##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933654.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720360
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50900660780721	34.0	31.0	34.0	31.0	34.0
2	32.71915708812261	34.0	31.0	34.0	31.0	34.0
3	32.72977400188795	34.0	31.0	34.0	31.0	34.0
4	36.22934782608696	37.0	37.0	37.0	35.0	37.0
5	36.250764895330114	37.0	37.0	37.0	35.0	37.0
6	36.24404603253929	37.0	37.0	37.0	35.0	37.0
7	36.32498750624688	37.0	37.0	37.0	35.0	37.0
8	36.24514131822978	37.0	37.0	37.0	35.0	37.0
9	38.08394552723638	39.0	39.0	39.0	37.0	39.0
10	37.40176856016436	39.0	37.0	39.0	34.0	39.0
11	37.4071464267866	39.0	37.0	39.0	34.0	39.0
12	37.51519101560331	39.0	37.0	39.0	35.0	39.0
13	37.5554333944139	39.0	37.0	39.0	35.0	39.0
14	38.96020461991227	40.0	38.0	41.0	35.0	41.0
15	38.983143150646896	40.0	38.0	41.0	35.0	41.0
16	38.99845910378144	40.0	38.0	41.0	35.0	41.0
17	38.95562496529513	40.0	38.0	41.0	35.0	41.0
18	38.35640235437837	39.0	38.0	40.0	35.0	41.0
19	37.72711977344661	38.0	37.0	40.0	35.0	41.0
20	37.02213615414515	37.0	35.0	40.0	34.0	41.0
21	36.929307568438006	37.0	35.0	40.0	33.0	41.0
22	36.92160031095563	37.0	35.0	40.0	33.0	41.0
23	36.84841051696374	37.0	35.0	40.0	33.0	41.0
24	36.73881114998056	37.0	35.0	40.0	33.0	41.0
25	36.663651507579544	37.0	35.0	40.0	33.0	41.0
26	36.601282691987336	37.0	35.0	40.0	33.0	41.0
27	36.51360430895663	37.0	35.0	40.0	33.0	41.0
28	36.34347548447998	37.0	35.0	40.0	33.0	41.0
29	36.11252568160364	37.0	35.0	40.0	32.0	41.0
30	35.75019018268643	37.0	35.0	40.0	31.0	41.0
31	35.09760397578988	37.0	35.0	40.0	25.0	41.0
32	34.06119995557777	37.0	34.0	40.0	18.0	41.0
33	32.75224609917264	37.0	33.0	40.0	12.0	41.0
34	31.629426953190073	37.0	29.0	40.0	7.0	41.0
35	30.895646621133878	37.0	23.0	40.0	7.0	41.0
36	30.502661169415294	37.0	21.0	40.0	7.0	41.0
37	30.24982369926148	36.0	21.0	40.0	7.0	41.0
38	30.09961824643234	36.0	20.0	40.0	7.0	41.0
39	29.967578710644677	36.0	18.0	40.0	7.0	41.0
40	29.839769004386696	36.0	18.0	40.0	7.0	41.0
41	29.726564495530013	36.0	18.0	40.0	7.0	41.0
42	29.618243655949804	36.0	18.0	40.0	7.0	41.0
43	29.478267810539176	36.0	18.0	40.0	7.0	41.0
44	29.31588094841468	35.0	18.0	40.0	7.0	41.0
45	29.276166083624855	35.0	18.0	40.0	7.0	41.0
46	29.107126992059527	35.0	18.0	40.0	7.0	41.0
47	28.99570492531512	35.0	17.0	40.0	7.0	41.0
48	28.906281581431507	35.0	17.0	40.0	7.0	41.0
49	28.881012271641957	35.0	17.0	40.0	7.0	41.0
50	28.789172080626354	35.0	15.0	40.0	7.0	41.0
51	28.049509967238603	35.0	15.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	4.0
13	0.0
14	11.0
15	19.0
16	50.0
17	130.0
18	309.0
19	692.0
20	1257.0
21	2142.0
22	3726.0
23	6797.0
24	13946.0
25	30286.0
26	46835.0
27	46300.0
28	34151.0
29	24612.0
30	19122.0
31	17047.0
32	17869.0
33	20325.0
34	28524.0
35	37342.0
36	47149.0
37	63717.0
38	117279.0
39	140661.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.82811372091732	3.520600810705758	35.58234771503137	44.06893775334555
