##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933666.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166010
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83476296608638	33.0	31.0	34.0	30.0	34.0
2	32.00425275585808	33.0	31.0	34.0	30.0	34.0
3	32.154087103186555	33.0	31.0	34.0	30.0	34.0
4	35.75916511053551	37.0	35.0	37.0	35.0	37.0
5	35.77509788566954	37.0	35.0	37.0	35.0	37.0
6	35.72595626769472	37.0	35.0	37.0	35.0	37.0
7	35.78377808565749	37.0	35.0	37.0	35.0	37.0
8	35.54831034275043	37.0	35.0	37.0	33.0	37.0
9	37.33416059273538	39.0	37.0	39.0	34.0	39.0
10	36.85283416661647	39.0	37.0	39.0	32.0	39.0
11	36.87972411300524	39.0	37.0	39.0	33.0	39.0
12	37.02635985783989	39.0	37.0	39.0	33.0	39.0
13	36.96843563640744	39.0	37.0	39.0	33.0	39.0
14	37.96664056382146	40.0	37.0	41.0	33.0	41.0
15	37.96149027167038	39.0	37.0	41.0	33.0	41.0
16	38.077164026263475	40.0	37.0	41.0	33.0	41.0
17	38.074134088307936	40.0	37.0	41.0	33.0	41.0
18	37.913372688392265	39.0	37.0	40.0	33.0	41.0
19	37.69416902596229	39.0	37.0	40.0	33.0	41.0
20	37.339280766218906	39.0	35.0	40.0	33.0	41.0
21	37.21475212336606	39.0	35.0	40.0	33.0	41.0
22	37.23558821757725	39.0	35.0	40.0	33.0	41.0
23	37.189464490090955	39.0	35.0	40.0	33.0	41.0
24	37.050406602011925	39.0	35.0	40.0	32.0	41.0
25	36.972519727727246	38.0	35.0	40.0	32.0	41.0
26	36.90562616709837	38.0	35.0	40.0	32.0	41.0
27	36.83464851514969	38.0	35.0	40.0	32.0	41.0
28	36.75759291608939	38.0	35.0	40.0	32.0	41.0
29	36.67644117824227	38.0	35.0	40.0	31.0	41.0
30	36.58189868080236	38.0	35.0	40.0	31.0	41.0
31	36.34519607252575	38.0	35.0	40.0	31.0	41.0
32	35.991175230407805	38.0	35.0	40.0	30.0	41.0
33	35.74418408529607	38.0	35.0	40.0	28.0	41.0
34	35.535521956508646	38.0	35.0	40.0	26.0	41.0
35	35.25421962532378	38.0	35.0	40.0	24.0	41.0
36	35.12548039274743	38.0	35.0	40.0	24.0	41.0
37	35.04629841575809	38.0	35.0	40.0	23.0	41.0
38	34.85764110595747	38.0	34.0	40.0	23.0	41.0
39	34.68665140654178	38.0	34.0	40.0	22.0	41.0
40	34.53408830793326	38.0	34.0	40.0	21.0	41.0
41	34.43088368170592	38.0	34.0	40.0	21.0	41.0
42	34.337449551231856	38.0	33.0	40.0	20.0	41.0
43	34.2657068851274	38.0	33.0	40.0	19.0	41.0
44	34.17631467983856	38.0	33.0	40.0	18.0	41.0
45	34.109294620805976	38.0	33.0	40.0	20.0	41.0
46	33.70191554725619	37.0	33.0	40.0	18.0	41.0
47	33.42969098247094	37.0	33.0	40.0	17.0	41.0
48	33.400680681886634	37.0	33.0	40.0	17.0	41.0
49	33.520197578459126	37.0	33.0	40.0	17.0	41.0
50	33.456767664598516	37.0	33.0	40.0	15.0	41.0
51	32.2207216432745	36.0	30.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	3.0
13	8.0
14	6.0
15	5.0
16	17.0
17	14.0
18	59.0
19	101.0
20	179.0
21	324.0
22	483.0
23	757.0
24	1241.0
25	2135.0
26	3141.0
27	3683.0
28	3736.0
29	3759.0
30	3897.0
31	4577.0
32	5416.0
33	7064.0
34	10029.0
35	13171.0
36	17475.0
37	23341.0
38	36255.0
39	25125.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.173784711764352	5.7629058490452385	41.36859225347871	27.694717185711703
2	33.4895488223601	5.715920727667008	41.91253538943437	18.88199506053852
3	21.002951629419915	6.070718631407747	51.89145232214927	21.034877417023072
4	16.486356243599783	6.127341726402024	53.196795373772666	24.18950665622553
5	13.717848322390216	7.015842419131378	54.018432624540694	25.247876633937715
6	18.26938136256852	8.049515089452443	58.78079633757003	14.90030721040901
7	65.99000060237336	3.5431600505993615	24.186494789470515	6.280344557556774
8	63.672067947714	4.3629901813143785	23.72628154930426	8.23866032166737
9	57.102584181675795	6.838142280585507	27.0610204204566	8.998253117282092
10	28.79404855129209	20.67887476658033	32.54683452804048	17.980242154087104
11	18.9392205288838	19.09764472019758	37.29715077404976	24.665983976868862
12	18.321185470754774	14.605746641768569	44.24793687127281	22.82513101620384
13	21.167399554243723	16.121317992891996	46.1020420456599	16.609240407204386
14	18.339859044635865	19.350039154267815	43.19498825371966	19.115113547376662
