##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	98452
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02938487791005	33.0	31.0	34.0	30.0	34.0
2	32.184770243367325	34.0	31.0	34.0	30.0	34.0
3	32.32303051233088	34.0	31.0	34.0	30.0	34.0
4	35.89142932596595	37.0	35.0	37.0	35.0	37.0
5	35.88491853898346	37.0	35.0	37.0	35.0	37.0
6	35.81948563767115	37.0	35.0	37.0	35.0	37.0
7	35.892729451915656	37.0	35.0	37.0	35.0	37.0
8	35.6908341120546	37.0	35.0	37.0	35.0	37.0
9	37.50736399463698	39.0	37.0	39.0	35.0	39.0
10	36.986328363060174	39.0	37.0	39.0	33.0	39.0
11	36.96220493235282	39.0	37.0	39.0	33.0	39.0
12	37.07661601592654	39.0	37.0	39.0	33.0	39.0
13	37.059521391134766	39.0	37.0	39.0	33.0	39.0
14	38.18789867143379	40.0	37.0	41.0	33.0	41.0
15	38.197883232438144	40.0	37.0	41.0	33.0	41.0
16	38.25598261081542	40.0	38.0	41.0	33.0	41.0
17	38.22155974485028	40.0	37.0	41.0	33.0	41.0
18	38.03649494169748	39.0	37.0	41.0	33.0	41.0
19	37.80516393775647	39.0	37.0	41.0	34.0	41.0
20	37.468299272742044	39.0	35.0	41.0	33.0	41.0
21	37.30023767927518	39.0	35.0	40.0	33.0	41.0
22	37.310303498151384	39.0	35.0	40.0	33.0	41.0
23	37.23015276479909	39.0	35.0	40.0	33.0	41.0
24	37.12609190265307	39.0	35.0	40.0	33.0	41.0
25	37.04186811847398	39.0	35.0	40.0	32.0	41.0
26	36.96865477593142	39.0	35.0	40.0	32.0	41.0
27	36.90815829033438	39.0	35.0	40.0	32.0	41.0
28	36.79752569780197	38.0	35.0	40.0	32.0	41.0
29	36.71137203916629	38.0	35.0	40.0	32.0	41.0
30	36.6452484459432	38.0	35.0	40.0	31.0	41.0
31	36.41455734774306	38.0	35.0	40.0	31.0	41.0
32	36.109718441474016	38.0	35.0	40.0	30.0	41.0
33	35.82009507171007	38.0	35.0	40.0	29.0	41.0
34	35.53725673424613	39.0	35.0	40.0	25.0	41.0
35	35.30301060415228	39.0	35.0	40.0	24.0	41.0
36	35.19421647097062	38.0	35.0	40.0	23.0	41.0
37	35.01696258075001	38.0	35.0	40.0	23.0	41.0
38	34.933358387843825	38.0	35.0	40.0	23.0	41.0
39	34.800125949701375	38.0	35.0	40.0	22.0	41.0
40	34.6465485718929	38.0	34.0	40.0	21.0	41.0
41	34.49964449681063	38.0	34.0	40.0	20.0	41.0
42	34.35733149148824	38.0	34.0	40.0	19.0	41.0
43	34.23735424369236	38.0	34.0	40.0	18.0	41.0
44	34.179661154674356	38.0	34.0	40.0	18.0	41.0
45	34.18824401738919	38.0	34.0	40.0	19.0	41.0
46	33.965658391906715	38.0	33.0	40.0	18.0	41.0
47	33.77536261325316	37.0	33.0	40.0	18.0	41.0
48	33.70457684963231	37.0	33.0	40.0	18.0	41.0
49	33.74367204322919	37.0	33.0	40.0	18.0	41.0
50	33.69234753991793	37.0	33.0	40.0	18.0	41.0
51	32.63991589810263	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	3.0
14	2.0
15	9.0
16	6.0
17	22.0
18	35.0
19	63.0
20	118.0
21	196.0
22	321.0
23	455.0
24	775.0
25	1244.0
26	1901.0
27	2202.0
28	2284.0
29	2203.0
30	2243.0
31	2454.0
32	2964.0
33	3771.0
34	5493.0
35	7565.0
36	9828.0
37	12740.0
38	21049.0
39	18498.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.4347296144314	5.670783732174054	40.48673465241946	28.407752000975094
2	32.27257953114208	6.147158005931825	43.5440620810141	18.036200381912
3	19.440945841628405	6.026286921545525	54.863283630601714	19.669483606224354
4	15.971234713362856	5.4818591801080725	55.28988745784748	23.25701864868159
5	13.756957705277697	7.1435826595701455	55.62609190265307	23.47336773249909
6	18.17433876406777	8.734205501157925	59.297931987161256	13.793523747613051
7	69.09661560963718	3.381343192621785	22.769471417543574	4.752569780197457
8	68.46077276236136	4.304635761589404	21.366757404623574	5.867834071425669
9	62.861089668061595	6.2893592816804125	24.073660260837766	6.775890789420225
10	30.034940884898226	24.117336366960547	29.75155405679925	16.096168691341976
11	19.59127290456263	19.73245845691301	38.01040100759761	22.665867630926744
12	17.78937959614838	16.478080689066754	43.70353065453216	22.02900906025271
13	20.668955430057288	17.447080810953562	45.63340511112014	16.250558647869013
14	18.418112379636778	19.50595213911348	43.514606102466175	18.56132937878357
15	14.733067890951936	19.62987039369439	45.223052858245644	20.414008857108033
