##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455250
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23338165842944	33.0	31.0	34.0	30.0	34.0
2	32.45935639758375	34.0	31.0	34.0	30.0	34.0
3	32.49442504118616	34.0	31.0	34.0	31.0	34.0
4	36.058403075233386	37.0	35.0	37.0	35.0	37.0
5	36.10209774848984	37.0	35.0	37.0	35.0	37.0
6	36.06336298736958	37.0	35.0	37.0	35.0	37.0
7	36.09316419549698	37.0	36.0	37.0	35.0	37.0
8	35.92748599670511	37.0	36.0	37.0	35.0	37.0
9	37.798701812191105	39.0	38.0	39.0	35.0	39.0
10	37.39032839099396	39.0	37.0	39.0	34.0	39.0
11	37.37879846238331	39.0	37.0	39.0	34.0	39.0
12	37.38720483250961	39.0	37.0	39.0	34.0	39.0
13	37.37771993410214	39.0	37.0	39.0	34.0	39.0
14	38.74070071389347	40.0	38.0	41.0	35.0	41.0
15	38.75360131795717	40.0	38.0	41.0	34.0	41.0
16	38.86095112575508	40.0	38.0	41.0	35.0	41.0
17	38.76051180669962	40.0	38.0	41.0	34.0	41.0
18	38.25934980779791	39.0	38.0	40.0	35.0	41.0
19	37.677979132344866	38.0	37.0	40.0	35.0	41.0
20	36.836382207578254	37.0	35.0	40.0	34.0	41.0
21	36.82385941790225	37.0	35.0	40.0	33.0	41.0
22	36.80569796814937	37.0	35.0	40.0	33.0	41.0
23	36.613111477210325	37.0	35.0	40.0	33.0	41.0
24	36.547800109829765	37.0	35.0	40.0	33.0	41.0
25	36.5266051619989	37.0	35.0	40.0	33.0	41.0
26	36.4546249313564	37.0	35.0	40.0	33.0	41.0
27	36.336419549697965	37.0	35.0	40.0	33.0	41.0
28	36.236248215266336	36.0	35.0	40.0	33.0	41.0
29	35.98793410214168	36.0	35.0	40.0	32.0	41.0
30	35.62105875892367	36.0	35.0	40.0	31.0	41.0
31	35.04955299286107	36.0	35.0	40.0	27.0	41.0
32	34.18641625480505	36.0	34.0	40.0	21.0	41.0
33	33.00895112575508	36.0	33.0	40.0	15.0	41.0
34	32.0590510708402	36.0	31.0	40.0	10.0	41.0
35	31.473570565623284	36.0	29.0	40.0	7.0	41.0
36	31.0896562328391	35.0	26.0	40.0	7.0	41.0
37	30.884538165842944	35.0	24.0	40.0	7.0	41.0
38	30.688718286655682	35.0	23.0	40.0	7.0	41.0
39	30.6042811641955	35.0	23.0	40.0	7.0	41.0
40	30.43772872048325	35.0	23.0	40.0	7.0	41.0
41	30.301752883031302	35.0	23.0	40.0	7.0	41.0
42	30.15926194398682	35.0	22.0	40.0	7.0	41.0
43	30.005249862712795	35.0	21.0	40.0	7.0	41.0
44	29.829399231191655	35.0	21.0	40.0	7.0	41.0
45	29.784252608456892	35.0	20.0	40.0	7.0	41.0
46	29.611198242723777	35.0	20.0	40.0	7.0	41.0
47	29.49691817682592	35.0	20.0	40.0	7.0	41.0
48	29.43086875343218	35.0	20.0	40.0	7.0	41.0
49	29.42318725974739	35.0	20.0	39.0	7.0	41.0
50	29.24473805601318	35.0	20.0	39.0	7.0	41.0
51	28.577605711147722	35.0	20.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	1.0
10	5.0
11	4.0
12	4.0
13	9.0
14	23.0
15	31.0
16	42.0
17	98.0
18	177.0
19	453.0
20	918.0
21	1703.0
22	2821.0
23	4469.0
24	7935.0
25	14809.0
26	23367.0
27	25279.0
28	21002.0
29	16274.0
30	13692.0
31	12935.0
32	13470.0
33	15506.0
34	21989.0
35	29712.0
36	37923.0
37	42411.0
38	72026.0
39	76125.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.34859967051071	3.902471169686985	31.05194947830862	42.69697968149369
2	47.54903898956617	7.76781987918726	33.18484349258649	11.498297638660077
3	14.682262493135639	7.686545853926415	64.17287204832509	13.45831960461285
4	11.977155409115872	3.7190554640307525	68.9996705107084	15.304118616144976
