##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933665.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167953
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752817752585546	33.0	31.0	34.0	30.0	34.0
2	31.93451739474734	33.0	31.0	34.0	30.0	34.0
3	32.099771960012625	33.0	31.0	34.0	30.0	34.0
4	35.662441278214736	37.0	35.0	37.0	33.0	37.0
5	35.75075169839181	37.0	35.0	37.0	35.0	37.0
6	35.73530094728883	37.0	35.0	37.0	35.0	37.0
7	35.70691800682334	37.0	35.0	37.0	35.0	37.0
8	35.520240781647246	37.0	35.0	37.0	33.0	37.0
9	37.32009550290855	39.0	37.0	39.0	34.0	39.0
10	36.88020458104351	39.0	37.0	39.0	32.0	39.0
11	36.89880502283377	39.0	37.0	39.0	33.0	39.0
12	36.98633546289736	39.0	37.0	39.0	33.0	39.0
13	36.968645990247275	39.0	37.0	39.0	33.0	39.0
14	38.001131268866885	40.0	37.0	41.0	33.0	41.0
15	37.98977690187136	40.0	37.0	41.0	33.0	41.0
16	38.15110179633588	40.0	37.0	41.0	33.0	41.0
17	38.207665239680146	40.0	37.0	41.0	33.0	41.0
18	38.09147797300435	39.0	37.0	41.0	34.0	41.0
19	37.9425732198889	39.0	37.0	41.0	34.0	41.0
20	37.52591498812168	39.0	35.0	40.0	33.0	41.0
21	37.44161759539871	39.0	35.0	40.0	33.0	41.0
22	37.43971825451168	39.0	35.0	40.0	33.0	41.0
23	37.343078123046325	39.0	35.0	40.0	33.0	41.0
24	37.22359826856323	39.0	35.0	40.0	33.0	41.0
25	37.1575917072038	39.0	35.0	40.0	33.0	41.0
26	37.14852369412871	39.0	35.0	40.0	33.0	41.0
27	37.09644364792531	39.0	35.0	40.0	33.0	41.0
28	37.078914934535256	39.0	35.0	40.0	33.0	41.0
29	36.92433597494537	39.0	35.0	40.0	32.0	41.0
30	36.73700975868249	39.0	35.0	40.0	31.0	41.0
31	36.48265883907998	38.0	35.0	40.0	31.0	41.0
32	36.24953409584824	39.0	35.0	40.0	30.0	41.0
33	35.89968622174061	39.0	35.0	40.0	29.0	41.0
34	35.67542109995058	39.0	35.0	40.0	27.0	41.0
35	35.4589557792954	39.0	35.0	40.0	25.0	41.0
36	35.31727328478801	38.0	35.0	40.0	24.0	41.0
37	35.2774228504403	38.0	35.0	40.0	24.0	41.0
38	35.0548189076706	38.0	35.0	40.0	23.0	41.0
39	34.976719677528834	38.0	35.0	40.0	23.0	41.0
40	34.81010163557662	38.0	34.0	40.0	23.0	41.0
41	34.704768595976255	38.0	34.0	40.0	22.0	41.0
42	34.54196709793811	38.0	34.0	40.0	21.0	41.0
43	34.52836210130215	38.0	34.0	40.0	21.0	41.0
44	34.45713384101504	38.0	34.0	40.0	20.0	41.0
45	34.405559888778406	38.0	34.0	40.0	20.0	41.0
46	34.02570361946497	38.0	33.0	40.0	19.0	41.0
47	33.89240442266586	37.0	33.0	40.0	19.0	41.0
48	33.81962215619846	37.0	33.0	40.0	18.0	41.0
49	33.91512506475026	37.0	33.0	40.0	18.0	41.0
50	33.75067429578513	37.0	33.0	40.0	18.0	41.0
51	32.711591933457576	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	4.0
13	3.0
14	7.0
15	13.0
16	21.0
17	35.0
18	52.0
19	88.0
20	170.0
21	265.0
22	461.0
23	723.0
24	1215.0
25	2066.0
26	3139.0
27	3629.0
28	3722.0
29	3571.0
30	3743.0
31	4376.0
32	5276.0
33	6600.0
34	9787.0
35	12730.0
36	16460.0
37	22023.0
38	36876.0
39	30882.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.392222824242495	5.768280411781868	41.377647317999674	27.461849445975954
2	33.394461545789596	5.668252427762529	41.939709323441676	18.997576703006196
3	21.346448113460315	6.115401332515645	51.77043577667562	20.767714777348427
4	16.705268735896354	6.271992759879252	52.71712919685865	24.30560930736575
