##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933656.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452709
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35113505585266	33.0	31.0	34.0	30.0	34.0
2	32.53835907834834	34.0	31.0	34.0	31.0	34.0
3	32.543072923224415	34.0	31.0	34.0	31.0	34.0
4	36.13164306430842	37.0	35.0	37.0	35.0	37.0
5	36.13085889611207	37.0	35.0	37.0	35.0	37.0
6	36.067822817748265	37.0	35.0	37.0	35.0	37.0
7	36.16917269150823	37.0	36.0	37.0	35.0	37.0
8	35.97753302894354	37.0	36.0	37.0	35.0	37.0
9	37.8337983119399	39.0	38.0	39.0	35.0	39.0
10	37.392399974376474	39.0	37.0	39.0	34.0	39.0
11	37.37544426993941	39.0	37.0	39.0	34.0	39.0
12	37.41252106761739	39.0	37.0	39.0	35.0	39.0
13	37.38936049426894	39.0	37.0	39.0	34.0	39.0
14	38.7356668411717	40.0	38.0	41.0	34.0	41.0
15	38.75010658060697	40.0	38.0	41.0	34.0	41.0
16	38.80148395547692	40.0	38.0	41.0	35.0	41.0
17	38.691439754897736	40.0	38.0	41.0	34.0	41.0
18	38.14665712411284	39.0	38.0	40.0	34.0	41.0
19	37.523316302525465	37.0	37.0	40.0	34.0	41.0
20	36.72802617133744	37.0	35.0	40.0	33.0	41.0
21	36.66931516713827	37.0	35.0	40.0	33.0	41.0
22	36.67728496672255	37.0	35.0	40.0	33.0	41.0
23	36.55979006381583	37.0	35.0	40.0	33.0	41.0
24	36.48905146573185	37.0	35.0	40.0	33.0	41.0
25	36.43552480732656	36.0	35.0	40.0	33.0	41.0
26	36.32558884404772	36.0	35.0	40.0	33.0	41.0
27	36.2106076972183	36.0	35.0	40.0	33.0	41.0
28	36.05945099390558	36.0	35.0	40.0	32.0	41.0
29	35.844429865542764	36.0	35.0	40.0	31.0	41.0
30	35.55413079925515	36.0	35.0	40.0	31.0	41.0
31	34.98686794386681	36.0	35.0	40.0	27.0	41.0
32	34.02859231868595	36.0	34.0	40.0	21.0	41.0
33	32.907598479376375	35.0	33.0	40.0	15.0	41.0
34	31.92006785816054	35.0	31.0	40.0	10.0	41.0
35	31.26176638856307	35.0	28.0	40.0	7.0	41.0
36	30.876032948317793	35.0	25.0	40.0	7.0	41.0
37	30.67328681338343	35.0	24.0	40.0	7.0	41.0
38	30.49642264677751	35.0	23.0	40.0	7.0	41.0
39	30.38861608671354	35.0	23.0	40.0	7.0	41.0
40	30.21372890753221	35.0	23.0	40.0	7.0	41.0
41	30.0688190426963	35.0	22.0	40.0	7.0	41.0
42	29.982907342244136	35.0	21.0	40.0	7.0	41.0
43	29.791236754736488	35.0	21.0	40.0	7.0	41.0
44	29.60675179861677	35.0	20.0	40.0	7.0	41.0
45	29.60342074047567	35.0	20.0	40.0	7.0	41.0
46	29.42865063429267	35.0	20.0	40.0	7.0	41.0
47	29.26102639885666	35.0	20.0	39.0	7.0	41.0
48	29.229823131415547	35.0	20.0	39.0	7.0	41.0
49	29.243874100139383	35.0	20.0	39.0	7.0	41.0
50	29.106580606968272	35.0	20.0	39.0	7.0	41.0
51	28.341077822618946	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	7.0
11	7.0
12	6.0
13	7.0
14	10.0
15	19.0
16	48.0
17	97.0
18	202.0
19	451.0
20	907.0
21	1646.0
22	2742.0
23	4442.0
24	7867.0
25	15427.0
26	23889.0
27	25528.0
28	21005.0
29	16476.0
30	13776.0
31	13167.0
32	13375.0
33	15997.0
34	22000.0
35	30734.0
36	38379.0
37	44501.0
38	71803.0
39	68161.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.102940299397627	3.603860316450523	31.189130324336382	43.104069059815465
2	47.95972688857522	7.569984250368337	32.94345815965664	11.526830701399795
3	14.48612684969815	7.48604511949177	64.69674780046343	13.331080230346645
