FastQCFastQC Report
Sat 14 Jan 2017
SRR2933640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830387
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272783.2849743553307076No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC15470.1862986776045386No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC9130.10994873474656998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG302.1647702E-645.00000427
GCGATAC302.1647702E-645.0000049
AGCTACG302.1647702E-645.0000049
CGGGTAC700.045.06
CCGTTCG207.0321554E-445.01
ACGAATG207.0321554E-445.024
CGTTTTT228700.044.4588971
AGGCACG2350.041.1702110
ATCCGGG556.002665E-1140.9090923
TTTCGCG950.040.2631571
CGTTAGG850.039.7058832
GTTTTTT261250.039.7033462
CGCGGGA3800.039.671054
CTGCGCG750.039.0000041
GAACATG10050.038.9552279
CACGACC8800.038.86363627
ATGTGCG356.246195E-638.57142612
GGGTACG356.246195E-638.5714267
CACGCCG356.246195E-638.57142626
CGCTAGG356.246195E-638.5714262
Produced by FastQC (version 0.11.5)