##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933664.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312075
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20827044780902	33.0	31.0	34.0	30.0	34.0
2	32.38274773692221	34.0	31.0	34.0	30.0	34.0
3	32.50984859408796	34.0	31.0	34.0	31.0	34.0
4	36.029855002803814	37.0	35.0	37.0	35.0	37.0
5	36.05406713129857	37.0	35.0	37.0	35.0	37.0
6	36.0084595049267	37.0	35.0	37.0	35.0	37.0
7	36.11702635584395	37.0	35.0	37.0	35.0	37.0
8	35.99839461667868	37.0	35.0	37.0	35.0	37.0
9	37.82454217736121	39.0	38.0	39.0	35.0	39.0
10	37.006472803012095	39.0	37.0	39.0	32.0	39.0
11	36.99315228711047	39.0	37.0	39.0	33.0	39.0
12	37.12663622526636	39.0	37.0	39.0	34.0	39.0
13	37.16422013938957	39.0	37.0	39.0	33.0	39.0
14	38.35026195626052	40.0	38.0	41.0	34.0	41.0
15	38.37819434430826	40.0	38.0	41.0	34.0	41.0
16	38.44993030521509	40.0	38.0	41.0	34.0	41.0
17	38.42993511175198	40.0	38.0	41.0	34.0	41.0
18	38.19134502923976	39.0	38.0	41.0	34.0	41.0
19	37.9242361611792	39.0	37.0	41.0	34.0	41.0
20	37.54256188416246	39.0	35.0	41.0	34.0	41.0
21	37.44490266762797	39.0	35.0	41.0	33.0	41.0
22	37.46248818393015	39.0	35.0	40.0	33.0	41.0
23	37.401105503484736	39.0	35.0	40.0	33.0	41.0
24	37.30745173435873	39.0	35.0	41.0	33.0	41.0
25	37.22197548666186	39.0	35.0	40.0	33.0	41.0
26	37.18609308659777	39.0	35.0	40.0	33.0	41.0
27	37.10374108787951	39.0	35.0	40.0	33.0	41.0
28	36.962672434510935	39.0	35.0	40.0	33.0	41.0
29	36.8587262677241	39.0	35.0	40.0	32.0	41.0
30	36.67804854602259	39.0	35.0	40.0	31.0	41.0
31	36.31013698630137	39.0	35.0	40.0	31.0	41.0
32	35.84943362973644	39.0	35.0	40.0	29.0	41.0
33	35.38987102459345	39.0	35.0	40.0	24.0	41.0
34	34.97833213169911	39.0	35.0	41.0	21.0	41.0
35	34.68338700632861	39.0	35.0	41.0	18.0	41.0
36	34.42719218136666	38.0	34.0	40.0	16.0	41.0
37	34.18746134743251	38.0	34.0	40.0	15.0	41.0
38	34.03521268925739	38.0	33.0	40.0	15.0	41.0
39	34.02313866858928	38.0	33.0	40.0	15.0	41.0
40	33.91342145317632	38.0	33.0	40.0	15.0	41.0
41	33.71293118641352	38.0	33.0	40.0	13.0	41.0
42	33.52922214211328	38.0	33.0	40.0	12.0	41.0
43	33.38751902587519	38.0	33.0	40.0	12.0	41.0
44	33.31166866939037	38.0	32.0	40.0	11.0	41.0
45	33.34569895057278	38.0	33.0	40.0	10.0	41.0
46	33.10577265080509	38.0	32.0	40.0	10.0	41.0
47	32.87202755747817	37.0	31.0	40.0	10.0	41.0
48	32.73383321316991	37.0	31.0	40.0	10.0	41.0
49	32.73058078987423	37.0	31.0	40.0	10.0	41.0
50	32.64849795722182	37.0	31.0	40.0	10.0	41.0
51	31.497899543378995	35.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	0.0
11	1.0
12	6.0
13	3.0
14	8.0
15	13.0
16	19.0
17	41.0
18	103.0
19	182.0
20	394.0
21	638.0
22	1031.0
23	1825.0
24	3549.0
25	6657.0
26	9162.0
27	8939.0
28	7594.0
29	6560.0
30	6772.0
31	7192.0
32	8548.0
33	10985.0
34	16019.0
35	20930.0
36	27365.0
37	39416.0
38	65104.0
39	63001.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.242489786109108	4.885364095169431	42.894176079468075	28.977970039253385
