##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933640.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830387
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50299799972784	34.0	31.0	34.0	31.0	34.0
2	32.68443027166851	34.0	31.0	34.0	31.0	34.0
3	32.73137223969065	34.0	31.0	34.0	31.0	34.0
4	36.22195675028631	37.0	37.0	37.0	35.0	37.0
5	36.243723709547474	37.0	35.0	37.0	35.0	37.0
6	36.218101921152424	37.0	37.0	37.0	35.0	37.0
7	36.314454585632966	37.0	37.0	37.0	35.0	37.0
8	36.22824538438102	37.0	37.0	37.0	35.0	37.0
9	38.072050742605555	39.0	39.0	39.0	37.0	39.0
10	37.43192872720792	39.0	37.0	39.0	34.0	39.0
11	37.39533735475146	39.0	37.0	39.0	34.0	39.0
12	37.45807316347679	39.0	37.0	39.0	35.0	39.0
13	37.4958699979648	39.0	37.0	39.0	35.0	39.0
14	38.862706183984095	40.0	38.0	41.0	35.0	41.0
15	38.909911884458694	40.0	38.0	41.0	35.0	41.0
16	38.90880878433791	40.0	38.0	41.0	35.0	41.0
17	38.805436501293975	40.0	38.0	41.0	35.0	41.0
18	38.34688765599654	39.0	38.0	40.0	35.0	41.0
19	37.82729739266149	38.0	37.0	40.0	34.0	41.0
20	37.27381690705659	38.0	35.0	40.0	34.0	41.0
21	37.16568178451734	38.0	35.0	40.0	34.0	41.0
22	37.178707036598595	38.0	35.0	40.0	34.0	41.0
23	37.06358601471362	38.0	35.0	40.0	33.0	41.0
24	36.95931053833935	38.0	35.0	40.0	33.0	41.0
25	36.88347360929301	38.0	35.0	40.0	33.0	41.0
26	36.831350924328056	38.0	35.0	40.0	33.0	41.0
27	36.7496119279324	38.0	35.0	40.0	33.0	41.0
28	36.55168734577974	38.0	35.0	40.0	33.0	41.0
29	36.34102412489598	38.0	35.0	40.0	32.0	41.0
30	36.01866960826699	38.0	35.0	40.0	31.0	41.0
31	35.47429451568967	38.0	35.0	40.0	29.0	41.0
32	34.5896889040893	38.0	35.0	40.0	21.0	41.0
33	33.58943239718348	38.0	33.0	40.0	15.0	41.0
34	32.697097859190954	38.0	32.0	40.0	10.0	41.0
35	32.13360878722813	38.0	31.0	40.0	8.0	41.0
36	31.831042634337965	38.0	30.0	40.0	7.0	41.0
37	31.663190777312266	38.0	29.0	40.0	7.0	41.0
38	31.514171103353014	37.0	27.0	40.0	7.0	41.0
39	31.436426629993004	37.0	27.0	40.0	7.0	41.0
40	31.293955709807594	37.0	25.0	40.0	7.0	41.0
41	31.174518628061374	37.0	24.0	40.0	7.0	41.0
42	31.044658695283044	37.0	24.0	40.0	7.0	41.0
43	30.887173089174084	37.0	23.0	40.0	7.0	41.0
44	30.65660589580521	37.0	23.0	40.0	7.0	41.0
45	30.62018914072595	36.0	23.0	40.0	7.0	41.0
46	30.47472925274601	36.0	23.0	40.0	7.0	41.0
47	30.375094985831907	36.0	23.0	40.0	7.0	41.0
48	30.321098475770935	36.0	23.0	40.0	7.0	41.0
49	30.281713225279297	35.0	23.0	40.0	7.0	41.0
50	30.197538015407275	35.0	22.0	40.0	7.0	41.0
51	29.41838323576838	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	2.0
12	2.0
13	8.0
14	27.0
15	31.0
16	59.0
17	146.0
18	320.0
19	666.0
20	1277.0
21	2054.0
22	3639.0
23	6676.0
24	13695.0
25	27940.0
26	43780.0
27	44100.0
28	34481.0
29	25599.0
30	20848.0
31	19353.0
32	20090.0
33	23539.0
34	35071.0
35	45544.0
36	58314.0
37	78849.0
38	146949.0
39	177250.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.20217802060967	3.7751072692611998	34.43322210005696	39.58949261007217
2	44.07655707519506	3.648660203013775	37.514676891618	14.76010583017316
3	17.303739099961827	4.19563408386692	63.88515234462967	14.615474471541582
4	13.191560079818204	4.0154771209086855	64.9173216825408	17.875641116732318
5	13.250809562288426	4.7188840865765	64.86385263738474	17.166453713750336
6	15.347301920670722	5.665189845216748	66.5494522433516	12.438055990760935
7	63.08624773750071	1.5106209514358968	33.02701029760822	2.376121013455172
8	64.5235293905131	1.6049143351232618	31.64416109597091	2.2273951783927255
9	60.34921066924217	3.65793298787192	32.9952178923803	2.9976384505056073
10	24.037226016303244	22.790457943103636	40.47630803468744	12.696008005905679
11	19.916858043297882	20.988165758857015	44.56355891891371	14.531417278931391
12	14.849100479655872	20.93228819815339	48.612393980156234	15.606217342034498
