##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1067579
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40843066414757	34.0	31.0	34.0	31.0	34.0
2	32.63441394032667	34.0	31.0	34.0	31.0	34.0
3	32.690982119356036	34.0	31.0	34.0	31.0	34.0
4	36.14848268840058	37.0	35.0	37.0	35.0	37.0
5	36.20372356518815	37.0	35.0	37.0	35.0	37.0
6	36.19696247303478	37.0	37.0	37.0	35.0	37.0
7	36.19175723763768	37.0	37.0	37.0	35.0	37.0
8	36.126696010318675	37.0	37.0	37.0	35.0	37.0
9	37.98463626579391	39.0	39.0	39.0	35.0	39.0
10	37.44868997985161	39.0	37.0	39.0	34.0	39.0
11	37.39712283587444	39.0	37.0	39.0	34.0	39.0
12	37.35915843230337	39.0	37.0	39.0	34.0	39.0
13	37.37960001086571	39.0	37.0	39.0	34.0	39.0
14	38.730113649669015	40.0	38.0	41.0	35.0	41.0
15	38.781411961082036	40.0	38.0	41.0	35.0	41.0
16	38.85341412672973	40.0	38.0	41.0	35.0	41.0
17	38.82280936586426	40.0	38.0	41.0	35.0	41.0
18	38.382159072068674	39.0	38.0	41.0	35.0	41.0
19	37.92117023658202	39.0	37.0	41.0	35.0	41.0
20	37.31766080074636	38.0	35.0	41.0	34.0	41.0
21	37.266577930064194	38.0	35.0	41.0	34.0	41.0
22	37.22451827920931	38.0	35.0	41.0	34.0	41.0
23	37.0978681671333	38.0	35.0	41.0	33.0	41.0
24	36.99389178693099	38.0	35.0	41.0	33.0	41.0
25	36.930131634286546	38.0	35.0	40.0	33.0	41.0
26	36.89073595490357	38.0	35.0	40.0	33.0	41.0
27	36.820114483330975	38.0	35.0	40.0	33.0	41.0
28	36.70417926916884	38.0	35.0	40.0	33.0	41.0
29	36.45630534133774	38.0	35.0	40.0	33.0	41.0
30	36.10724733251591	38.0	35.0	40.0	31.0	41.0
31	35.63507899649581	38.0	35.0	40.0	30.0	41.0
32	34.95199231157601	38.0	35.0	40.0	23.0	41.0
33	33.97351484058791	38.0	34.0	40.0	16.0	41.0
34	33.161555257269015	38.0	33.0	40.0	12.0	41.0
35	32.64712025995266	38.0	32.0	40.0	10.0	41.0
36	32.331958571684154	37.0	31.0	40.0	8.0	41.0
37	32.144234759207514	37.0	31.0	40.0	8.0	41.0
38	32.010702720829094	37.0	30.0	40.0	7.0	41.0
39	31.950221014088886	37.0	30.0	40.0	7.0	41.0
40	31.80611645601871	37.0	30.0	40.0	7.0	41.0
41	31.647777822531165	37.0	29.0	40.0	7.0	41.0
42	31.43793293049039	36.0	28.0	40.0	7.0	41.0
43	31.313230215281493	36.0	27.0	40.0	7.0	41.0
44	31.108928706915368	36.0	26.0	40.0	7.0	41.0
45	31.080682553703287	36.0	26.0	40.0	7.0	41.0
46	30.94161462524085	35.0	25.0	40.0	7.0	41.0
47	30.916746208008963	35.0	25.0	40.0	7.0	41.0
48	30.797937201837055	35.0	24.0	40.0	7.0	41.0
49	30.774363302387926	35.0	25.0	40.0	7.0	41.0
50	30.621112816943757	35.0	24.0	40.0	7.0	41.0
51	29.857089732937798	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	5.0
10	9.0
11	11.0
12	22.0
13	20.0
14	38.0
15	50.0
16	117.0
17	200.0
18	458.0
19	918.0
20	1511.0
21	2501.0
22	4155.0
23	7149.0
24	14715.0
25	30696.0
26	49480.0
27	52375.0
28	42381.0
29	32311.0
30	26927.0
31	26131.0
32	27843.0
33	34530.0
34	53581.0
35	65252.0
36	79331.0
37	101911.0
38	177818.0
39	235014.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.41113585036798	3.8972291511916217	37.55750159941325	38.13413339902714
