##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933638.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130115
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899611881796872	33.0	31.0	34.0	30.0	34.0
2	32.10727433424279	33.0	31.0	34.0	30.0	34.0
3	32.18163163355493	33.0	31.0	34.0	30.0	34.0
4	35.841832225339125	37.0	35.0	37.0	35.0	37.0
5	35.89071206240633	37.0	35.0	37.0	35.0	37.0
6	35.85395995849825	37.0	35.0	37.0	35.0	37.0
7	35.910071859508896	37.0	35.0	37.0	35.0	37.0
8	35.702125043230986	37.0	35.0	37.0	35.0	37.0
9	37.53481151289244	39.0	37.0	39.0	35.0	39.0
10	37.039611113245975	39.0	37.0	39.0	33.0	39.0
11	37.08919033162971	39.0	37.0	39.0	33.0	39.0
12	37.17801175882873	39.0	37.0	39.0	34.0	39.0
13	37.126803212542754	39.0	37.0	39.0	33.0	39.0
14	38.13340506475041	40.0	37.0	41.0	33.0	41.0
15	38.161603197171736	40.0	37.0	41.0	33.0	41.0
16	38.274157476078855	40.0	38.0	41.0	33.0	41.0
17	38.303393152211505	40.0	38.0	41.0	33.0	41.0
18	37.91738077854206	39.0	37.0	40.0	33.0	41.0
19	37.50897283172578	38.0	37.0	40.0	34.0	41.0
20	36.89562310263997	38.0	35.0	40.0	33.0	41.0
21	36.730123352419014	38.0	35.0	40.0	33.0	41.0
22	36.766998424470664	38.0	35.0	40.0	33.0	41.0
23	36.705383699035465	38.0	35.0	40.0	33.0	41.0
24	36.57892633439649	38.0	35.0	40.0	33.0	41.0
25	36.484294662414015	37.0	35.0	40.0	32.0	41.0
26	36.44799600353534	37.0	35.0	40.0	32.0	41.0
27	36.38077854205895	37.0	35.0	40.0	32.0	41.0
28	36.283364715828306	38.0	35.0	40.0	32.0	41.0
29	36.15354109825923	38.0	35.0	40.0	31.0	41.0
30	36.01155900549514	38.0	35.0	40.0	31.0	41.0
31	35.60849248741498	37.0	35.0	40.0	30.0	41.0
32	35.04095607731622	37.0	35.0	40.0	25.0	41.0
33	34.53181416439304	38.0	34.0	40.0	22.0	41.0
34	34.145832532759485	38.0	34.0	40.0	18.0	41.0
35	33.81381854513315	38.0	34.0	40.0	15.0	41.0
36	33.64951004880298	38.0	33.0	40.0	15.0	41.0
37	33.377097183260965	38.0	33.0	40.0	12.0	41.0
38	33.06468892902433	37.0	33.0	40.0	10.0	41.0
39	32.939953118395266	37.0	33.0	40.0	10.0	41.0
40	32.92522768320332	37.0	33.0	40.0	10.0	41.0
41	32.85348345694194	37.0	33.0	40.0	10.0	41.0
42	32.743742074318874	37.0	32.0	40.0	10.0	41.0
43	32.5545402144257	37.0	31.0	40.0	10.0	41.0
44	32.393544172462825	37.0	31.0	40.0	10.0	41.0
45	32.336832801752294	37.0	31.0	40.0	10.0	41.0
46	32.00648656957307	36.0	31.0	40.0	10.0	41.0
47	31.763386235253428	36.0	30.0	40.0	9.0	41.0
48	31.714245090881143	36.0	30.0	40.0	8.0	41.0
49	31.855181954424932	36.0	30.0	40.0	8.0	41.0
50	31.756430849633016	36.0	30.0	40.0	8.0	41.0
51	30.599193021557852	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	2.0
12	1.0
13	3.0
14	3.0
15	6.0
16	15.0
17	23.0
18	65.0
19	106.0
20	181.0
21	285.0
22	471.0
23	793.0
24	1505.0
25	2706.0
26	4115.0
27	4337.0
28	4043.0
29	3642.0
30	3618.0
31	3891.0
32	4313.0
33	5288.0
34	7321.0
35	11064.0
36	14505.0
37	16007.0
38	25479.0
39	16320.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.5939745609653	4.382277216308649	37.03954194366522	36.984206279060835
2	43.19332897821158	4.5836375513968415	35.89132690312416	16.331706567267418
3	17.511432194597088	4.722745263805096	59.599584982515466	18.16623755908235
4	13.504976367059909	4.604388425623487	61.4433385850978	20.447296622218804
5	11.469085040156784	5.389847442646889	61.89140375821389	21.24966375898244
6	15.189639933904623	6.923106482726818	66.32209968105138	11.565153902317181
7	56.73673289013564	2.6676401644698924	35.30722822118895	5.288398724205511
8	54.228182761403374	3.098797217845752	35.261883718249244	7.4111363025016335
9	49.15344118664259	5.054759251431426	37.84344618222342	7.948353379702571
10	24.80651731160896	17.14944472197671	42.85286093071514	15.191177035699187
11	15.008261922145794	14.58094762325635	46.99381316527687	23.416977289320986
12	13.541866810129502	12.232256081158974	54.46797064135572	19.757906467355802
13	18.080159858586633	14.192829420128348	54.683164892595016	13.043845828690003
14	15.559312915497827	16.28175075894401	52.74949083503056	15.40944549052761
15	9.988856011989395	14.979825538946317	55.461706951542865	19.569611497521425
