##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933657.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90196
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02990154774048	33.0	31.0	34.0	30.0	34.0
2	32.21333540290035	34.0	31.0	34.0	30.0	34.0
3	32.32280810678966	34.0	31.0	34.0	30.0	34.0
4	35.84925052108741	37.0	35.0	37.0	35.0	37.0
5	35.905661004922614	37.0	35.0	37.0	35.0	37.0
6	35.87292119384452	37.0	35.0	37.0	35.0	37.0
7	35.873386846423344	37.0	35.0	37.0	35.0	37.0
8	35.71234866291188	37.0	35.0	37.0	35.0	37.0
9	37.56004700873653	39.0	37.0	39.0	35.0	39.0
10	37.1974699543217	39.0	37.0	39.0	34.0	39.0
11	37.18958712138011	39.0	37.0	39.0	34.0	39.0
12	37.19555190917557	39.0	37.0	39.0	34.0	39.0
13	37.19735908466007	39.0	37.0	39.0	34.0	39.0
14	38.39588230076722	40.0	38.0	41.0	34.0	41.0
15	38.420129495764776	40.0	38.0	41.0	34.0	41.0
16	38.474355847265954	40.0	38.0	41.0	34.0	41.0
17	38.453778438068206	40.0	38.0	41.0	34.0	41.0
18	38.17822298106346	39.0	38.0	41.0	34.0	41.0
19	37.88245598474433	39.0	37.0	41.0	34.0	41.0
20	37.44039646990998	39.0	35.0	41.0	34.0	41.0
21	37.36425118630538	39.0	35.0	40.0	33.0	41.0
22	37.31122222714976	39.0	35.0	40.0	33.0	41.0
23	37.19871169453191	39.0	35.0	40.0	33.0	41.0
24	37.08371768149364	39.0	35.0	40.0	33.0	41.0
25	36.988225641935344	38.0	35.0	40.0	33.0	41.0
26	36.94638343163777	38.0	35.0	40.0	33.0	41.0
27	36.83742072819194	38.0	35.0	40.0	33.0	41.0
28	36.76603175307109	38.0	35.0	40.0	33.0	41.0
29	36.594516386535986	38.0	35.0	40.0	32.0	41.0
30	36.37213401924697	38.0	35.0	40.0	31.0	41.0
31	36.02862654663178	38.0	35.0	40.0	30.0	41.0
32	35.74085325291587	38.0	35.0	40.0	29.0	41.0
33	35.28043372211628	38.0	35.0	40.0	24.0	41.0
34	34.884130116634886	38.0	35.0	40.0	21.0	41.0
35	34.64488447381259	38.0	35.0	40.0	20.0	41.0
36	34.39312164619274	38.0	34.0	40.0	18.0	41.0
37	34.17598341389862	38.0	34.0	40.0	18.0	41.0
38	34.05325069847887	38.0	34.0	40.0	17.0	41.0
39	34.08795290256774	38.0	34.0	40.0	17.0	41.0
40	33.91521796975476	38.0	34.0	40.0	15.0	41.0
41	33.84494877821633	38.0	33.0	40.0	15.0	41.0
42	33.65109317486363	38.0	33.0	40.0	15.0	41.0
43	33.4929486895206	38.0	33.0	40.0	15.0	41.0
44	33.330690939731255	37.0	33.0	40.0	13.0	41.0
45	33.30084482682159	37.0	33.0	40.0	12.0	41.0
46	33.120814670273624	37.0	33.0	40.0	12.0	41.0
47	33.04883808594616	37.0	33.0	40.0	12.0	41.0
48	32.97737150206218	37.0	32.0	40.0	12.0	41.0
49	32.96951084305291	37.0	32.0	40.0	11.0	41.0
50	32.82822963324316	37.0	32.0	40.0	10.0	41.0
51	31.983679985808685	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	4.0
13	7.0
14	8.0
15	8.0
16	5.0
17	20.0
18	46.0
19	89.0
20	113.0
21	205.0
22	337.0
23	505.0
24	837.0
25	1376.0
26	2105.0
27	2630.0
28	2518.0
29	2347.0
30	2237.0
31	2384.0
32	2644.0
33	3254.0
34	4955.0
35	6849.0
36	8536.0
37	10351.0
38	17635.0
39	18179.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.462237793250253	5.5656570136147945	39.27557763093707	31.69652756219788
2	36.417357754224135	5.213091489644774	40.622644019690455	17.74690673644064
3	19.36782118940973	5.266308927225154	57.14000620870105	18.225863674664065
