##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933658.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89048
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0983402210044	33.0	31.0	34.0	30.0	34.0
2	32.27841164315875	34.0	31.0	34.0	30.0	34.0
3	32.379424580001796	34.0	31.0	34.0	30.0	34.0
4	35.93266552870362	37.0	35.0	37.0	35.0	37.0
5	35.948230167999284	37.0	35.0	37.0	35.0	37.0
6	35.905354415596086	37.0	35.0	37.0	35.0	37.0
7	35.94104303297098	37.0	35.0	37.0	35.0	37.0
8	35.75960156320187	37.0	35.0	37.0	35.0	37.0
9	37.59223115623035	39.0	37.0	39.0	35.0	39.0
10	37.13969993711257	39.0	37.0	39.0	33.0	39.0
11	37.14924535082203	39.0	37.0	39.0	34.0	39.0
12	37.191660677387475	39.0	37.0	39.0	34.0	39.0
13	37.17515272661935	39.0	37.0	39.0	33.0	39.0
14	38.35625729943401	40.0	38.0	41.0	34.0	41.0
15	38.37599946096487	40.0	38.0	41.0	34.0	41.0
16	38.414798760219206	40.0	38.0	41.0	34.0	41.0
17	38.342803881052916	40.0	38.0	41.0	33.0	41.0
18	38.02033734615039	39.0	37.0	40.0	34.0	41.0
19	37.68959437606684	39.0	37.0	40.0	34.0	41.0
20	37.27793998742251	39.0	35.0	40.0	33.0	41.0
21	37.1654164046357	39.0	35.0	40.0	33.0	41.0
22	37.14409082741892	39.0	35.0	40.0	33.0	41.0
23	37.063505075914115	38.0	35.0	40.0	33.0	41.0
24	36.964580900188665	38.0	35.0	40.0	33.0	41.0
25	36.86098508669482	38.0	35.0	40.0	33.0	41.0
26	36.76058979426826	38.0	35.0	40.0	32.0	41.0
27	36.664372024076904	38.0	35.0	40.0	32.0	41.0
28	36.52128065762285	38.0	35.0	40.0	32.0	41.0
29	36.40882445422693	38.0	35.0	40.0	31.0	41.0
30	36.223733267451266	38.0	35.0	40.0	31.0	41.0
31	35.91275491869553	38.0	35.0	40.0	30.0	41.0
32	35.507187135028296	38.0	35.0	40.0	27.0	41.0
33	35.12008130446501	38.0	35.0	40.0	24.0	41.0
34	34.713210852573894	38.0	35.0	40.0	21.0	41.0
35	34.44111041236187	38.0	34.0	40.0	18.0	41.0
36	34.22219477135927	38.0	34.0	40.0	18.0	41.0
37	33.976282454406615	38.0	34.0	40.0	17.0	41.0
38	33.87430374629413	38.0	34.0	40.0	16.0	41.0
39	33.83195579911958	38.0	33.0	40.0	15.0	41.0
40	33.69259275896146	38.0	33.0	40.0	15.0	41.0
41	33.56976012936843	38.0	33.0	40.0	15.0	41.0
42	33.45738253526188	38.0	33.0	40.0	15.0	41.0
43	33.29998427814213	37.0	33.0	40.0	14.0	41.0
44	33.16939178869823	37.0	33.0	40.0	12.0	41.0
45	33.176163417482705	37.0	33.0	40.0	12.0	41.0
46	32.984996855628424	37.0	32.0	40.0	12.0	41.0
47	32.81033824454227	37.0	32.0	40.0	11.0	41.0
48	32.762386578025335	37.0	32.0	40.0	10.0	41.0
49	32.80612703261163	37.0	32.0	40.0	10.0	41.0
50	32.72273380648639	37.0	32.0	40.0	10.0	41.0
51	31.69660183271943	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	6.0
15	6.0
16	12.0
17	15.0
18	41.0
19	59.0
20	132.0
21	230.0
22	364.0
23	502.0
24	839.0
25	1401.0
26	2160.0
27	2633.0
28	2443.0
29	2304.0
30	2341.0
31	2643.0
32	2734.0
33	3335.0
34	5010.0
35	6826.0
36	8674.0
37	10990.0
38	17737.0
39	15594.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.05610457281466	5.490297367711796	39.51464378762016	31.938954271853383
2	36.74871979157309	5.169122271134669	40.58260713323151	17.499550804060732
3	19.230078160093434	5.151154433563921	57.573443536070435	18.045323870272213
4	15.176084808193336	5.571152636780163	57.7778276884377	21.474934866588807
5	12.948072949420537	6.63911598239152	57.986703800197645	22.426107267990297
6	17.052600844488367	7.685742520887612	62.11144551253257	13.150211122091457
7	63.172670919054895	3.1567244632108524	28.842871260443804	4.82773335729045
8	62.122675411014285	3.7653849609199534	28.151109513969995	5.960830114095769
9	56.78510466265385	5.605965322073488	30.943985266373193	6.664944748899471
10	28.303836133321354	19.497349743958313	36.508399964064324	15.690414158656004
11	19.158206809810437	18.121687179947894	41.329395382265744	21.390710627975924
12	17.35805408319109	15.176084808193336	47.48001078070254	19.985850327913035
13	20.006064145180126	16.043032970981944	48.56257299434013	15.388329889497799
