##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314233
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.125222366842436	33.0	31.0	34.0	30.0	34.0
2	32.312583337841666	34.0	31.0	34.0	30.0	34.0
3	32.456963463417274	34.0	31.0	34.0	31.0	34.0
4	35.932788726836456	37.0	35.0	37.0	35.0	37.0
5	36.018931811744785	37.0	35.0	37.0	35.0	37.0
6	36.00600828047977	37.0	35.0	37.0	35.0	37.0
7	36.04662145605331	37.0	35.0	37.0	35.0	37.0
8	35.96007421244745	37.0	35.0	37.0	35.0	37.0
9	37.787256589855296	39.0	38.0	39.0	35.0	39.0
10	37.03739263540112	39.0	37.0	39.0	33.0	39.0
11	37.01305082534298	39.0	37.0	39.0	33.0	39.0
12	37.11418915263515	39.0	37.0	39.0	34.0	39.0
13	37.168381423975205	39.0	37.0	39.0	34.0	39.0
14	38.37918041707905	40.0	38.0	41.0	34.0	41.0
15	38.39998981647376	40.0	38.0	41.0	34.0	41.0
16	38.52493850104859	40.0	38.0	41.0	34.0	41.0
17	38.54140717238482	40.0	38.0	41.0	34.0	41.0
18	38.35035467312472	39.0	38.0	41.0	34.0	41.0
19	38.115653034531704	39.0	37.0	41.0	34.0	41.0
20	37.69140414915047	39.0	35.0	41.0	34.0	41.0
21	37.629058692117	39.0	35.0	41.0	34.0	41.0
22	37.62189203552778	39.0	35.0	41.0	34.0	41.0
23	37.49146970560062	39.0	35.0	41.0	33.0	41.0
24	37.41182498337221	39.0	35.0	41.0	33.0	41.0
25	37.34066441143992	39.0	35.0	41.0	33.0	41.0
26	37.33545490129936	39.0	35.0	41.0	33.0	41.0
27	37.271225491912055	39.0	35.0	41.0	33.0	41.0
28	37.19609334474737	39.0	35.0	41.0	33.0	41.0
29	37.01694602412859	39.0	35.0	41.0	33.0	41.0
30	36.73559428831472	39.0	35.0	41.0	32.0	41.0
31	36.37527567123758	39.0	35.0	41.0	31.0	41.0
32	36.0052636101237	39.0	35.0	41.0	29.0	41.0
33	35.475195157733275	39.0	35.0	41.0	24.0	41.0
34	35.06518411497201	39.0	35.0	41.0	21.0	41.0
35	34.83323839316685	39.0	35.0	41.0	18.0	41.0
36	34.57119716897971	39.0	35.0	41.0	18.0	41.0
37	34.360582752288906	38.0	34.0	41.0	15.0	41.0
38	34.21303936887596	38.0	34.0	40.0	15.0	41.0
39	34.244169135641386	38.0	34.0	40.0	15.0	41.0
40	34.096485728742685	38.0	34.0	40.0	15.0	41.0
41	33.922414259482615	38.0	33.0	40.0	14.0	41.0
42	33.66361266957958	38.0	33.0	40.0	13.0	41.0
43	33.556354679489424	38.0	33.0	40.0	12.0	41.0
44	33.4741322521823	38.0	33.0	40.0	12.0	41.0
45	33.49107827631089	38.0	33.0	40.0	12.0	41.0
46	33.219534549203935	38.0	32.0	40.0	10.0	41.0
47	33.09091661283188	38.0	32.0	40.0	10.0	41.0
48	32.93763544885483	37.0	32.0	40.0	10.0	41.0
49	32.93146168607371	37.0	32.0	40.0	10.0	41.0
50	32.763544885483064	37.0	31.0	40.0	10.0	41.0
51	31.737128181954155	36.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	4.0
11	3.0
12	11.0
13	7.0
14	4.0
15	13.0
16	23.0
17	52.0
18	97.0
19	184.0
20	367.0
21	626.0
22	1030.0
23	1894.0
24	3576.0
25	6381.0
26	9102.0
27	8821.0
28	7596.0
29	6619.0
30	6694.0
31	7109.0
32	8434.0
33	10592.0
34	15447.0
35	20075.0
36	25755.0
37	37809.0
38	65543.0
39	70331.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.195526886100442	5.045937250384269	42.85355134565752	28.90498451785777
