##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933639.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841774
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37872992038243	34.0	31.0	34.0	31.0	34.0
2	32.599075286240726	34.0	31.0	34.0	31.0	34.0
3	32.67137378916431	34.0	31.0	34.0	31.0	34.0
4	36.13857995138838	37.0	35.0	37.0	35.0	37.0
5	36.21065511645644	37.0	35.0	37.0	35.0	37.0
6	36.20986630615818	37.0	37.0	37.0	35.0	37.0
7	36.23377771230758	37.0	37.0	37.0	35.0	37.0
8	36.186177049897005	37.0	37.0	37.0	35.0	37.0
9	38.04007488945964	39.0	39.0	39.0	35.0	39.0
10	37.43948613285751	39.0	37.0	39.0	34.0	39.0
11	37.40373425646314	39.0	37.0	39.0	34.0	39.0
12	37.44030345437136	39.0	37.0	39.0	35.0	39.0
13	37.49171511593373	39.0	37.0	39.0	35.0	39.0
14	38.88528156013372	40.0	38.0	41.0	35.0	41.0
15	38.91578380895585	40.0	38.0	41.0	35.0	41.0
16	38.97259715790699	40.0	38.0	41.0	35.0	41.0
17	38.889494092238536	40.0	38.0	41.0	35.0	41.0
18	38.47581179746583	39.0	38.0	41.0	35.0	41.0
19	37.98927621903266	39.0	37.0	41.0	35.0	41.0
20	37.39496111782972	38.0	35.0	41.0	34.0	41.0
21	37.313472499744584	38.0	35.0	41.0	34.0	41.0
22	37.30793182017976	38.0	35.0	41.0	34.0	41.0
23	37.136483189074504	38.0	35.0	40.0	34.0	41.0
24	37.03887741840447	38.0	35.0	40.0	33.0	41.0
25	36.9710207252778	38.0	35.0	40.0	33.0	41.0
26	36.95155706876192	38.0	35.0	40.0	33.0	41.0
27	36.875442814817276	38.0	35.0	40.0	33.0	41.0
28	36.741068267729815	38.0	35.0	40.0	33.0	41.0
29	36.477574740963725	38.0	35.0	40.0	33.0	41.0
30	36.08035767319969	38.0	35.0	40.0	31.0	41.0
31	35.54543856189429	38.0	35.0	40.0	29.0	41.0
32	34.7434014355397	38.0	35.0	40.0	21.0	41.0
33	33.67096037653812	38.0	33.0	40.0	15.0	41.0
34	32.809779109357144	38.0	33.0	41.0	10.0	41.0
35	32.2952716524863	38.0	31.0	41.0	8.0	41.0
36	31.980984207162493	38.0	30.0	40.0	7.0	41.0
37	31.823382523100026	38.0	29.0	40.0	7.0	41.0
38	31.667738609175384	38.0	29.0	40.0	7.0	41.0
39	31.618987994402296	38.0	27.0	40.0	7.0	41.0
40	31.47870924975112	38.0	27.0	40.0	7.0	41.0
41	31.383814420497664	38.0	25.0	40.0	7.0	41.0
42	31.189376245880723	37.0	24.0	40.0	7.0	41.0
43	31.057619978759146	37.0	23.0	40.0	7.0	41.0
44	30.843826252652136	37.0	23.0	40.0	7.0	41.0
45	30.791297901812126	37.0	23.0	40.0	7.0	41.0
46	30.62380045000202	36.0	23.0	40.0	7.0	41.0
47	30.583301456210336	36.0	23.0	40.0	7.0	41.0
48	30.484754815425518	36.0	23.0	40.0	7.0	41.0
49	30.437149401145675	36.0	23.0	40.0	7.0	41.0
50	30.28975235633317	35.0	23.0	40.0	7.0	41.0
51	29.613372472896526	35.0	21.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	5.0
9	7.0
10	3.0
11	7.0
12	14.0
13	6.0
14	15.0
15	32.0
16	65.0
17	148.0
18	329.0
19	675.0
20	1328.0
21	2166.0
22	3621.0
23	6727.0
24	13670.0
25	27701.0
26	43698.0
27	44217.0
28	34754.0
29	25577.0
30	20888.0
31	18953.0
32	19524.0
33	23311.0
34	34688.0
35	45080.0
36	57757.0
37	76263.0
38	145380.0
39	195073.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.41777484217854	4.039682860245149	34.22771432712343	39.314827970452875