2	48.923871397634514	3.4280082181131655	35.35676606141374	12.29135432283858
3	14.500388694541618	3.415098006552279	68.44383363873618	13.640679660169916
4	11.187739463601533	3.650258204231218	69.29021600310956	15.871786329057693
5	10.84416125270698	4.366566716641679	69.4727636181909	15.316508412460436
6	13.622216669443057	5.002082292187239	71.30809595202399	10.067605086345715
7	56.594341718029874	1.2622855239047144	39.66016991504248	2.483202843022933
8	58.06180243211727	1.409295352323838	38.18077072574824	2.3481314898106502
9	53.984951968460216	3.5294852573713142	39.48414681548115	3.001415958687323
10	23.051668610139377	16.53645399522461	48.269754011882945	12.142123382753066
11	17.0338441890166	19.470403687045366	49.54800377588983	13.947748348048197
12	13.636653895274584	16.93444944194569	55.039563551557556	14.389333111222166
13	14.884641012826918	17.486950968959963	55.80515297906602	11.823255039147092
14	14.832861347104226	17.980593036814927	54.86728857793326	12.319257038147594
15	13.201732467099783	18.694958076517295	55.55277916597257	12.55053029041035
16	13.171469820645232	17.862041201621413	54.58409684046865	14.382392137264702
17	13.500333166749959	18.008912210561387	53.6448442445444	14.84591037814426
18	14.297018157587873	16.926536731634183	54.89338664001333	13.883058470764617
19	14.718890554722638	19.480537509023264	53.0275140207674	12.773057915486701
20	15.18449108778944	19.100033316674995	52.6418735076906	13.073602087844966
21	14.897828863346104	20.047615081348212	52.48500749625188	12.569548559053805
22	14.091842967405185	18.993697595646623	52.24943084013549	14.665028596812704
23	12.90882336609473	20.23668721194958	51.432478205341766	15.422011216613916
24	12.959353656505082	19.431534232883557	52.563162863012934	15.045949247598422
25	12.936864900882892	21.156782719751234	51.10639124882004	14.799961130545839
26	12.980593036814925	20.873868621244934	52.211810761286024	13.933727580654118
27	13.235326781053919	20.39272030651341	52.091315453384425	14.280637459048254
28	13.041257149203176	19.415292353823087	52.23749236492865	15.305958132045088
29	13.391221056138598	19.086290188239214	51.55269587428508	15.969792881337108
30	14.53648175912044	19.753734243989115	50.457965461713584	15.251818535176856
31	13.969404186795492	21.72469320895108	48.0266811038925	16.27922150036093
32	13.59736798267533	21.887528457993223	47.003859181520355	17.511244377811096
33	14.963906935421178	23.00599700149925	43.28335832083958	18.74673774223999
34	15.147426286856572	23.990227108667888	40.81264923094008	20.049697373535455
35	15.920511966239104	25.083152868010437	38.798933866400134	20.197401299350325
36	16.695679937808876	26.13179521350436	36.77952690321506	20.39299794547171
37	16.477733355544448	27.811788550169357	35.4045199622411	20.305958132045088
38	17.18224221222722	28.479926703315012	34.868260314287305	19.46957077017047
39	17.8135932033983	28.74104614359487	34.44944194569382	18.995918707313013