16	14.645715678706376	18.724860845894444	43.11847397716654	23.51094949823264
17	14.721894933571688	19.50595213911348	41.14187624426116	24.63027668305367
18	15.70105228944054	18.59789542111892	42.67866574574412	23.02238654369642
19	16.391744200219396	21.244870596839068	43.17738593426238	19.18599926867915
20	17.345508471133144	23.46727339210986	41.1997724779588	17.987445658798194
21	16.702555560069882	25.030471701946126	40.18811197334742	18.078860764636573
22	14.380611871775079	22.704465120058508	39.87628489009873	23.038638118067688
23	14.30138544671515	24.349937025149313	37.0403851623126	24.30829236582294
24	15.347580546865478	23.318977775972048	37.65794498842075	23.67549668874172
25	15.63807743875188	24.70036159752976	35.36545727867387	24.29610368504449
26	14.344045829439727	25.400195018892457	37.16430341689351	23.091455734774303
27	15.517206354365578	24.00154389956527	39.207938894080364	21.273310851988786
28	13.8057124283915	22.360134888067282	39.44257099906554	24.391581684475682
29	15.524316418153008	21.160565554788118	37.66708649900459	25.648031528054283
30	19.228659651403728	21.97314427335148	36.423841059602644	22.37435501564214
31	18.327712997196606	27.148254987201888	31.432576280827206	23.091455734774303
32	16.37142973225531	27.914110429447852	30.049161012473085	25.66529882582375
33	18.355137528948116	24.170153983667166	29.892739609149636	27.58196887823508
34	18.07784504123837	23.33624507374152	29.53520497298176	29.050704912038356
35	17.48974119367814	23.841059602649008	28.726689148011214	29.942510055661643
36	17.4805996830943	24.6607483849998	30.931824645512535	26.92682728639337
37	16.789907772315445	27.549465729492546	31.5046926420997	24.15593385609231
38	17.725389022061513	28.880063381140047	27.43976760248649	25.95477999431195
39	18.244423678543857	28.237110470076786	26.94409458416284	26.574371267216513
40	19.595335798155446	26.366147970584652	28.58245642546622	25.456059805793686
41	20.73091455734774	28.126396619672533	27.49563238938772	23.647056433592002
42	21.627798317962053	28.222890342501927	28.911550806484378	21.237760533051638
43	18.3510746353553	26.940031690570027	32.3253971478487	22.38349652622598
44	18.353106082151708	24.021858367529354	30.701255434120178	26.92378011619876
45	21.306829724129525	22.326616015926543	28.71450046723276	27.65205379271117
46	22.830414821435827	23.632836306017147	30.75712022102141	22.779628651525616
47	18.222077763783368	27.54337138910332	33.15829033437615	21.07626051273717
48	17.96611546743591	27.288424816154066	33.19282492991509	21.55263478649494
49	20.823345386584325	23.26209726567261	34.18010807296957	21.734449274773493
50	20.42315036769187	20.98890830049161	31.730183236501038	26.857758095315482
51	18.732986633080078	20.51964409052127	30.161906309673746	30.5854629667249
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	299.0
1	485.5
2	672.0
3	2767.5
4	4863.0
5	3091.0
6	1319.0
7	1295.0
8	1271.0
9	1256.0
10	1241.0
11	1186.5
12	1132.0
13	1072.5
14	1013.0
15	951.0
16	889.0
17	798.5
18	708.0
19	720.5
20	733.0
21	697.0
22	661.0
23	663.5
24	666.0
25	639.0
26	709.0
27	806.0
28	857.5
29	909.0
30	1017.0
31	1125.0
32	1174.5
33	1224.0
34	1417.0
35	1610.0
36	1716.0
37	1822.0
38	2025.0
39	2228.0
40	2532.0
41	2836.0
42	3367.0
43	3898.0
44	4635.5
45	5373.0
46	6973.5
47	8574.0
48	10244.0
49	11914.0
50	11782.0
51	11650.0
52	10010.0
53	8370.0
54	6714.5
55	5059.0
56	4184.0
57	3309.0
58	2973.0
59	2637.0
60	2453.5
61	2270.0
62	2143.5
63	2017.0
64	1776.5
65	1536.0
66	1337.5
67	1139.0
68	939.5
69	740.0
70	628.5
71	517.0
72	436.0
73	355.0
74	280.5
75	161.5
76	117.0
77	91.5
78	66.0
79	48.0
80	30.0
81	25.0
82	20.0
83	15.0
84	10.0
85	6.5
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	98452.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.08454881566652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91910864944934	49.15491813269411
2	12.524956527339473	15.802624629260958