5	13.655606032640083	6.949563270676916	54.02940108244568	25.365429614237318
6	18.590915315594245	8.295773222270517	58.38895405262187	14.724357409513376
7	66.27092103147905	3.5343221020166355	23.929313557959667	6.265443308544652
8	63.87024941501491	4.288104410162367	23.629825010568435	8.211821164254285
9	57.46726762844367	6.734026781301912	26.848582639190727	8.95012295106369
10	29.1039755169601	20.497401058629496	32.59066524563419	17.807958178776207
11	19.11606223169577	19.149404893035552	36.990110328484754	24.744422546783923
12	18.431346864896728	14.64219156549749	44.26238292855739	22.664078641048388
13	21.488154424154377	16.228349597804147	45.73362786017517	16.549868117866307
14	18.503986234244106	19.469732603764147	42.98940775097795	19.036873411013797
15	12.904205343161479	18.91838788232422	46.0432382869017	22.13416848761261
16	12.208772692360363	17.647198918745126	43.86227099248004	26.281757396414474
17	11.755074336272648	18.21819199418885	41.73667633206909	28.29005733746941
18	13.275737855233311	17.644817300077996	44.591641709287714	24.487803135400977
19	14.077747941388363	20.726036450673703	45.47998547212613	19.716230135811806
20	14.899406381547218	23.62148934523349	42.17846659482117	19.30063767839812
21	14.000345334706735	25.382100944907204	41.791453561413014	18.82610015897305
22	11.342458902192876	23.660786053241083	39.76707769435497	25.229677350211073
23	12.133156299679076	24.387775151381636	36.944264169142556	26.53480437979673
24	13.850898763344507	23.03977898578769	37.11216828517502	25.99715396569278
25	13.056628937857614	25.324346692229373	33.98807999857103	27.630944371341982
26	11.922383047638327	25.698260822968333	37.045482962495456	25.33387316689788
27	14.14621947806827	24.45981911606223	39.08772096955696	22.306240436312542
28	11.583002387572714	21.74953707287158	38.80430834816883	27.863152191386874
29	13.519853768613837	20.988014504057684	36.7436127964371	28.748518930891382
30	19.23871559305282	22.12642822694444	35.20389632813942	23.43095985186332
31	17.98003012747614	27.6107006126714	29.170660839639662	25.2386084202128
32	14.908337451548945	28.39068072615553	28.64491851887135	28.05606330342417
33	18.200329854185394	23.841193667275963	28.787220234232198	29.17125624430644
34	18.93803623632802	20.84333117002971	28.622888546200425	31.595744047441844
35	16.38375021583419	20.90406244604145	29.41418134835341	33.29800598977094
36	15.833596303727829	22.30028638964472	32.66151840098123	29.204598905646222
37	16.35278917316154	23.038588176454127	32.97708287437557	27.63153977600876
38	15.471590266324508	28.207891493453523	28.45617523950153	27.86434300072044
39	19.21906723904902	29.342732788339593	26.32164950908885	25.116550463522536
40	23.918596273957597	23.787607247265605	30.448399254553358	21.845397224223444
41	20.524194268634677	19.70134501914226	33.71717087518532	26.057289837037743
42	17.38522086536114	18.165796383512053	35.040755449441214	29.408227301685592
43	16.105696236447102	19.295279036397087	35.350365876167736	29.248658850988075
44	17.10954850464118	21.62390668818062	31.26648526671152	30.00005954046668
45	22.901049698427535	19.61798836579281	29.01287860294249	28.468083332837164
46	24.072210677987293	20.135990425892956	33.895792275219854	21.896006620899893
