##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933668.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79457
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05323634166908	33.0	31.0	34.0	30.0	34.0
2	32.25227481530891	34.0	31.0	34.0	30.0	34.0
3	32.30246548447588	33.0	31.0	34.0	30.0	34.0
4	35.93202612733931	37.0	35.0	37.0	35.0	37.0
5	35.953534616207506	37.0	35.0	37.0	35.0	37.0
6	35.935373850006926	37.0	35.0	37.0	35.0	37.0
7	35.938545376744656	37.0	35.0	37.0	35.0	37.0
8	35.7350264923166	37.0	35.0	37.0	35.0	37.0
9	37.57517902765017	39.0	37.0	39.0	35.0	39.0
10	37.18836603445889	39.0	37.0	39.0	33.0	39.0
11	37.20773500132147	39.0	37.0	39.0	34.0	39.0
12	37.268522597128005	39.0	37.0	39.0	34.0	39.0
13	37.21353688158375	39.0	37.0	39.0	34.0	39.0
14	38.4154825880665	40.0	38.0	41.0	34.0	41.0
15	38.43032080244661	40.0	38.0	41.0	34.0	41.0
16	38.484186415293806	40.0	38.0	41.0	34.0	41.0
17	38.45265992926992	40.0	38.0	41.0	34.0	41.0
18	38.00244157217111	39.0	38.0	40.0	34.0	41.0
19	37.46028669594875	38.0	37.0	40.0	34.0	41.0
20	36.808135217790756	37.0	35.0	40.0	33.0	41.0
21	36.685515436022	37.0	35.0	40.0	33.0	41.0
22	36.719621933876184	37.0	35.0	40.0	33.0	41.0
23	36.68830940005286	37.0	35.0	40.0	33.0	41.0
24	36.55165687101199	37.0	35.0	40.0	33.0	41.0
25	36.41847791887436	37.0	35.0	40.0	33.0	41.0
26	36.35114590281536	37.0	35.0	40.0	33.0	41.0
27	36.275230627886785	37.0	35.0	40.0	32.0	41.0
28	36.120580943151644	37.0	35.0	40.0	32.0	41.0
29	36.00845740463395	37.0	35.0	40.0	31.0	41.0
30	35.77996903985804	37.0	35.0	40.0	31.0	41.0
31	35.25343267427665	37.0	35.0	40.0	29.0	41.0
32	34.633902613992475	37.0	35.0	40.0	23.0	41.0
33	33.94347886278113	37.0	33.0	40.0	18.0	41.0
34	33.29444856966661	37.0	33.0	40.0	15.0	41.0
35	32.84462036069824	36.0	33.0	40.0	12.0	41.0
36	32.54413078772166	36.0	32.0	40.0	10.0	41.0
37	32.18964974766226	36.0	31.0	40.0	10.0	41.0
38	31.89522634884277	35.0	30.0	40.0	10.0	41.0
39	31.851189951797828	35.0	30.0	40.0	10.0	41.0
40	31.67106736977233	35.0	30.0	40.0	10.0	41.0
41	31.571441156852135	35.0	30.0	40.0	8.0	41.0
42	31.344135821891086	35.0	28.0	40.0	8.0	41.0
43	30.94174207432951	35.0	27.0	39.0	8.0	41.0
44	30.66107454346376	35.0	26.0	39.0	8.0	41.0
45	30.832274060183494	35.0	26.0	39.0	8.0	41.0
46	30.729627345608318	35.0	26.0	39.0	7.0	41.0
47	30.510338925456537	35.0	26.0	39.0	7.0	41.0
48	30.425274047598073	35.0	25.0	39.0	7.0	41.0
49	30.427690448922057	35.0	26.0	39.0	7.0	41.0
50	30.33492329184339	35.0	26.0	39.0	7.0	40.0
51	29.436538001686447	35.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	5.0
14	3.0
15	6.0
16	9.0
17	21.0
18	38.0
19	58.0
20	126.0
21	224.0
22	346.0
23	576.0
24	1035.0
25	1893.0
26	2768.0
27	3102.0
28	2994.0
29	2751.0
30	2624.0
31	2685.0
32	2920.0
33	3350.0
34	4531.0
35	6990.0
36	8999.0
37	9541.0
38	13178.0
39	8678.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.393420340561562	4.545854990749714	34.22354229331589	40.83718237537284
2	45.16279245378003	4.89698830814151	33.814516027536904	16.125703210541552
3	16.51081717155191	5.396629623569981	61.457140340058146	16.635412864819966
4	13.169387215726747	4.976276476584819	62.833985677787986	19.02035062990045
5	10.786966535358747	6.103930427778547	63.47206665240319	19.63703638445952
6	14.741306618674251	7.056646991454498	66.64736901720427	11.554677372666976
7	54.30484412952919	2.9890380960771235	37.91610556653284	4.790012207860856
8	52.46485520470191	3.6661338837358572	37.48190845362901	6.387102457933222
9	47.91396604452723	5.531293655687982	39.98892482726506	6.565815472519727
10	23.880841209710912	16.923619064399613	44.60777527467687	14.587764451212607
11	15.430987829895415	15.243465018815208	48.99631247089621	20.329234680393167
12	13.944649307172433	13.000742539990185	54.919012799375764	18.13559535346162
13	17.03688787646148	14.13217211825264	55.557093773990964	13.273846231294915
14	15.203191663415433	15.905458298199026	54.02922335351196	14.86212668487358
15	10.92666473690172	15.42091949104547	56.23544810400594	17.41696766804687
16	10.786966535358747	14.709843059768177	53.1960683136791	21.30712209119398