14	17.598373910699845	18.17671368250831	46.84889048603	17.376021920761836
15	13.92507411732998	17.47484502740095	48.53337525828766	20.066705596981404
16	14.327104482975475	16.640463570209324	46.73659150121283	22.295840445602373
17	14.05309496002156	17.310888509567874	44.39403467792651	24.241981852484056
18	15.207528523942143	16.48436798131345	46.93985266373192	21.368250831012485
19	15.473677117958854	18.90553409397179	47.09257928308328	18.528209504986076
20	16.37656095588896	20.83819962267541	44.70173389632558	18.083505525110052
21	16.414742610726798	21.786003054532387	44.63435450543527	17.164899829305543
22	13.633096756805319	20.710178779983828	43.55628425119037	22.100440212020484
23	14.023897223969096	21.049321714131704	41.504581798580546	23.42219926331866
24	15.26929296559159	20.433923277333573	41.82575689515767	22.471026861917167
25	14.578654208965952	22.038675770371036	40.030096127931	23.35257389273201
26	13.618497888779086	22.678779983828946	42.18848261611715	21.51423951127482
27	15.632018686551074	21.796109963165932	43.3047345251999	19.2671368250831
28	13.712829036025514	19.94991465277154	44.26039888599407	22.076857425208875
29	16.387790854370678	18.4159105201689	42.469230078160095	22.727068547300334
30	20.22280118587728	19.751145449645136	40.31982750875932	19.706225855718266
31	19.29858054083191	24.39583146168359	37.14401221812955	19.161575779354955
32	18.174467702811967	23.63107537507861	37.24283532476866	20.95162159734076
33	21.529961369149223	20.358682957506062	34.39381007995688	23.717545593387836
34	19.047030814841435	20.47098194232324	36.81385320276705	23.668134040068278
35	19.98921929745755	20.240769023448028	37.30347677656994	22.466534902524483
36	23.500808552690682	21.921884826161172	36.19171682687988	18.38558979426826
37	23.405354415596083	22.64845925792831	36.62968286766687	17.316503458808732
38	23.241397897763004	21.627661485940166	35.29557092803881	19.835369688258016
39	23.926421705147785	20.536115353517204	32.03890036834067	23.49856257299434
40	20.25424490162609	22.649582247776483	35.070972958404454	22.025199892192976
41	19.610771718623663	23.560327014643786	35.78519450184171	21.043706764890846
42	23.768080136555568	20.391249663103046	37.35738028928218	18.483289911059202
43	24.843904411104123	18.275536789147427	37.766148594016705	19.11441020573174
44	22.127391968376607	18.652861378133142	35.39888599407061	23.820860659419637
45	22.92246878088222	18.264306890665708	33.70317132333124	25.110053005120836
46	23.041505704788428	19.94879166292337	37.20802263947534	19.801679992812865
47	19.268259814931273	22.696747821399697	39.59886802623304	18.436124337435988
48	19.459168089120475	20.961728505974307	40.04357200610906	19.535531398796156
49	22.65744317671368	17.676983200071874	39.386622944928575	20.278950678285867
50	21.461458988410744	17.03575599676579	36.527490791483245	24.97529422334022
51	19.113287215883567	16.988590423142576	34.91150839996406	28.986613961009795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	362.0
1	655.0
2	948.0
3	3755.0
4	6562.0
5	4105.5
6	1649.0
7	1583.0
8	1517.0
9	1430.5
10	1344.0
11	1300.5
12	1257.0
13	1159.5
14	1062.0
15	982.5
16	903.0
17	851.0
18	799.0
19	748.5
20	698.0
21	659.5
22	621.0
23	590.0
24	559.0
25	572.0
26	606.5
27	628.0
28	625.0
29	622.0
30	675.0
31	728.0
32	776.5
33	825.0
34	956.5
35	1088.0
36	1211.5
37	1335.0
38	1507.5
39	1680.0
40	2059.0
41	2438.0
42	2976.5
43	3515.0
44	3989.5
45	4464.0
46	5735.0
47	7006.0
48	8659.0
49	10312.0
50	10355.0
51	10398.0
52	8698.5
53	6999.0
54	5468.5
55	3938.0
56	3465.5
57	2993.0
58	2701.0
59	2409.0
60	2303.5
61	2198.0
62	1990.5
63	1783.0
64	1684.5
65	1586.0
66	1336.5
67	1087.0
68	926.0
69	765.0
70	633.0
71	501.0
72	445.5
73	390.0
74	298.0
75	171.0
76	136.0
77	112.0
78	88.0
79	57.5
80	27.0
81	26.5
82	26.0
83	17.5
84	9.0