47	17.153608449983032	22.852226515751433	37.34735312855382	22.646811905711715
48	15.75738450637976	21.802528088215155	38.00229826201378	24.437789143391306
49	21.16782671342578	17.047626419295874	37.4557167779081	24.32883008937024
50	21.391103463468948	14.832125654200878	33.9987972825731	29.777973599757075
51	18.1610331461778	14.502271468803773	31.703512292129343	35.633183092889084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	733.0
2	1101.0
3	4404.0
4	7707.0
5	4936.0
6	2165.0
7	2070.0
8	1975.0
9	1963.0
10	1951.0
11	1771.5
12	1592.0
13	1494.5
14	1397.0
15	1271.5
16	1146.0
17	1069.5
18	993.0
19	951.5
20	910.0
21	877.0
22	844.0
23	787.0
24	730.0
25	782.0
26	839.0
27	844.0
28	873.0
29	902.0
30	972.0
31	1042.0
32	1172.5
33	1303.0
34	1501.0
35	1699.0
36	1862.0
37	2025.0
38	2321.5
39	2618.0
40	3531.0
41	4444.0
42	5690.5
43	6937.0
44	8484.5
45	10032.0
46	13274.0
47	16516.0
48	20821.5
49	25127.0
50	25287.5
51	25448.0
52	21311.0
53	17174.0
54	12721.0
55	8268.0
56	6534.5
57	4801.0
58	4206.0
59	3611.0
60	3382.5
61	3154.0
62	2817.5
63	2481.0
64	2215.0
65	1949.0
66	1573.5
67	1198.0
68	1037.0
69	876.0
70	753.0
71	630.0
72	541.5
73	453.0
74	381.0
75	245.0
76	181.0
77	148.0
78	115.0
79	85.5
80	56.0
81	43.0
82	30.0
83	20.0
84	10.0
85	8.0
86	6.0
87	3.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167953.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.19310164153066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25741168492657	43.41333587372658
2	12.01869079658395	13.50735027061142
3	4.723558456419928	7.962942013539502
4	2.130793193328954	4.789435139592624
5	1.1496323295683315	3.230070317291147
6	0.6336222424717625	2.1363119444130203
7	0.4238275869376338	1.667133066988979
8	0.2935006039543114	1.3194167415884206
9	0.18648413825255886	0.9431209921823367
>10	1.0733433639195575	10.6881091733997
>50	0.07416982771408591	2.853774567885063
>100	0.02860836211829028	2.6019183938363706
>500	0.0052978448367204225	2.139884372413711
>1k	0.0010595689673440843	2.7471971325311246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4614	2.7471971325311246	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	814	0.48465939876036745	Illumina PCR Primer Index 4 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	798	0.47513292409185903	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	795	0.47334671009151374	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	603	0.3590290140694123	TruSeq Adapter, Index 13 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	584	0.34771632540055847	TruSeq Adapter, Index 13 (96% over 25bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	412	0.24530672271409265	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	266	0.158377641363953	Illumina PCR Primer Index 4 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.14468333402797212	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGT	232	0.13813388269337254	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	203	0.12086714735670097	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	189	0.11253148202175609	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	183	0.10895905402106541	TruSeq Adapter, Index 13 (95% over 24bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.10776824468750187	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTG	180	0.10717284002072008	Illumina PCR Primer Index 4 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011908093335635565	0.0	0.0	0.5453906747721089	0.0