17	10.995884566495086	15.108800986697208	51.862013416061515	22.033301030746188
18	12.088299331714008	13.8880149011415	54.85734422391986	19.16634154322464
19	12.321129667618964	16.384962935927607	54.67233849755213	16.62156889890129
20	12.863561423159698	18.891979309563663	52.71530513359427	15.529154133682368
21	12.413003259624702	19.93027675346414	52.22447361465951	15.43224637225166
22	10.520155555835231	18.460299281372315	50.92188227594799	20.097662886844457
23	10.847376568458412	19.283385982355234	48.77858464326617	21.090652805920183
24	12.312319871125263	18.706973583195943	48.524359087305086	20.45634745837371
25	11.457769611236266	20.093887259775727	46.8681173464893	21.58022578249871
26	11.74471726845967	20.480259763142328	48.32928502208742	19.445737946310583
27	12.415520344337189	19.849730042664586	49.543778395861914	18.190971217136312
28	11.04748480310105	18.699422329058486	49.746403715217035	20.50668915262343
29	13.281397485432372	17.658607800445523	47.66099903092238	21.39899568319972
30	17.354040550234718	19.433152522748152	45.24459770693583	17.968209220081302
31	16.870760285437406	24.712737707187536	40.48604905798104	17.930452949394013
32	15.313943390764816	25.690625117988347	38.82477314774029	20.17065834350655
33	17.50129000591515	22.340385365669484	37.893451804120474	22.2648728242949
34	18.885686597782446	22.19691153705778	37.257887914217754	21.65951395094202
35	20.623733591754032	22.795977698629446	34.742061744088	21.838226965528527
36	21.907446795121892	24.56422970915086	33.62573467410046	19.902588821626793
37	18.272776470292108	28.339856777879856	34.08510263412915	19.30226411769888
38	16.179820531860003	33.166366714071756	32.72587688938671	17.927935864681526
39	20.22855129189373	29.16294347886278	33.42688498181406	17.18162024742943
40	23.252828573945656	25.498068137483166	34.13418578602263	17.11491750254855
41	22.433517500031463	25.92219691153706	32.2690260140705	19.375259574360975
42	23.386234063707416	25.422555596108587	33.239362170733855	17.951848169450145
43	21.359980870156186	23.25031148923317	36.92814981688209	18.46155782372856
44	18.308015656266914	23.37239009778874	36.4952112463345	21.824382999609853
45	21.213989956832	21.727475238179142	34.74080320173176	22.31773160325711
46	22.751928716160943	22.140277131026846	37.950086210151404	17.15770794266081
47	17.825993933825842	24.783216079137144	40.44200007551254	16.94878991152447
48	17.122468756686008	23.809104295405064	40.877455730772624	18.190971217136312
49	21.263073108725475	18.951130800307084	42.0768465962722	17.708949494695243
50	20.004530752482474	16.9085165561247	39.883207269340645	23.20374542205218
51	17.824735391469602	16.562417408157874	37.65684584114678	27.956001359225745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	472.5
2	625.0
3	5373.0
4	10121.0
5	6170.0
6	2219.0
7	2028.5
8	1838.0
9	1756.5
10	1675.0
11	1574.0
12	1473.0
13	1403.0
14	1333.0
15	1206.5
16	1080.0
17	973.0
18	866.0
19	789.5
20	713.0
21	662.0
22	611.0
23	580.0
24	549.0
25	490.5
26	458.5
27	485.0
28	464.5
29	444.0
30	479.0
31	514.0
32	537.0
33	560.0
34	638.5
35	717.0
36	792.0
37	867.0
38	1017.0
39	1167.0
40	1426.0
41	1685.0
42	2206.0
43	2727.0
44	3239.0
45	3751.0
46	4941.5
47	6132.0
48	7595.5
49	9059.0
50	9231.5
51	9404.0
52	7754.0
53	6104.0
54	4649.5
55	3195.0
56	2606.0
57	2017.0
58	1737.0
59	1457.0
60	1407.5
61	1358.0
62	1245.0
63	1132.0
64	1009.0
65	886.0
66	765.5
67	645.0
68	535.0
69	425.0
70	377.5
71	330.0
72	276.5
73	223.0
74	194.5
75	114.5
76	63.0
77	52.0
78	41.0
79	37.0
80	33.0
81	19.5
82	6.0
83	7.0
84	8.0
85	4.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	79457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.1953635299596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.61196117143797	49.94273632279094
2	10.527722935176046	12.884956643215828
3	3.8047054952286934	6.984910077148647
4	1.5301085883514314	3.7454220521791664
5	0.7815070746956235	2.3912304768616988
6	0.4113195129976966	1.5102508274915991
7	0.2591312931885489	1.1100343582063255