2	34.54984040504976	4.411376271747398	45.55123109285149	15.487552230351366
3	18.729414160829702	4.359822170173088	58.86682811798888	18.043935551008328
4	14.992060031887167	4.6586450181871415	60.04366186874071	20.305633081184983
5	13.437481104785304	5.52965474663705	61.12152447387766	19.911339674699985
6	17.34509106300102	6.924797841092438	63.606941346071224	12.123169749835313
7	72.21997689612485	1.8823611778521032	22.587697663835435	3.3099642621876124
8	73.24023893098433	1.9768770307383374	21.037255794267313	3.745628244010018
9	67.80096297969978	4.9848360929628655	22.981036364735722	4.233164562601637
10	28.636394013359514	22.871245222494135	32.744492144364216	15.747868619782135
11	20.321544840930137	23.305954498731833	36.94933377461947	19.423166885718558
12	16.85819121479921	20.531580069566214	43.345861192172684	19.26436752346189
13	20.23466663272158	21.159139873915215	44.23310091556266	14.37309257780055
14	19.117979333806442	21.859257302702133	43.10845773677494	15.914305626716482
15	16.20771847641718	22.302240694007313	43.9746939373013	17.515346892274202
16	16.225539647331757	21.027390503225313	42.47835205086671	20.268717798576215
17	16.334694319183534	21.700139705250564	40.90276960090124	21.06239637466466
18	17.505481601232205	20.06600197942291	43.450560571295824	18.977955848049056
19	17.805895625220774	22.969898132914114	42.60946495116681	16.614741290698305
20	17.969786750595894	24.10313366196421	41.06252366874262	16.86455591869727
21	17.25853108998737	24.884401065451435	41.32825005648675	16.528817788074456
22	16.294278449430834	23.1309251415351	39.88409874201627	20.690697667017783
23	15.061435304376053	24.428370031155225	38.68403382203651	21.82616084243221
24	15.250148774953617	24.120318362488984	39.92865166930272	20.700881193254688
25	14.981240035260459	26.16243360818246	38.10325459133828	20.753071765218802
26	15.364076974728944	26.450118224374908	39.184299548424256	19.001505252471894
27	16.164438489910353	25.399305610804724	40.36272447515061	18.07353142413432
28	15.19350291026086	24.203695983553605	40.340448011507384	20.262353094678154
29	17.33077047923038	22.79073171818364	39.1575677920524	20.720930010533582
30	20.730158831185776	24.7599711042443	36.46816215992592	18.041707904644007
31	19.2551387028097	28.42126702160499	33.60881893372116	18.714775341864158
32	18.284203123160204	27.6234513879828	33.59577129073013	20.49657419812687
33	20.837085856673234	25.988358956570444	31.88812123487985	21.286433951876475
34	20.742570003786998	25.933622503047104	31.592162503619925	21.731644989545973
35	23.81767669213609	24.051579560389904	31.42477079110087	20.705972956373138
36	25.878886049523757	27.37109087842461	29.460941403353562	17.289081668698067
37	22.840694643783436	29.864781865685654	30.57571929109928	16.718804199431634
38	20.098461969303035	29.262999112123804	32.85873857933444	17.77980033923872
39	22.406621837935546	27.57667081433204	31.321662587952254	18.695044759780163