5	14.160131795716637	4.374739154310818	64.81823174080176	16.646897309170786
6	13.748929159802307	8.741790225151016	67.14069192751235	10.368588687534322
7	53.61427786930258	1.7520043931905545	41.718835804503016	2.9148819330038442
8	53.9389346512905	4.969577155409116	37.916529379461835	3.17495881383855
9	49.78912685337726	3.855903349807798	39.02998352553542	7.324986271279517
10	26.0390993959363	15.620208676551345	45.13585941790225	13.204832509610103
11	18.810323997803405	16.267764964305325	49.58418451400329	15.337726523887973
12	14.423503569467325	14.575288303130149	53.90291048874245	17.098297638660075
13	16.340252608456893	15.237561779242176	56.009665019220215	12.412520593080725
14	14.001537616694124	17.92597473915431	54.32070291048874	13.751784733662822
15	12.232839099395937	15.84843492586491	56.37298187808896	15.545744096650191
16	13.02559033498078	15.452608456891817	53.51828665568369	18.00351455244371
17	13.219769357495881	17.122020867655134	52.9331136738056	16.725096101043384
18	13.868423942888523	15.360571114772103	53.67358594179022	17.09741900054915
19	14.5447556287754	17.69906644700714	51.71466227347611	16.04151565074135
20	16.31828665568369	17.72674354750137	52.158813838550245	13.796155958264688
21	15.161559582646897	20.89555189456343	50.580560131795714	13.36232839099396
22	14.14936847885777	16.9166392092257	52.032289950576605	16.901702361339925
23	14.534870950027457	19.019879187259747	49.943767160900606	16.501482701812193
24	13.909280615046676	18.330587589236682	50.79912136188908	16.961010433827568
25	13.44843492586491	21.30345963756178	49.00626029654036	16.241845140032947
26	13.5554091158704	19.045799011532125	51.09851729818781	16.300274574409666
27	15.372872048325096	18.892037342119714	49.951235584843495	15.783855024711697
28	13.106864360241627	19.617353102690828	50.9838550247117	16.291927512355848
29	14.79143327841845	18.26820428336079	50.16474464579901	16.775617792421745
30	16.439538714991762	20.856013179571665	48.21943986820428	14.48500823723229
31	16.499725425590334	21.858539264140582	44.91136738056013	16.73036792970895
32	17.90269082921472	21.984184514003292	43.801208127402525	16.31191652937946
33	18.59857221306974	20.540582097748487	42.30971993410214	18.551125755079624
34	18.1111477210324	22.81867105985722	40.15420098846788	18.915980230642504
35	19.61317957166392	23.180669961559584	38.10829214717189	19.09785831960461
36	19.170126304228447	26.440637012630425	37.17298187808896	17.21625480505217
37	20.84437122460187	25.423613399231193	37.161559582646895	16.570455793520043
38	19.087095002745745	25.18879736408567	37.056342668863266	18.667764964305327
39	21.135420098846787	24.401537616694124	37.08929159802307	17.373750686436022
40	20.01954969796815	23.525315760571115	37.68654585392641	18.768588687534322
41	19.16968698517298	24.809006040637012	36.068753432180124	19.952553542009884
42	18.208896210873146	25.02207578253707	37.1780340472268	19.590993959362986
43	18.511806699615597	24.577265238879736	38.34464579901153	18.566282262493136
44	19.532784184514004	23.925535420098846	37.09873695771554	19.44294343767161