13	16.141028219372412	20.73888439968352	50.16516395367461	12.954923427269454
14	16.31143069436299	20.679032788326406	49.78642488381923	13.223111633491373
15	15.5065047983651	19.28221419651319	49.60337770220391	15.607903302917798
16	16.176794675253827	19.781138192192316	49.08060940260385	14.961457729950013
17	15.491812853524923	19.8820549936355	49.186222809364786	15.439909343474788
18	18.069406192534323	16.855393930781673	49.20537050796797	15.869829368716031
19	16.763629488419255	19.800647168127632	48.09480398898345	15.340919354469662
20	19.299916785787833	20.26705620391456	46.87537256724876	13.557654443048845
21	17.830120172883245	22.2557674915431	46.70798073669265	13.206131598881004
22	18.101559875094384	18.498724088888675	46.969425099381375	16.43029093663557
23	15.565633855057943	22.57031962205574	46.33454040104192	15.529506121844394
24	14.96651561259991	21.035493089366767	48.625159112558364	15.372832185474966
25	15.709783510580005	23.182925551580166	46.12608338039974	14.981207557440085
26	16.355024825774006	22.307550575815853	46.5547991478672	14.782625450542938
27	16.480990188911917	21.40194872992954	46.44244189757306	15.674619183585484
28	14.419662157524144	23.164741259196013	46.750250184552506	15.665346398727339
29	16.31937879566997	20.933853733259312	46.43967210469335	16.307095366377364
30	17.52002379613361	20.59124239661748	46.88067130145342	15.008062505795492
31	17.945006364502333	22.576220485147285	43.43878215819853	16.03999099215185
32	16.659099913654718	22.47807347658381	43.458652411465984	17.404174198295493
33	17.561450263551816	22.747104663247377	39.94414652445185	19.747298548748958
34	17.837466145303335	25.375638106087884	38.47579502087581	18.311100727732974
35	18.03135164688272	26.323629825611434	36.72095059291631	18.924067934589534
36	18.70621770331183	28.497435533070725	34.63276761317314	18.163579150444313
37	20.4516689206358	25.912616647418613	34.324959326193685	19.310755105751895
38	21.091731927402524	27.29028754062865	33.93369597549095	17.684284556477884
39	21.759613288743683	26.201638513127012	32.366836186019285	19.671912012110017
40	21.710599997350634	26.849167918091204	32.865519330143655	18.574712754414506
41	19.177805047526032	27.18660094630576	33.38262761820693	20.252966387961276
42	22.20338227838345	26.63902493656572	33.604933603247645	17.552659181803186
43	21.934953220606776	25.326022685807942	33.80953699901371	18.929487094571567
44	21.51900258554144	26.658052209391524	32.54374165298831	19.27920355207873
45	21.972044360039355	25.298445182788264	31.997490326799433	20.73202013037295
46	20.97419636868111	26.076997833540265	32.4720883154481	20.47671748233053
47	19.388791009493165	27.523190994078668	34.38240242200323	18.705615574424936
48	20.213707584535882	26.571104798124246	34.50848821091853	18.706699406421343
49	21.56885885737614	25.4405476000949	33.645517090224196	19.34507645230477
50	19.831957870246043	25.385031316723406	33.583618240651646	21.1993925723789
51	19.394812298362087	23.915595981150958	32.72654798304887	23.963043737438085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	373.0
1	1127.0
2	1881.0
3	25540.0
4	49199.0
5	34107.5
6	19016.0
7	18826.0
8	18636.0
9	18940.0
10	19244.0
11	19069.0
12	18894.0
13	18234.0
14	17574.0
15	17014.0
16	16454.0
17	15769.5
18	15085.0
19	14360.0
20	13635.0
21	13097.5
22	12560.0
23	12085.0
24	11610.0
25	11478.0
26	11385.5
27	11425.0
28	12040.5
29	12656.0
30	13871.5
31	15087.0
32	16203.0
33	17319.0
34	18742.5
35	20166.0
36	22332.0
37	24498.0
38	26696.5
39	28895.0
40	31299.0
41	33703.0
42	36678.0
43	39653.0
44	42219.5
45	44786.0
46	48625.0
47	52464.0
48	56385.5
49	60307.0
50	60274.0
51	60241.0
52	56072.5
53	51904.0
54	45704.5
55	39505.0
56	34043.0
57	28581.0
58	24402.5
59	20224.0
60	17229.5
61	14235.0
62	12018.0
63	9801.0
64	7793.5
65	5786.0
66	4621.0
67	3456.0
68	2793.5
69	2131.0
70	1706.0
71	1281.0
72	1402.0
73	1523.0