40	18.382614248431338	28.345549447498474	34.99458604031317	18.27725026375701
41	18.648731189960575	27.27913820867344	34.70792381586985	19.36420678549614
42	18.558637347992672	26.39111000055528	34.63226719973347	20.417985451718586
43	17.51596424010217	26.025320672996834	35.93425509467488	20.52445999222611
44	17.3178688433561	26.875867621744682	35.051502026764396	20.754761508134823
45	18.281553667610638	27.033011272141707	34.349075462268864	20.336359597978788
46	18.794908101504802	26.989421955688826	34.52051751901827	19.695152423788105
47	17.606752179465822	27.52790271530901	35.28513521017269	19.580209895052477
48	17.38269754011883	26.382503192848024	35.84596590593592	20.38883336109723
49	18.64928646787717	25.63121217169193	35.47170859014937	20.247792770281524
50	17.6925426175801	25.36204120162141	35.10883447165306	21.836581709145428
51	17.367427397412406	25.00735743239491	34.34463323893609	23.280581931256595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	897.0
2	1531.0
3	26301.5
4	51072.0
5	35324.0
6	19576.0
7	19427.5
8	19279.0
9	19615.5
10	19952.0
11	20043.0
12	20134.0
13	19748.0
14	19362.0
15	18682.5
16	18003.0
17	17369.0
18	16735.0
19	16140.5
20	15546.0
21	14756.0
22	13966.0
23	13371.0
24	12776.0
25	12354.5
26	11914.5
27	11896.0
28	12139.5
29	12383.0
30	12834.5
31	13286.0
32	14518.5
33	15751.0
34	17189.5
35	18628.0
36	20725.5
37	22823.0
38	23601.0
39	24379.0
40	26141.0
41	27903.0
42	30726.0
43	33549.0
44	36311.5
45	39074.0
46	43153.0
47	47232.0
48	50107.0
49	52982.0
50	50363.0
51	47744.0
52	41588.5
53	35433.0
54	30013.5
55	24594.0
56	20934.5
57	17275.0
58	14989.5
59	12704.0
60	10771.0
61	8838.0
62	7247.5
63	5657.0
64	4623.5
65	3590.0
66	2754.0
67	1918.0
68	1429.0
69	940.0
70	761.5
71	583.0
72	509.5
73	436.0
74	364.5
75	212.5
76	132.0
77	118.0
78	104.0
79	77.5
80	51.0
81	46.0
82	41.0
83	25.0
84	9.0
85	5.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.964974262410834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5544394102511	41.054549312663795
2	7.731255256998394	7.880464503781033
3	3.2820537250194994	5.018093508704052
4	1.9135825189466091	3.9010273530845287
5	1.3580974135300852	3.4607699863203756
6	0.9612023781684538	2.9392592678593994
7	0.7484807192507174	2.670241041476649
8	0.5785312755759444	2.358786525578217
9	0.49371295082413946	2.264586104859405
>10	2.287689176789883	18.543442896721597
>50	0.06345079857575854	2.2258866008174194
>100	0.024508849962911024	2.2679363558174086
>500	0.002178564441122768	0.697271418612783
>1k	5.44641110280692E-4	0.39887700180993996
>5k	0.0	0.0
>10k+	2.72320555140346E-4	4.3188081218934276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31118	4.31978455216836	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	1805	0.25056915986451217	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	1069	0.1483980232106169	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	795	0.11036148592370482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7763895829862847E-4	0.0	0.0	0.10578044311177745	0.0