4	11.740433700235691	3.695751575515397	69.3405697699847	15.223244954264217
5	13.893030622320298	4.445681442162625	65.10694507951024	16.55434285600684
6	13.684729042276606	8.53638871769724	67.46651822694048	10.312364013085668
7	53.39809899957809	1.6617738989063615	41.99806056429185	2.942066537223691
8	53.70867378382139	4.858308538155858	38.33897713542254	3.0940405426002133
9	49.5483853866391	3.8380063131062117	39.530029224071086	7.083579076183597
10	25.821885582128917	15.564082004113017	45.51643550271808	13.097596911039982
11	18.424197442507218	16.180813723606114	49.96763925612259	15.427349577764083
12	14.267222432070048	14.496508794832883	54.323196578817736	16.913072194279327
13	16.17970926135774	15.106392848386049	56.34171178394951	12.3721861063067
14	13.913352727690414	17.852527782747856	54.658290424974986	13.575829064586745
15	12.231256723413937	15.72930955647005	56.74086444051256	15.298569279603456
16	12.876704461364808	15.524321363171486	53.79150845244959	17.807465723014122
17	12.98516265415532	16.92853466575659	53.24347428480547	16.842828395282623
18	13.73266270385612	15.171556121040227	54.134554426795134	16.961226748308516
19	14.528317307586109	17.4988789708179	52.14696416461789	15.825839556978103
20	16.159166263537948	17.766158834924862	52.38663247251546	13.688042429021733
21	14.96656792774166	20.673545257549552	51.02858569191247	13.33130112279632
22	13.929919661416054	16.90335292649362	52.27994141932235	16.88678599276798
23	14.151695680889931	18.80059817675372	50.41494646671482	16.632759675641527
24	13.650269820127278	18.205293024879115	51.106339834198124	17.03809732079548
25	13.190371739903558	21.161938463781368	49.431312388311255	16.21637740800382
26	13.457651604010524	19.039382914852588	51.39924322246742	16.103722258669475
27	15.15079223077076	18.870179298401403	50.25347408600227	15.725554384825571
28	12.889295330996292	19.32632220698064	51.24616475484252	16.53821770718055
29	14.612477330912352	18.147640095513896	50.47856349222128	16.76131908135248
30	16.37983782076345	20.571713838249295	48.58043467216247	14.468013668824787
31	16.436828072779644	21.823511350558526	45.10182037467778	16.637840201984055
32	17.706959658411918	21.958697529759736	44.041757508686594	16.292585303141756
33	18.48008323227504	20.47054509629806	42.659633451069006	18.38973822035789
34	17.95745169634357	22.596193139522295	40.49665458384967	18.949700580284464
35	19.34708609725011	23.14157659777031	38.318875922502095	19.192461382477486
36	19.214771519894676	26.315580207152937	37.26367269040377	17.205975582548614
37	20.73826674530438	25.525006129765476	37.09535264375128	16.641374481178858
38	19.17037213750997	25.235637020690994	37.06199788385033	18.531992957948702
39	21.039343154211647	24.195675367620257	37.262568228155395	17.5024132500127
40	20.03317804594121	23.444199253825303	37.8249604050284	18.697662295205085
41	19.078039093545744	24.820138322851985	36.086095041185395	20.015727542416872
42	18.108763024371065	25.142199514478396	37.202485481843745	19.546551979306795
43	18.490686069859446	24.55153310404697	38.44058766227312	18.517193163820465
44	19.32919380882642	23.993337883717796	37.20844957798498	19.469018729470807
