##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933648.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143633
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953422959904756	33.0	31.0	34.0	30.0	34.0
2	32.197628678646275	34.0	31.0	34.0	30.0	34.0
3	32.22693252943265	33.0	31.0	34.0	30.0	34.0
4	35.890742378144296	37.0	35.0	37.0	35.0	37.0
5	35.93379655093189	37.0	35.0	37.0	35.0	37.0
6	35.92717550980624	37.0	35.0	37.0	35.0	37.0
7	35.97822923701378	37.0	35.0	37.0	35.0	37.0
8	35.79556926333085	37.0	35.0	37.0	35.0	37.0
9	37.5955525540788	39.0	37.0	39.0	35.0	39.0
10	37.137656388155925	39.0	37.0	39.0	33.0	39.0
11	37.19428682823585	39.0	37.0	39.0	34.0	39.0
12	37.263045400430265	39.0	37.0	39.0	34.0	39.0
13	37.18588346689131	39.0	37.0	39.0	33.0	39.0
14	38.25804654919134	40.0	38.0	41.0	33.0	41.0
15	38.28246294375248	40.0	38.0	41.0	33.0	41.0
16	38.38649196215354	40.0	38.0	41.0	34.0	41.0
17	38.397234618785376	40.0	38.0	41.0	34.0	41.0
18	37.8979273565267	39.0	38.0	40.0	34.0	41.0
19	37.35326839932328	37.0	37.0	40.0	34.0	41.0
20	36.621556327584884	37.0	35.0	40.0	33.0	41.0
21	36.49954397666274	37.0	35.0	40.0	33.0	41.0
22	36.51212465102031	37.0	35.0	40.0	33.0	41.0
23	36.4528276928004	37.0	35.0	40.0	33.0	41.0
24	36.28134899361567	37.0	35.0	40.0	33.0	41.0
25	36.165734893791814	36.0	35.0	40.0	32.0	41.0
26	36.153390933838324	36.0	35.0	40.0	32.0	41.0
27	36.080837968990416	36.0	35.0	40.0	32.0	41.0
28	35.92864453154915	36.0	35.0	40.0	32.0	41.0
29	35.82776938447293	36.0	35.0	40.0	31.0	41.0
30	35.616453043520636	36.0	35.0	40.0	31.0	41.0
31	35.0449270014551	36.0	35.0	40.0	27.0	41.0
32	34.37075741647114	36.0	34.0	40.0	23.0	41.0
33	33.637924432407594	36.0	33.0	40.0	18.0	41.0
34	33.0358204590867	36.0	33.0	40.0	15.0	41.0
35	32.65846985024333	37.0	33.0	40.0	10.0	41.0
36	32.34290866305097	36.0	31.0	40.0	10.0	41.0
37	31.940891020865678	35.0	31.0	40.0	9.0	41.0
38	31.785063321103088	35.0	30.0	40.0	9.0	41.0
39	31.81293296108833	36.0	30.0	40.0	9.0	41.0
40	31.695919461405108	35.0	30.0	40.0	8.0	41.0
41	31.466738145133778	35.0	29.0	40.0	8.0	41.0
42	31.10982155911246	35.0	28.0	40.0	8.0	41.0
43	30.798848454046077	35.0	26.0	39.0	7.0	41.0
44	30.69071870670389	35.0	26.0	39.0	7.0	41.0
45	30.68455020782132	35.0	25.0	39.0	7.0	41.0
46	30.230706035521084	35.0	23.0	39.0	7.0	41.0
47	29.8362911030195	35.0	23.0	39.0	7.0	40.0
48	29.756936080148712	35.0	23.0	39.0	7.0	40.0
49	29.769857901735673	35.0	23.0	39.0	7.0	40.0
50	29.663858584030134	35.0	23.0	39.0	7.0	40.0
51	28.424421964311822	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	6.0
15	9.0
16	6.0
17	28.0
18	76.0
19	144.0
20	256.0
21	402.0
22	679.0
23	1127.0
24	2052.0
25	4012.0
26	5831.0
27	6078.0
28	5359.0
29	4782.0
30	4577.0
31	4789.0
32	5197.0
33	6076.0
34	8420.0
35	12540.0
36	16803.0
37	16636.0
38	24546.0
39	13198.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.44353317134642	4.037373027089875	34.27067595886739	43.24841784269632
2	48.91981647671496	3.9106611990280786	32.27113546329882	14.898386860958137
3	14.307297069614922	3.917623387383122	64.78942861320171	16.985650929800254
4	12.215855687759776	4.2643403674643015	65.48843232404809	18.031371620727825
5	10.234416881914324	5.126955504654223	66.35661721192206	18.282010401509403
6	13.824817416610388	6.236032109612694	69.01826182005529	10.920888653721637
7	50.400673939832764	2.4639184588499856	42.33010519866605	4.8053024026512015
8	48.21245813984252	2.5558193451365634	42.07807398021346	7.153648534807461
9	43.50671503066844	4.473902236951118	45.19365326909554	6.825729463284899
10	21.606455341042796	16.125124449116846	49.23938092221147	13.029039287628889
11	14.318436570982993	14.140900767929377	52.311794643292274	19.228868017795353
12	13.770512347441047	11.510586007393844	57.41438248870385	17.30451915646126
13	16.77608906031344	12.744982002743102	58.45244477244087	12.026484164502586
14	13.697409369713087	15.058517193124143	56.325496229975	14.918577207187763
15	9.44629716012337	13.835260699142953	59.11663754151205	17.601804599221627
16	8.484122729456322	14.039949036781241	56.64436445663601	20.831563777126426
17	8.236268824016765	14.104001169647642	54.69355927955275	22.966170726782842
