##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933667.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80660
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96652615918671	33.0	31.0	34.0	30.0	34.0
2	32.18446565831887	34.0	31.0	34.0	30.0	34.0
3	32.242201834862385	33.0	31.0	34.0	30.0	34.0
4	35.83282915943467	37.0	35.0	37.0	35.0	37.0
5	35.92728737912224	37.0	35.0	37.0	35.0	37.0
6	35.928874287131165	37.0	35.0	37.0	35.0	37.0
7	35.872762211753034	37.0	35.0	37.0	35.0	37.0
8	35.72675427721299	37.0	35.0	37.0	35.0	37.0
9	37.54237540292586	39.0	37.0	39.0	35.0	39.0
10	37.219600793454006	39.0	37.0	39.0	34.0	39.0
11	37.21929085048351	39.0	37.0	39.0	34.0	39.0
12	37.24789238780065	39.0	37.0	39.0	34.0	39.0
13	37.23177535333499	39.0	37.0	39.0	34.0	39.0
14	38.440354574758246	40.0	38.0	41.0	34.0	41.0
15	38.41596826183982	40.0	38.0	41.0	34.0	41.0
16	38.5431564592115	40.0	38.0	41.0	34.0	41.0
17	38.53481279444582	40.0	38.0	41.0	34.0	41.0
18	38.14035457475824	39.0	38.0	40.0	34.0	41.0
19	37.653967270022314	38.0	37.0	40.0	34.0	41.0
20	36.96686089759484	38.0	35.0	40.0	34.0	41.0
21	36.863612695264074	38.0	35.0	40.0	33.0	41.0
22	36.87686585668237	38.0	35.0	40.0	33.0	41.0
23	36.75421522439871	38.0	35.0	40.0	33.0	41.0
24	36.66102157203075	37.0	35.0	40.0	33.0	41.0
25	36.54490453756509	37.0	35.0	40.0	33.0	41.0
26	36.5254401190181	37.0	35.0	40.0	33.0	41.0
27	36.44957847756013	37.0	35.0	40.0	33.0	41.0
28	36.40073146541037	37.0	35.0	40.0	33.0	41.0
29	36.19266055045872	37.0	35.0	40.0	32.0	41.0
30	35.89859905777337	37.0	35.0	40.0	31.0	41.0
31	35.400322340689314	37.0	35.0	40.0	29.0	41.0
32	34.85022315893875	37.0	35.0	40.0	24.0	41.0
33	34.10347136126953	37.0	34.0	40.0	20.0	41.0
34	33.47577485742623	37.0	33.0	40.0	15.0	41.0
35	33.06399702454748	37.0	33.0	40.0	12.0	41.0
36	32.76646417059261	37.0	33.0	40.0	10.0	41.0
37	32.488544507810566	36.0	32.0	40.0	10.0	41.0
38	32.22785767418795	36.0	31.0	40.0	10.0	41.0
39	32.179816513761466	36.0	31.0	40.0	10.0	41.0
40	31.99390032234069	35.0	31.0	40.0	10.0	41.0
41	31.941296801388546	36.0	31.0	40.0	10.0	41.0
42	31.627808083312672	35.0	29.0	40.0	8.0	41.0
43	31.255913711877014	35.0	28.0	40.0	8.0	41.0
44	30.979556161666252	35.0	27.0	39.0	8.0	41.0
45	31.099640466154227	35.0	28.0	39.0	8.0	41.0
46	30.960352095214482	35.0	26.0	39.0	8.0	41.0
47	30.91133151500124	35.0	26.0	39.0	8.0	41.0
48	30.796045127696505	35.0	26.0	39.0	8.0	41.0
49	30.790614926853458	35.0	27.0	39.0	8.0	41.0
50	30.592251425737665	35.0	26.0	39.0	8.0	41.0
51	29.801078601537316	35.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	2.0
12	1.0
13	1.0
14	3.0
15	9.0
16	11.0
17	26.0
18	39.0
19	90.0
20	131.0
21	213.0
22	347.0
23	553.0
24	987.0
25	1746.0
26	2812.0
27	3114.0
28	2959.0
29	2693.0
30	2650.0
31	2521.0
32	2818.0
33	3279.0
34	4552.0
35	6727.0
36	8876.0
37	8938.0
38	14089.0
39	10466.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.25291346392264	4.889660302504339	33.69452020828167	41.16290602529135
2	44.871063724274734	4.89337961815026	34.18918918918919	16.046367468385817
3	16.82494421026531	5.5504587155963305	61.14554921894372	16.479047855194644
4	13.52963054797917	5.131415819489214	62.108851971237286	19.23010166129432
5	10.975700471113315	6.22737416315398	63.10190924869824	19.695016117034466
6	14.787999008182496	7.076617902305976	66.47904785519464	11.656335234316886
7	54.26977436151748	3.250681874535086	37.59980163649889	4.87974212744855
8	52.81552194396231	3.5841805107860156	37.25142573766427	6.348871807587404
9	48.19985122737416	5.585172328291595	39.66402181998512	6.55095462434912
10	24.101165385569058	17.33077113811059	44.181750557897345	14.38631291842301
11	15.829407389040417	15.050830647160923	48.99206545995537	20.127696503843293
12	14.024299528886687	13.039920654599554	54.28961071162905	18.646169104884702
13	17.00843044879742	14.21274485494669	55.63724274733449	13.141581948921399
14	15.399206545995536	15.929828911480287	53.77138606496404	14.89957847756013
15	11.212496900570295	15.42400198363501	56.10587651872056	17.25762459707414
16	11.027770890156212	14.568559385073145	52.840317381601785	21.563352343168855