8	0.16452780519907864	0.8054671079955197
9	0.13779203685422836	0.7589010408145286
>10	0.719809147745969	7.542444340964296
>50	0.030848963474827244	1.2107177467057653
>100	0.018509378084896347	2.7020904388537197
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.002056597564988483	8.410838566771965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6683	8.410838566771965	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	380	0.47824609537233975	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	338	0.4253873164101338	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	295	0.3712699950916848	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.31841121612947887	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	232	0.29198182664837585	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	208	0.26177681009854387	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	194	0.24415721711114188	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	142	0.1787130145865059	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	105	0.13214694740551494	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95	0.11956152384308494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012585423562429994	0.0	0.0	0.4266458587663768	0.0
2	0.0012585423562429994	0.0	0.0	1.604641504209824	0.0
3	0.0012585423562429994	0.0	0.0	2.17350264923166	0.0
4	0.0012585423562429994	0.0	0.0	3.0997898234265073	0.0
5	0.0012585423562429994	0.0	0.0	4.982569188366035	0.0
6	0.0012585423562429994	0.0	0.0	6.397170796783166	0.0
7	0.0012585423562429994	0.0	0.0	7.797928439281624	0.0
8	0.0012585423562429994	0.0	0.0	9.887108750645003	0.0
9	0.0012585423562429994	0.0	0.0	10.807103213058635	0.0
10	0.0012585423562429994	0.0	0.0	12.994449828208968	0.0
11	0.0012585423562429994	0.0	0.0	18.217400606617417	0.0
12	0.0012585423562429994	0.0	0.0	23.016222610971973	0.0
13	0.0012585423562429994	0.0	0.0	24.559195539725888	0.0
14	0.0012585423562429994	0.0	0.0	25.076456448141762	0.0
15	0.0012585423562429994	0.0	0.0	25.97757277521175	0.0
16	0.0012585423562429994	0.0	0.0	27.835181293026416	0.0
17	0.0012585423562429994	0.0	0.0	30.240255735806787	0.0
18	0.0012585423562429994	0.0	0.0	32.398655876763534	0.0
19	0.0012585423562429994	0.0	0.0	33.6358030129504	0.0
20	0.0012585423562429994	0.0	0.0	34.74835445586921	0.0
21	0.0012585423562429994	0.0	0.0	35.98046742263111	0.0
22	0.0012585423562429994	0.0	0.0	37.035125917162745	0.0
23	0.0012585423562429994	0.0	0.0	37.839334482802016	0.0
24	0.0012585423562429994	0.0	0.0	38.264721799212154	0.0
25	0.0012585423562429994	0.0	0.0	38.542859659941854	0.0
26	0.0025170847124859987	0.0	0.0	38.76184602992814	0.0
27	0.0025170847124859987	0.0	0.0	38.93426633273343	0.0
28	0.0025170847124859987	0.0	0.0	39.11172080496369	0.0
29	0.0025170847124859987	0.0	0.0	39.272814226562794	0.0
30	0.0025170847124859987	0.0	0.0	39.466629749424214	0.0
31	0.0025170847124859987	0.0	0.0	39.61010357803592	0.0
32	0.0025170847124859987	0.0	0.0	39.769938457278776	0.0
33	0.0025170847124859987	0.0	0.0	39.86936330342198	0.0
34	0.0025170847124859987	0.0	0.0	40.00402733553998	0.0
35	0.0025170847124859987	0.0	0.0	40.15253533357665	0.0
36	0.0025170847124859987	0.0	0.0	40.30356041632581	0.0
37	0.0025170847124859987	0.0	0.0	40.43067319430635	0.0
38	0.0025170847124859987	0.0	0.0	40.556527429930654	0.0
39	0.0025170847124859987	0.0	0.0	40.694967089117384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGGG	35	1.1929478E-7	45.0	3
TGATGGG	20	6.993666E-4	45.0	3
CTGGGTT	20	6.993666E-4	45.0	5
ACTTTTG	20	6.993666E-4	45.0	1
GCTCTTG	20	6.993666E-4	45.0	1
CCGGGAC	20	6.993666E-4	45.0	5
TTTATGG	35	1.1929478E-7	45.0	2
TATGGGC	20	6.993666E-4	45.0	4
ATGGGGC	25	3.858003E-5	44.999996	5
CTTTGCG	25	3.858003E-5	44.999996	1
AGCTGGG	25	3.858003E-5	44.999996	3
CGTTTTT	2235	0.0	44.395973	1
TTTGTTG	60	3.6379788E-12	41.250004	1
TTGTTGG	55	5.820766E-11	40.909092	2
GTTTTTT	2550	0.0	40.5	2
TGTCTTG	45	1.8897481E-8	40.000004	1
CTTGGGC	90	0.0	40.000004	4
TTGGGAC	85	0.0	39.705883	5
TATTGGG	40	3.4043478E-7	39.375	3
TTGGGCT	190	0.0	39.07895	5
>>END_MODULE