##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933650.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93272
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.009413328758896	33.0	31.0	34.0	30.0	34.0
2	32.20928038425251	34.0	31.0	34.0	30.0	34.0
3	32.31598979329274	34.0	31.0	34.0	30.0	34.0
4	35.88687923492581	37.0	35.0	37.0	35.0	37.0
5	35.911366755296335	37.0	35.0	37.0	35.0	37.0
6	35.86265974783429	37.0	35.0	37.0	35.0	37.0
7	35.91092718071876	37.0	35.0	37.0	35.0	37.0
8	35.71271121022386	37.0	35.0	37.0	35.0	37.0
9	37.545758641392915	39.0	37.0	39.0	35.0	39.0
10	37.06806973153787	39.0	37.0	39.0	33.0	39.0
11	37.068219830174115	39.0	37.0	39.0	33.0	39.0
12	37.05814177888327	39.0	37.0	39.0	33.0	39.0
13	37.00712968522172	39.0	37.0	39.0	33.0	39.0
14	37.93708722875032	40.0	37.0	41.0	33.0	41.0
15	38.00492109100266	40.0	37.0	41.0	33.0	41.0
16	38.121483403379365	40.0	37.0	41.0	33.0	41.0
17	38.08706793035423	40.0	37.0	41.0	33.0	41.0
18	37.84345784372588	39.0	37.0	40.0	33.0	41.0
19	37.50473882837293	39.0	37.0	40.0	33.0	41.0
20	37.084644909511965	38.0	35.0	40.0	33.0	41.0
21	36.928842525087916	38.0	35.0	40.0	33.0	41.0
22	36.988785487606144	38.0	35.0	40.0	33.0	41.0
23	36.97930783086028	38.0	35.0	40.0	33.0	41.0
24	36.89579938245132	38.0	35.0	40.0	33.0	41.0
25	36.793989621751436	38.0	35.0	40.0	33.0	41.0
26	36.665741058409814	38.0	35.0	40.0	32.0	41.0
27	36.569742259198904	38.0	35.0	40.0	32.0	41.0
28	36.46810403979758	38.0	35.0	40.0	32.0	41.0
29	36.36210223861394	38.0	35.0	40.0	31.0	41.0
30	36.212078651685395	38.0	35.0	40.0	31.0	41.0
31	35.81028604511536	38.0	35.0	40.0	30.0	41.0
32	35.34193541470109	38.0	35.0	40.0	27.0	41.0
33	34.93390299339566	38.0	35.0	40.0	24.0	41.0
34	34.52851874088687	38.0	34.0	40.0	21.0	41.0
35	34.12903122051634	38.0	34.0	40.0	18.0	41.0
36	33.89936958572776	38.0	33.0	40.0	18.0	41.0
37	33.719572862166565	38.0	33.0	40.0	17.0	41.0
38	33.54921091002659	37.0	33.0	40.0	15.0	41.0
39	33.521775023586926	37.0	33.0	40.0	15.0	41.0
40	33.276578179946824	37.0	33.0	40.0	15.0	41.0
41	33.05758426966292	37.0	32.0	40.0	15.0	41.0
42	32.90491251393773	37.0	32.0	40.0	14.0	41.0
43	32.833058152500215	37.0	32.0	40.0	12.0	41.0
44	32.649391028390085	36.0	31.0	40.0	11.0	41.0
45	32.58562055064757	36.0	31.0	40.0	10.0	41.0
46	32.175883437687624	35.0	31.0	40.0	10.0	41.0
47	31.8803713869114	35.0	30.0	39.0	10.0	41.0
48	31.88289089973411	35.0	30.0	39.0	10.0	41.0
49	31.979908225405268	35.0	31.0	39.0	10.0	41.0
50	31.88087528947594	35.0	30.0	39.0	10.0	41.0
51	30.65171755725191	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	3.0
12	2.0
13	4.0
14	3.0
15	7.0
16	16.0
17	19.0
18	40.0
19	77.0
20	143.0
21	215.0
22	324.0
23	534.0
24	949.0
25	1665.0
26	2324.0
27	2756.0
28	2721.0
29	2649.0
30	2661.0
31	2872.0
32	3251.0
33	4093.0
34	6110.0
35	7729.0
36	9747.0
37	12088.0
38	18072.0
39	12193.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.58538468136204	5.405695171112445	39.02993395660005	29.978986190925465
2	37.25555364954113	4.574792006175487	38.5903593790205	19.579294965262886
3	19.91272836435372	5.462518226262972	55.54721674243074	19.077536666952568
4	14.931597907196156	5.629770992366412	56.400634702804695	23.037996397632728
5	13.212968522171714	6.462818423535466	56.732995968779484	23.59121708551334
6	17.58941590187838	7.473839951968436	61.22630585813534	13.71043828801784
7	64.7461188781199	2.877605283471996	27.664250793378503	4.71202504502959
8	63.41238528175658	2.9966120593532892	27.309374731966724	6.281627926923407
9	58.252208594219056	5.3413671841495844	29.809589158589933	6.596835063041427
10	29.426837636160908	19.793721588472422	35.71382622866455	15.065614546702118
11	21.300068616519425	17.477914057809418	39.617462904194184	21.60455442147697
12	17.922849300969208	15.239300111501844	45.79938245132516	21.038468136203793
13	21.895102495925894	16.02946221802899	47.85680590102067	14.218629385024444
14	19.042156274122995	17.441461531863798	47.29393601509563	16.222446178917576
15	14.562784115275754	16.42400720473454	48.51187923492581	20.5013294450639
