##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933647.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	144014
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872685988862195	33.0	31.0	34.0	30.0	34.0
2	32.12718207952005	34.0	31.0	34.0	30.0	34.0
3	32.17173330370658	33.0	31.0	34.0	30.0	34.0
4	35.81423333842543	37.0	35.0	37.0	35.0	37.0
5	35.914209729609624	37.0	35.0	37.0	35.0	37.0
6	35.91052953185107	37.0	35.0	37.0	35.0	37.0
7	35.888601108225586	37.0	35.0	37.0	35.0	37.0
8	35.74344855361284	37.0	35.0	37.0	35.0	37.0
9	37.5293652006055	39.0	37.0	39.0	35.0	39.0
10	37.14915216576166	39.0	37.0	39.0	33.0	39.0
11	37.19548099490327	39.0	37.0	39.0	34.0	39.0
12	37.229699890288444	39.0	37.0	39.0	34.0	39.0
13	37.17340675212132	39.0	37.0	39.0	33.0	39.0
14	38.2781257377755	40.0	38.0	41.0	33.0	41.0
15	38.29938755954282	40.0	38.0	41.0	33.0	41.0
16	38.45069229380477	40.0	38.0	41.0	34.0	41.0
17	38.50413848653603	40.0	38.0	41.0	34.0	41.0
18	38.06375769022456	39.0	38.0	40.0	34.0	41.0
19	37.5470995875401	38.0	37.0	40.0	34.0	41.0
20	36.81285847209299	37.0	35.0	40.0	34.0	41.0
21	36.72037440804366	37.0	35.0	40.0	33.0	41.0
22	36.714805505020344	37.0	35.0	40.0	33.0	41.0
23	36.591248073104005	37.0	35.0	40.0	33.0	41.0
24	36.42244504006555	37.0	35.0	40.0	33.0	41.0
25	36.339626702959436	37.0	35.0	40.0	33.0	41.0
26	36.352583776577276	37.0	35.0	40.0	33.0	41.0
27	36.288763592428516	37.0	35.0	40.0	33.0	41.0
28	36.20540364131265	37.0	35.0	40.0	33.0	41.0
29	36.02060216367853	37.0	35.0	40.0	32.0	41.0
30	35.7060702431708	37.0	35.0	40.0	31.0	41.0
31	35.165886649909034	36.0	35.0	40.0	29.0	41.0
32	34.630452594886606	37.0	35.0	40.0	23.0	41.0
33	33.807643701306816	37.0	34.0	40.0	18.0	41.0
34	33.21213215381838	37.0	33.0	40.0	15.0	41.0
35	32.88521949254933	37.0	33.0	40.0	10.0	41.0
36	32.52290055133529	37.0	32.0	40.0	10.0	41.0
37	32.16105378643743	36.0	31.0	40.0	10.0	41.0
38	31.979356173705334	36.0	31.0	40.0	10.0	41.0
39	32.020928520838254	36.0	31.0	40.0	9.0	41.0
40	31.89558654019748	36.0	30.0	40.0	8.0	41.0
41	31.70234143902676	36.0	30.0	40.0	8.0	41.0
42	31.277091116141488	35.0	29.0	40.0	8.0	41.0
43	31.034753565625564	35.0	27.0	40.0	8.0	41.0
44	30.919966114405543	35.0	26.0	40.0	7.0	41.0
45	30.86860305248101	35.0	26.0	40.0	7.0	41.0
46	30.377539683641867	35.0	24.0	39.0	7.0	41.0
47	30.152561556515337	35.0	23.0	39.0	7.0	40.0
48	30.049745163664642	35.0	23.0	39.0	7.0	40.0
49	30.044912300193037	35.0	24.0	39.0	7.0	40.0
50	29.81423333842543	35.0	23.0	39.0	7.0	40.0
51	28.712402960823255	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	3.0
15	10.0
16	8.0
17	31.0
18	82.0
19	139.0
20	262.0
21	454.0
22	698.0
23	1129.0
24	2069.0
25	3894.0
26	5646.0
27	5929.0
28	5285.0
29	4690.0
30	4603.0
31	4612.0
32	5058.0
33	5934.0
34	7851.0
35	12134.0
36	16041.0
37	15748.0
38	25507.0
39	16188.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.791923007485384	4.15931784409849	34.031413612565444	43.01734553585068
2	48.68207257627731	4.0114155568208645	32.22533920313303	15.081172663768799
3	14.570805616120655	4.131542766675462	64.27222353382311	17.025428083380785
4	12.381435138250447	4.275278792339634	65.04159317844098	18.301692890968933
5	10.214979099254238	5.260599663921563	66.24078214618024	18.283639090643966
6	14.155568208646383	6.258419320343855	68.68360020553558	10.90241226547419
7	50.34718846778785	2.533781437915758	42.11396114266668	5.005068951629703
8	48.28974960767703	2.5254489146888495	41.9077311928007	7.277070284833418
9	43.5027150138181	4.475953726721013	45.01020734095296	7.011123918507922
10	21.697890482869724	16.03385781937867	49.039676698098795	13.228574999652812
11	14.28055605705001	14.299304234310553	52.238671240296085	19.181468468343354
12	13.834766064410406	11.537767161525963	57.32428791645256	17.303178857611066
13	17.008763036926965	12.711958559584485	58.37626897384977	11.903009429638784
14	13.895176857805492	15.121446526032193	56.14662463371617	14.83675198244615
