FastQCFastQC Report
Sat 14 Jan 2017
SRR2933669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067579
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426233.9924914221804664No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11300.10584696776538317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG207.0330594E-445.00000424
TATTACG207.0330594E-445.0000041
CAGTACG207.0330594E-445.00000421
CGTTTTT272700.044.2574271
CGAATAT1750.039.85714314
GTTTTTT312000.039.7283632
CGTTCAT403.457917E-739.37500417
TACGAAT1800.038.75000412
ACCGGAA356.247872E-638.57142628
GGTATCA659.094947E-1238.076928
TACCGGG950.037.8947373
TGGGCGA18150.037.8099176
CTACGAA1850.037.702711
ACGGTAT301.1398674E-437.50000440
GCGATCT3300.036.818189
GCTACGA1900.036.71052610
TTGGGAC15400.036.233775
ACCCGCT751.8189894E-1236.034
ACGACGG250.002107041236.025
TTGGGCG15400.035.941565
Produced by FastQC (version 0.11.5)