17	11.108356062484503	15.26655095462435	51.43565583932557	22.189437143565584
18	12.04066451772874	14.101165385569056	54.48797421274485	19.370195883957354
19	12.570047111331514	16.518720555417804	54.49417307215473	16.41705926109596
20	13.137862633275477	18.668484998760228	52.12372923382098	16.06992313414332
21	12.470865360773619	20.3396974956608	51.90428961071163	15.285147532853955
22	10.994297049342922	18.26927845276469	50.72526655095463	20.011157946937765
23	11.125712868832135	19.505331019092488	48.43044879742128	20.938507314654103
24	12.46466650136375	18.760228118026284	48.22588643689561	20.549218943714358
25	11.522439871063725	20.454996280684355	46.29184230101661	21.73072154723531
26	11.728242003471362	20.502107612199357	47.98289114802876	19.786759236300522
27	12.860153731713364	19.98264319365237	49.17307215472353	17.984130919910736
28	11.191420778576742	18.63625092982891	49.61443094470617	20.557897346888172
29	13.327547731217457	17.845276469129683	47.27126208777585	21.555913711877015
30	17.509298289114803	19.335482271262087	45.1698487478304	17.98537069179271
31	17.29853706917927	24.6131911728242	40.278948673444084	17.809323084552442
32	15.487230349615672	25.83684602033226	38.720555417803126	19.95536821224895
33	17.650632283659807	22.1981155467394	37.658070914951644	22.493181254649144
34	19.216464170592612	22.21795189685098	37.18075874039177	21.38482519216464
35	20.601289362757253	22.971733201091	34.42226630300025	22.0047111331515
36	22.190676915447558	24.7359285891396	33.38829655343417	19.685097941978675
37	18.363501115794694	28.082072898586656	33.8866848499876	19.667741135631044
38	16.35755021076122	33.16885693032482	32.53161418299033	17.941978675923632
39	20.261591867096453	29.464418546987353	32.980411604264816	17.293577981651374
40	22.971733201091	25.87403917679147	34.403669724770644	16.750557897346887
41	22.733696999752045	25.80461195140094	32.06050086784032	19.401190181006694
42	23.316389784279693	25.45375650880238	33.18993305231837	18.039920654599552
43	21.55963302752294	22.873791222415075	37.04934292090255	18.517232829159436
44	18.426729481775354	23.15150012397719	36.839821472848996	21.581948921398464
45	21.148028762707664	21.338953632531616	35.09670220679395	22.416315397966773
46	22.872551450533102	21.872055541780313	38.09695016117035	17.15844284651624
47	17.80436399702455	24.817753533349865	40.74882221671213	16.62906025291346
48	17.177039424745846	24.192908504835113	40.84552442350608	17.784527646912967
49	21.16414579717332	19.124721051326556	42.343168856930326	17.3679642945698
50	20.249194148276718	16.76171584428465	40.012397718819734	22.976692288618892
51	17.93825936027771	16.51500123977188	37.86883213488718	27.67790726506323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	269.0
1	438.5
2	608.0
3	5444.5
4	10281.0
5	6199.5
6	2118.0
7	1999.0
8	1880.0
9	1804.5
10	1729.0
11	1608.0
12	1487.0
13	1409.5
14	1332.0
15	1203.5
16	1075.0
17	973.5
18	872.0
19	786.5
20	701.0
21	639.5
22	578.0
23	573.5
24	569.0
25	505.0
26	433.5
27	426.0
28	457.5
29	489.0
30	509.0
31	529.0
32	557.5
33	586.0
34	667.0
35	748.0
36	844.5
37	941.0
38	1082.5
39	1224.0
40	1514.5
41	1805.0
42	2309.5
43	2814.0
44	3382.0
45	3950.0
46	5041.0
47	6132.0
48	7637.5
49	9143.0
50	9239.5
51	9336.0
52	7801.5
53	6267.0
54	4769.0
55	3271.0
56	2644.5
57	2018.0
58	1772.5
59	1527.0
60	1474.0
61	1421.0
62	1285.5
63	1150.0
64	1034.5
65	919.0
66	791.5
67	664.0
68	577.0
69	490.0
70	413.0
71	336.0
72	279.5
73	223.0
74	197.5
75	114.0
76	56.0
77	49.0
78	42.0
79	32.0
80	22.0
81	18.0
82	14.0
83	9.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	80660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.36994792958096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.73572251065634	50.16117034465658
2	10.409890709278601	12.777089015621126
3	3.7090159794751623	6.8286635259112325
4	1.6868346094018303	4.140838085792215
5	0.713116906729157	2.1881973716836103
6	0.40605240298175793	1.4951648896603027
7	0.23231853901941377	0.998016364988842
8	0.19797579846871782	0.971981155467394