15	9.394919938339328	13.938228227811186	59.11925229491577	17.547599538933714
16	8.413070951435277	14.07015984557057	56.416737261655115	21.100031941339036
17	8.413070951435277	14.157651339453109	54.507895065757495	22.921382643354118
18	9.113002902495591	13.209132445456692	58.91579985279209	18.76206479925563
19	9.737247767578152	15.29990139847515	59.365061730109566	15.597789103837126
20	9.861541239046204	19.2259085922202	56.22717235824295	14.685377810490646
21	9.025511408613053	20.027219575874568	56.74101129056898	14.206257724943407
22	7.460385795825405	19.023150527032094	53.378143791575816	20.13831988556668
23	7.9054814115294345	19.25229491577208	51.11586373547016	21.726359937228327
24	9.576846695460164	18.571805518907883	50.954073909481025	20.89727387615093
25	7.9214520810476765	20.613968086436042	48.47862013415362	22.98595969836266
26	8.279750579804741	20.602163678531255	50.78117405252267	20.336911689141335
27	11.223214409710167	19.857097226658517	52.09701834543864	16.822670018192674
28	7.942283389114947	17.567042093129835	52.3039426722402	22.18673184551502
29	10.242754176677266	15.271431944116545	52.018553751718585	22.467260127487606
30	17.517046953768382	17.225408640826586	48.261280153318424	16.9962642520866
31	16.019970280667156	23.652561556515337	42.6423819906398	17.685086172177705
32	14.054883552987905	22.05063396614218	42.80417181662894	21.090310664240977
33	18.146152457399975	18.427375116308138	41.80079714472204	21.625675281569848
34	17.282347549543793	17.46635743747136	40.814781896204536	24.43651311678031
35	18.104489841265433	16.301887316510893	38.773313705611955	26.82030913661172
36	21.984668157262487	21.23890732845418	37.349146610746175	19.427277903537156
37	20.83408557501354	24.753148999402836	39.15244351243629	15.260321913147331
38	17.27957004180149	23.562292554890497	43.251350563139695	15.906786840168316
39	18.371130584526504	18.20309136611718	47.32803755190468	16.09774049745164
40	24.615662366158848	15.263099420889636	43.20413293152055	16.91710528143097
41	26.887663699362562	18.79886677684114	35.24101823433833	19.07245128945797
42	23.35328509728221	23.37411640534948	36.302026191898015	16.970572305470302
43	18.346827391781353	21.90342605580013	43.0597025289208	16.690044023497716
44	16.28383351618593	17.946171899954173	43.009707389559345	22.760287194300556
45	21.86801283208577	14.115988723318566	41.3730609524074	22.64293749218826
46	24.823975446831557	15.444331801074895	43.78740955740414	15.944283194689405
47	17.578152124099045	21.404169039121197	45.32614884663991	15.691529990139847
48	16.1588456677823	19.648784145985807	46.978765953310095	17.2136042329218
49	20.452872637382477	14.562473092893747	47.4245559459497	17.560098323774078
50	19.930006804893967	11.961337092227145	43.60062216173428	24.50803394114461
51	17.137917146944048	12.05924424014332	40.253725332259364	30.549113280653266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	448.5
2	667.0
3	10185.0
4	19703.0
5	11918.5
6	4134.0
7	3781.5
8	3429.0
9	3338.0
10	3247.0
11	3106.5
12	2966.0
13	2742.0
14	2518.0
15	2338.0
16	2158.0
17	1963.5
18	1769.0
19	1581.5
20	1394.0
21	1294.5
22	1195.0
23	1053.0
24	911.0
25	856.5
26	739.0
27	676.0
28	665.5
29	655.0
30	663.0
31	671.0
32	801.0
33	931.0
34	1020.0
35	1109.0
36	1264.5
37	1420.0
38	1628.0
39	1836.0
40	2814.5
41	3793.0
42	5016.5
43	6240.0
44	7107.0
45	7974.0
46	10270.0
47	12566.0
48	15821.0
49	19076.0
50	18860.0
51	18644.0
52	14853.0
53	11062.0
54	7985.0
55	4908.0
56	3537.0
57	2166.0
58	1740.0
59	1314.0
60	1206.5
61	1099.0
62	986.0
63	873.0
64	748.5
65	624.0
66	506.0
67	388.0
68	353.5
69	319.0
70	272.5
71	226.0
72	191.0
73	156.0
74	116.0
75	55.0
76	34.0
77	30.5
78	27.0
79	21.5
80	16.0
81	11.0
82	6.0
83	3.5
84	1.0
85	2.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	144014.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.961906481314315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0207624234173	38.200452733762
2	10.460630814613117	10.243448553612842
3	4.448888132516451	6.534781340702987