45	19.590554640307523	23.381438769906644	36.06809445359693	20.95991213618891
46	21.06798462383306	24.085447556287754	36.10873146622735	18.73783635365184
47	17.56836902800659	24.88478857770456	39.416144975288304	18.130697419000548
48	18.060186710598572	24.371444261394835	38.14870950027458	19.419659527732016
49	18.352333882482153	22.518176825919824	39.581109280615046	19.548380010982978
50	18.09445359692477	22.40241625480505	38.3890170236134	21.114113124656782
51	17.665238879736407	21.888632619439868	36.73014827018122	23.715980230642504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	1115.0
2	1816.0
3	23406.0
4	44996.0
5	29268.0
6	13540.0
7	13013.0
8	12486.0
9	12281.0
10	12076.0
11	11838.0
12	11600.0
13	11093.0
14	10586.0
15	10022.0
16	9458.0
17	9002.0
18	8546.0
19	8064.0
20	7582.0
21	7145.5
22	6709.0
23	6436.5
24	6164.0
25	5899.0
26	5423.0
27	5212.0
28	5443.5
29	5675.0
30	5817.5
31	5960.0
32	6503.0
33	7046.0
34	7680.0
35	8314.0
36	8980.0
37	9646.0
38	10012.5
39	10379.0
40	11723.0
41	13067.0
42	14501.0
43	15935.0
44	17801.5
45	19668.0
46	27889.0
47	36110.0
48	34292.0
49	32474.0
50	31827.5
51	31181.0
52	27098.5
53	23016.0
54	20090.5
55	17165.0
56	15790.0
57	14415.0
58	13333.5
59	12252.0
60	11108.5
61	9965.0
62	9045.5
63	8126.0
64	7105.0
65	6084.0
66	4968.5
67	3853.0
68	3351.5
69	2850.0
70	2339.0
71	1828.0
72	1664.5
73	1501.0
74	1162.0
75	669.5
76	516.0
77	411.0
78	306.0
79	233.5
80	161.0
81	120.5
82	80.0
83	54.5
84	29.0
85	16.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.83954940297651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68221669545754	40.21165324934912
2	7.911282738779847	7.885895338006669
3	3.2355655419755514	4.837773860275768
4	1.9142164269475812	3.8161473671537256
5	1.3317783434839001	3.318761627194003
6	0.9528711160704094	2.849440023843632
7	0.7369771852472631	2.571142758309828
8	0.5785395283741536	2.3067319524782683
9	0.48049146018868655	2.155273008100412
>10	2.085966917558978	16.26322956471932
>50	0.0603585726335734	2.036979380369741
>100	0.02396590383962259	2.367886227248491
>500	0.0031066912384695947	0.9068971071199265
>1k	0.0022190651703354247	2.1842948616607987
>5k	0.0	0.0
>10k+	4.43813034067085E-4	6.287893674170281
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28427	6.244261394838001	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	2654	0.5829763866007688	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	2577	0.5660626029654037	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	2566	0.5636463481603514	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGTGCTT	1058	0.23239978034047226	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	1020	0.22405271828665568	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	632	0.13882482152663372	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	625	0.1372872048325096	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	614	0.13487095002745744	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	585	0.12850082372322902	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	569	0.12498627127951675	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	547	0.12015376166941241	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	528	0.11598023064250411	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.1030203185063152	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	468	0.10280065897858319	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.589785831960461E-4	0.0	0.0	0.08544755628775398	0.0