74	1882.5
75	1915.0
76	1588.0
77	1183.5
78	779.0
79	559.5
80	340.0
81	288.5
82	237.0
83	145.5
84	54.0
85	31.5
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	830387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.42372711433972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75888293677158	34.54339396299028
2	7.762395471337031	6.896690763445255
3	3.5420014604111456	4.720467189476924
4	2.1785939215854357	3.8712504746188245
5	1.5886415103599796	3.5286688469372116
6	1.2013801782204963	3.202187111870649
7	0.9557962055705612	2.972202086923157
8	0.7576710806359781	2.692685866287966
9	0.6435227317474596	2.572891040432123
>10	3.479085016223843	24.938708621951903
>50	0.08946570950477181	2.7654570614350336
>100	0.04012381585685147	3.2705538625008477
>500	0.0018977480471280587	0.5532819429824578
>1k	2.7110686387543697E-4	0.18631414144402747
>5k	0.0	0.0
>10k+	2.7110686387543697E-4	3.2852470267034137
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27278	3.2849743553307076	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	1547	0.1862986776045386	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	913	0.10994873474656998	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07960143884718812	0.0
2	0.0	0.0	0.0	0.25686818314833926	0.0
3	0.0	0.0	0.0	0.3948761240240996	0.0
4	0.0	0.0	0.0	0.612967206856562	0.0
5	0.0	0.0	0.0	1.1218865420581006	0.0
6	0.0	0.0	0.0	1.8104811371083602	0.0
7	0.0	0.0	0.0	2.348543510435496	0.0
8	0.0	0.0	0.0	3.1646690037295864	0.0
9	0.0	0.0	0.0	3.6090401222562494	0.0
10	0.0	0.0	0.0	4.492483625104921	0.0
11	0.0	0.0	0.0	6.0140633222822615	0.0
12	0.0	0.0	0.0	7.27492121143515	0.0
13	1.2042577737849942E-4	0.0	0.0	7.760598371602638	0.0
14	1.2042577737849942E-4	0.0	0.0	7.943043424331065	0.0
15	1.2042577737849942E-4	0.0	0.0	8.163061319601583	0.0
16	1.2042577737849942E-4	0.0	0.0	8.696667939165714	0.0
17	1.2042577737849942E-4	0.0	0.0	9.399834053278772	0.0
18	1.2042577737849942E-4	0.0	0.0	10.101916335395424	0.0
19	1.2042577737849942E-4	0.0	0.0	10.52593549754512	0.0
20	1.2042577737849942E-4	0.0	0.0	10.913826926481267	0.0
21	1.2042577737849942E-4	0.0	0.0	11.388424915129933	0.0
22	1.2042577737849942E-4	0.0	0.0	11.806302362633327	0.0
23	1.2042577737849942E-4	0.0	0.0	12.157343503691653	0.0
24	1.2042577737849942E-4	0.0	0.0	12.4007239997736	0.0
25	1.2042577737849942E-4	0.0	0.0	12.580037982290186	0.0
26	1.2042577737849942E-4	0.0	0.0	12.751163011945033	0.0
27	1.2042577737849942E-4	0.0	0.0	12.918554842501146	0.0
28	1.2042577737849942E-4	0.0	0.0	13.092931368145214	0.0
29	1.2042577737849942E-4	0.0	0.0	13.27706238175694	0.0
30	1.2042577737849942E-4	0.0	0.0	13.524176076937621	0.0
31	2.4085155475699884E-4	0.0	0.0	13.709752199877888	0.0
32	2.4085155475699884E-4	0.0	0.0	14.032613709029645	0.0
33	2.4085155475699884E-4	0.0	0.0	14.228185171492328	0.0
34	3.6127733213549827E-4	0.0	0.0	14.401959568249502	0.0
35	3.6127733213549827E-4	0.0	0.0	14.57717907433522	0.0
36	4.817031095139977E-4	0.0	0.0	14.745775162665119	0.0
37	0.0013246835511634936	0.0	0.0	14.914491676772396	0.0
38	0.001445109328541993	0.0	0.0	15.113314635224299	0.0
39	0.001445109328541993	0.0	0.0	15.326588686961621	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGG	30	2.1647702E-6	45.000004	27
GCGATAC	30	2.1647702E-6	45.000004	9
AGCTACG	30	2.1647702E-6	45.000004	9
CGGGTAC	70	0.0	45.0	6
CCGTTCG	20	7.0321554E-4	45.0	1
ACGAATG	20	7.0321554E-4	45.0	24
CGTTTTT	22870	0.0	44.458897	1
AGGCACG	235	0.0	41.17021	10
ATCCGGG	55	6.002665E-11	40.909092	3
TTTCGCG	95	0.0	40.263157	1
CGTTAGG	85	0.0	39.705883	2
GTTTTTT	26125	0.0	39.703346	2
CGCGGGA	380	0.0	39.67105	4
CTGCGCG	75	0.0	39.000004	1
GAACATG	1005	0.0	38.955227	9
CACGACC	880	0.0	38.863636	27
ATGTGCG	35	6.246195E-6	38.571426	12
GGGTACG	35	6.246195E-6	38.571426	7
CACGCCG	35	6.246195E-6	38.571426	26
CGCTAGG	35	6.246195E-6	38.571426	2
>>END_MODULE