2	43.94326743282639	3.6702250247691186	37.54855816406779	14.837949378336704
3	17.553167477256366	4.257080879190852	63.55221235153379	14.637539292018998
4	13.546153718218903	4.127592441676745	64.47680731407718	17.849446526027176
5	13.330775243711496	4.7683820122740785	64.68577076507471	17.215071978939715
6	15.46971039732755	5.69796643754737	66.36935804622142	12.462965118903648
7	63.233837110673406	1.5611078508008087	32.77316714462552	2.4318878939002633
8	64.71000529833422	1.6095769173198506	31.415795688629018	2.2646220957169025
9	60.52265810063033	3.6830550717888646	32.7622378453124	3.0320489822684
10	24.144604133651075	22.847343823876717	40.2599747675742	12.748077274898012
11	20.079261179366434	21.04757333916229	44.396833354320755	14.476332127150519
12	14.931323609424856	21.045434997992334	48.36464419190899	15.658597200673816
13	16.15801866059061	20.751769477318142	50.013305233946404	13.076906628144847
14	16.446932311998232	20.784676171989155	49.527901788365995	13.240489727646612
15	15.64030250399751	19.395467191906615	49.38689006788045	15.57734023621542
16	16.291902577176295	19.909381853086458	48.80205375789701	14.996661811840234
17	15.602168753133263	19.96806743852863	49.02954949903418	15.400214309303923
18	18.28614331162521	16.89443960017772	48.90291218308002	15.916504905117051
19	16.897884705395985	19.840717342184483	47.777669540755596	15.483728411663938
20	19.43716484472079	20.296540401580472	46.71515157274993	13.551143180948804
21	18.0085153497257	22.30931342616902	46.46900474474146	13.213166479363819
22	18.25858246987909	18.567691565669644	46.76504620004894	16.408679764402322
23	15.758861642198498	22.74208991962213	46.03480269050838	15.46424574767099
24	15.154661464953776	21.134888936935567	48.325797660654764	15.384651937455896
25	15.847484004020082	23.321223986485684	45.951169791416696	14.880122218077535
26	16.474730747207683	22.417418451983547	46.38145155350486	14.72639924730391
27	16.628334921249646	21.521928688697916	46.18329860508878	15.66643778496366
28	14.56744922033705	23.258855702361917	46.54147075105669	15.632224326244337
29	16.397037684699217	21.08214318807661	46.24756763691917	16.273251490305
30	17.58880649675566	20.609688586247614	46.71099368714168	15.090511229855045
31	18.05033179927154	22.58135794168031	43.23904040752031	16.129269851527845
32	16.762218837835334	22.631727755905978	43.26600726560811	17.34004614065058
33	17.621594394695013	22.84496788924343	39.7675623148256	19.76587540123596
34	17.990695839976052	25.460990717223385	38.208355211731416	18.339958231069147
35	17.996516879827603	26.299933236236804	36.6745706092134	19.028979274722193
36	18.701456685523667	28.56051624307712	34.540506121595584	18.19752094980363
37	20.436601748212706	25.920852865495963	34.294359293587114	19.348186092704218
38	21.14605582971201	27.357699334975894	33.84114976228774	17.655095073024352
