##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933643.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96147
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86496718566362	33.0	31.0	34.0	30.0	34.0
2	32.06494222388634	33.0	31.0	34.0	30.0	34.0
3	32.173848378004514	33.0	31.0	34.0	30.0	34.0
4	35.762987924740244	37.0	35.0	37.0	35.0	37.0
5	35.85055175928526	37.0	35.0	37.0	35.0	37.0
6	35.836708373636206	37.0	35.0	37.0	35.0	37.0
7	35.806473420907565	37.0	35.0	37.0	35.0	37.0
8	35.65381135136822	37.0	35.0	37.0	33.0	37.0
9	37.48928203688103	39.0	37.0	39.0	35.0	39.0
10	37.07768313103893	39.0	37.0	39.0	33.0	39.0
11	37.118599644294676	39.0	37.0	39.0	34.0	39.0
12	37.18313623930024	39.0	37.0	39.0	34.0	39.0
13	37.166900683328656	39.0	37.0	39.0	33.0	39.0
14	38.2733210604595	40.0	38.0	41.0	34.0	41.0
15	38.24506224843209	40.0	37.0	41.0	33.0	41.0
16	38.39855637721406	40.0	38.0	41.0	34.0	41.0
17	38.44336276742904	40.0	38.0	41.0	34.0	41.0
18	38.190343952489414	39.0	38.0	40.0	34.0	41.0
19	37.89469250210615	39.0	37.0	40.0	34.0	41.0
20	37.351430621860274	39.0	35.0	40.0	34.0	41.0
21	37.247600029122076	38.0	35.0	40.0	33.0	41.0
22	37.24789124985699	39.0	35.0	40.0	33.0	41.0
23	37.13913070610627	38.0	35.0	40.0	33.0	41.0
24	37.01672439077662	38.0	35.0	40.0	33.0	41.0
25	36.949993239518655	38.0	35.0	40.0	33.0	41.0
26	36.95822022528004	38.0	35.0	40.0	33.0	41.0
27	36.90483322412556	38.0	35.0	40.0	33.0	41.0
28	36.87468147732118	38.0	35.0	40.0	33.0	41.0
29	36.70914329100232	38.0	35.0	40.0	33.0	41.0
30	36.47784122229503	38.0	35.0	40.0	32.0	41.0
31	36.11663390433399	38.0	35.0	40.0	31.0	41.0
32	35.782728530271356	38.0	35.0	40.0	30.0	41.0
33	35.256419857093825	38.0	35.0	40.0	25.0	41.0
34	34.89293477695612	38.0	35.0	40.0	21.0	41.0
35	34.67133659916586	38.0	35.0	40.0	20.0	41.0
36	34.4669620476978	38.0	35.0	40.0	18.0	41.0
37	34.31010847972376	38.0	34.0	40.0	18.0	41.0
38	34.19797809604044	38.0	34.0	40.0	16.0	41.0
39	34.10660759046044	38.0	34.0	40.0	16.0	41.0
40	33.817279790321074	38.0	33.0	40.0	15.0	41.0
41	33.65612031576648	38.0	33.0	40.0	15.0	41.0
42	33.564895420553945	38.0	33.0	40.0	12.0	41.0
43	33.49834108188503	38.0	33.0	40.0	12.0	41.0
44	33.39123425587902	38.0	33.0	40.0	10.0	41.0
45	33.31296868337026	38.0	33.0	40.0	10.0	41.0
46	33.0137601797248	37.0	33.0	40.0	10.0	41.0
47	32.93558821388083	37.0	32.0	40.0	10.0	41.0
48	32.888826484445694	37.0	32.0	40.0	10.0	41.0
49	32.9721260153723	37.0	32.0	40.0	10.0	41.0
50	32.8020011024785	37.0	32.0	40.0	10.0	41.0
51	31.866516896002995	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	1.0
14	2.0
15	5.0
16	13.0
17	20.0
18	26.0
19	59.0
20	101.0
21	187.0
22	279.0
23	470.0
24	902.0
25	1590.0
26	2409.0
27	2742.0
28	2786.0
29	2364.0
30	2400.0
31	2559.0
32	2902.0
33	3587.0
34	5086.0
35	7446.0
36	9863.0
37	11304.0
38	19554.0
39	17481.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.03660020593466	5.207650784735874	38.31320790040251	33.44254110892695
2	40.140618012002456	5.013156936773899	36.9018274101116	17.944397641112047
3	19.660519829011825	5.013156936773899	56.18480035778547	19.1415228764288
4	15.527265541306539	5.233652636067688	56.83484664108085	22.404235181544927
5	12.19798849678097	6.9216928245291065	58.595692013271346	22.28462666541858
6	16.327082488273163	8.272749019730204	62.25051223647123	13.149656255525393
7	60.67063974955017	3.2699928234890328	30.66138309047604	5.3979843364847575
8	58.47400334903845	3.7629879247402416	30.57401687000114	7.188991856220163
9	52.980332199652615	5.794252550781616	33.21164466910044	8.01377058046533
10	25.81879829843885	19.012553693823	38.61690952395811	16.551738483780046
11	16.4622921151986	16.45709174493224	43.25771994966042	23.82289619020874
12	15.61879205799453	13.078931219902856	50.31566247516823	20.986614246934383
13	19.26529168876824	14.693126150581923	51.68231978116842	14.359262379481418
14	16.498694707063144	16.635984482095125	49.30575056944054	17.559570241401186