3	4.688606942744896	8.873359606711901
4	1.9949120886198237	5.0339251615000205
5	0.9692793198943774	3.057327428594645
6	0.5184517292458298	1.9623776053305164
7	0.3349004959103497	1.4788932677853168
8	0.23829458362851805	1.2026165034737741
9	0.13363817865653377	0.7587453784585382
>10	0.6392091195981194	6.814488278551985
>50	0.017711083918335802	0.7211636127249827
>100	0.01932118245636633	2.0791857961239995
>500	0.0	0.0
>1k	0.0016100985380305274	3.0603745987892577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3013	3.0603745987892577	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	272	0.2762767643115427	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	269	0.2732295941169301	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	254	0.257993743143867	Illumina Single End Adapter 2 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.20111323284443178	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	191	0.1940031690570024	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	166	0.16861008410189737	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143	0.14524844594320074	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	117	0.11883963758989152	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	116	0.11782391419168732	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	112	0.11376102059887051	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.10868240360784952	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	102	0.1036037866168285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2976069556738309	0.0
2	0.0	0.0	0.0	0.9943932068419128	0.0
3	0.0	0.0	0.0	1.3712265875756713	0.0
4	0.0	0.0	0.0	2.117783285255759	0.0
5	0.0	0.0	0.0	3.748019339373502	0.0
6	0.0	0.0	0.0	5.364035265916385	0.0
7	0.0	0.0	0.0	6.760654938447162	0.0
8	0.0	0.0	0.0	8.813431926217852	0.0
9	0.0	0.0	0.0	9.7682119205298	0.0
10	0.0	0.0	0.0	11.993661885995206	0.0
11	0.0	0.0	0.0	17.09665623857311	0.0
12	0.0	0.0	0.0	22.085889570552148	0.0
13	0.0	0.0	0.0	23.598301710478204	0.0
14	0.0	0.0	0.0	24.1051476861821	0.0
15	0.0	0.0	0.0	24.785682362978914	0.0
16	0.0	0.0	0.0	26.433185714866127	0.0
17	0.0	0.0	0.0	28.53471742575062	0.0
18	0.0	0.0	0.0	30.562101328566204	0.0
19	0.0	0.0	0.0	31.82058261894121	0.0
20	0.0	0.0	0.0	32.89318652744485	0.0
21	0.0	0.0	0.0	34.23902003006541	0.0
22	0.0	0.0	0.0	35.42233778897331	0.0
23	0.0	0.0	0.0	36.27960833705765	0.0
24	0.0	0.0	0.0	36.79559582334539	0.0
25	0.0	0.0	0.0	37.09421850241742	0.0
26	0.0	0.0	0.0	37.35221224556129	0.0
27	0.0	0.0	0.0	37.571608499573394	0.0
28	0.001015723398204201	0.0	0.0	37.77780034940885	0.0
29	0.001015723398204201	0.0	0.0	38.01141673099581	0.0
30	0.001015723398204201	0.0	0.0	38.26331613375046	0.0
31	0.001015723398204201	0.0	0.0	38.44005200503799	0.0
32	0.001015723398204201	0.0	0.0	38.62288221671474	0.0
33	0.001015723398204201	0.0	0.0	38.782350790232805	0.0
34	0.001015723398204201	0.0	0.0	38.90931621500833	0.0
35	0.001015723398204201	0.0	0.0	39.08097346930484	0.0
36	0.001015723398204201	0.0	0.0	39.23028480884086	0.0
37	0.001015723398204201	0.0	0.0	39.40702068012839	0.0
38	0.001015723398204201	0.0	0.0	39.56953642384106	0.0
39	0.001015723398204201	0.0	0.0	39.73103644415553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	20	7.001866E-4	45.000004	14
AATCCTA	20	7.001866E-4	45.000004	13
GCTGGGT	20	7.001866E-4	45.000004	4
GACGTGG	20	7.001866E-4	45.000004	25
TATGGGA	30	2.145267E-6	45.000004	4
ATCAGAC	20	7.001866E-4	45.000004	21
CGACCTC	20	7.001866E-4	45.000004	12
TTCCGAT	20	7.001866E-4	45.000004	13
CTTGCGG	20	7.001866E-4	45.000004	2
TCAGACG	20	7.001866E-4	45.000004	22
TAGGGCC	20	7.001866E-4	45.000004	5
TCGAGCT	30	2.145267E-6	45.000004	16
TCGCATT	25	3.8647864E-5	45.0	22
CTCCGGG	25	3.8647864E-5	45.0	3
GCGCGAC	25	3.8647864E-5	45.0	9
CGCATTT	25	3.8647864E-5	45.0	23
AAATTCT	25	3.8647864E-5	45.0	33
CGGGAGT	25	3.8647864E-5	45.0	6
CGGGAGC	25	3.8647864E-5	45.0	6
AATTCTA	25	3.8647864E-5	45.0	34
>>END_MODULE