##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933644.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95863
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926561864327216	33.0	31.0	34.0	30.0	34.0
2	32.13629867623588	33.0	31.0	34.0	30.0	34.0
3	32.22402804001544	33.0	31.0	34.0	30.0	34.0
4	35.84014687627135	37.0	35.0	37.0	35.0	37.0
5	35.86473404754702	37.0	35.0	37.0	35.0	37.0
6	35.8212449015783	37.0	35.0	37.0	35.0	37.0
7	35.89741610423208	37.0	35.0	37.0	35.0	37.0
8	35.70065614470651	37.0	35.0	37.0	35.0	37.0
9	37.52350750550264	39.0	37.0	39.0	35.0	39.0
10	37.05062432846875	39.0	37.0	39.0	33.0	39.0
11	37.09428037929128	39.0	37.0	39.0	33.0	39.0
12	37.214024180340694	39.0	37.0	39.0	34.0	39.0
13	37.13918821651732	39.0	37.0	39.0	33.0	39.0
14	38.21456662111555	40.0	37.0	41.0	33.0	41.0
15	38.228471881747915	40.0	37.0	41.0	33.0	41.0
16	38.32793674306041	40.0	38.0	41.0	34.0	41.0
17	38.31494945912396	40.0	38.0	41.0	34.0	41.0
18	37.99732952233917	39.0	37.0	40.0	34.0	41.0
19	37.65127317108791	39.0	37.0	40.0	34.0	41.0
20	37.13540156264669	38.0	35.0	40.0	33.0	41.0
21	36.99713132282528	38.0	35.0	40.0	33.0	41.0
22	37.02917705475522	38.0	35.0	40.0	33.0	41.0
23	36.97505815591	38.0	35.0	40.0	33.0	41.0
24	36.87345482615817	38.0	35.0	40.0	33.0	41.0
25	36.77384392309859	38.0	35.0	40.0	33.0	41.0
26	36.730740744604276	38.0	35.0	40.0	33.0	41.0
27	36.646464224987746	38.0	35.0	40.0	32.0	41.0
28	36.57361025630327	38.0	35.0	40.0	32.0	41.0
29	36.48139532457778	38.0	35.0	40.0	32.0	41.0
30	36.35079227647789	38.0	35.0	40.0	31.0	41.0
31	35.98029479569803	38.0	35.0	40.0	30.0	41.0
32	35.55835932528713	38.0	35.0	40.0	29.0	41.0
33	35.10735111565463	38.0	35.0	40.0	24.0	41.0
34	34.753032974140176	38.0	35.0	40.0	21.0	41.0
35	34.49680272889436	38.0	35.0	40.0	18.0	41.0
36	34.30871139021312	38.0	34.0	40.0	18.0	41.0
37	34.11848158309254	38.0	34.0	40.0	17.0	41.0
38	34.01495884752198	38.0	34.0	40.0	16.0	41.0
39	33.860446679114986	38.0	34.0	40.0	15.0	41.0
40	33.551015511719854	38.0	33.0	40.0	15.0	41.0
41	33.39457350594077	38.0	33.0	40.0	14.0	41.0
42	33.403826293773406	38.0	33.0	40.0	12.0	41.0
43	33.324515193557474	38.0	33.0	40.0	12.0	41.0
44	33.24344116082326	38.0	33.0	40.0	10.0	41.0
45	33.13967849952537	37.0	33.0	40.0	10.0	41.0
46	32.81630034528442	37.0	32.0	40.0	10.0	41.0
47	32.62550723428226	37.0	31.0	40.0	10.0	41.0
48	32.63100466290435	37.0	31.0	40.0	10.0	41.0
49	32.710086268946306	37.0	31.0	40.0	10.0	41.0
50	32.609494799870646	37.0	31.0	40.0	10.0	41.0
51	31.505659117699217	35.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	1.0
15	4.0
16	7.0
17	13.0
18	21.0
19	57.0
20	105.0
21	185.0
22	310.0
23	457.0
24	885.0
25	1640.0
26	2488.0
27	2842.0
28	2736.0
29	2497.0
30	2438.0
31	2726.0
32	3099.0
33	3848.0
34	5341.0
35	7605.0
36	10070.0
37	12111.0
38	19891.0
39	14479.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.964021572452353	4.992541439345733	38.77408384882593	33.26935313937599
2	40.0602943784359	4.946642604550243	37.16240885430249	17.830654162711372
3	19.248302264690235	5.00818876938965	56.7737291760116	18.969779789908515
4	15.645243733244317	5.2919270208526745	56.87595839896519	22.18687084693782
5	11.91283394010202	7.048600607116405	58.69835077141337	22.340214681368202
6	16.35458936190188	8.274308127223225	62.27220095344398	13.098901557430917
7	60.33297518333455	3.3099318819565418	30.75326246831416	5.603830466394751
8	58.310296986324225	3.6771225603204574	30.642688002670475	7.369892450684831
9	52.92970176188936	5.615305175093623	33.46963896393812	7.985354099078894
10	25.944316368150382	18.968736634572252	38.834586858329075	16.25236013894829
11	16.517321594358616	16.51314897301357	43.19184669789178	23.77768273473603
12	15.431396889310786	13.138541460208838	50.400050071456135	21.030011579024233
13	19.096001585596113	14.789856357510198	51.74154783388795	14.372594223005747
14	16.676924360806566	16.618507661975947	48.99074721216736	17.713820765050123