15	12.061738795802261	16.04002204956993	51.21012616098266	20.68811299364515
16	11.273362663421635	15.564708207224353	48.61410132401427	24.54782780533974
17	10.960300373386584	15.904812422644493	46.6587621038618	26.47612510010713
18	12.101261609826619	15.160119400501316	50.647446098162185	22.091172891509874
19	13.343110029434097	17.654216980248993	50.494555212331115	18.50811777798579
20	12.996765369694321	21.016776394479287	48.34784236637649	17.638615869449907
21	12.471527972791662	22.27838622109894	47.64475230636421	17.605333499745182
22	10.290492683079036	20.246081521004296	45.648850198134106	23.81457559778256
23	10.901016152350048	21.12494409601964	42.996661362289	24.97737838934132
24	12.580735748385285	20.53106181160099	43.13603128542752	23.752171154586208
25	11.981653093700272	22.34599103456166	41.115167399918874	24.557188471819195
26	11.009183853890397	22.992917095697212	43.649827867744186	22.348071182668207
27	12.997805443747595	20.60490706938334	45.03312635859673	21.364161128272332
28	10.491226975360647	18.569482147128877	45.66341123487992	25.275879642630557
29	12.696183968298543	16.051462864155926	45.59788656952375	25.65446659802178
30	18.20961652469656	18.637086960591596	41.308621173827575	21.84467534088427
31	16.105546714926103	24.56862928640519	35.387479588546704	23.938344410122
32	13.941152610065839	23.14060761126192	36.92158881712378	25.99665096154846
33	17.83831008767824	18.23665845008165	35.60901536189377	28.316016100346346
34	16.728551072836385	17.558530167347914	37.20240881150738	28.51050994830832
35	15.039470810321696	16.783674997659833	39.882679646790855	28.29417454522762
36	16.889762551093636	17.807107866080067	37.72556606030349	27.5775635225228
37	14.355102083268328	19.631397755520194	36.08432920423934	29.929170956972133
38	16.742072035528928	18.517478444465247	36.454595567204386	28.28585395280144
39	22.756820285604334	17.281870469177406	37.99182501794128	21.96948422727698
40	22.027728374260246	21.041738171757828	35.398920403132706	21.53161305084922
41	15.513744578613997	21.875877562482447	34.06034509657088	28.550032762332677
42	15.2859683609473	17.451402539860837	39.56857728270253	27.694051816489335
43	16.394687301735882	14.855377702892447	43.585343276441286	25.16459171893039
44	18.497717037453064	15.299489323639841	39.017338034468054	27.185455604439035
45	22.583127918707813	15.183001029673312	35.55389143707032	26.679979614548554
46	22.769301174243605	17.276670098911044	37.7557282078484	22.198300518996952
47	15.396216210594194	20.70579425255078	41.786015164279696	22.111974372575325
48	14.809614444548453	18.817019771807754	43.21611698752951	23.15724879611428
49	20.52482136728135	14.357182231374873	43.10170884166953	22.01628755967425
50	19.876855232092524	12.826193224957617	39.46665002548181	27.830301517468044
51	18.049445120492578	13.087251812329036	35.70782239695466	33.15548067022372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	392.0
2	616.0
3	4436.0
4	8256.0
5	5086.0
6	1916.0
7	1722.5
8	1529.0
9	1483.0
10	1437.0
11	1361.5
12	1286.0
13	1220.5
14	1155.0
15	1051.5
16	948.0
17	866.0
18	784.0
19	736.0
20	688.0
21	625.0
22	562.0
23	532.5
24	503.0
25	452.5
26	414.0
27	426.0
28	433.0
29	440.0
30	481.0
31	522.0
32	552.0
33	582.0
34	676.5
35	771.0
36	807.5
37	844.0
38	1083.0
39	1322.0
40	1692.5
41	2063.0
42	2753.5
43	3444.0
44	4228.5
45	5013.0
46	6961.0
47	8909.0
48	11207.5
49	13506.0
50	13589.5
51	13673.0
52	11337.0
53	9001.0
54	6804.0
55	4607.0
56	3553.5
57	2500.0
58	2204.0
59	1908.0
60	1728.0
61	1548.0
62	1406.0
63	1264.0
64	1204.5
65	1145.0
66	976.0
67	807.0
68	689.5
69	572.0
70	524.0
71	476.0
72	358.5
73	241.0
74	198.0
75	114.5
76	74.0
77	58.5
78	43.0
79	31.5
80	20.0
81	14.5
82	9.0
83	6.0
84	3.0
85	4.5
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	96147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.37935661019065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20719757705326	46.24065233444622