4	15.35101334870726	5.668765798926781	57.4338108120094	21.546410040356555
5	12.894141647079692	6.91050600913566	57.820745931083415	22.37460641270123
6	17.343341168122755	7.7775067630493595	61.54707525832631	13.332076810501576
7	63.1812940706905	3.2628941416470796	28.48241607166615	5.073395715996274
8	62.17238014989578	3.86713379750765	27.834937247771517	6.125548804825048
9	57.03024524369151	5.636613597055302	30.48693955386048	6.8462016053927
10	28.32054636569249	19.50197347997694	36.12909663399707	16.048383520333495
11	19.139429686460595	18.364450751696307	40.92531819592887	21.57080136591423
12	17.627167501884784	15.05166526231762	47.42338906381658	19.89777817198102
13	19.993126080979202	16.29784025899153	48.29925939066034	15.409774269368931
14	17.787928511242185	18.23695064082664	46.53310568096146	17.44201516696971
15	13.931881679897112	17.716971927801676	48.119650538826555	20.231495853474655
16	14.608186615814448	16.645970996496516	46.284757638919686	22.46108474876935
17	14.369816843318993	17.423167324493324	44.0629296199388	24.14408621224888
18	15.274513282185461	16.7834493769125	46.58299702869307	21.35904031220897
19	15.659231008026964	19.06958179963635	46.53643177081024	18.734755421526454
20	16.706949310390705	20.880083373985542	44.514169142755776	17.898798172867977
21	16.60827531154375	21.712714532795246	44.45651691871036	17.22249323695064
22	14.050512217836712	20.814670273626326	42.715863231185416	22.418954277351546
23	14.47403432524724	21.115127056632225	41.04949221694975	23.361346401170785
24	15.904252960219967	20.67940928644286	40.98851390305557	22.42782385028161
25	14.664730143243604	22.0142800124174	39.77892589471817	23.542063949620825
26	13.880881635549247	22.793693733646727	41.760166747971084	21.56525788283294
27	15.948600824870281	21.96327996806954	42.760211095835736	19.327908111224446
28	13.931881679897112	19.9509956095614	43.8256685440596	22.291454166481884
29	16.67147101867045	18.661581444853432	42.110514878708585	22.556432657767527
30	20.480952592132688	19.915517317841147	39.619273581977026	19.984256508049135
31	19.4265821100714	24.69510843052907	36.66903188611468	19.209277573284847
32	18.42210297574172	23.13738968468668	36.86748858042485	21.57301875914675
33	21.964388664685796	20.180495809126793	34.216594970952144	23.638520555235264
34	19.486451727349326	20.529735243248037	36.48277085458335	23.501042174819283
35	20.168300146347953	20.302452436915164	37.109184442769084	22.420062973967802
36	23.644064038316557	21.847975519978714	36.078096589649206	18.429863852055522
37	23.680651026653067	22.34134551421349	36.78322763758925	17.19477582154419
38	23.39571599627478	21.856845092908777	34.76983458246485	19.97760432835159
39	23.77378154241873	20.550800478956937	32.24200629739678	23.433411681227547
40	20.46543083950508	22.92895472083019	34.5447691693645	22.060845270300238
41	19.73147367954233	23.57089006164353	35.54037873076412	21.157257528050025
42	23.5875205108874	20.606235309769836	37.16351057696572	18.642733602377046