15	11.755317484326591	16.155346692676005	51.48180215515893	20.607533667838478
16	11.181582049382973	15.691142568039806	48.44100434995775	24.686271032619466
17	10.99172777818345	16.02495227564337	47.037960422686545	25.945359523486644
18	12.058875687178578	15.1466154825115	50.62328531341602	22.1712235168939
19	13.04674379061786	17.626195716804187	50.77037021582884	18.55669027674911
20	12.982068159769671	20.88918560862898	48.44413381596653	17.684612415634813
21	12.249773113714364	22.395501914190042	47.68993250784974	17.664792464245853
22	10.322022052303808	20.259119785527265	45.46905479694981	23.949803365219115
23	10.656874915243629	21.199002743498536	43.3013780081992	24.842744333058636
24	12.68163942292647	20.248688232164653	43.23357291134223	23.836099433566652
25	11.672908212761962	22.42992604028666	41.21298102500444	24.684184721946945
26	10.96460573944066	22.603089826106004	44.002378394166676	22.42992604028666
27	12.817249616640414	21.220909005560017	44.91096669205012	21.050874685749456
28	10.42529443059366	18.36266338420454	45.60257867999124	25.609463505210563
29	12.65556053951994	16.482897468262	45.200963875530704	25.660578116687354
30	18.255218384569645	18.71212042185202	41.18481583092538	21.847845362652954
31	15.997830236900576	24.787457100236796	35.22526939486559	23.98944326799704
32	13.634040244932875	23.013049873256627	36.89849055422843	26.45441932758207
33	17.45824770766615	18.148816540271014	35.85116259662227	28.541773155440573
34	16.7457726129998	17.215192514317305	37.44719026110178	28.591844611581113
35	15.073594608973224	16.694658001523006	39.75569302024765	28.476054369256126
36	16.851131301962173	17.992343239831843	37.82481249282831	27.331712965377676
37	14.559319028196487	20.04005716491243	35.49231716094844	29.908306645942645
38	16.56947936117167	18.680825761764183	36.2955467698695	28.454148107194644
39	22.527982641895207	17.436341445604665	37.850891376234834	22.184784536265294
40	22.089857400665533	20.946559152123342	35.25239143360837	21.711192013602744
41	15.316649802322063	21.819680168573903	33.87334007907118	28.990329950032862
42	15.31352033631328	17.40400363018057	39.24350375014343	28.038972283362718
43	16.203331838144017	14.978667473373461	43.69882019131469	25.119180497167832
44	18.41377799568134	15.514849316211677	39.011923265493465	27.059449422613525
45	22.350646234730814	15.236326841429957	35.52361182103627	26.88941510280296
46	23.054776086707072	17.211019892972264	37.53168584333893	22.202518176981734
47	15.166435433900464	20.724367065499724	41.751249178515174	22.357948322084642
48	14.767950095448711	18.78096867404525	42.714081553884185	23.736999676621846
49	20.382212115206073	14.25367451467198	43.35979470702983	22.00431866309212
50	20.092214931725483	12.671207869563858	39.3384308857432	27.898146312967466
51	17.62828202747671	12.90696097555887	35.73850182030606	33.726255176658356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	193.0
1	404.5
2	616.0
3	4413.5
4	8211.0
5	5047.0
6	1883.0
7	1708.5
8	1534.0
9	1497.0
10	1460.0
11	1357.5
12	1255.0
13	1224.5
14	1194.0
15	1078.0
16	962.0
17	868.5
18	775.0
19	710.0
20	645.0
21	603.5
22	562.0
23	520.0
24	478.0
25	439.5
26	414.0
27	427.0
28	441.0
29	455.0
30	474.5
31	494.0
32	556.5
33	619.0
34	669.0
35	719.0
36	802.5
37	886.0
38	1121.0
39	1356.0
40	1683.0
41	2010.0
42	2655.0
43	3300.0
44	4266.5
45	5233.0
46	7114.0
47	8995.0
48	11276.0
49	13557.0
50	13646.5
51	13736.0
52	11344.5
53	8953.0
54	6655.5
55	4358.0
56	3447.5
57	2537.0
58	2153.5
59	1770.0
60	1637.5
61	1505.0
62	1410.5
63	1316.0
64	1198.0
65	1080.0
66	925.0
67	770.0
68	666.0
69	562.0
70	497.5
71	433.0
72	343.5
73	254.0
74	218.5
75	133.5
76	84.0
77	62.5
78	41.0
79	33.0
80	25.0
81	19.5
82	14.0
83	12.0
84	10.0
85	7.5
86	5.0
87	5.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	95863.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.92850213325266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86623928545703	45.68603110689213