2	34.48241608587679	4.468156693102619	45.54514139229352	15.504285828727069
3	18.55803893294881	4.294800929263799	59.262677241047825	17.884482896739566
4	14.893214772090044	4.547304333894096	60.32203797164143	20.23744292237443
5	13.485219899062725	5.473043338940959	61.26732356004165	19.77441320195466
6	17.317952415284786	6.932948810382119	63.652327164944325	12.09677160938877
7	72.2656412721301	1.847312344788913	22.68236802050789	3.2046783625731
8	73.30769846991909	1.9341504446046622	21.11832091644637	3.6398301690298807
9	67.90130577585516	4.91163983016903	22.98421853721061	4.2028358567652
10	28.562685251942643	22.901225666906992	32.94688776736361	15.589201313786749
11	20.375550749018664	23.303052150925257	36.85332051590163	19.46807658415445
12	16.70399743651366	20.4742449731635	43.45141392293519	19.370343667387647
13	20.335816710726586	21.055195065288792	44.347031963470315	14.2619562605143
14	19.04285828727069	21.8723063366178	43.303372586717934	15.781462789393574
15	16.14900264359529	22.25106144356325	44.054794520547944	17.54514139229352
16	16.223664183289273	20.86934230553553	42.59745253544821	20.30954097572699
17	16.144836978290474	21.581671072658818	41.133701834494914	21.139790114555794
18	17.427861892173354	19.969238163902908	43.43571256909397	19.16718737482977
19	17.667868300889207	22.85412160538332	42.774973964591844	16.703036129135622
20	17.997917167347595	24.141632620363694	41.131458783946165	16.728991428342546
21	17.240086517664025	24.884402787791394	41.47624769686774	16.399262997676843
22	16.192581911399504	23.07714491708724	40.093567251461984	20.63670592005127
23	14.854442041176	24.414643915725385	38.82944804934711	21.9014659937515
24	15.117519826964672	24.121765601217657	39.98718256829288	20.773532003524796
25	14.857325963310103	26.14531763197949	38.09821357045582	20.899142834254587
26	15.22742930385324	26.456140350877195	39.236401506048225	19.08002883922134
27	16.031082271889773	25.491308179123607	40.38356164383561	18.094047905151005
28	14.911159176480012	24.099975967315547	40.59889449651526	20.389970359689176
29	17.107426099495314	22.695185452215014	39.260434190499076	20.936954257790596
30	20.483858046943844	24.67740126572138	36.67099254986782	18.167748137466955
31	19.299206921413123	28.34030281182408	33.7322758952175	18.6282143715453
32	18.101417928382602	27.85932868701434	33.67684050308419	20.362412881518864
33	20.73187535047665	26.024513338139872	31.917968437074425	21.32564287430906
34	20.698870463830808	25.855002803813186	31.455900024032683	21.99022670832332
35	23.72025955299207	23.84170471841705	31.623167507810624	20.814868220780262
36	26.099174877833853	27.25562765360891	29.33685812705279	17.308339341504446
37	23.05535528318513	29.728430665705357	30.525995353681008	16.690218697428502
38	19.981414724024674	29.326604181687095	32.7398862452936	17.952094848994633
39	22.221260914844187	27.594648722262278	31.403028118240805	18.781062244652727
40	25.203556837298724	25.783225186253304	30.505167027156933	18.508050949291036