2	43.20401581522304	3.8347513392451518	39.8000522677947	13.161180577737106
3	16.848589191057524	3.8462727348514725	64.93889445183916	14.366243622251842
4	13.947164565807308	4.125221646360598	64.74321806629767	17.184395721534425
5	13.016273268769805	4.921509321558404	64.56861740442628	17.49360000524551
6	16.012304475828017	5.588719898012232	66.21505293753437	12.183922688625385
7	63.48251511129387	1.894379713351424	31.719713482561946	2.9033916927927583
8	65.27441997266713	2.0523071360526948	30.165542784187398	2.507730107092777
9	60.93890943902044	3.850300539819536	31.597005935860484	3.613784085299542
10	29.547883575829047	17.622115084691625	39.23915700852115	13.590844330958177
11	22.07134085627387	19.518742875234526	42.60471590392842	15.805200364563184
12	17.429904484820327	17.84636078454147	48.05414868595205	16.669586044686156
13	17.53856154907506	17.567411873032345	49.634734291326446	15.259292286566145
14	16.60223739882482	18.38393224295345	49.75847220674067	15.25535815148106
15	15.810445878009965	18.26534617110303	50.01522135598396	15.908986594903046
16	17.164444036460065	17.478612823969	49.087046485552825	16.26989665401811
17	17.474022999703067	18.005693255487415	47.65427195551805	16.86601178929147
18	18.323140488900588	17.35000407463991	47.88188977115511	16.444965665304395
19	18.476571757218903	19.93304476764717	45.60055977122068	15.989823703913247
20	19.45504735480934	20.058187731306067	45.249204040169396	15.2375608737152
21	19.681166452318752	19.995428909710665	44.953300879841215	15.370103758129375
22	18.73397659564304	18.31199377282618	45.59006874432712	17.363960887203664
23	17.753159250978147	19.811273919775495	45.21145507732917	17.22411175191719
24	17.259518967682954	19.87646815832833	45.978705088803736	16.885307785184985
25	17.364991255916422	20.936342884226836	44.602694507853755	17.09597135200299
26	16.740400476217683	20.755185330546965	44.90440520092658	17.600008992308766
27	17.050916138290468	19.86438474342414	45.52815295167852	17.556546166606875
28	16.88390273694031	20.207403854890362	45.20077671066966	17.70791669749967
29	17.698081359786958	20.056220663763526	44.46218968338643	17.78350829306309
30	18.669157036622114	20.371419819985217	43.97716702932523	16.982256114067436
31	18.981358756588506	21.297533952990833	42.519944659833136	17.201162630587525
32	18.922534070078186	21.409656802915755	41.54914999264692	18.118659134359145
33	20.635568889983784	21.93364612829589	38.78232898923639	18.648455992483928
34	19.565577816723632	23.176739145299784	37.91653826086875	19.34114477710783
35	20.35502759046403	24.529238585622235	36.10018556003818	19.015548263875555
36	21.56964496304255	25.80755147862594	33.72696540490212	18.895838153429395
37	21.044906278598585	26.310558750218956	34.39576836936658	18.248766601815884
38	21.35017642722459	25.454790699329976	34.4975875321639	18.697445341281536