2	11.616725491608442	13.458790148441006
3	4.293020240582006	7.460646964939549
4	1.9376215515378519	4.489740567267872
5	0.9454008499603832	2.7382827576854467
6	0.5186198948354103	1.80257242105922
7	0.38356263055535544	1.5553446063653338
8	0.24130231218036446	1.1182625204719234
9	0.19448246056327884	1.0139469868458113
>10	0.9291939782467766	9.566777588850755
>50	0.054022905712021896	2.305373293137081
>100	0.01800763523734063	3.0355820285198667
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0018007635237340634	5.768649009524008
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5530	5.768649009524008	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	496	0.5174050467855168	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	464	0.4840240760251609	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	423	0.4412547072384549	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	409	0.42665053253079915	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	345	0.3598885910100873	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	266	0.2774793194454586	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	150	0.15647330043916838	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.15438698976664617	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	105	0.10953131030741789	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104	0.10848815497115676	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	97	0.10118606761732889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46837674598124407	0.0
2	0.0	0.0	0.0	1.9183626633842046	0.0
3	0.0	0.0	0.0	2.7288943596590967	0.0
4	0.0	0.0	0.0	3.854458967484848	0.0
5	0.0	0.0	0.0	6.350729687157715	0.0
6	0.0	0.0	0.0	8.332724826053848	0.0
7	0.0	0.0	0.0	10.17389399455473	0.0
8	0.0	0.0	0.0	12.999801800486111	0.0
9	0.0	0.0	0.0	14.29122810677738	0.0
10	0.0	0.0	0.0	17.293429164536892	0.0
11	0.0	0.0	0.0	23.93624234584772	0.0
12	0.0	0.0	0.0	30.01366533490502	0.0
13	0.0	0.0	0.0	31.957063726359493	0.0
14	0.0	0.0	0.0	32.60277687950513	0.0
15	0.0	0.0	0.0	33.734600419348446	0.0
16	0.0	0.0	0.0	36.365438177399	0.0
17	0.0	0.0	0.0	39.65867957397536	0.0
18	0.0	0.0	0.0	42.509623107977006	0.0
19	0.0	0.0	0.0	44.06705402501486	0.0
20	0.0	0.0	0.0	45.442975913543286	0.0
21	0.0	0.0	0.0	46.989975277218534	0.0
22	0.0	0.0	0.0	48.34607721435799	0.0
23	0.0	0.0	0.0	49.319341143089616	0.0
24	0.0	0.0	0.0	49.875342937316795	0.0
25	0.0	0.0	0.0	50.24774939236202	0.0
26	0.0	0.0	0.0	50.50645191575477	0.0
27	0.0	0.0	0.0	50.68378832291916	0.0
28	0.0	0.0	0.0	50.878858370799996	0.0
29	0.0	0.0	0.0	51.04993584594682	0.0
30	0.0	0.0	0.0	51.241876427818866	0.0
31	0.0	0.0	0.0	51.37331400018777	0.0
32	0.0	0.0	0.0	51.50892419390171	0.0
33	0.0	0.0	0.0	51.64557754295192	0.0
34	0.0010431553362611227	0.0	0.0	51.78536035801091	0.0
35	0.0010431553362611227	0.0	0.0	51.91471161970729	0.0
36	0.0010431553362611227	0.0	0.0	52.0378039493861	0.0
37	0.0010431553362611227	0.0	0.0	52.16298258973744	0.0
38	0.0010431553362611227	0.0	0.0	52.31319695815904	0.0
39	0.0010431553362611227	0.0	0.0	52.43107351115655	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTCG	35	1.1964221E-7	45.000004	13
GCCCTAA	20	7.0009363E-4	45.000004	40
CGCGACC	20	7.0009363E-4	45.000004	10
CGTTCAT	30	2.1446685E-6	45.000004	17
TGTGAGG	20	7.0009363E-4	45.000004	2
TGCACCA	20	7.0009363E-4	45.000004	28
AATTCTC	20	7.0009363E-4	45.000004	13
GCGATGC	20	7.0009363E-4	45.000004	9
ATTAGGG	20	7.0009363E-4	45.000004	27
GGCGCGA	20	7.0009363E-4	45.000004	8
CCTGCAC	20	7.0009363E-4	45.000004	26
TTCCGAT	20	7.0009363E-4	45.000004	13
GTACTCC	20	7.0009363E-4	45.000004	21
TCTGGAC	20	7.0009363E-4	45.000004	16
TATGCCC	35	1.1964221E-7	45.000004	10
GGCCGAT	20	7.0009363E-4	45.000004	8
AATTAGG	20	7.0009363E-4	45.000004	26
GCGCGAC	20	7.0009363E-4	45.000004	9
GACATTA	20	7.0009363E-4	45.000004	20
TGTTAGG	20	7.0009363E-4	45.000004	2
>>END_MODULE