16	15.69602881893816	16.47439746118878	45.52920490608114	22.30036881379192
17	14.758984475512479	17.275280898876407	44.10648426108585	23.85925036452526
18	16.91182777253624	15.044171884381166	46.30006861651943	21.74393172656317
19	15.953340766789603	18.485719186894244	47.147053778197105	18.41388626811905
20	16.22459044514967	22.48477570975212	44.050733339051376	17.239900506046833
21	15.434428338622524	22.924350287331674	45.25152242902479	16.389698945021014
22	13.223689853332191	20.237584698516166	43.781627926923406	22.757097521228236
23	13.442405009005917	21.974440346513425	41.51728278583069	23.06587185864997
24	14.544557852302942	22.295008148211682	40.836478257140406	22.32395574234497
25	15.02808988764045	23.401449523972897	38.06501415215713	23.505446436229523
26	13.13041427223604	24.927094948108756	39.68929582296938	22.253194956685824
27	15.701389484518396	22.41723132344112	42.172356119735824	19.709023072304657
28	12.267347113817651	22.511579037653316	41.73707007462046	23.48400377390857
29	14.826528861823485	21.273265288618234	40.074191611630496	23.82601423792778
30	22.20816536581182	20.850844840895448	38.52924779140578	18.411742001886957
31	20.919461360322497	27.166781027532377	33.08924436057981	18.824513251565314
32	17.445750064327985	26.632858735740626	33.021699974268806	22.89969122566258
33	20.95484175315207	21.188566772450468	30.980358521314006	26.876232953083456
34	19.565357234754266	23.160219572862168	32.75152242902479	24.52290076335878
35	18.808431254824598	27.26756154044086	30.417488635388967	23.50651856934557
36	23.65447293936015	28.798567630156963	27.294364868342054	20.252594562140835
37	23.06479972553392	28.090959773565487	29.580152671755727	19.264087829144867
38	18.35063041427224	27.558109614889787	33.071018097606995	21.02024187323098
39	21.044900934900078	20.0606827343683	31.592546530577238	27.30186980015439
40	25.2787546101724	21.56274122995111	29.668067587271636	23.490436572604857
41	22.2864310832833	26.91054121279698	26.59104554421477	24.21198215970495
42	20.762929925379538	27.04991851788318	28.711724847757097	23.475426708980187
43	19.8987906338451	21.079209194613604	33.996268976756156	25.025731194785145
44	20.25152242902479	20.963418818080452	33.003473711295996	25.781585041598763
45	23.819581439231495	17.65803242130543	30.97070932326958	27.5516768161935
46	25.52212882751522	19.437773393944592	33.318680847414015	21.72141693112617
47	18.70121794321983	24.817737370271892	36.56831632215456	19.91272836435372
48	17.50471738571061	23.71558452697487	36.75915601681105	22.020542070503474
49	22.299296680675873	18.33347628441547	37.16335020156102	22.20387683334763
50	21.937987820567802	16.257826571747145	34.3211253109186	27.48306029676645
51	20.05103353632387	16.253538039282958	31.214083540612403	32.48134488378077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	544.0
2	817.0
3	3644.5
4	6472.0
5	3997.5
6	1523.0
7	1389.5
8	1256.0
9	1230.0
10	1204.0
11	1187.0
12	1170.0
13	1112.5
14	1055.0
15	969.5
16	884.0
17	820.0
18	756.0
19	692.5
20	629.0
21	577.5
22	526.0
23	536.0
24	546.0
25	560.5
26	611.5
27	648.0
28	638.0
29	628.0
30	663.5
31	699.0
32	761.5
33	824.0
34	897.5
35	971.0
36	1031.5
37	1092.0
38	1246.0
39	1400.0
40	1755.5
41	2111.0
42	2497.0
43	2883.0
44	3520.5
45	4158.0
46	5711.0
47	7264.0
48	9461.5
49	11659.0
50	12095.5
51	12532.0
52	10921.5
53	9311.0
54	7319.0
55	5327.0
56	4272.5
57	3218.0
58	2810.0
59	2402.0
60	2180.5
61	1959.0
62	1815.0
63	1671.0
64	1594.5
65	1518.0
66	1270.5
67	1023.0
68	870.5
69	718.0
70	607.5
71	497.0
72	440.5
73	384.0
74	363.0
75	270.0
76	198.0
77	135.0
78	72.0
79	57.0
80	42.0
81	32.0
82	22.0
83	14.5
84	7.0
85	5.0
86	3.0
87	1.5
88	0.0
89	1.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	93272.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.48897847156704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.538638582787	48.9074963547474
2	11.650857860231552	14.327986962861308
3	4.231761751987725	7.806201217943219
4	1.727925791602734	4.249935672013037
