##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933662.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1345665
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27884874764521	33.0	31.0	34.0	30.0	34.0
2	32.42468593594988	34.0	31.0	34.0	30.0	34.0
3	32.517391029713934	34.0	31.0	34.0	31.0	34.0
4	36.08612396101556	37.0	35.0	37.0	35.0	37.0
5	36.090078882931486	37.0	35.0	37.0	35.0	37.0
6	36.02356975918969	37.0	35.0	37.0	35.0	37.0
7	36.133425481081844	37.0	35.0	37.0	35.0	37.0
8	35.93067442491259	37.0	35.0	37.0	35.0	37.0
9	37.78640449145961	39.0	38.0	39.0	35.0	39.0
10	37.16585628666867	39.0	37.0	39.0	33.0	39.0
11	37.17784887026117	39.0	37.0	39.0	34.0	39.0
12	37.24392400783256	39.0	37.0	39.0	34.0	39.0
13	37.267105854726104	39.0	37.0	39.0	34.0	39.0
14	38.54773364841918	40.0	38.0	41.0	34.0	41.0
15	38.574151813415675	40.0	38.0	41.0	34.0	41.0
16	38.58753998952191	40.0	38.0	41.0	34.0	41.0
17	38.502577536013796	40.0	38.0	41.0	34.0	41.0
18	38.089676108095254	39.0	38.0	40.0	34.0	41.0
19	37.65652818494945	38.0	37.0	40.0	34.0	41.0
20	37.14792165955122	38.0	35.0	40.0	33.0	41.0
21	37.04875433335934	38.0	35.0	40.0	33.0	41.0
22	37.092251786291534	38.0	35.0	40.0	33.0	41.0
23	36.98559299677111	38.0	35.0	40.0	33.0	41.0
24	36.91607941055166	38.0	35.0	40.0	33.0	41.0
25	36.84631464740482	38.0	35.0	40.0	33.0	41.0
26	36.801489969643264	38.0	35.0	40.0	33.0	41.0
27	36.72678489817302	38.0	35.0	40.0	33.0	41.0
28	36.57187561540205	38.0	35.0	40.0	32.0	41.0
29	36.45213481810109	38.0	35.0	40.0	32.0	41.0
30	36.2301724426213	38.0	35.0	40.0	31.0	41.0
31	35.82841420412956	38.0	35.0	40.0	30.0	41.0
32	35.122489624089205	38.0	35.0	40.0	24.0	41.0
33	34.273564371518916	38.0	34.0	40.0	18.0	41.0
34	33.54246933672199	38.0	33.0	40.0	12.0	41.0
35	33.02826334934772	38.0	33.0	40.0	10.0	41.0
36	32.76267941872606	38.0	32.0	40.0	8.0	41.0
37	32.60875775174356	38.0	32.0	40.0	8.0	41.0
38	32.487284725395995	38.0	31.0	40.0	8.0	41.0
39	32.39318552537221	38.0	31.0	40.0	8.0	41.0
40	32.25594408712421	38.0	31.0	40.0	8.0	41.0
41	32.13040095417507	38.0	30.0	40.0	8.0	41.0
42	32.02784794135242	38.0	30.0	40.0	8.0	41.0
43	31.91571156268462	38.0	30.0	40.0	7.0	41.0
44	31.807095376635345	37.0	30.0	40.0	7.0	41.0
45	31.79749491886911	37.0	29.0	40.0	7.0	41.0
46	31.620337899848774	37.0	29.0	40.0	7.0	41.0
47	31.493231227682966	37.0	28.0	40.0	7.0	41.0
48	31.40665098668688	37.0	28.0	40.0	7.0	41.0
49	31.407120642953483	37.0	28.0	40.0	7.0	41.0
50	31.323694232962886	37.0	27.0	40.0	7.0	41.0
51	30.390360899629552	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	11.0
11	5.0
12	8.0
13	18.0
14	17.0
15	34.0
16	89.0
17	212.0
18	511.0
19	1006.0
20	1981.0
21	3314.0
22	5437.0
23	9649.0
24	18532.0
25	37983.0
26	59746.0
27	61144.0
28	47717.0
29	36946.0
30	32468.0
31	32843.0
32	35885.0
33	43972.0
34	65594.0
35	80557.0
36	100839.0
37	142284.0
38	252827.0
39	273964.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.346921410603677	3.601342087369442	37.12045717173294	34.93127933029395
2	39.01691728624881	7.445017890782624	39.23450487305533	14.30355994991324
3	16.49860849468478	7.306499017214536	60.75746935530017	15.437423132800513
