FastQCFastQC Report
Sat 14 Jan 2017
SRR2933639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841774
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270473.2130952013248213No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC14590.17332443149824062No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC9170.10893660293618002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.0322084E-445.0000041
TTACTCG207.0322084E-445.0000041
CGATTTA207.0322084E-445.0000048
TTATCCG207.0322084E-445.0000041
GACGGGT207.0322084E-445.0000044
ACCGATA253.889913E-545.011
GCGATAT453.8380676E-1045.09
CGTTTTT229350.044.342711
GGCGATA700.041.7857138
TGCTGCG950.040.2631571
AGTGCGC1300.039.80769313
CTTCTCG850.039.7058831
GTTTTTT263200.039.4604872
GCCGATC800.039.3750049
AGGCACG2400.039.37499610
TGGGAAC12150.039.0740746
GGAACAT8900.038.9325878
GCGGGTC1050.038.571435
GCGTTTG2000.038.251
GCTTTCG1300.038.0769271
Produced by FastQC (version 0.11.5)