45	19.770978708176777	23.248267650963424	36.04699707759289	20.93375656326691
46	21.03691333726522	23.894598958712994	36.21134106015123	18.857146643870564
47	17.526048742127944	24.75188255590236	39.40257428060852	18.31949442136118
48	18.01753444265522	24.213346763594274	38.278673496661206	19.490445297089302
49	18.392168037304316	22.39319297827081	39.725077257134274	19.4895617272906
50	17.949057783255913	22.25866947641863	38.52651482519676	21.265757915128702
51	17.41405627014263	21.793690869852377	36.847953100115085	23.944299759889905
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	460.0
1	1074.0
2	1688.0
3	23453.0
4	45218.0
5	29212.0
6	13206.0
7	12899.0
8	12592.0
9	12455.0
10	12318.0
11	11982.0
12	11646.0
13	11133.0
14	10620.0
15	10042.5
16	9465.0
17	9100.0
18	8735.0
19	8257.5
20	7780.0
21	7306.5
22	6833.0
23	6512.0
24	6191.0
25	5861.5
26	5395.5
27	5259.0
28	5415.5
29	5572.0
30	5809.0
31	6046.0
32	6518.0
33	6990.0
34	7651.5
35	8313.0
36	8867.0
37	9421.0
38	9821.0
39	10221.0
40	11567.0
41	12913.0
42	14198.5
43	15484.0
44	17496.0
45	19508.0
46	27874.0
47	36240.0
48	34285.5
49	32331.0
50	31633.5
51	30936.0
52	26873.5
53	22811.0
54	20014.0
55	17217.0
56	15567.5
57	13918.0
58	12957.0
59	11996.0
60	10844.0
61	9692.0
62	8794.5
63	7897.0
64	6830.0
65	5763.0
66	4788.0
67	3813.0
68	3350.0
69	2887.0
70	2377.5
71	1868.0
72	1669.5
73	1471.0
74	1127.0
75	635.5
76	488.0
77	386.5
78	285.0
79	237.0
80	189.0
81	136.0
82	83.0
83	51.5
84	20.0
85	11.0
86	2.0
87	2.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.929621163979995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75537040408155	40.320850512326736
2	7.863736483287127	7.852667670877891
3	3.2669192888462235	4.893481273961727
4	1.867159024399106	3.7290617096461545
5	1.3364838956483527	3.3365067300741216
6	0.9647499491105924	2.8901759686236517
7	0.7450249456050709	2.6039169304243193
8	0.5802240246884616	2.3176292594347734
9	0.4628651320140272	2.079961262232837
>10	2.072347267165871	16.23766040249102
>50	0.054973065411850104	1.8761929018589747
>100	0.02438321449704007	2.4672069471471336
>500	0.0035466493813876465	1.0799840925112925
>1k	0.0017733246906938233	1.9459192779804817
>5k	0.0	0.0
>10k+	4.433311726734558E-4	6.368785060408875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28772	6.355517562054211	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	2647	0.5847023142901953	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	2602	0.5747621540548122	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	2517	0.5559862958324222	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	1025	0.2264147609170571	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGTGCTT	914	0.20189569900311236	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	645	0.1424756300404896	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	593	0.13098922265738036	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	579	0.12789672836192786	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	568	0.12546691141550093	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	564	0.1245833416168002	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	513	0.11331782668336614	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	503	0.11110890218661437	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	471	0.10404034379700867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2089244967517764E-4	0.0	0.0	0.09343750621260015	0.0
