SRR2933658.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933658.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89048
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3927	4.40998113377055	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	523	0.587323690593837	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	488	0.5480190459078249	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	376	0.4222441829125865	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	263	0.29534633006917616	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	223	0.25042673614230526	RNA PCR Primer, Index 24 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	204	0.22908992902704162	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	199	0.22347497978618272	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	171	0.1920312640373731	RNA PCR Primer, Index 24 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.14711167011050222	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	102	0.11454496451352081	TruSeq Adapter, Index 3 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.11342197466534902	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	20	6.998225E-4	45.0	26
TCAAGCG	20	6.998225E-4	45.0	17
GCGATTG	20	6.998225E-4	45.0	9
TTCGCGG	20	6.998225E-4	45.0	2
TGTCTTG	35	1.1951124E-7	45.0	1
TCGCGGG	20	6.998225E-4	45.0	3
GGCGATT	35	1.1951124E-7	45.0	8
ATGGTCA	20	6.998225E-4	45.0	13
GGTCAAG	20	6.998225E-4	45.0	15
GCTACGA	20	6.998225E-4	45.0	10
GACACGA	20	6.998225E-4	45.0	25
TTGGGCA	40	6.693881E-9	45.0	5
CGGGATT	20	6.998225E-4	45.0	6
GACCGAT	40	6.693881E-9	45.0	9
TACGAAT	20	6.998225E-4	45.0	12
CTACGAA	20	6.998225E-4	45.0	11
TGGGCAC	40	6.693881E-9	45.0	6
TGGGTAT	20	6.998225E-4	45.0	6
ACGACCA	20	6.998225E-4	45.0	28
GGGAACA	40	6.693881E-9	45.0	7