40	25.244961541276695	25.870611934456278	30.566172235252186	18.318254289014842
41	25.588655551772092	24.352630054768277	31.025703856692328	19.033010536767303
42	26.74193989810109	24.63108585030853	31.383718450958366	17.243255800632014
43	24.053488971559318	24.773336982430237	33.16265319046695	18.0105208555435
44	21.690274414208567	25.769731377671985	31.53297075736794	21.007023450751515
45	24.033440154280424	24.60912762186021	30.195428233190018	21.162003990669344
46	24.728784055143795	25.289514468563134	32.05965000493264	17.922051471360422
47	21.756149099553515	27.024532751175084	34.02156998151054	17.197748167760864
48	21.30330041720634	25.561923795400226	35.195857850703135	17.93891793669029
49	23.693564966123866	23.016678706564875	34.107175248939484	19.182581078371783
50	22.172082499291925	21.685500886285016	32.47431046389144	23.668106150531614
51	21.08149048635885	21.617398554575747	31.077257958266635	26.22385300079877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	329.0
1	618.5
2	908.0
3	6562.0
4	12216.0
5	8281.0
6	4346.0
7	4197.0
8	4048.0
9	4118.5
10	4189.0
11	4083.5
12	3978.0
13	3905.0
14	3832.0
15	3740.5
16	3649.0
17	3502.0
18	3355.0
19	3168.5
20	2982.0
21	2906.5
22	2831.0
23	2740.5
24	2650.0
25	2737.0
26	3055.5
27	3287.0
28	3624.5
29	3962.0
30	4308.5
31	4655.0
32	5065.0
33	5475.0
34	6594.0
35	7713.0
36	8205.5
37	8698.0
38	9893.5
39	11089.0
40	12752.5
41	14416.0
42	16316.5
43	18217.0
44	20272.0
45	22327.0
46	25885.5
47	29444.0
48	32680.5
49	35917.0
50	34540.0
51	33163.0
52	27899.0
53	22635.0
54	18418.0
55	14201.0
56	11496.0
57	8791.0
58	7445.0
59	6099.0
60	5397.0
61	4695.0
62	3754.0
63	2813.0
64	2296.0
65	1779.0
66	1451.0
67	1123.0
68	833.0
69	543.0
70	466.0
71	389.0
72	300.5
73	212.0
74	201.5
75	174.5
76	158.0
77	116.0
78	74.0
79	44.5
80	15.0
81	11.5
82	8.0
83	5.5
84	3.0
85	3.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.23358406390302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.436074815167	39.402965553089025
2	10.434867621012794	10.901010701557428
3	4.8368294337358675	7.579348104894085
4	2.7764916333228995	5.801044365275803
5	1.7170281065649884	4.484326597217327
6	1.1902409364188062	3.7302330005238216
7	0.7975030069153335	2.915950824704925
8	0.6075654585287584	2.5388257161908547
9	0.41312682643087084	1.9421185335687319
>10	1.7257954607382484	14.281527823047504
>50	0.04237040362233432	1.5456824046386821
>100	0.019035978439019764	1.5787194014591395
>500	0.0018421914618406224	0.5981941657296415
>1k	6.140638206135409E-4	0.4342921717951391
>5k	6.140638206135409E-4	2.265760636307875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7064	2.2480134167958172	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	1354	0.4308904538988585	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	670	0.2132175805851072	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	642	0.2043069951278192	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	553	0.17598406278143924	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	486	0.15466230472292855	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	440	0.14002348575738385	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.182351949031451E-4	0.0	0.0	0.23072051630478022	0.0