9	0.15757257429142846	0.8703198611455493
>10	0.7030161006848347	7.462186957599802
>50	0.028282256924102543	1.1418299032977932
>100	0.01818145087978021	2.6072402677907265
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0020201612088644673	8.357302256384825
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6741	8.357302256384825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	373	0.4624349119761964	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	339	0.42028266798909003	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	311	0.38556905529382596	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	216	0.26779072650632285	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.25167369204066453	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	203	0.25167369204066453	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	195	0.2417555169848748	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.19588395735184727	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	105	0.13017604760724028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	91	0.11281924125960824	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTG	88	0.10909992561368709	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001239771881973717	0.0	0.0	0.4240019836350112	0.0
2	0.001239771881973717	0.0	0.0	1.5137614678899083	0.0
3	0.001239771881973717	0.0	0.0	2.139846268286635	0.0
4	0.001239771881973717	0.0	0.0	3.0262831638978427	0.0
5	0.001239771881973717	0.0	0.0	4.853706917927101	0.0
6	0.001239771881973717	0.0	0.0	6.245970741383585	0.0
7	0.001239771881973717	0.0	0.0	7.547731217455988	0.0
8	0.001239771881973717	0.0	0.0	9.656583188693281	0.0
9	0.001239771881973717	0.0	0.0	10.539300768658567	0.0
10	0.001239771881973717	0.0	0.0	12.609719811554674	0.0
11	0.001239771881973717	0.0	0.0	17.656831143069674	0.0
12	0.001239771881973717	0.0	0.0	22.61467889908257	0.0
13	0.001239771881973717	0.0	0.0	24.16935283907761	0.0
14	0.001239771881973717	0.0	0.0	24.708653607736178	0.0
15	0.001239771881973717	0.0	0.0	25.529382593602776	0.0
16	0.001239771881973717	0.0	0.0	27.364244978923878	0.0
17	0.001239771881973717	0.0	0.0	29.65906273245723	0.0
18	0.001239771881973717	0.0	0.0	31.713364740887677	0.0
19	0.001239771881973717	0.0	0.0	32.91594346640218	0.0
20	0.001239771881973717	0.0	0.0	34.011901810066945	0.0
21	0.001239771881973717	0.0	0.0	35.23803620133895	0.0
22	0.001239771881973717	0.0	0.0	36.262087775849245	0.0
23	0.001239771881973717	0.0	0.0	36.9761963798661	0.0
24	0.001239771881973717	0.0	0.0	37.359285891395984	0.0
25	0.001239771881973717	0.0	0.0	37.65311182742376	0.0
26	0.001239771881973717	0.0	0.0	37.86511281924126	0.0
27	0.001239771881973717	0.0	0.0	38.02132407636995	0.0
28	0.001239771881973717	0.0	0.0	38.196131911728244	0.0
29	0.001239771881973717	0.0	0.0	38.367220431440614	0.0
30	0.001239771881973717	0.0	0.0	38.55566575750062	0.0
31	0.001239771881973717	0.0	0.0	38.708157698983385	0.0
32	0.001239771881973717	0.0	0.0	38.8395735184726	0.0
33	0.001239771881973717	0.0	0.0	38.96355070666997	0.0
34	0.001239771881973717	0.0	0.0	39.099925613687084	0.0
35	0.001239771881973717	0.0	0.0	39.211505083064715	0.0
36	0.001239771881973717	0.0	0.0	39.336722043144064	0.0
37	0.001239771881973717	0.0	0.0	39.4668980907513	0.0
38	0.001239771881973717	0.0	0.0	39.59211505083065	0.0
39	0.001239771881973717	0.0	0.0	39.76072402677907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGGA	30	2.1404048E-6	45.000004	4
TGGGCGA	70	0.0	45.000004	6
TGTCGGG	30	2.1404048E-6	45.000004	3
TTGTCGG	25	3.8585255E-5	45.0	2
GAGAACC	20	6.994298E-4	45.0	7
TGCATGG	20	6.994298E-4	45.0	2
CATTGGG	20	6.994298E-4	45.0	3
CTGAGAA	20	6.994298E-4	45.0	5
ACATGGG	20	6.994298E-4	45.0	3
CTGGGCG	25	3.8585255E-5	45.0	5
CGGGGAC	20	6.994298E-4	45.0	6
GCGCGAC	25	3.8585255E-5	45.0	9
TCAGACG	20	6.994298E-4	45.0	22
GGGACCC	20	6.994298E-4	45.0	7
TGGGCTC	20	6.994298E-4	45.0	6
TGTGGGC	20	6.994298E-4	45.0	4
TGGGCGC	20	6.994298E-4	45.0	6
GGGGTAC	20	6.994298E-4	45.0	7
GGGAAGA	25	3.8585255E-5	45.0	7
GGGCGCG	25	3.8585255E-5	45.0	7
>>END_MODULE