18	9.041794016695327	12.991443470511651	59.292084688059155	18.67467782473387
19	9.6419346529001	15.107252511609449	59.619307540746206	15.631505294744244
20	9.697632159740449	19.26646383491259	56.41530845975507	14.620595545591891
21	8.965209944789846	19.968252421101003	56.987600342539665	14.078937291569485
22	7.40985706627307	19.04993977707073	53.403465777363145	20.13673737929306
23	7.622203811101906	19.079180968161914	51.5501312372505	21.748483983485688
24	9.536109389903434	18.469989487095585	51.28835295510085	20.705548167900133
25	7.731510168276093	20.465352669651125	48.78683867913362	23.016298482939156
26	8.00164307645179	20.56143086895073	51.14005834313842	20.296867711459065
27	11.039942074592886	19.88679481734699	52.54642039085725	16.52684271720287
28	7.712016040881969	17.62129872661575	52.437114033683066	22.22957119881921
29	9.989347851816783	15.12953151434559	52.558952329896336	22.322168303941297
30	17.557246593749348	16.869382384271024	48.506262488425364	17.06710853355427
31	15.77910368787117	23.816253924933687	42.88568782939854	17.518954557796608
32	13.749625782375915	21.889120188257575	43.09733835539187	21.263915673974644
33	18.026498088879297	18.591131564473347	41.91515877270543	21.46721157394192
34	17.095653505809945	17.6136403194252	40.95925031155792	24.331455863206923
35	18.03346027723434	16.45513217714592	38.78565510711327	26.725752438506472
36	21.969185354340574	20.9868205774439	37.69816128605543	19.345832782160088
37	20.823209151100375	24.811846859704943	39.12053636699087	15.244407622203813
38	17.291987217422182	23.656123592767678	43.28044390912952	15.771445280680624
39	18.197767922413373	18.343277659033788	47.33034887525847	16.12860554329437
40	24.47278828681431	14.973578495192609	43.45867593101864	17.09495728697444
41	27.028607631950873	18.591131564473347	35.318485306301476	19.061775497274304
42	23.397826404795556	23.41592809451867	36.32591396127631	16.86033153940947
43	18.213084736794467	21.733167169104593	43.426649864585436	16.627098229515504
44	16.197531208009302	17.924153920060153	43.31107753789171	22.567237334038833
45	21.946906351604436	13.778170754631597	41.69933093369908	22.575591960064887
46	24.72690816177341	15.1629500184498	44.16464183022008	15.945499989556716
47	17.581614252992	21.270181643494183	45.53340806082168	15.61479604269214
48	16.2448740888236	19.539381618430305	47.05951974824727	17.156224544498826
49	20.51756908231395	14.656102706202615	47.42364219921606	17.402686012267377
50	19.801856119415454	12.055029136758266	43.82767191383596	24.31544282999032
51	16.98147361678723	11.822492045699805	40.5248097582032	30.67122457930977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	486.0
2	705.0
3	10400.0
4	20095.0
5	12075.5
6	4056.0
7	3719.0
8	3382.0
9	3274.0
10	3166.0
11	3097.0
12	3028.0
13	2770.0
14	2512.0
15	2325.0
16	2138.0
17	1987.5
18	1837.0
19	1631.5
20	1426.0
21	1312.0
22	1198.0
23	1061.5
24	925.0
25	852.0
26	733.5
27	688.0
28	672.0
29	656.0
30	663.0
31	670.0
32	774.5
33	879.0
34	953.0
35	1027.0
36	1213.0
37	1399.0
38	1628.0
39	1857.0
40	2805.5
41	3754.0
42	4941.5
43	6129.0
44	7017.0
45	7905.0
46	10356.5
47	12808.0
48	15771.0
49	18734.0
50	18580.5
51	18427.0
52	14769.5
53	11112.0
54	7977.5
55	4843.0
56	3509.5
57	2176.0
58	1721.0
59	1266.0
60	1162.5
61	1059.0
62	950.0
63	841.0
64	737.5
65	634.0
66	512.5
67	391.0
68	352.0
69	313.0
70	266.5
71	220.0
72	183.5
73	147.0
74	121.0
75	71.5
76	48.0
77	35.5
78	23.0
79	15.0
80	7.0
81	4.5
82	2.0
83	1.0
84	0.0
85	3.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	143633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.406703194948236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66079852721207	37.59303224189427
2	10.612990449890692	10.274797574373578
3	4.468703256242089	6.489455765736286
4	2.399033482913359	4.645172070485195
5	1.3419054193993787	3.247860867627913
6	0.805430905534461	2.3392952872947026
7	0.5508572086066046	1.8665626979872312
8	0.38257968012886895	1.4815536819533115
9	0.25745023587619376	1.1216085439975492
>10	1.389368312046945	12.142056491196312
>50	0.08341962950178346	2.7110761454540393