2	11.391412791733476	13.300466993249918
3	4.238375200427579	7.423008518206496
4	1.9419205415998575	4.534722872268506
5	0.9157313379654374	2.672990316910564
6	0.5451630144307857	1.9095759618084809
7	0.343844646356672	1.4051400459712733
8	0.2494209869944771	1.1648829396653042
9	0.18706574024585784	0.9828699803426004
>10	0.9014787101371815	9.244178185486806
>50	0.05522893283449136	2.187275734032263
>100	0.019597363263851773	2.6792307612301993
>500	0.0017815784785319793	0.536678211488658
>1k	0.0	0.0
>5k	0.0017815784785319793	5.718327144892717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5498	5.718327144892717	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	516	0.536678211488658	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	432	0.4493119910137602	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	409	0.4253902877884906	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	382	0.39730828835013055	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	348	0.3619457705388624	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	264	0.27457955006396456	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156	0.16225155231052452	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	144	0.14977066367125338	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	120	0.12480888639271115	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	110	0.11440814585998522	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	106	0.11024784964689487	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	105	0.10920777559362226	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4638730277595765	0.0
2	0.0	0.0	0.0	1.7868472235223147	0.0
3	0.0	0.0	0.0	2.597064911021665	0.0
4	0.0	0.0	0.0	3.779629109592603	0.0
5	0.0	0.0	0.0	6.294528170405733	0.0
6	0.0	0.0	0.0	8.295630648902202	0.0
7	0.0	0.0	0.0	10.00759254058889	0.0
8	0.0	0.0	0.0	12.673302339126545	0.0
9	0.0	0.0	0.0	13.884988611189117	0.0
10	0.0	0.0	0.0	16.896002995413273	0.0
11	0.0	0.0	0.0	23.602400490914953	0.0
12	0.0	0.0	0.0	29.568265260486548	0.0
13	0.0	0.0	0.0	31.665054551884094	0.0
14	0.0	0.0	0.0	32.32030120544583	0.0
15	0.0	0.0	0.0	33.40925873922223	0.0
16	0.0	0.0	0.0	35.928318096248454	0.0
17	0.0	0.0	0.0	39.15254766139349	0.0
18	0.0	0.0	0.0	41.825537978304055	0.0
19	0.0	0.0	0.0	43.35652698472131	0.0
20	0.0	0.0	0.0	44.67534088427096	0.0
21	0.0	0.0	0.0	46.17096737287695	0.0
22	0.0	0.0	0.0	47.44297794002933	0.0
23	0.0	0.0	0.0	48.4497696235972	0.0
24	0.0	0.0	0.0	49.002048945884944	0.0
25	0.0	0.0	0.0	49.37023516074345	0.0
26	0.0	0.0	0.0	49.602171674623236	0.0
27	0.0	0.0	0.0	49.79354530042539	0.0
28	0.0	0.0	0.0	49.96931781542846	0.0
29	0.0	0.0	0.0	50.15133077475116	0.0
30	0.0	0.0	0.0	50.358305511352405	0.0
31	0.0	0.0	0.0	50.49559528638439	0.0
32	0.0	0.0	0.0	50.64432587600237	0.0
33	0.0	0.0	0.0	50.77433513266145	0.0
34	0.0	0.0	0.0	50.906424537427064	0.0
35	0.0	0.0	0.0	51.04475438651232	0.0
36	0.0	0.0	0.0	51.16228275453212	0.0
37	0.0	0.0	0.0	51.28501149281829	0.0
38	0.0	0.0	0.0	51.40566008299791	0.0
39	0.0	0.0	0.0	51.56479141314862	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTTG	25	3.8640916E-5	45.000004	1
ATCCTAG	30	2.1447286E-6	45.000004	14
CGATAAC	25	3.8640916E-5	45.000004	16
GGACTAA	50	2.1827873E-11	45.000004	8
ATTGGGC	25	3.8640916E-5	45.000004	4
ATTGGGA	30	2.1447286E-6	45.000004	4
ATAACGA	25	3.8640916E-5	45.000004	18
GCATGGG	30	2.1447286E-6	45.000004	3
TGGGCAC	30	2.1447286E-6	45.000004	6
TGGGTAC	30	2.1447286E-6	45.000004	6
GTTGGGT	35	1.1964585E-7	45.000004	4
CCGGGAC	25	3.8640916E-5	45.000004	5
TAGGGAC	55	1.8189894E-12	45.000004	5
TGCGGGT	30	2.1447286E-6	45.000004	4
TCTTAGG	25	3.8640916E-5	45.000004	2
GCCCTAC	20	7.0010294E-4	45.0	26
GCGGGCT	20	7.0010294E-4	45.0	5
ACTAATC	45	3.765308E-10	45.0	10
TGTTGCG	20	7.0010294E-4	45.0	1
CAGCACA	20	7.0010294E-4	45.0	45
>>END_MODULE