39	21.250792681384702	25.323933872809413	33.45354301648871	19.971730429317176
40	21.77525035617973	25.077488410693725	33.78925587708263	19.358005356043908
41	20.55342040261189	25.882768394657447	33.69717838211505	19.866632820615617
42	20.804924038408398	26.540892992462382	33.32736968411705	19.32681328501216
43	20.286180226475043	25.485889100478747	34.46667647078108	19.761254202265125
44	20.940464359077875	25.159543228182645	33.64612829589192	20.25386411684756
45	20.942337756737437	24.807344468184557	33.2055051663624	21.044812608715606
46	20.77120288053624	25.079455478236273	33.49204133839276	20.657300302834734
47	20.04235752108275	25.950117040518784	34.07110855496408	19.936416883434386
48	19.933794126710996	25.325807270468975	34.49646349356816	20.24393510925187
49	20.683153190536718	24.648667686419458	33.97135012959228	20.69682899345154
50	20.091346869880354	24.43144722779298	34.07045286578324	21.40675303654343
51	19.758069426243864	24.202799043443154	33.760405553125345	22.278725977187637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	644.0
1	1873.0
2	3102.0
3	36721.0
4	70340.0
5	47241.0
6	24142.0
7	23681.5
8	23221.0
9	23331.0
10	23441.0
11	23141.0
12	22841.0
13	21986.0
14	21131.0
15	20237.0
16	19343.0
17	18306.0
18	17269.0
19	16481.5
20	15694.0
21	14862.0
22	14030.0
23	13673.5
24	13317.0
25	13117.0
26	13172.0
27	13427.0
28	13900.5
29	14374.0
30	15316.5
31	16259.0
32	18236.0
33	20213.0
34	22588.5
35	24964.0
36	26663.0
37	28362.0
38	30895.5
39	33429.0
40	34900.5
41	36372.0
42	39208.0
43	42044.0
44	46447.5
45	50851.0
46	55282.5
47	59714.0
48	63973.0
49	68232.0
50	68422.0
51	68612.0
52	63968.0
53	59324.0
54	54312.0
55	49300.0
56	45915.5
57	42531.0
58	39861.5
59	37192.0
60	34736.0
61	32280.0
62	28809.0
63	25338.0
64	21956.5
65	18575.0
66	16331.0
67	14087.0
68	11987.0
69	9887.0
70	8596.5
71	7306.0
72	6439.5
73	5573.0
74	4570.5
75	2999.5
76	2431.0
77	1730.5
78	1030.0
79	778.5
80	527.0
81	355.0
82	183.0
83	135.5
84	88.0
85	70.0
86	52.0
87	28.0
88	4.0
89	7.0
90	10.0
91	7.5
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1067579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.82676413735149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26972707248993	31.901158850812084
2	7.773418148898198	6.502738549099448
3	3.789547728516392	4.755135570836737
4	2.2609288103078757	3.78269344320361
5	1.6863477630919566	3.5267235070198777
6	1.2709580095626103	3.189603653667181
7	1.000842796700436	2.930341091730946
8	0.8910805697868754	2.9816813455881936
9	0.7178194798158342	2.7021659467907915
>10	4.23476750837956	28.616309200033708
>50	0.06843071305909536	1.9980449243043379
>100	0.03319572329238845	2.26830606257148
>500	0.0024840336221656897	0.7124643854354797
>1k	2.2582123837869907E-4	0.10673269992603635
>5k	0.0	0.0
>10k+	2.2582123837869907E-4	4.0259007689800415