16	8.756868923644468	14.83764362294893	52.18383737463014	24.221650078776467
17	8.565499750220958	14.700841563232526	50.47534872996964	26.258309956576873
18	10.029589209545401	13.808553971486761	54.65011720401184	21.511739614956
19	10.735887484148638	16.056565346040042	55.94128271144757	17.266264458363757
20	10.78507474157476	20.2282596164931	52.87783883487685	16.108826807055294
21	9.75060523383161	21.560158321484842	52.71721169734466	15.972024747338892
22	8.03058832571187	20.003074203589133	49.71064058717288	22.25569688352611
23	8.644660492641124	20.729354801521733	46.673327441109784	23.952657264727357
24	10.596779771740383	19.764054874534065	46.30672866310571	23.332436690619836
25	8.862160396572264	22.375590823502286	44.7181339584214	24.044114821504053
26	8.150482265688046	22.435537793490372	47.59943127233601	21.81454866848557
27	11.045613495753756	21.108250393882336	48.93440418091688	18.91173192944703
28	9.589978096299427	17.459939284479116	49.313299773277485	23.636782845943973
29	10.449986550359297	16.852015524728124	48.65311455251124	24.044883372401337
30	16.80205971640472	17.841909080428852	45.8140875379472	19.54194366521923
31	17.176344003381626	24.025669599969255	39.69181109018945	19.10617530645967
32	13.993774737732007	24.206279060830806	38.93632555816009	22.863620643277102
33	16.98420627906083	17.81962110440764	38.697306229105024	26.498866387426506
34	17.684356146485804	15.613111478307651	41.251200860777004	25.451331514429544
35	15.473235215002113	16.344771932521233	42.09814394958306	26.083848902893592
36	17.33312838642739	16.232563501517888	41.762287207470315	24.672020904584407
37	21.06367444183991	19.05775659993083	36.87430350074934	23.00426545747992
38	21.790723590669792	24.007992929331746	34.441839910848095	19.759443569150367
39	17.973331283864276	20.31433731698882	41.907543327056835	19.804788072090073
40	17.934903739000116	15.430196364754256	48.78914806133036	17.845751834915266
41	20.425008646197597	15.165814856088843	41.48714598624294	22.922030511470624
42	22.883602966606464	14.621680820812358	36.8258847942205	25.668831418360682
43	19.083118779541174	15.309533873880799	39.53733235983553	26.070014986742496
44	17.683587595588516	16.04426853168351	40.04073319755601	26.23141067517196
45	20.802367136763632	13.95842139645698	40.178303808169694	25.060907658609693
46	23.320139876263305	14.312723360104524	44.630519156131115	17.736617607501056
47	16.490796603005034	18.397571379164585	45.7641317296238	19.347500288206586
48	16.238711908696153	17.637474541751526	44.68816047342735	21.435653076124968
49	20.887676286362066	12.46128424854936	45.528955155055144	21.122084310033433
50	20.202128885985474	10.735887484148638	41.61165123160281	27.45033239826308
51	17.270875763747455	10.509164969450103	38.11551319986166	34.10444606694079
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	305.0
1	435.5
2	566.0
3	7493.0
4	14420.0
5	8637.5
6	2855.0
7	2611.0
8	2367.0
9	2289.5
10	2212.0
11	2177.5
12	2143.0
13	1991.5
14	1840.0
15	1703.5
16	1567.0
17	1448.5
18	1330.0
19	1184.0
20	1038.0
21	933.0
22	828.0
23	803.5
24	779.0
25	732.0
26	620.5
27	556.0
28	567.0
29	578.0
30	615.0
31	652.0
32	689.5
33	727.0
34	804.5
35	882.0
36	1044.5
37	1207.0
38	1507.0
39	1807.0
40	2385.0
41	2963.0
42	4031.5
43	5100.0
44	6221.5
45	7343.0
46	10009.5
47	12676.0
48	15718.5
49	18761.0
50	18758.0
51	18755.0
52	14971.0
53	11187.0
54	8213.0
55	5239.0
56	3838.5
57	2438.0
58	2000.0
59	1562.0
60	1377.0
61	1192.0
62	1093.5
63	995.0
64	910.0
65	825.0
66	681.0
67	537.0
68	474.0
69	411.0
70	362.5
71	314.0
72	252.0
73	190.0
74	154.0
75	104.0
76	90.0
77	61.5
78	33.0
79	29.0
80	25.0
81	18.0
82	11.0
83	8.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	130115.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.21788417937978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.81241486968366	38.57356953464243
2	11.228784377343475	11.277715866733274
3	4.715263004851471	7.103715943588364
4	2.4150227269249016	4.851093263651386