2	6.589785831960461E-4	0.0	0.0	0.40021965952773203	0.0
3	6.589785831960461E-4	0.0	0.0	0.5987918725974739	0.0
4	6.589785831960461E-4	0.0	0.0	1.0045030203185064	0.0
5	6.589785831960461E-4	0.0	0.0	2.2660076880834708	0.0
6	6.589785831960461E-4	0.0	0.0	3.2257001647446457	0.0
7	8.786381109280615E-4	0.0	0.0	4.0397583745194945	0.0
8	8.786381109280615E-4	0.0	0.0	5.172322899505766	0.0
9	8.786381109280615E-4	0.0	0.0	5.770455793520044	0.0
10	8.786381109280615E-4	0.0	0.0	7.381658429434377	0.0
11	8.786381109280615E-4	0.0	0.0	9.324766611751786	0.0
12	8.786381109280615E-4	0.0	0.0	11.581987918725975	0.0
13	8.786381109280615E-4	0.0	0.0	12.2332784184514	0.0
14	8.786381109280615E-4	0.0	0.0	12.496210873146623	0.0
15	8.786381109280615E-4	0.0	0.0	12.86853377265239	0.0
16	8.786381109280615E-4	0.0	0.0	13.59121361889072	0.0
17	8.786381109280615E-4	0.0	0.0	14.527622185612302	0.0
18	8.786381109280615E-4	0.0	0.0	15.367819879187259	0.0
19	8.786381109280615E-4	0.0	0.0	16.120153761669414	0.0
20	8.786381109280615E-4	0.0	0.0	16.592421746293244	0.0
21	8.786381109280615E-4	0.0	0.0	17.10291048874245	0.0
22	0.001098297638660077	0.0	0.0	17.574080175727623	0.0
23	0.001098297638660077	0.0	0.0	17.94552443712246	0.0
24	0.001098297638660077	0.0	0.0	18.22668863261944	0.0
25	0.001098297638660077	0.0	0.0	18.439538714991762	0.0
26	0.001098297638660077	0.0	0.0	18.623174080175726	0.0
27	0.0013179571663920922	0.0	0.0	18.81010433827567	0.0
28	0.0013179571663920922	0.0	0.0	18.99637561779242	0.0
29	0.0013179571663920922	0.0	0.0	19.176496430532673	0.0
30	0.0013179571663920922	0.0	0.0	19.378143876990663	0.0
31	0.0013179571663920922	0.0	0.0	19.565074135090608	0.0
32	0.0013179571663920922	0.0	0.0	19.771334431630972	0.0
33	0.0013179571663920922	0.0	0.0	19.958045030203184	0.0
34	0.0013179571663920922	0.0	0.0	20.137726523887974	0.0
35	0.0013179571663920922	0.0	0.0	20.324437122460186	0.0
36	0.0015376166941241077	0.0	0.0	20.506315211422294	0.0
37	0.0015376166941241077	0.0	0.0	20.694343767160902	0.0
38	0.0015376166941241077	0.0	0.0	20.88061504667765	0.0
39	0.0015376166941241077	0.0	0.0	21.085118066996156	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	20	7.0287916E-4	45.000004	3
CGGCGAA	20	7.0287916E-4	45.000004	31
GACCAAC	20	7.0287916E-4	45.000004	35
ATGCGCC	20	7.0287916E-4	45.000004	12
ATCGAAT	20	7.0287916E-4	45.000004	43
TACGAAT	75	0.0	45.0	12
CGAATGC	50	2.1827873E-11	45.0	45
GCTACGA	75	0.0	45.0	10
CGTTTTT	14550	0.0	44.365982	1
CGATGAA	430	0.0	43.430233	19
CCGATGA	425	0.0	42.88235	18
ACACGCG	100	0.0	42.75	36
CTACGAA	75	0.0	42.0	11
CGAATAT	75	0.0	42.0	14
AGCTACG	75	0.0	42.0	9
TCAAGCG	75	0.0	42.0	17
TACTGAC	70	0.0	41.785713	20
TATGGGC	60	3.6379788E-12	41.249996	4
TCGAATG	55	6.002665E-11	40.909092	44
GTTTGCG	45	1.9239451E-8	40.0	1
>>END_MODULE