39	21.772708589241294	26.187432731350697	32.33100570937093	19.70885297003709
40	21.696322290781136	26.899025154019963	32.91346608472108	18.491186470477825
41	19.169753401744412	27.218231972001988	33.286369025415375	20.32564560083823
42	22.181369346166548	26.66463920244626	33.564353377509875	17.58963807387731
43	21.910631594703567	25.514092856277337	33.806342319910094	18.768933229109
44	21.55032110756569	26.785217885085544	32.494707605604354	19.169753401744412
45	22.036556130267744	25.33767970975582	31.917593083179096	20.708171076797335
46	21.08131161095496	26.113422367523825	32.3996702202729	20.405595801248317
47	19.43954077935408	27.757212743563	34.25171126691963	18.551535210163298
48	20.167051964066367	26.855901940425813	34.299348756317016	18.677697339190804
49	21.630033714512447	25.623979833066834	33.59013226828104	19.155854184139688
50	19.996816247591394	25.525497342517113	33.47121673988505	21.00646967000644
51	19.445480615937292	24.06904941231257	32.62859152219004	23.856878449560096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	1183.5
2	2000.0
3	25556.0
4	49112.0
5	34266.0
6	19420.0
7	19130.0
8	18840.0
9	19080.0
10	19320.0
11	19075.0
12	18830.0
13	18397.5
14	17965.0
15	17201.5
16	16438.0
17	15785.5
18	15133.0
19	14420.5
20	13708.0
21	13200.5
22	12693.0
23	12176.5
24	11660.0
25	11534.0
26	11581.5
27	11755.0
28	12298.0
29	12841.0
30	14198.5
31	15556.0
32	16415.5
33	17275.0
34	18901.5
35	20528.0
36	22710.5
37	24893.0
38	27157.0
39	29421.0
40	31788.0
41	34155.0
42	37117.5
43	40080.0
44	42716.5
45	45353.0
46	49308.5
47	53264.0
48	57270.5
49	61277.0
50	60906.0
51	60535.0
52	56497.0
53	52459.0
54	46733.5
55	41008.0
56	35067.0
57	29126.0
58	24984.5
59	20843.0
60	17689.0
61	14535.0
62	12307.5
63	10080.0
64	8007.0
65	5934.0
66	4698.0
67	3462.0
68	2837.5
69	2213.0
70	1755.5
71	1298.0
72	1437.0
73	1576.0
74	1980.5
75	2006.0
76	1627.0
77	1217.5
78	808.0
79	571.5
80	335.0
81	267.0
82	199.0
83	123.0
84	47.0
85	26.5
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	841774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.436839807146015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88981615060847	34.61177282892643
2	7.622592524622263	6.774478458635764
3	3.5198832693426296	4.692374669388955
4	2.2260551086530094	3.956754170603712
5	1.5886878237021957	3.529813316270895
6	1.2179895862907841	3.2474164879665377
7	0.933254256645932	2.902960893133869
8	0.7538491699831342	2.679893984423242
9	0.6331541550595207	2.5321832785447933
>10	3.4859671187704966	25.207953849916258
>50	0.08592382035313445	2.659918216584821
>100	0.04067219755517659	3.299898812947314
>500	0.0016161138099930654	0.49265083472557897
>1k	2.693523016655109E-4	0.17463011852881918
>5k	0.0	0.0
>10k+	2.693523016655109E-4	3.2373000794029965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27047	3.2130952013248213	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	1459	0.17332443149824062	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	917	0.10893660293618002	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.375934633286369E-4	0.0	0.0	0.08078177753173654	0.0
