FastQCFastQC Report
Sat 14 Jan 2017
SRR2933653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725882
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310964.283891872232677No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC17970.24756089832782738No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC10450.1439627928506286No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT7950.1095219333169854RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7770.1070421914305631No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC7330.1009806001526419RNA PCR Primer, Index 13 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA551.8189894E-1245.06
ATCTCCG207.031567E-445.01
GGGTCGA207.031567E-445.07
CATTGCG253.8893802E-545.01
TATTGCG351.2111013E-745.01
CTACGAA750.045.011
TACGAAT750.045.012
GTTTCCG302.1643918E-644.9999961
CGTTTTT240900.044.48631
GGCGATA556.002665E-1140.9090928
TGCGTTG501.0804797E-940.51
TGCTTCG451.9266736E-840.01
GTTTTTT273700.039.8702962
CGAATAT850.039.70588314
AGCTACG850.039.7058839
GACACGA403.4558798E-739.37525
TTGCGGA403.4558798E-739.3753
TTGGGTA9500.038.8421065
TGGGCGA8550.038.684216
GTTCTCG356.245109E-638.5714261
Produced by FastQC (version 0.11.5)