.7396105367229763E-4	0.0	0.0	12.18421159896449	0.0
39	4.7396105367229763E-4	0.0	0.0	12.514657245584816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	35	1.2117926E-7	45.0	22
CGCTATC	30	2.1653323E-6	44.999996	26
CGTTTTT	27615	0.0	44.29115	1
TACGGGT	80	0.0	42.187504	4
GTTTTTT	31070	0.0	40.30737	2
GATAACG	40	3.4578625E-7	39.375004	17
CGATAAC	40	3.4578625E-7	39.375004	16
GCTACGA	110	0.0	38.863636	10
CCGATAA	35	6.247803E-6	38.571426	15
CCGCTAT	35	6.247803E-6	38.571426	25
TACCGGT	35	6.247803E-6	38.571426	40
TGGGCGA	1595	0.0	37.805645	6
CGTGCAA	30	1.13985836E-4	37.499996	14
TCGTTTG	290	0.0	37.241383	1
TACGAAT	115	0.0	37.173912	12
TTGGGAC	1580	0.0	36.455696	5
TTTGGGC	2895	0.0	36.139896	4
CTCGTAA	75	1.8189894E-12	36.000004	33
CGAATAT	125	0.0	36.0	14
TTACGTA	25	0.0021070302	36.0	20
>>END_MODULE