4	13.181884049893547	4.042982465918338	64.62381053233904	18.151322951849085
5	14.646810313116562	4.837013669821241	61.620314119784645	18.895861897277555
6	15.493529221611618	7.683487346404938	63.8912359316769	12.931747500306539
7	63.250214577922435	1.589028472911163	31.556442353780472	3.6043145953859246
8	63.59108693471258	3.7224717890411063	28.703057596058457	3.9833836801878624
9	58.27000033440716	4.21910356589493	30.360899629551184	7.14999647014673
10	27.221188037141488	18.189891243362947	39.126528519356604	15.462392200138964
11	21.07471027335927	19.38966979151572	42.00406490471254	17.531555030412473
12	16.049611158795095	17.29100481917862	47.53322706617175	19.12615695585454
13	17.047630725329114	17.975647728074968	49.951510962981125	15.025210583614793
14	16.337944436393904	20.01798367349972	47.84727253811313	15.79679935199325
15	14.596129051435536	19.112929295181193	49.924461140031134	16.366480513352137
16	14.851467490051387	19.486722178253874	47.21479714490605	18.447013186788688
17	15.259369902613207	20.782884298841093	46.23632181858041	17.721423979965294
18	16.37420903419499	19.063808600208816	46.51395406731987	18.048028298276318
19	15.873638684219326	22.164357399501363	44.363418830095156	17.598585086184155
20	17.337821820438222	21.83700995418622	44.67099909710069	16.154169128274866
21	16.667967138923878	23.814173661349596	43.92601427546975	15.591844924256781
22	15.996328952599645	19.912162388112943	44.59891577770099	19.492592881586425
23	15.355158973444357	22.360022739686325	43.60817885580735	18.676639431061965
24	15.921867626786756	20.92095729620671	44.50342395767149	18.65375111933505
25	15.654936406906621	23.684572311830955	42.205080759327174	18.45541052193525
26	15.700787343060865	21.712536180996015	43.72083690963204	18.86583956631108
27	17.05699412558103	21.11543363318508	42.98313473264148	18.844437508592407
28	15.32394763927129	21.057989915766555	43.89666075880698	19.721401686155172
29	16.583250660454123	19.03423214544482	42.45343380410429	21.929083389996766
30	17.19878275796725	19.923234980474337	42.26363916725188	20.614343094306534
31	17.303340727447026	20.413921741295198	40.852886862629255	21.42985066862852
32	17.9678448945317	20.789200878376118	39.788134491125206	21.454819735966975
33	18.240944068546035	19.906365997480798	39.01676866084799	22.835921273125184
34	17.822489252525703	20.30780320510677	39.35466850962164	22.515039032745893
35	17.9167177566482	20.985683658265618	36.259693162859996	24.837905422226186
36	17.11458646840038	22.621008943533493	36.71300063537359	23.551403952692535
37	17.879784344543403	22.96039504631539	37.57391326964735	21.585907339493858
38	18.81820512534695	23.00676617137252	36.830191763923416	21.34483693935712
39	20.573619734480722	22.33393898184169	36.715601579888016	20.376839703789575
40	19.313127710091294	22.133369003429532	37.23720242408029	21.316300862398887
41	17.487264660966883	23.604091657284687	35.94497887661491	22.963664805133522
42	18.677010994564025	23.47597656177429	35.6356894174999	22.211323026161786