41	25.575582792597935	24.332932788592483	31.05151005367299	19.039974365136587
42	26.93487142513819	24.324601457982855	31.480573580068892	17.25995353681006
43	24.275895217495794	24.75783064968357	33.00456621004566	17.961707922774973
44	21.65312825442602	25.489385564367538	31.696226868541217	21.161259312665226
45	23.842345590002402	24.47104061523672	30.194664744051913	21.491949050708964
46	24.81038211968277	25.166386285348075	32.06729151646239	17.955940078506767
47	21.92165344869022	26.697748938556437	34.00817111271329	17.372426500040056
48	21.11095089321477	25.72041977088841	35.28158295281583	17.88704638308099
49	23.531522871104702	23.06528879275815	34.27829848594088	19.124889850196265
50	22.240166626612194	21.664663942962427	32.56588960986942	23.529279820555956
51	20.981494832972842	21.480092926379875	30.968517183369382	26.569895057277897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	612.0
2	914.0
3	6616.5
4	12319.0
5	8187.5
6	4056.0
7	4002.5
8	3949.0
9	4025.0
10	4101.0
11	4101.5
12	4102.0
13	3957.0
14	3812.0
15	3717.0
16	3622.0
17	3499.5
18	3377.0
19	3232.5
20	3088.0
21	2954.5
22	2821.0
23	2772.5
24	2724.0
25	2745.0
26	2991.0
27	3216.0
28	3540.5
29	3865.0
30	4201.0
31	4537.0
32	4959.0
33	5381.0
34	6466.5
35	7552.0
36	8184.0
37	8816.0
38	9862.5
39	10909.0
40	12523.5
41	14138.0
42	16165.5
43	18193.0
44	20347.5
45	22502.0
46	25941.0
47	29380.0
48	32660.5
49	35941.0
50	34382.0
51	32823.0
52	27724.5
53	22626.0
54	18360.5
55	14095.0
56	11337.0
57	8579.0
58	7257.0
59	5935.0
60	5213.5
61	4492.0
62	3611.5
63	2731.0
64	2223.5
65	1716.0
66	1402.0
67	1088.0
68	824.5
69	561.0
70	465.5
71	370.0
72	294.0
73	218.0
74	192.5
75	170.5
76	174.0
77	120.0
78	66.0
79	46.0
80	26.0
81	20.0
82	14.0
83	8.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	312075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.01497141352272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22635907649413	39.128969169072384
2	10.565417302463553	10.991197579191603
3	4.82797588875919	7.5338108351695885
4	2.792537580397026	5.810150496621569
5	1.7429061992921773	4.5328608066317075
6	1.1389710403578046	3.554612766302487
7	0.8103090810947481	2.9503742582482837
8	0.624019251181102	2.5966674809338306
9	0.446136885006034	2.088521758809624
>10	1.7569499035330745	14.270506778597799
>50	0.0486937434438524	1.7893135823297286
>100	0.016642165480810312	1.4440187018120583
>500	0.0018491294978678124	0.586712749626125
>1k	6.163764992892708E-4	0.422882031014677
>5k	6.163764992892708E-4	2.299401005638562
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7172	2.298165505086918	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	1319	0.4226548105423376	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	651	0.20860370103340542	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	635	0.20347672835055675	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	544	0.1743170712168549	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	445	0.14259392774172874	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	429	0.13746695505888007	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.408715853560842E-4	0.0	0.0	0.2275094128014099	0.0
