SRR2933665.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933665.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167953
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4614	2.7471971325311246	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	814	0.48465939876036745	Illumina PCR Primer Index 4 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	798	0.47513292409185903	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	795	0.47334671009151374	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	603	0.3590290140694123	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	584	0.34771632540055847	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	412	0.24530672271409265	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	266	0.158377641363953	Illumina PCR Primer Index 4 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.14468333402797212	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGT	232	0.13813388269337254	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	203	0.12086714735670097	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189	0.11253148202175609	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	183	0.10895905402106541	TruSeq Adapter, Index 13 (95% over 24bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.10776824468750187	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	180	0.10717284002072008	Illumina PCR Primer Index 4 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTT	20	7.0160744E-4	45.000004	9
TACCATT	20	7.0160744E-4	45.000004	4
GATAACG	20	7.0160744E-4	45.000004	17
TTGCTAG	20	7.0160744E-4	45.000004	1
TTTCGCG	20	7.0160744E-4	45.000004	1
TCGGGAC	20	7.0160744E-4	45.000004	5
TAACGAA	20	7.0160744E-4	45.000004	19
GAAAGGT	20	7.0160744E-4	45.000004	9
CTAGTTA	20	7.0160744E-4	45.000004	45
AACGAGA	20	7.0160744E-4	45.000004	24
CTACCAT	20	7.0160744E-4	45.000004	3
GTTTTCG	20	7.0160744E-4	45.000004	1
TGCTAAC	20	7.0160744E-4	45.000004	39
ATAACGA	20	7.0160744E-4	45.000004	18
GCTACCA	20	7.0160744E-4	45.000004	2
AGCTACC	20	7.0160744E-4	45.000004	1
CGATAAC	20	7.0160744E-4	45.000004	16
GCGTAGG	20	7.0160744E-4	45.000004	2
TAACTAG	20	7.0160744E-4	45.000004	42
TTGATCC	25	3.876546E-5	45.0	17