2	3.182351949031451E-4	0.0	0.0	0.7602638806236137	0.0
3	3.182351949031451E-4	0.0	0.0	1.08772789617895	0.0
4	6.364703898062902E-4	0.0	0.0	1.6089971454303018	0.0
5	6.364703898062902E-4	0.0	0.0	2.7753291347503284	0.0
6	6.364703898062902E-4	0.0	0.0	3.8974264319788183	0.0
7	6.364703898062902E-4	0.0	0.0	4.87504495072128	0.0
8	6.364703898062902E-4	0.0	0.0	6.427396231458822	0.0
9	6.364703898062902E-4	0.0	0.0	7.1739759987016	0.0
10	6.364703898062902E-4	0.0	0.0	9.002873663809975	0.0
11	6.364703898062902E-4	0.0	0.0	13.132930023262993	0.0
12	6.364703898062902E-4	0.0	0.0	16.511314852354783	0.0
13	6.364703898062902E-4	0.0	0.0	17.661735081929653	0.0
14	6.364703898062902E-4	0.0	0.0	18.064939073871937	0.0
15	6.364703898062902E-4	0.0	0.0	18.596073614165284	0.0
16	6.364703898062902E-4	0.0	0.0	20.137923133471023	0.0
17	6.364703898062902E-4	0.0	0.0	22.32929068557408	0.0
18	6.364703898062902E-4	0.0	0.0	24.302667129168483	0.0
19	6.364703898062902E-4	0.0	0.0	25.485547348623474	0.0
20	6.364703898062902E-4	0.0	0.0	26.564682894540038	0.0
21	6.364703898062902E-4	0.0	0.0	27.902861889107765	0.0
22	6.364703898062902E-4	0.0	0.0	29.031960360624122	0.0
23	6.364703898062902E-4	0.0	0.0	29.896605385175967	0.0
24	6.364703898062902E-4	0.0	0.0	30.418192869622224	0.0
25	6.364703898062902E-4	0.0	0.0	30.81630509844606	0.0
26	6.364703898062902E-4	0.0	0.0	31.125947943086818	0.0
27	6.364703898062902E-4	0.0	0.0	31.421270203956936	0.0
28	6.364703898062902E-4	0.0	0.0	31.67554012468455	0.0
29	6.364703898062902E-4	0.0	0.0	31.94476709957261	0.0
30	9.547055847094353E-4	0.0	0.0	32.19458172757158	0.0
31	9.547055847094353E-4	0.0	0.0	32.45044282427371	0.0
32	9.547055847094353E-4	0.0	0.0	32.70916803772997	0.0
33	9.547055847094353E-4	0.0	0.0	32.933842085331584	0.0
34	9.547055847094353E-4	0.0	0.0	33.145468489942175	0.0
35	0.0012729407796125804	0.0	0.0	33.37364312468773	0.0
36	0.0012729407796125804	0.0	0.0	33.59768070189955	0.0
37	0.0012729407796125804	0.0	0.0	33.83540239249219	0.0
38	0.0012729407796125804	0.0	0.0	34.087762902050386	0.0
39	0.0012729407796125804	0.0	0.0	34.33566811887994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	25	3.884315E-5	45.0	6
GGCCGAT	80	0.0	45.0	8
TTCACGG	20	7.025454E-4	45.0	2
ATCCGGG	25	3.884315E-5	45.0	3
GCCGATG	20	7.025454E-4	45.0	9
GTACGGC	20	7.025454E-4	45.0	9
CGTTTTT	4550	0.0	44.010986	1
CTTTGCG	75	0.0	42.000004	1
TCGGGTA	50	1.0768417E-9	40.5	5
TCGGGAA	85	0.0	39.705883	5
CTTGTTG	165	0.0	39.545452	1
TTGGGTC	330	0.0	39.545452	5
CGTCGGG	40	3.4475306E-7	39.375	3
TTCTGCG	75	0.0	39.000004	1
TTCTCGG	110	0.0	38.863632	2
TGGGCAA	245	0.0	38.57143	6
CGGGTGT	70	0.0	38.571426	6
TTGTCCG	35	6.2338077E-6	38.571426	1
TTTCCGG	140	0.0	38.571426	2
CTTGGGC	550	0.0	38.454544	4
>>END_MODULE