SRR2933638.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933638.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130115
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10195	7.835376397801945	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	941	0.7232063943434653	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	716	0.5502824424547516	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	698	0.5364485263036545	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	534	0.4104061791492142	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	498	0.38273834684701996	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	456	0.3504592091611267	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	243	0.18675786803981093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	195	0.14986742497021865	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	193	0.1483303231756523	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	173	0.13295930522998886	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	172	0.13219075433270566	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	171	0.13142220343542252	No Hit
GTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	166	0.12757944894900666	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	159	0.12219959266802445	TruSeq Adapter, Index 27 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	155	0.11912538907889174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	153	0.11758828728432541	No Hit
GTGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	151	0.11605118548975905	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.10682857472236099	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCT	20	7.010218E-4	45.0	9
CTGGGTA	20	7.010218E-4	45.0	5
CGGGGTG	20	7.010218E-4	45.0	6
CTCCTGG	55	1.8189894E-12	45.0	2
ATGATCT	25	3.8716968E-5	45.0	11
GGACCGA	25	3.8716968E-5	45.0	8
CGCTTGG	40	6.7357178E-9	45.0	2
TGCTGCG	20	7.010218E-4	45.0	1
GCGCGGG	20	7.010218E-4	45.0	3
TAGTCTG	20	7.010218E-4	45.0	1
CGGGCTC	25	3.8716968E-5	45.0	6
GTTTATG	20	7.010218E-4	45.0	1
CGGGATC	20	7.010218E-4	45.0	6
GTCTTTG	35	1.2008604E-7	45.0	1
GACCGAT	25	3.8716968E-5	45.0	9
ATGCTAC	20	7.010218E-4	45.0	11
TAGGGTC	20	7.010218E-4	45.0	5
TAGGGAC	45	3.783498E-10	45.0	5
TGTATGG	20	7.010218E-4	45.0	2
GGTATGC	25	3.8716968E-5	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content