43	24.7971085192248	18.3777551110914	37.4273803716351	19.397755998048694
44	22.289236773249367	18.671559714399752	35.21553062220054	23.823672890150338
45	22.942259080225288	18.542950906913834	33.772007627832714	24.74278238502816
46	23.297041997427822	20.123952281697637	36.814271142844476	19.76473457803007
47	19.143864472925628	23.15845492039558	39.26559936139075	18.43208124528804
48	19.296864605969223	21.080757461528226	39.88203468003015	19.740343252472393
49	22.841367688145816	17.979733025854806	38.72122932280811	20.45766996319127
50	21.38897512084793	17.170384495986518	36.38187946250388	25.058760920661673
51	19.54410395139474	17.069493103907046	34.47935606900528	28.907046875692938
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	677.0
2	974.0
3	3690.0
4	6406.0
5	4059.5
6	1713.0
7	1572.0
8	1431.0
9	1382.5
10	1334.0
11	1298.5
12	1263.0
13	1170.5
14	1078.0
15	986.5
16	895.0
17	861.5
18	828.0
19	758.0
20	688.0
21	653.0
22	618.0
23	599.5
24	581.0
25	601.5
26	626.5
27	631.0
28	608.5
29	586.0
30	671.5
31	757.0
32	832.0
33	907.0
34	1013.0
35	1119.0
36	1209.0
37	1299.0
38	1532.5
39	1766.0
40	2115.5
41	2465.0
42	3077.5
43	3690.0
44	4145.5
45	4601.0
46	5723.0
47	6845.0
48	8576.0
49	10307.0
50	10371.5
51	10436.0
52	8669.5
53	6903.0
54	5466.0
55	4029.0
56	3558.0
57	3087.0
58	2808.5
59	2530.0
60	2446.5
61	2363.0
62	2156.0
63	1949.0
64	1791.0
65	1633.0
66	1383.0
67	1133.0
68	963.5
69	794.0
70	695.0
71	596.0
72	517.0
73	438.0
74	327.5
75	176.0
76	135.0
77	111.5
78	88.0
79	67.0
80	46.0
81	34.0
82	22.0
83	14.0
84	6.0
85	6.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	90196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.794403299481125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7115050399722	50.851479001286094
2	11.993395898505387	15.302230697591913
3	4.304831421619743	8.238724555412656
4	1.7431352102884952	4.448090824426804
5	0.7160236357316649	2.28391502949133
6	0.413625304136253	1.58321876801632
7	0.23635731664928744	1.0554791786775466
8	0.18074383037886688	0.9224355847265954
9	0.10775112964893988	0.6186527118719234
>10	0.5474452554744526	6.225331500288261
>50	0.026068821689259645	1.143066211361923
>100	0.013903371567605143	1.944653864916404
>500	0.0034758428919012857	1.2140227948024302
>1k	0.0017379214459506429	4.1686992771298055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3760	4.1686992771298055	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	550	0.60978313894186	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	545	0.6042396558605703	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	408	0.45234821943323433	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	268	0.2971306931571245	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	232	0.2572176149718391	RNA PCR Primer, Index 24 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.23836977249545435	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	196	0.21730453678655373	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	183	0.2028914807752007	RNA PCR Primer, Index 24 (95% over 23bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140	0.1552175262761098	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	112	0.12417402102088784	TruSeq Adapter, Index 3 (95% over 22bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98	0.10865226839327687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.47452215175839285	0.0