>100	0.038833275802554364	2.9561451755515793
>500	0.005753077896674721	2.1081506339072495
>1k	0.0014382694741686803	1.2350922141847627
>5k	0.0	0.0
>10k+	0.0014382694741686803	9.788140608356018
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14059	9.788140608356018	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	1774	1.2350922141847627	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	995	0.6927377413268538	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	790	0.5500128800484568	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	697	0.4852645283465499	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	546	0.38013548418538917	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	468	0.3258304150160478	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	303	0.21095430715782587	No Hit
TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	208	0.14481351778491014	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	196	0.13645889175885764	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	187	0.13019292223931828	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	186	0.1294967034038139	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	177	0.12323073388427451	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.11348367018721324	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	160	0.11139501368070011	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	157	0.10930635717418698	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	156	0.1086101383386826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5527977553904744	0.0
2	0.0	0.0	0.0	2.0002367144040716	0.0
3	0.0	0.0	0.0	2.7897488738660337	0.0
4	0.0	0.0	0.0	3.937813733612749	0.0
5	0.0	0.0	0.0	6.486670890394269	0.0
6	0.0	0.0	0.0	8.172912909985866	0.0
7	0.0	0.0	0.0	9.817381799447203	0.0
8	0.0	0.0	0.0	12.552825604143894	0.0
9	0.0	0.0	0.0	13.811589258735806	0.0
10	0.0	0.0	0.0	16.76703821545188	0.0
11	0.0	0.0	0.0	24.121197774884603	0.0
12	0.0	0.0	0.0	30.181782737950194	0.0
13	0.0	0.0	0.0	32.253729992411216	0.0
14	0.0	0.0	0.0	33.05159677789923	0.0
15	0.0	0.0	0.0	34.147445224983116	0.0
16	6.962188355043757E-4	0.0	0.0	36.839723461878535	0.0
17	6.962188355043757E-4	0.0	0.0	39.76941232168095	0.0
18	0.0020886565065131274	0.0	0.0	42.41574011543308	0.0
19	0.0020886565065131274	0.0	0.0	44.078310694617535	0.0
20	0.0020886565065131274	0.0	0.0	45.43941851802859	0.0
21	0.0020886565065131274	0.0	0.0	47.016354180446	0.0
22	0.0020886565065131274	0.0	0.0	48.35518300112091	0.0
23	0.0020886565065131274	0.0	0.0	49.274888082822194	0.0
24	0.0020886565065131274	0.0	0.0	49.79148245876644	0.0
25	0.0020886565065131274	0.0	0.0	50.12427506213753	0.0
26	0.0020886565065131274	0.0	0.0	50.38814200079369	0.0
27	0.0020886565065131274	0.0	0.0	50.584475712405926	0.0
28	0.0020886565065131274	0.0	0.0	50.759922858953026	0.0
29	0.0020886565065131274	0.0	0.0	50.92771159830958	0.0
30	0.0020886565065131274	0.0	0.0	51.12961506060585	0.0
31	0.002784875342017503	0.0	0.0	51.2806945479103	0.0
32	0.002784875342017503	0.0	0.0	51.42202697151769	0.0
33	0.002784875342017503	0.0	0.0	51.54525770540196	0.0
34	0.003481094177521879	0.0	0.0	51.67057709579275	0.0
35	0.004177313013026255	0.0	0.0	51.79450404851253	0.0
36	0.00487353184853063	0.0	0.0	51.92260831424533	0.0
37	0.00487353184853063	0.0	0.0	52.05140879881364	0.0
38	0.00487353184853063	0.0	0.0	52.18577903406599	0.0
39	0.00487353184853063	0.0	0.0	52.339643396712454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	35	1.2020246E-7	45.0	5
TTTTGCG	40	6.7429937E-9	45.0	1
GTGTTTG	35	1.2020246E-7	45.0	1
TGTTTAG	20	7.012661E-4	45.0	1
TCTGCGG	20	7.012661E-4	45.0	2
ATAGCAA	20	7.012661E-4	45.0	10
CGGGATG	20	7.012661E-4	45.0	6
GGGATTC	20	7.012661E-4	45.0	7
TGGGTAG	20	7.012661E-4	45.0	6
TGGGTAC	35	1.2020246E-7	45.0	6
TCTTCGG	35	1.2020246E-7	45.0	2
TGCAGGG	45	3.8016879E-10	45.0	3
GACTTTG	20	7.012661E-4	45.0	1
GGTATGC	45	3.8016879E-10	45.0	8
CGTTTTG	20	7.012661E-4	45.0	1
CTCTGTG	20	7.012661E-4	45.0	1
GTATGCT	25	3.8737202E-5	44.999996	9
GCCTCGG	25	3.8737202E-5	44.999996	2
GGGTATG	60	0.0	44.999996	7
TATGCTA	25	3.8737202E-5	44.999996	10
>>END_MODULE