SRR2933666.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933666.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166010
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4726	2.8468164568399494	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	847	0.5102102282995	Illumina PCR Primer Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	813	0.4897295343653997	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	805	0.48491054755737606	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	566	0.34094331666767064	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	544	0.3276911029456057	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	399	0.2403469670501777	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGT	245	0.14758147099572314	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	244	0.1469790976447202	Illumina PCR Primer Index 4 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	224	0.13493163062466115	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.1331245105716523	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	201	0.12107704355159327	TruSeq Adapter, Index 13 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	191	0.11505331004156376	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.10119872296849587	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGGG	30	2.1542546E-6	45.000004	3
ACTGCGG	25	3.8763494E-5	45.000004	2
CAGCATA	30	2.1542546E-6	45.000004	38
TTCGTTG	25	3.8763494E-5	45.000004	1
ATAGAGA	30	2.1542546E-6	45.000004	42
CGGGGTA	25	3.8763494E-5	45.000004	6
CGGGCTC	25	3.8763494E-5	45.000004	6
CTTTGCG	25	3.8763494E-5	45.000004	1
GCGATTC	25	3.8763494E-5	45.000004	9
CAGTGCC	30	2.1542546E-6	45.000004	19
GCTGTTG	25	3.8763494E-5	45.000004	1
ACTCAGT	30	2.1542546E-6	45.000004	16
AGGGCAC	30	2.1542546E-6	45.000004	11
TTGGGAC	205	0.0	45.000004	5
TATGGGA	55	1.8189894E-12	45.0	4
AGGGTGG	20	7.0158386E-4	45.0	6
CTACGAA	20	7.0158386E-4	45.0	11
TACGAAT	20	7.0158386E-4	45.0	12
TTGGTTG	40	6.7539077E-9	45.0	1
AGTTATA	20	7.0158386E-4	45.0	20