2	2.7763895829862847E-4	0.0	0.0	0.34121827974901436	0.0
3	2.7763895829862847E-4	0.0	0.0	0.5512521517019268	0.0
4	2.7763895829862847E-4	0.0	0.0	0.840274307290799	0.0
5	2.7763895829862847E-4	0.0	0.0	1.4624632128380255	0.0
6	2.7763895829862847E-4	0.0	0.0	2.285801543672608	0.0
7	2.7763895829862847E-4	0.0	0.0	2.974762618690655	0.0
8	2.7763895829862847E-4	0.0	0.0	3.972180576378477	0.0
9	2.7763895829862847E-4	0.0	0.0	4.527319673496585	0.0
10	2.7763895829862847E-4	0.0	0.0	5.46351824087956	0.0
11	2.7763895829862847E-4	0.0	0.0	7.310650230440335	0.0
12	2.7763895829862847E-4	0.0	0.0	8.83391637514576	0.0
13	2.7763895829862847E-4	0.0	0.0	9.433061247154201	0.0
14	2.7763895829862847E-4	0.0	0.0	9.668637903270588	0.0
15	2.7763895829862847E-4	0.0	0.0	9.92698095396746	0.0
16	2.7763895829862847E-4	0.0	0.0	10.535982008995502	0.0
17	2.7763895829862847E-4	0.0	0.0	11.380698539619079	0.0
18	2.7763895829862847E-4	0.0	0.0	12.199594647120884	0.0
19	2.7763895829862847E-4	0.0	0.0	12.713920817369093	0.0
20	2.7763895829862847E-4	0.0	0.0	13.159531345438392	0.0
21	2.7763895829862847E-4	0.0	0.0	13.69634627130879	0.0
22	2.7763895829862847E-4	0.0	0.0	14.198039868954412	0.0
23	2.7763895829862847E-4	0.0	0.0	14.620467544005775	0.0
24	2.7763895829862847E-4	0.0	0.0	14.906435671053362	0.0
25	2.7763895829862847E-4	0.0	0.0	15.146593369981677	0.0
26	2.7763895829862847E-4	0.0	0.0	15.345660503081792	0.0
27	2.7763895829862847E-4	0.0	0.0	15.548198123160642	0.0
28	2.7763895829862847E-4	0.0	0.0	15.733244488866678	0.0
29	4.164584374479427E-4	0.0	0.0	15.92120606363485	0.0
30	6.940973957465711E-4	0.0	0.0	16.126658892775833	0.0
31	6.940973957465711E-4	0.0	0.0	16.312676994835915	0.0
32	8.329168748958854E-4	1.3881947914931423E-4	0.0	16.50785718251985	0.0
33	8.329168748958854E-4	1.3881947914931423E-4	0.0	16.697901049475263	0.0
34	8.329168748958854E-4	1.3881947914931423E-4	0.0	16.88822255538897	0.0
35	8.329168748958854E-4	1.3881947914931423E-4	0.0	17.0650785718252	0.0
36	8.329168748958854E-4	1.3881947914931423E-4	0.0	17.262202232217223	0.0
37	8.329168748958854E-4	1.3881947914931423E-4	0.0	17.46362929646288	0.0
38	8.329168748958854E-4	1.3881947914931423E-4	0.0	17.673663168415793	0.0
39	8.329168748958854E-4	1.3881947914931423E-4	0.0	17.88022655338997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.031531E-4	45.000004	1
CGTTGAT	20	7.031531E-4	45.000004	25
TATTGCG	40	6.8084773E-9	45.000004	1
AGTGCGG	40	6.8084773E-9	45.000004	2
TCGGACC	20	7.031531E-4	45.000004	5
TAGTGCG	20	7.031531E-4	45.000004	1
ATCTCCG	25	3.8893508E-5	45.0	1
TTCACGG	25	3.8893508E-5	45.0	2
TGCGGAT	25	3.8893508E-5	45.0	4
ACGGGTA	25	3.8893508E-5	45.0	5
CTGCGGC	25	3.8893508E-5	45.0	3
CTGCGCG	25	3.8893508E-5	45.0	1
TGCGTAG	25	3.8893508E-5	45.0	1
CGTTTTT	24615	0.0	44.424133	1
TCAAGCG	50	1.0804797E-9	40.5	17
GCGTAGG	50	1.0804797E-9	40.5	2
GTTTTTT	27600	0.0	40.116848	2
TTGCGGA	45	1.9266736E-8	40.0	3
CGGGTGG	80	0.0	39.375004	6
TGGCGCG	40	3.4558252E-7	39.375004	1
>>END_MODULE