85	5.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	89048.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.46127931003503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68360142273185	50.56823286317492
2	12.017129408433757	15.252448117869013
3	4.331899984073897	8.247237444973496
4	1.6439277308842526	4.1730302758063065
5	0.7909964431703562	2.5098823106639117
6	0.3839960361699492	1.4621327823196477
7	0.21765673939586985	0.9668942592758961
8	0.15749146183928792	0.799568771898302
9	0.12917839004795525	0.7378043302488545
>10	0.5945745076179859	6.572859581349384
>50	0.028313071791332658	1.1768933608840177
>100	0.017695669869582913	2.5357110771718627
>500	0.0017695669869582911	0.587323690593837
>1k	0.0017695669869582911	4.40998113377055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3927	4.40998113377055	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	523	0.587323690593837	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	488	0.5480190459078249	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	376	0.4222441829125865	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	263	0.29534633006917616	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	223	0.25042673614230526	RNA PCR Primer, Index 24 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	204	0.22908992902704162	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	199	0.22347497978618272	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	171	0.1920312640373731	RNA PCR Primer, Index 24 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.14711167011050222	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	102	0.11454496451352081	TruSeq Adapter, Index 3 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.11342197466534902	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4447039798760219	0.0
2	0.0	0.0	0.0	1.555340939717905	0.0
3	0.0	0.0	0.0	2.1246967927409934	0.0
4	0.0	0.0	0.0	2.86362411283802	0.0
5	0.0	0.0	0.0	4.6379480729494205	0.0
6	0.0	0.0	0.0	6.161845296918516	0.0
7	0.0	0.0	0.0	7.400503099451981	0.0
8	0.0	0.0	0.0	9.28824903422873	0.0
9	0.0	0.0	0.0	10.07995687718983	0.0
10	0.0	0.0	0.0	12.105830563291708	0.0
11	0.0	0.0	0.0	16.94816278860839	0.0
12	0.0	0.0	0.0	21.24921390710628	0.0
13	0.0	0.0	0.0	22.638352349294763	0.0
14	0.0	0.0	0.0	23.199847273380648	0.0
15	0.0	0.0	0.0	23.964603359985627	0.0
16	0.0	0.0	0.0	25.874809091725812	0.0
17	0.0	0.0	0.0	28.125280747462043	0.0
18	0.0	0.0	0.0	30.15676938280478	0.0
19	0.0	0.0	0.0	31.39205821579373	0.0
20	0.0	0.0	0.0	32.4555296020124	0.0
21	0.0	0.0	0.0	33.69194142484952	0.0
22	0.0	0.0	0.0	34.77674961818345	0.0
23	0.0	0.0	0.0	35.57519540023358	0.0
24	0.0	0.0	0.0	36.050220106010244	0.0
25	0.0	0.0	0.0	36.371395202587365	0.0
26	0.0	0.0	0.0	36.60946905039978	0.0
27	0.0	0.0	0.0	36.823960111400595	0.0
28	0.0	0.0	0.0	37.00476147695625	0.0
29	0.0	0.0	0.0	37.18219387296739	0.0
30	0.0	0.0	0.0	37.39331596442368	0.0
31	0.0	0.0	0.0	37.55053454316773	0.0
32	0.0	0.0	0.0	37.711122091456296	0.0
33	0.0	0.0	0.0	37.88630850777109	0.0
34	0.0	0.0	0.0	38.02668223879256	0.0
35	0.0	0.0	0.0	38.19176174647381	0.0
36	0.0	0.0	0.0	38.35010331506603	0.0
37	0.0	0.0	0.0	38.50844488365825	0.0
38	0.0	0.0	0.0	38.66678645225047	0.0
39	0.0	0.0	0.0	38.81951307160183	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	20	6.998225E-4	45.0	26
TCAAGCG	20	6.998225E-4	45.0	17
GCGATTG	20	6.998225E-4	45.0	9
TTCGCGG	20	6.998225E-4	45.0	2
TGTCTTG	35	1.1951124E-7	45.0	1
TCGCGGG	20	6.998225E-4	45.0	3
GGCGATT	35	1.1951124E-7	45.0	8
ATGGTCA	20	6.998225E-4	45.0	13
GGTCAAG	20	6.998225E-4	45.0	15
GCTACGA	20	6.998225E-4	45.0	10
GACACGA	20	6.998225E-4	45.0	25
TTGGGCA	40	6.693881E-9	45.0	5
CGGGATT	20	6.998225E-4	45.0	6
GACCGAT	40	6.693881E-9	45.0	9
TACGAAT	20	6.998225E-4	45.0	12
CTACGAA	20	6.998225E-4	45.0	11
TGGGCAC	40	6.693881E-9	45.0	6
TGGGTAT	20	6.998225E-4	45.0	6
ACGACCA	20	6.998225E-4	45.0	28
GGGAACA	40	6.693881E-9	45.0	7
>>END_MODULE