5	0.8264751011298647	2.5409554850330216
6	0.4638024829125401	1.7111244532121108
7	0.34349281629236994	1.4784715670297623
8	0.21969591295857163	1.08071018097607
9	0.13600223183149673	0.7526374474654773
>10	0.80729529920491	8.827944077536667
>50	0.03661598549309527	1.6403636675529636
>100	0.015692565211326545	2.270777939789004
>500	0.0	0.0
>1k	0.0017436183568140605	4.405394973839952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4109	4.405394973839952	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCC	386	0.41384338279440774	No Hit
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	311	0.33343339909083114	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	304	0.3259284672784973	TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	265	0.28411527575263745	TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	222	0.23801355176258687	TruSeq Adapter, Index 21 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.20263315893301312	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	182	0.19512822712067932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144	0.15438716871086713	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	115	0.12329530834548418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3795351230808817	0.0
2	0.0	0.0	0.0	1.3980615833261858	0.0
3	0.0	0.0	0.0	2.0156102581696542	0.0
4	0.0	0.0	0.0	2.921562741229951	0.0
5	0.0	0.0	0.0	4.923235268890985	0.0
6	0.0	0.0	0.0	6.607556394201904	0.0
7	0.0	0.0	0.0	8.000257311947852	0.0
8	0.0	0.006432798696286131	0.0	10.238871258255426	0.0
9	0.0	0.006432798696286131	0.0	11.248820653572347	0.0
10	0.0	0.006432798696286131	0.0	13.639677502358692	0.0
11	0.0	0.006432798696286131	0.0	19.516039111416074	0.0
12	0.0	0.006432798696286131	0.0	24.771635646281844	0.0
13	0.0	0.006432798696286131	0.0	26.564242216313577	0.0
14	0.0	0.006432798696286131	0.0	27.187151556737284	0.0
15	0.0	0.006432798696286131	0.0	28.065228578780342	0.0
16	0.0	0.006432798696286131	0.0	30.048674843468564	0.0
17	0.0	0.006432798696286131	0.0	32.52744660777082	0.0
18	0.0	0.006432798696286131	0.0	34.841109872201734	0.0
19	0.0	0.006432798696286131	0.0	36.08907281928124	0.0
20	0.0	0.006432798696286131	0.0	37.259842182005315	0.0
21	0.0	0.006432798696286131	0.0	38.588215112788404	0.0
22	0.0	0.006432798696286131	0.0	39.70430568659405	0.0
23	0.0	0.006432798696286131	0.0	40.45372673471138	0.0
24	0.0	0.006432798696286131	0.0	40.902950510335366	0.0
25	0.0	0.006432798696286131	0.0	41.19778711724848	0.0
26	0.0	0.006432798696286131	0.0	41.42186293850244	0.0
27	0.0	0.006432798696286131	0.0	41.64272236040827	0.0
28	0.0	0.006432798696286131	0.0	41.817480058324044	0.0
29	0.0	0.006432798696286131	0.0	42.03726734711382	0.0
30	0.0	0.006432798696286131	0.0	42.25383823655545	0.0
31	0.0	0.006432798696286131	0.0	42.408225405266315	0.0
32	0.0	0.006432798696286131	0.0	42.57011750578952	0.0
33	0.0	0.006432798696286131	0.0	42.728793206964575	0.0
34	0.0	0.006432798696286131	0.0	42.864954112702634	0.0
35	0.0	0.006432798696286131	0.0	43.046144609314695	0.0
36	0.0	0.006432798696286131	0.0	43.21446950853418	0.0
37	0.0	0.006432798696286131	0.0	43.34848614804014	0.0
38	0.0010721331160476885	0.006432798696286131	0.0	43.51466678102753	0.0
39	0.0010721331160476885	0.006432798696286131	0.0	43.93708722875032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	30	2.1440374E-6	45.000004	17
CGCGGGA	35	1.1959492E-7	45.000004	4
GCACCGA	30	2.1440374E-6	45.000004	9
ATTCCTC	30	2.1440374E-6	45.000004	11
TGTATGG	35	1.1959492E-7	45.000004	2
GGGTGTC	30	2.1440374E-6	45.000004	7
TGCGGGA	30	2.1440374E-6	45.000004	4
CATGAAC	20	6.9999555E-4	45.0	20
GGCACGT	20	6.9999555E-4	45.0	11
GGCATGA	20	6.9999555E-4	45.0	18
CGGGGCT	20	6.9999555E-4	45.0	6
CACGTGG	20	6.9999555E-4	45.0	13
ACCACAG	20	6.9999555E-4	45.0	35
GGACTAA	20	6.9999555E-4	45.0	8
ACACTTG	20	6.9999555E-4	45.0	25
TGTTAGG	25	3.8632035E-5	45.0	2
GCACGTG	20	6.9999555E-4	45.0	12
CGGGAGG	45	3.765308E-10	45.0	6
TCGTTCA	25	3.8632035E-5	45.0	16
AGGCACG	20	6.9999555E-4	45.0	10
>>END_MODULE