2	0.0	0.0	0.0	1.6231318462016053	0.0
3	0.0011086966162579272	0.0	0.0	2.183023637411859	0.0
4	0.0011086966162579272	0.0	0.0	2.95245908909486	0.0
5	0.0011086966162579272	0.0	0.0	4.65874318151581	0.0
6	0.0011086966162579272	0.0	0.0	6.0180052330480285	0.0
7	0.0011086966162579272	0.0	0.0	7.258636746640649	0.0
8	0.0011086966162579272	0.0	0.0	9.07689919730365	0.0
9	0.0011086966162579272	0.0	0.0	9.861856401614263	0.0
10	0.0011086966162579272	0.0	0.0	11.912945141691427	0.0
11	0.0011086966162579272	0.0	0.0	16.7413189054947	0.0
12	0.0011086966162579272	0.0	0.0	20.921105148787085	0.0
13	0.0011086966162579272	0.0	0.0	22.290345469865628	0.0
14	0.0011086966162579272	0.0	0.0	22.75378065546144	0.0
15	0.0011086966162579272	0.0	0.0	23.504368264668056	0.0
16	0.0011086966162579272	0.0	0.0	25.33039159164486	0.0
17	0.0011086966162579272	0.0	0.0	27.473502150871436	0.0
18	0.0011086966162579272	0.0	0.0	29.517938711251052	0.0
19	0.0011086966162579272	0.0	0.0	30.662113619229235	0.0
20	0.0011086966162579272	0.0	0.0	31.62667967537363	0.0
21	0.0011086966162579272	0.0	0.0	32.763093707038	0.0
22	0.0011086966162579272	0.0	0.0	33.779768504146524	0.0
23	0.0011086966162579272	0.0	0.0	34.438334294203734	0.0
24	0.0011086966162579272	0.0	0.0	34.899552086567034	0.0
25	0.0011086966162579272	0.0	0.0	35.252117610537056	0.0
26	0.0011086966162579272	0.0	0.0	35.45057430484722	0.0
27	0.0011086966162579272	0.0	0.0	35.652357089006166	0.0
28	0.0011086966162579272	0.0	0.0	35.82863985099117	0.0
29	0.0011086966162579272	0.0	0.0	36.01046609605748	0.0
30	0.0011086966162579272	0.0	0.0	36.20116191405384	0.0
31	0.0011086966162579272	0.0	0.0	36.32755332830724	0.0
32	0.0011086966162579272	0.0	0.0	36.49718391059471	0.0
33	0.0011086966162579272	0.0	0.0	36.679010155661004	0.0
34	0.0011086966162579272	0.0	0.0	36.84420595148344	0.0
35	0.0011086966162579272	0.0	0.0	36.978358242050646	0.0
36	0.0011086966162579272	0.0	0.0	37.10585835292031	0.0
37	0.0011086966162579272	0.0	0.0	37.24777151980132	0.0
38	0.0011086966162579272	0.0	0.0	37.41629340547253	0.0
39	0.0011086966162579272	0.0	0.0	37.56375005543483	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTAG	20	6.99871E-4	45.0	1
ACACGAC	25	3.8621743E-5	45.0	26
GCGACCT	25	3.8621743E-5	45.0	11
TATGGGA	25	3.8621743E-5	45.0	4
GGTCAAG	25	3.8621743E-5	45.0	15
CACGACC	25	3.8621743E-5	45.0	27
TGAAACA	20	6.99871E-4	45.0	38
GGGAATC	25	3.8621743E-5	45.0	7
GGGCGCG	20	6.99871E-4	45.0	7
CCTCAGC	20	6.99871E-4	45.0	16
GCGGGAT	20	6.99871E-4	45.0	5
AAACACG	20	6.99871E-4	45.0	40
TAGGGAT	35	1.1953489E-7	45.0	5
AGACACG	25	3.8621743E-5	45.0	24
CGAGACA	25	3.8621743E-5	45.0	22
CCGCGGG	25	3.8621743E-5	45.0	3
AGGGCAC	20	6.99871E-4	45.0	11
TCAAGCG	25	3.8621743E-5	45.0	17
GCTCCAC	20	6.99871E-4	45.0	12
ACGACCA	25	3.8621743E-5	45.0	28
>>END_MODULE