2	2.375934633286369E-4	0.0	0.0	0.258145297906564	0.0
3	2.375934633286369E-4	0.0	0.0	0.390960043907272	0.0
4	2.375934633286369E-4	0.0	0.0	0.608833249779632	0.0
5	2.375934633286369E-4	0.0	0.0	1.1155013103279503	0.0
6	2.375934633286369E-4	0.0	0.0	1.7972757533494739	0.0
7	2.375934633286369E-4	0.0	0.0	2.3217633236474398	0.0
8	2.375934633286369E-4	0.0	0.0	3.121978108138289	0.0
9	2.375934633286369E-4	0.0	0.0	3.5431125218882977	0.0
10	2.375934633286369E-4	0.0	0.0	4.392984340214832	0.0
11	2.375934633286369E-4	0.0	0.0	5.833869898571351	0.0
12	3.5639019499295537E-4	0.0	0.0	7.079691223535058	0.0
13	3.5639019499295537E-4	0.0	0.0	7.565332262578792	0.0
14	3.5639019499295537E-4	0.0	0.0	7.7388942875403615	0.0
15	3.5639019499295537E-4	0.0	0.0	7.961162972484301	0.0
16	3.5639019499295537E-4	0.0	0.0	8.472107715372534	0.0
17	3.5639019499295537E-4	0.0	0.0	9.152694191077416	0.0
18	3.5639019499295537E-4	0.0	0.0	9.828885187710716	0.0
19	3.5639019499295537E-4	0.0	0.0	10.259404543262205	0.0
20	3.5639019499295537E-4	0.0	0.0	10.634683418589788	0.0
21	3.5639019499295537E-4	0.0	0.0	11.07019223687118	0.0
22	3.5639019499295537E-4	0.0	0.0	11.472556767018226	0.0
23	4.751869266572738E-4	0.0	0.0	11.818374052893057	0.0
24	7.127803899859107E-4	0.0	0.0	12.05668029661168	0.0
25	7.127803899859107E-4	0.0	0.0	12.242003198008016	0.0
26	7.127803899859107E-4	0.0	0.0	12.402616379218175	0.0
27	8.315771216502292E-4	0.0	0.0	12.556695740186797	0.0
28	8.315771216502292E-4	0.0	0.0	12.734534447488281	0.0
29	8.315771216502292E-4	0.0	0.0	12.916174650203024	0.0
30	8.315771216502292E-4	0.0	0.0	13.167429737673057	0.0
31	8.315771216502292E-4	0.0	0.0	13.3490699403878	0.0
32	8.315771216502292E-4	0.0	0.0	13.667445181248173	0.0
33	8.315771216502292E-4	0.0	0.0	13.855025220546132	0.0
34	8.315771216502292E-4	0.0	0.0	14.025498530484429	0.0
35	8.315771216502292E-4	0.0	0.0	14.195140263301075	0.0
36	8.315771216502292E-4	0.0	0.0	14.362643654947766	0.0
37	0.0014255607799718215	0.0	0.0	14.539769581859264	0.0
38	0.0014255607799718215	0.0	0.0	14.730675929643823	0.0
39	0.0014255607799718215	0.0	0.0	14.945222827029582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.0322084E-4	45.000004	1
TTACTCG	20	7.0322084E-4	45.000004	1
CGATTTA	20	7.0322084E-4	45.000004	8
TTATCCG	20	7.0322084E-4	45.000004	1
GACGGGT	20	7.0322084E-4	45.000004	4
ACCGATA	25	3.889913E-5	45.0	11
GCGATAT	45	3.8380676E-10	45.0	9
CGTTTTT	22935	0.0	44.34271	1
GGCGATA	70	0.0	41.785713	8
TGCTGCG	95	0.0	40.263157	1
AGTGCGC	130	0.0	39.807693	13
CTTCTCG	85	0.0	39.705883	1
GTTTTTT	26320	0.0	39.460487	2
GCCGATC	80	0.0	39.375004	9
AGGCACG	240	0.0	39.374996	10
TGGGAAC	1215	0.0	39.074074	6
GGAACAT	890	0.0	38.932587	8
GCGGGTC	105	0.0	38.57143	5
GCGTTTG	200	0.0	38.25	1
GCTTTCG	130	0.0	38.076927	1
>>END_MODULE