15	12.85946629721101	18.90548762122764	46.097825432202875	22.137220649358472
16	12.029998192879948	17.67785073188362	43.95699054273839	26.33516053249804
17	11.670381302331185	18.222998614541293	41.83603397385699	28.270586109270525
18	13.204023853984701	17.53870248780194	44.754532859466295	24.502740798747062
19	14.01180651767966	20.578278416962835	45.70748750075297	19.70242756460454
20	14.812963074513583	23.46726100837299	42.51189687368231	19.20787904343112
21	13.7148364556352	25.492440214444912	41.80772242635985	18.985000903560024
22	11.122823926269502	23.810011445093668	39.87048972953436	25.19667489910246
23	12.13059454249744	24.36479730136739	36.96162881754111	26.54297933859406
24	13.694355761701102	23.1016203843142	37.0604180471056	26.143605806879105
25	12.976929100656587	25.396662851635444	34.01843262454069	27.60797542316728
26	11.823986506836938	25.905065959881934	37.027287512800434	25.243660020480696
27	13.960002409493406	24.59610866815252	39.047647732064334	22.396241190289743
28	11.572796819468707	21.790855972531777	39.033793144991265	27.60255406300825
29	13.416059273537739	20.782482982952835	36.856213481115596	28.94524426239383
30	19.00427685079212	22.3667248960906	35.1418589241612	23.487139328956086
31	17.813987109210288	27.829046442985362	29.156074935244863	25.200891512559487
32	14.745497259201255	28.4494909945184	28.58743449189808	28.217577254382263
33	18.033853382326363	23.907595927956145	28.774170230708997	29.284380459008492
34	18.780193964219023	20.87585085235829	28.561532437804953	31.782422745617733
35	16.329739172339018	20.895729172941387	29.36088187458587	33.413649780133724
36	15.722546834528039	22.237816998975966	32.73778688030842	29.301849286187583
37	16.117703752785978	23.123305824950304	33.10583699777122	27.653153424492498
38	15.279802421540872	28.36154448527197	28.655502680561412	27.703150412625742
39	19.166315282211915	29.283175712306488	26.337570025902053	25.21293897957954
40	23.953978675983375	23.829287392325764	30.251189687368228	21.965544244322633
41	20.490331907716403	19.541593879886754	33.65700861393892	26.311065598457922
42	17.179085597253177	18.097704957532677	35.15209927112824	29.571110174085895
43	16.047828444069633	19.02234805132221	35.44003373290766	29.489789771700497
44	17.15318354316005	21.514968977772423	31.192096861634845	30.139750617432686
45	22.62032407686284	19.76326727305584	29.086199626528526	28.5302090235528
46	23.908800674658153	20.147581470995725	33.79977109812662	22.143846756219503
47	17.037527859767483	22.703451599301246	37.45798445876755	22.801036082163726
48	15.653876272513703	21.675802662490213	38.16878501295103	24.501536052045058
49	21.091500512017348	17.124871995662915	37.50015059333775	24.28347689898199
50	21.355942413107645	14.78404915366544	34.0202397445937	29.839768688633217
51	18.176615866514066	14.219022950424673	31.814348533220894	35.79001264984037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	411.0
1	714.5
2	1018.0
3	4365.0
4	7712.0
5	4879.5
6	2047.0
7	2013.0
8	1979.0
9	1947.5
10	1916.0
11	1770.5
12	1625.0
13	1544.0
14	1463.0
15	1318.0
16	1173.0
17	1069.0
18	965.0
19	955.0
20	945.0
21	869.0
22	793.0
23	774.5
24	756.0
25	782.5
26	838.5
27	868.0
28	864.0
29	860.0
30	984.5
31	1109.0
32	1176.5
33	1244.0
34	1461.5
35	1679.0
36	1807.5
37	1936.0
38	2258.0
39	2580.0
40	3496.5
41	4413.0
42	5587.5
43	6762.0
44	8493.0
45	10224.0
46	13433.5
47	16643.0
48	20649.5
49	24656.0
50	24854.5
51	25053.0
52	20919.5
53	16786.0
54	12513.0
55	8240.0
56	6434.0
57	4628.0
58	4083.5
59	3539.0
60	3306.0
61	3073.0
62	2787.5
63	2502.0
64	2186.5
65	1871.0
66	1531.5
67	1192.0
68	1012.5
69	833.0
70	713.5
71	594.0
72	532.5
73	471.0
74	367.0
75	221.5
76	180.0
77	142.5
78	105.0
79	79.5
80	54.0
81	35.5
82	17.0
83	17.0
84	17.0
85	10.0
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	166010.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.28456117101379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20199490571288	43.452804047948916
2	12.188831096556004	13.720860189145231