43	19.198314587954656	22.67191314331576	36.49831124388314	21.631461024846452
44	19.765171866697877	22.990566002682687	34.40997573690331	22.83428639371612
45	20.161853061497474	22.166884031315373	33.73031177893458	23.940951128252575
46	21.548379425785765	23.08382844169983	33.3244901219843	22.043302010530113
47	18.626626983684645	23.18043495223551	36.30019358458457	21.892744479495267
48	18.74344654873241	22.892027361936293	35.43281574537496	22.931710343956336
49	19.562000943771295	21.485510881237158	36.43900970895431	22.51347846603724
50	19.316694719711073	21.14278070693672	36.081937183474345	23.458587389877867
51	18.28315368238009	20.880159623680488	34.43286404863023	26.403822645309194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526.0
1	1370.5
2	2215.0
3	34956.0
4	67697.0
5	46397.5
6	25098.0
7	24768.5
8	24439.0
9	24632.5
10	24826.0
11	24645.5
12	24465.0
13	23954.5
14	23444.0
15	22758.5
16	22073.0
17	21097.0
18	20121.0
19	19490.5
20	18860.0
21	17928.5
22	16997.0
23	16564.0
24	16131.0
25	15888.5
26	15880.0
27	16114.0
28	16773.0
29	17432.0
30	19118.5
31	20805.0
32	22351.5
33	23898.0
34	27258.0
35	30618.0
36	32646.5
37	34675.0
38	37642.0
39	40609.0
40	44529.5
41	48450.0
42	52998.5
43	57547.0
44	66208.0
45	74869.0
46	104309.0
47	133749.0
48	127878.0
49	122007.0
50	118409.0
51	114811.0
52	100550.5
53	86290.0
54	73305.5
55	60321.0
56	52089.0
57	43857.0
58	39191.5
59	34526.0
60	30293.5
61	26061.0
62	22536.0
63	19011.0
64	16012.5
65	13014.0
66	10778.0
67	8542.0
68	7095.5
69	5649.0
70	4617.5
71	3586.0
72	3122.0
73	2658.0
74	2126.5
75	1391.5
76	1188.0
77	930.0
78	672.0
79	486.0
80	300.0
81	247.5
82	195.0
83	118.0
84	41.0
85	31.5
86	22.0
87	14.0
88	6.0
89	4.0
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1345665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.68117321558472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91397626893051	32.058647675646654
2	8.182947379269672	6.821496942587084
3	3.6519995257237508	4.566588744447747
4	2.1724915365816213	3.6220798418260154
5	1.5665732258346985	3.264830499045669
6	1.240274107845198	3.10176479543403
7	0.9523282079560156	2.7785909895731455
8	0.7721967471126876	2.5748853098332
9	0.655785521237455	2.460051891267379
>10	3.736832975980658	25.16072614091518
>50	0.09328949766837616	2.649580993691126
>100	0.0556022217086097	3.921735403216781
>500	0.0028513920754170063	0.8453904036490014
>1k	0.002138544056562755	1.4798974617256
>5k	3.564240094271258E-4	0.8788910689724088
>10k+	3.564240094271258E-4	3.8148418381690328
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38782	2.881995147380663	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	12575	0.9344822076817038	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	6130	0.45553685352595186	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	5702	0.42373101774958843	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	3312	0.2461236637647557	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC	2300	0.17091921094774704	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2102	0.15620529626615837	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	1916	0.14238313398951447	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTT	1896	0.1408968799812732	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	1421	0.10559834728554283	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTTCTT	1378	0.10240290116782409	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7156350206031964E-4	0.0	0.0	0.11503606023787495	0.0