2	6.408715853560842E-4	0.0	0.0	0.7575102138908916	0.0
3	6.408715853560842E-4	0.0	0.0	1.0757029560201874	0.0
4	6.408715853560842E-4	0.0	0.0	1.5941680685732595	0.0
5	6.408715853560842E-4	0.0	0.0	2.8009292637987664	0.0
6	6.408715853560842E-4	0.0	0.0	3.9580229111591763	0.0
7	6.408715853560842E-4	0.0	0.0	4.939838179924697	0.0
8	6.408715853560842E-4	0.0	0.0	6.488824801730353	0.0
9	6.408715853560842E-4	0.0	0.0	7.257229832572298	0.0
10	6.408715853560842E-4	0.0	0.0	9.068973804373949	0.0
11	6.408715853560842E-4	0.0	0.0	13.223103420652087	0.0
12	6.408715853560842E-4	0.0	0.0	16.733477529440037	0.0
13	6.408715853560842E-4	0.0	0.0	17.945686133141074	0.0
14	6.408715853560842E-4	0.0	0.0	18.367379636305376	0.0
15	6.408715853560842E-4	0.0	0.0	18.907954818553232	0.0
16	6.408715853560842E-4	0.0	0.0	20.44957141712729	0.0
17	6.408715853560842E-4	0.0	0.0	22.651926620203476	0.0
18	6.408715853560842E-4	0.0	0.0	24.703356564928303	0.0
19	6.408715853560842E-4	0.0	0.0	25.910758631739164	0.0
20	6.408715853560842E-4	0.0	0.0	26.979732436113114	0.0
21	6.408715853560842E-4	0.0	0.0	28.34030281182408	0.0
22	6.408715853560842E-4	0.0	0.0	29.507009532964833	0.0
23	6.408715853560842E-4	0.0	0.0	30.422815028438677	0.0
24	6.408715853560842E-4	0.0	0.0	30.979412000320437	0.0
25	6.408715853560842E-4	0.0	0.0	31.3652166947048	0.0
26	6.408715853560842E-4	0.0	0.0	31.661940238724664	0.0
27	6.408715853560842E-4	0.0	0.0	31.951293759512936	0.0
28	6.408715853560842E-4	0.0	0.0	32.22558679804534	0.0
29	6.408715853560842E-4	0.0	0.0	32.49315068493151	0.0
30	6.408715853560842E-4	0.0	0.0	32.769686774012655	0.0
31	6.408715853560842E-4	0.0	0.0	33.0215493070576	0.0
32	6.408715853560842E-4	0.0	0.0	33.28238404229752	0.0
33	6.408715853560842E-4	0.0	0.0	33.508611711928225	0.0
34	6.408715853560842E-4	0.0	0.0	33.73772330369302	0.0
35	9.613073780341264E-4	0.0	0.0	33.96202835856765	0.0
36	9.613073780341264E-4	0.0	0.0	34.186012977649604	0.0
37	9.613073780341264E-4	0.0	0.0	34.42377633581671	0.0
38	9.613073780341264E-4	0.0	0.0	34.66378274453256	0.0
39	9.613073780341264E-4	0.0	0.0	34.91244091965073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.884254E-5	45.0	21
GTGCATA	20	7.0253795E-4	45.0	11
CGGGTTA	20	7.0253795E-4	45.0	6
CGGGTAC	25	3.884254E-5	45.0	6
TCTTGCG	45	3.8198777E-10	45.0	1
CACGGGA	35	1.2081182E-7	45.0	4
GGATGAT	35	1.2081182E-7	45.0	8
GACACGG	25	3.884254E-5	45.0	16
TTTCGCG	40	6.7884685E-9	45.0	1
TACGGGT	20	7.0253795E-4	45.0	4
TGTTCCG	20	7.0253795E-4	45.0	1
AATAGGC	20	7.0253795E-4	45.0	44
ATCGGGG	20	7.0253795E-4	45.0	4
CCGCACT	20	7.0253795E-4	45.0	34
CGTTTTT	4540	0.0	44.306168	1
CGTTTGG	120	0.0	41.249996	2
TTGGGCG	425	0.0	39.705883	5
GTTTGCG	40	3.4474397E-7	39.375	1
ATTTGCG	40	3.4474397E-7	39.375	1
CTTGGGC	435	0.0	39.310345	4
>>END_MODULE