3	4.649072111988698	7.850129510270467
4	2.167212483143903	4.879224143123908
5	1.105545923500075	3.1112583579302453
6	0.6153813223741946	2.078188060960183
7	0.40133564502664865	1.5812300463827482
8	0.26755709668443245	1.2047467020059033
9	0.22367773282818554	1.1330642732365521
>10	1.0787902138316317	10.856574905126196
>50	0.06635415997773925	2.601650502981748
>100	0.027825938055180972	2.529968074212397
>500	0.005351141933688649	2.153484729835552
>1k	0.0010702283867377298	2.8468164568399494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4726	2.8468164568399494	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	847	0.5102102282995	Illumina PCR Primer Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	813	0.4897295343653997	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	805	0.48491054755737606	TruSeq Adapter, Index 13 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	566	0.34094331666767064	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	544	0.3276911029456057	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	399	0.2403469670501777	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGT	245	0.14758147099572314	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	244	0.1469790976447202	Illumina PCR Primer Index 4 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	224	0.13493163062466115	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.1331245105716523	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	201	0.12107704355159327	TruSeq Adapter, Index 13 (95% over 24bp)
GGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	191	0.11505331004156376	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.10119872296849587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5879163905788808	0.0
2	0.0	0.0	0.0	1.9607252575146075	0.0
3	0.0	0.0	0.0	2.8160954159387988	0.0
4	0.0	0.0	0.0	4.098548280224083	0.0
5	0.0	0.0	0.0	6.735738810915005	0.0
6	0.0	0.0	0.0	9.039214505150293	0.0
7	0.0	0.0	0.0	11.121016806216494	0.0
8	0.0	0.0	0.0	14.122643214264201	0.0
9	0.0	0.0	0.0	15.558701283055237	0.0
10	0.0	0.0	0.0	18.798867538100115	0.0
11	0.0	0.0	0.0	26.285163544364796	0.0
12	0.0	0.0	0.0	33.2449852418529	0.0
13	0.0	0.0	0.0	35.570748750075296	0.0
14	0.0	0.0	0.0	36.347810372869105	0.0
15	0.0	0.0	0.0	37.46762243238359	0.0
16	0.0	0.0	0.0	40.133726883922655	0.0
17	0.0	0.0	0.0	43.341967351364374	0.0
18	0.0	0.0	0.0	46.24058791639058	0.0
19	0.0	0.0	0.0	47.929642792602856	0.0
20	0.0	0.0	0.0	49.358472381181855	0.0
21	0.0	0.0	0.0	51.05716523101018	0.0
22	0.0	0.0	0.0	52.47695921932414	0.0
23	0.0	0.0	0.0	53.53653394373833	0.0
24	0.0	0.0	0.0	54.14011204144329	0.0
25	0.0	0.0	0.0	54.50635503885308	0.0
26	0.0	0.0	0.0	54.76537557978435	0.0
27	0.0	0.0	0.0	54.9996988133245	0.0
28	0.0	0.0	0.0	55.205108126016505	0.0
29	0.0	0.0	0.0	55.432202879344615	0.0
30	0.0	0.0	0.0	55.66230949942774	0.0
31	0.0	0.0	0.0	55.85265947834468	0.0
32	0.0	0.0	0.0	56.03397385699657	0.0
33	0.0	0.0	0.0	56.2038431419794	0.0
34	0.0	0.0	0.0	56.36106258659117	0.0
35	0.0	0.0	0.0	56.52671525811698	0.0
36	0.0	0.0	0.0	56.70682489006686	0.0
37	0.0	0.0	0.0	56.87428468164568	0.0
38	0.0	0.0	0.0	57.01463767242937	0.0
39	6.023733510029517E-4	0.0	0.0	57.197156797783265	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGGG	30	2.1542546E-6	45.000004	3
ACTGCGG	25	3.8763494E-5	45.000004	2
CAGCATA	30	2.1542546E-6	45.000004	38
TTCGTTG	25	3.8763494E-5	45.000004	1
ATAGAGA	30	2.1542546E-6	45.000004	42
CGGGGTA	25	3.8763494E-5	45.000004	6
CGGGCTC	25	3.8763494E-5	45.000004	6
CTTTGCG	25	3.8763494E-5	45.000004	1
GCGATTC	25	3.8763494E-5	45.000004	9
CAGTGCC	30	2.1542546E-6	45.000004	19
GCTGTTG	25	3.8763494E-5	45.000004	1
ACTCAGT	30	2.1542546E-6	45.000004	16
AGGGCAC	30	2.1542546E-6	45.000004	11
TTGGGAC	205	0.0	45.000004	5
TATGGGA	55	1.8189894E-12	45.0	4
AGGGTGG	20	7.0158386E-4	45.0	6
CTACGAA	20	7.0158386E-4	45.0	11
TACGAAT	20	7.0158386E-4	45.0	12
TTGGTTG	40	6.7539077E-9	45.0	1
AGTTATA	20	7.0158386E-4	45.0	20
>>END_MODULE