2	0.0011908093335635565	0.0	0.0	1.901127101034218	0.0
3	0.0011908093335635565	0.0	0.0	2.7126636618577815	0.0
4	0.0011908093335635565	0.0	0.0	3.9826618161033145	0.0
5	0.0011908093335635565	0.0	0.0	6.682821979958679	0.0
6	0.0011908093335635565	0.0	0.0	8.945955118396219	0.0
7	0.0011908093335635565	0.0	0.0	10.97211719945461	0.0
8	0.0011908093335635565	0.0	0.0	13.927110560692574	0.0
9	0.0011908093335635565	0.0	0.0	15.326906932296536	0.0
10	0.0011908093335635565	0.0	0.0	18.49207814090847	0.0
11	0.0011908093335635565	0.0	0.0	25.86854655766792	0.0
12	0.0011908093335635565	0.0	0.0	32.727608318994	0.0
13	0.0011908093335635565	0.0	0.0	34.98716902943085	0.0
14	0.0011908093335635565	0.0	0.0	35.7665537382482	0.0
15	0.0011908093335635565	0.0	0.0	36.89127315379898	0.0
16	0.0011908093335635565	0.0	0.0	39.59917357832251	0.0
17	0.0011908093335635565	0.0	0.0	42.7429102189303	0.0
18	0.0011908093335635565	0.0	0.0	45.59668478681536	0.0
19	0.0011908093335635565	0.0	0.0	47.26798568647181	0.0
20	0.0011908093335635565	0.0	0.0	48.68683500741279	0.0
21	0.0011908093335635565	0.0	0.0	50.33551052973153	0.0
22	0.0011908093335635565	0.0	0.0	51.71268152399779	0.0
23	0.0011908093335635565	0.0	0.0	52.76476157020119	0.0
24	0.0011908093335635565	0.0	0.0	53.39231808898918	0.0
25	0.0011908093335635565	0.0	0.0	53.759087363726756	0.0
26	0.0011908093335635565	0.0	0.0	54.04071377111454	0.0
27	0.0011908093335635565	0.0	0.0	54.28780670782898	0.0
28	0.0011908093335635565	0.0	0.0	54.501556983203635	0.0
29	0.0011908093335635565	0.0	0.0	54.69089566724024	0.0
30	0.0011908093335635565	0.0	0.0	54.92488970128548	0.0
31	0.0011908093335635565	0.0	0.0	55.11006055265461	0.0
32	0.0011908093335635565	0.0	0.0	55.31130733002685	0.0
33	0.0011908093335635565	0.0	0.0	55.47087578072437	0.0
34	0.0011908093335635565	0.0	0.0	55.635802873422925	0.0
35	0.0011908093335635565	0.0	0.0	55.80847022678964	0.0
36	0.0011908093335635565	0.0	0.0	55.967443272820375	0.0
37	0.0011908093335635565	0.0	0.0	56.12046227218329	0.0
38	0.0011908093335635565	0.0	0.0	56.292534220883226	0.0
39	0.0011908093335635565	0.0	0.0	56.47651426291879	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTT	20	7.0160744E-4	45.000004	9
TACCATT	20	7.0160744E-4	45.000004	4
GATAACG	20	7.0160744E-4	45.000004	17
TTGCTAG	20	7.0160744E-4	45.000004	1
TTTCGCG	20	7.0160744E-4	45.000004	1
TCGGGAC	20	7.0160744E-4	45.000004	5
TAACGAA	20	7.0160744E-4	45.000004	19
GAAAGGT	20	7.0160744E-4	45.000004	9
CTAGTTA	20	7.0160744E-4	45.000004	45
AACGAGA	20	7.0160744E-4	45.000004	24
CTACCAT	20	7.0160744E-4	45.000004	3
GTTTTCG	20	7.0160744E-4	45.000004	1
TGCTAAC	20	7.0160744E-4	45.000004	39
ATAACGA	20	7.0160744E-4	45.000004	18
GCTACCA	20	7.0160744E-4	45.000004	2
AGCTACC	20	7.0160744E-4	45.000004	1
CGATAAC	20	7.0160744E-4	45.000004	16
GCGTAGG	20	7.0160744E-4	45.000004	2
TAACTAG	20	7.0160744E-4	45.000004	42
TTGATCC	25	3.876546E-5	45.0	17
>>END_MODULE