2	2.2089244967517764E-4	0.0	0.0	0.4223463637789397	0.0
3	2.2089244967517764E-4	0.0	0.0	0.6226958156343259	0.0
4	2.2089244967517764E-4	0.0	0.0	1.0291379230366526	0.0
5	2.2089244967517764E-4	0.0	0.0	2.295293444574771	0.0
6	2.2089244967517764E-4	0.0	0.0	3.278706630528662	0.0
7	2.2089244967517764E-4	0.0	0.0	4.1589630424842445	0.0
8	2.2089244967517764E-4	0.0	0.0	5.32085732777568	0.0
9	2.2089244967517764E-4	0.0	0.0	5.90578053451555	0.0
10	2.2089244967517764E-4	0.0	0.0	7.476325851706063	0.0
11	2.2089244967517764E-4	0.0	0.0	9.530183848785864	0.0
12	2.2089244967517764E-4	0.0	0.0	11.832324959300566	0.0
13	2.2089244967517764E-4	0.0	0.0	12.493235168728697	0.0
14	2.2089244967517764E-4	0.0	0.0	12.759852355486638	0.0
15	2.2089244967517764E-4	0.0	0.0	13.133160595437687	0.0
16	2.2089244967517764E-4	0.0	0.0	13.88706652617907	0.0
17	2.2089244967517764E-4	0.0	0.0	14.8576679500518	0.0
18	2.2089244967517764E-4	0.0	0.0	15.751840586336918	0.0
19	2.2089244967517764E-4	0.0	0.0	16.507955441575053	0.0
20	2.2089244967517764E-4	0.0	0.0	16.986850272470836	0.0
21	2.2089244967517764E-4	0.0	0.0	17.504622174509453	0.0
22	2.2089244967517764E-4	0.0	0.0	17.99213181094257	0.0
23	2.2089244967517764E-4	0.0	0.0	18.37604288847803	0.0
24	2.2089244967517764E-4	0.0	0.0	18.650170418524926	0.0
25	2.2089244967517764E-4	0.0	0.0	18.878352319039383	0.0
26	2.2089244967517764E-4	0.0	0.0	19.068982503109062	0.0
27	2.2089244967517764E-4	0.0	0.0	19.25740376268199	0.0
28	2.2089244967517764E-4	0.0	0.0	19.440302711013036	0.0
29	2.2089244967517764E-4	0.0	0.0	19.643744657163875	0.0
30	2.2089244967517764E-4	0.0	0.0	19.846744818415363	0.0
31	2.2089244967517764E-4	0.0	0.0	20.043118206176594	0.0
32	2.2089244967517764E-4	0.0	0.0	20.244130335381005	0.0
33	2.2089244967517764E-4	0.0	0.0	20.425703929014002	0.0
34	2.2089244967517764E-4	0.0	0.0	20.612358048989528	0.0
35	2.2089244967517764E-4	0.0	0.0	20.791943610575448	0.0
36	2.2089244967517764E-4	0.0	0.0	20.97903951545032	0.0
37	2.2089244967517764E-4	0.0	0.0	21.184469493648237	0.0
38	2.2089244967517764E-4	0.0	0.0	21.365380409932207	0.0
39	2.2089244967517764E-4	0.0	0.0	21.557998626048963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACA	20	7.0287497E-4	45.000004	33
CGGCGAA	50	2.1827873E-11	45.0	31
GCGAACC	25	3.8870465E-5	45.0	33
GCGATAG	25	3.8870465E-5	45.0	9
CGCCCTT	30	2.1625765E-6	44.999996	38
CGTTTTT	14560	0.0	44.58276	1
CGATGAA	425	0.0	43.941174	19
CCGATGA	435	0.0	42.931034	18
CGGGTAT	60	3.6379788E-12	41.249996	6
ATGAATG	475	0.0	41.210526	21
GTTTGCG	55	6.002665E-11	40.909092	1
TCTTGCG	55	6.002665E-11	40.909092	1
TCGTCTG	55	6.002665E-11	40.909092	1
GGCGAAC	55	6.002665E-11	40.909092	32
GATGAAT	490	0.0	40.408165	20
GTTTTTT	16370	0.0	40.326817	2
GCTTGCG	45	1.9239451E-8	40.0	1
CCCTTCG	40	3.4520235E-7	39.375004	40
GCTACGA	40	3.4520235E-7	39.375004	10
AGCTACG	40	3.4520235E-7	39.375004	9
>>END_MODULE