4	2.2832992965736327	4.471787465107559
5	1.3316882232811438	3.260099712527949
6	0.7941910596777852	2.3331065035343785
7	0.5445881552076242	1.8664852028275027
8	0.3715679600635353	1.4554140569666838
9	0.2822214658497844	1.243629091616093
>10	1.33452461992285	11.852319913341757
>50	0.08509189925119127	2.8969405752218536
>100	0.03403675970047651	2.7559820572999847
>500	0.005672793283412752	2.1122946380213037
>1k	0.001418198320853188	1.2276584220978517
>5k	0.0	0.0
>10k+	0.001418198320853188	9.545599733359257
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13747	9.545599733359257	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	1768	1.2276584220978517	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	984	0.6832669046064965	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	843	0.5853597566903217	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	689	0.4784257086116628	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	526	0.36524226811282234	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	485	0.33677281375421836	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	343	0.23817128890246783	No Hit
TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	230	0.15970669518241282	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	191	0.1326259946949602	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	182	0.12637660227477884	No Hit
CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	177	0.1229047175969003	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	168	0.11665532517671892	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	160	0.11110030969211328	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATG	157	0.10901717888538613	No Hit
GCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	156	0.10832280194981043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5235602094240838	0.0
2	0.0	0.0	0.0	1.9810573971974947	0.0
3	0.0	0.0	0.0	2.828891635535434	0.0
4	0.0	0.0	0.0	4.03641312650159	0.0
5	0.0	0.0	0.0	6.633382865554737	0.0
6	0.0	0.0	0.0	8.326273834488314	0.0
7	0.0	0.0	0.0	9.971252794867166	0.0
8	0.0	0.0	0.0	12.636965850542309	0.0
9	0.0	0.0	0.0	13.892399350063188	0.0
10	0.0	0.0	0.0	16.93029844320691	0.0
11	0.0	0.0	0.0	24.196953074006693	0.0
12	0.0	0.0	0.0	30.25122557529129	0.0
13	0.0	0.0	0.0	32.26561306539642	0.0
14	0.0	0.0	0.0	33.0808115877623	0.0
15	0.0	0.0	0.0	34.15431833016235	0.0
16	0.0	0.0	0.0	36.79572819309234	0.0
17	0.0	0.0	0.0	39.74821892316025	0.0
18	0.0	0.0	0.0	42.345188662213396	0.0
19	0.0	0.0	0.0	43.924896190648134	0.0
20	0.0	0.0	0.0	45.250461760662155	0.0
21	0.0	0.0	0.0	46.8586387434555	0.0
22	0.0	0.0	0.0	48.10573971974947	0.0
23	0.0	0.0	0.0	49.00634660519116	0.0
24	0.0	0.0	0.0	49.53546183009985	0.0
25	0.0	0.0	0.0	49.899315344341524	0.0
26	0.0	0.0	0.0	50.10901717888539	0.0
27	0.0	0.0	0.0	50.30483147471774	0.0
28	0.0	0.0	0.0	50.488841362645296	0.0
29	0.0	0.0	0.0	50.65826933492577	0.0
30	0.0	0.0	0.0	50.85408363075812	0.0
31	0.0	0.0	0.0	51.00198591803575	0.0
32	0.0	0.0	0.0	51.16377574402489	0.0
33	0.0	0.0	0.0	51.29987362339772	0.0
34	0.0	0.0	0.0	51.44708153373978	0.0
35	0.0	0.0	0.0	51.584568166983765	0.0
36	0.0	0.0	0.0	51.699140361353756	0.0
37	0.0	0.0	0.0	51.83662699459775	0.0
38	0.0	0.0	0.0	51.9838349049398	0.0
39	0.0	0.0	0.0	52.12201591511936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGGG	45	3.8016879E-10	45.0	3
GCGGGCA	20	7.012726E-4	45.0	5
ATGGGGT	20	7.012726E-4	45.0	4
TGCGTTG	20	7.012726E-4	45.0	1
GGGTATG	35	1.202061E-7	45.0	7
CTCTCTG	55	1.8189894E-12	45.0	1
CGAGGGA	20	7.012726E-4	45.0	4
TCTGCGG	45	3.8016879E-10	45.0	2
CAGGGAT	20	7.012726E-4	45.0	5
CGGGATA	20	7.012726E-4	45.0	6
TTCTTCG	20	7.012726E-4	45.0	1
CTTATGG	20	7.012726E-4	45.0	2
TTGATGG	20	7.012726E-4	45.0	2
GCTTGCG	35	1.202061E-7	45.0	1
GGTAGAA	20	7.012726E-4	45.0	8
TTCTACG	20	7.012726E-4	45.0	1
GGGATCA	20	7.012726E-4	45.0	7
GCTCTCG	20	7.012726E-4	45.0	1
GGTATGG	20	7.012726E-4	45.0	8
TCGAGGG	20	7.012726E-4	45.0	3
>>END_MODULE