2	3.7156350206031964E-4	0.0	0.0	0.45293590901152964	0.0
3	3.7156350206031964E-4	0.0	0.0	0.7024036442948282	0.0
4	3.7156350206031964E-4	0.0	0.0	1.3105044717667473	0.0
5	3.7156350206031964E-4	0.0	0.0	3.1483318656575	0.0
6	3.7156350206031964E-4	0.0	0.0	4.8003775085180935	0.0
7	3.7156350206031964E-4	0.0	0.0	6.175459716942924	0.0
8	4.4587620247238353E-4	0.0	0.0	8.009943039315134	0.0
9	4.4587620247238353E-4	0.0	0.0	9.101002106765057	0.0
10	5.201889028844474E-4	0.0	0.0	10.936451494242624	0.0
11	5.201889028844474E-4	0.0	0.0	12.87274321617936	0.0
12	5.201889028844474E-4	0.0	0.0	15.053003533568905	0.0
13	5.945016032965114E-4	0.0	0.0	15.82838224966838	0.0
14	5.945016032965114E-4	0.0	0.0	16.17802350510714	0.0
15	5.945016032965114E-4	0.0	0.0	16.588749800284617	0.0
16	6.688143037085753E-4	0.0	0.0	17.230960157245676	0.0
17	7.431270041206393E-4	0.0	0.0	18.02291060553704	0.0
18	7.431270041206393E-4	0.0	0.0	18.791378240498194	0.0
19	8.174397045327031E-4	0.0	0.0	19.405572709403902	0.0
20	8.174397045327031E-4	0.0	0.0	19.876789542716796	0.0
21	8.174397045327031E-4	0.0	0.0	20.438593557832	0.0
22	8.174397045327031E-4	0.0	0.0	20.96249809573705	0.0
23	8.174397045327031E-4	0.0	0.0	21.40458435048842	0.0
24	8.917524049447671E-4	0.0	0.0	21.76240000297251	0.0
25	8.917524049447671E-4	0.0	0.0	22.05013877896802	0.0
26	8.917524049447671E-4	0.0	0.0	22.310604793912304	0.0
27	8.917524049447671E-4	0.0	0.0	22.574266254974308	0.0
28	8.917524049447671E-4	0.0	0.0	22.834137768315294	0.0
29	8.917524049447671E-4	0.0	0.0	23.111324140852293	0.0
30	9.66065105356831E-4	0.0	0.0	23.41191901401909	0.0
31	9.66065105356831E-4	0.0	0.0	23.692523770775043	0.0
32	9.66065105356831E-4	0.0	0.0	24.02581623212315	0.0
33	9.66065105356831E-4	0.0	0.0	24.29579427272018	0.0
34	0.0010403778057688948	0.0	0.0	24.57097420234605	0.0
35	0.001114690506180959	0.0	0.0	24.84704588437687	0.0
36	0.001114690506180959	0.0	0.0	25.130177272946835	0.0
37	0.001114690506180959	0.0	0.0	25.42103718235965	0.0
38	0.0011890032065930227	0.0	0.0	25.72668531915447	0.0
39	0.0012633159070050868	0.0	0.0	26.04675011982923	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCCG	45	3.8562575E-10	45.0	6
CGTTTTT	30255	0.0	44.22657	1
CCGATGA	380	0.0	40.263153	18
TTATGCG	45	1.9286745E-8	40.0	1
GTTTTTT	34180	0.0	39.80617	2
CGTTTGG	770	0.0	39.15584	2
GCTTGCG	150	0.0	39.0	1
GCGAGAC	250	0.0	38.7	21
AGACACG	250	0.0	38.7	24
TCTCGCG	70	0.0	38.571426	1
GCGATAG	35	6.2490853E-6	38.571426	9
CGATGAA	410	0.0	38.414635	19
TCAAGCG	250	0.0	37.800003	17
GCTACGA	155	0.0	37.741936	10
CGGGTCA	90	0.0	37.5	6
CTTTGCG	420	0.0	37.5	1
AGCTACG	150	0.0	37.5	9
GACGCAT	30	1.14002585E-4	37.499996	9
GCCGTCA	30	1.14002585E-4	37.499996	29
GACACGA	260	0.0	37.211536	25
>>END_MODULE