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42623	3.9924914221804664	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1130	0.10584696776538317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.810096489346456E-4	0.0	0.0	0.04795898008484618	0.0
2	2.810096489346456E-4	0.0	0.0	0.1602691697757262	0.0
3	2.810096489346456E-4	0.0	0.0	0.27838689221125557	0.0
4	2.810096489346456E-4	0.0	0.0	0.4258232880189663	0.0
5	2.810096489346456E-4	0.0	0.0	0.7994724512190667	0.0
6	2.810096489346456E-4	0.0	0.0	1.5098648437258508	0.0
7	2.810096489346456E-4	0.0	0.0	2.080689110595094	0.0
8	2.810096489346456E-4	0.0	0.0	2.8853134053779628	0.0
9	2.810096489346456E-4	0.0	0.0	3.5239546675234337	0.0
10	2.810096489346456E-4	0.0	0.0	4.264321422583246	0.0
11	2.810096489346456E-4	0.0	0.0	5.310707685332889	0.0
12	2.810096489346456E-4	0.0	0.0	6.130881180690141	0.0
13	2.810096489346456E-4	0.0	0.0	6.500689878688134	0.0
14	2.810096489346456E-4	0.0	0.0	6.695991584697714	0.0
15	2.810096489346456E-4	0.0	0.0	6.855230385760679	0.0
16	3.7467953191286076E-4	0.0	0.0	7.136052694929369	0.0
17	3.7467953191286076E-4	0.0	0.0	7.485535028321089	0.0
18	3.7467953191286076E-4	0.0	0.0	7.837827458202156	0.0
19	3.7467953191286076E-4	0.0	0.0	8.083898240785928	0.0
20	3.7467953191286076E-4	0.0	0.0	8.308893299699601	0.0
21	3.7467953191286076E-4	0.0	0.0	8.580348620570469	0.0
22	3.7467953191286076E-4	0.0	0.0	8.858173493483855	0.0
23	3.7467953191286076E-4	0.0	0.0	9.104712625482517	0.0
24	3.7467953191286076E-4	0.0	0.0	9.299358642311248	0.0
25	3.7467953191286076E-4	0.0	0.0	9.450822843087023	0.0
26	3.7467953191286076E-4	0.0	0.0	9.603317412575556	0.0
27	5.620192978692912E-4	0.0	0.0	9.763305572702349	0.0
28	5.620192978692912E-4	0.0	0.0	9.935096138084395	0.0
29	5.620192978692912E-4	0.0	0.0	10.10388926721114	0.0
30	5.620192978692912E-4	0.0	0.0	10.295631517667545	0.0
31	6.556891808475063E-4	0.0	0.0	10.465174005858115	0.0
32	6.556891808475063E-4	0.0	0.0	10.671435088176144	0.0
33	6.556891808475063E-4	0.0	0.0	10.853529340685794	0.0
34	6.556891808475063E-4	0.0	0.0	11.047144988801765	0.0
35	6.556891808475063E-4	0.0	0.0	11.241228986332628	0.0
36	6.556891808475063E-4	0.0	0.0	11.434563624799663	0.0
37	6.556891808475063E-4	0.0	0.0	11.64513352173469	0.0
38	6.556891808475063E-4	0.0	0.0	11.88605246075466	0.0
39	6.556891808475063E-4	0.0	0.0	12.218674215210303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	20	7.0330594E-4	45.000004	24
TATTACG	20	7.0330594E-4	45.000004	1
CAGTACG	20	7.0330594E-4	45.000004	21
CGTTTTT	27270	0.0	44.257427	1
CGAATAT	175	0.0	39.857143	14
GTTTTTT	31200	0.0	39.728363	2
CGTTCAT	40	3.457917E-7	39.375004	17
TACGAAT	180	0.0	38.750004	12
ACCGGAA	35	6.247872E-6	38.571426	28
GGTATCA	65	9.094947E-12	38.07692	8
TACCGGG	95	0.0	37.894737	3
TGGGCGA	1815	0.0	37.809917	6
CTACGAA	185	0.0	37.7027	11
ACGGTAT	30	1.1398674E-4	37.500004	40
GCGATCT	330	0.0	36.81818	9
GCTACGA	190	0.0	36.710526	10
TTGGGAC	1540	0.0	36.23377	5
ACCCGCT	75	1.8189894E-12	36.0	34
ACGACGG	25	0.0021070412	36.0	25
TTGGGCG	1540	0.0	35.94156	5
>>END_MODULE