5	1.3421894369538268	3.370095684586712
6	0.8708161797340108	2.623832763324751
7	0.5631992164184815	1.9797871114014525
8	0.34128648168837333	1.3710948007531798
9	0.2754778775959964	1.2450524535987395
>10	1.3177025145008494	11.911770356991893
>50	0.07652163266555455	2.627675517811167
>100	0.033669518372844	3.0088767628636206
>500	0.006121730613244365	2.2203435422510855
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0015304326533110912	7.835376397801945
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10195	7.835376397801945	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	941	0.7232063943434653	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	716	0.5502824424547516	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	698	0.5364485263036545	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	534	0.4104061791492142	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	498	0.38273834684701996	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	456	0.3504592091611267	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	243	0.18675786803981093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	195	0.14986742497021865	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	193	0.1483303231756523	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	173	0.13295930522998886	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	172	0.13219075433270566	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	171	0.13142220343542252	No Hit
GTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	166	0.12757944894900666	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	159	0.12219959266802445	TruSeq Adapter, Index 27 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	155	0.11912538907889174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	153	0.11758828728432541	No Hit
GTGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	151	0.11605118548975905	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139	0.10682857472236099	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015371017945663451	0.0	0.0	0.5871728855243439	0.0
2	0.0015371017945663451	0.0	0.0	2.0635591592053184	0.0
3	0.0015371017945663451	0.0	0.0	2.9912000922261077	0.0
4	0.0015371017945663451	0.0	0.0	4.36767474926027	0.0
5	0.0015371017945663451	0.0	0.0	7.106021596280214	0.0
6	0.0015371017945663451	0.0	0.0	8.93901548630058	0.0
7	0.0015371017945663451	0.0	0.0	10.642124274680091	0.0
8	0.0015371017945663451	0.0	0.0	13.336663720554894	0.0
9	0.0015371017945663451	0.0	0.0	14.635514736963456	0.0
10	0.0015371017945663451	0.0	0.0	17.81270414633209	0.0
11	0.0015371017945663451	0.0	0.0	25.6403950351612	0.0
12	0.0015371017945663451	0.0	0.0	32.79252968527841	0.0
13	0.0015371017945663451	0.0	0.0	35.09049686815509	0.0
14	0.0015371017945663451	0.0	0.0	35.989701417976406	0.0
15	0.0015371017945663451	0.0	0.0	37.161741536333246	0.0
16	0.0015371017945663451	0.0	0.0	39.91469085040157	0.0
17	0.0015371017945663451	0.0	0.0	43.37701264266226	0.0
18	0.0015371017945663451	0.0	0.0	46.49348653114553	0.0
19	0.0015371017945663451	0.0	0.0	48.23655996618376	0.0
20	0.0015371017945663451	0.0	0.0	49.696038120124506	0.0
21	0.0015371017945663451	0.0	0.0	51.23313991469085	0.0
22	0.0015371017945663451	0.0	0.0	52.649579218383735	0.0
23	0.0015371017945663451	0.0	0.0	53.70403104945625	0.0
24	0.0015371017945663451	0.0	0.0	54.22357145601968	0.0
25	0.0015371017945663451	0.0	0.0	54.56557660531069	0.0
26	0.0015371017945663451	0.0	0.0	54.79921607808477	0.0
27	0.0015371017945663451	0.0	0.0	55.022864389194176	0.0
28	0.0015371017945663451	0.0	0.0	55.22960458056335	0.0
29	0.0015371017945663451	0.0	0.0	55.39791722706836	0.0
30	0.0015371017945663451	0.0	0.0	55.574683933443495	0.0
31	0.0015371017945663451	0.0	0.0	55.72685701110556	0.0
32	0.0015371017945663451	0.0	0.0	55.83983399300619	0.0
33	0.0015371017945663451	0.0	0.0	55.975867501825306	0.0
34	0.0015371017945663451	0.0	0.0	56.10037274718518	0.0
35	0.0015371017945663451	0.0	0.0	56.24024901049072	0.0
36	0.0015371017945663451	0.0	0.0	56.34554048341852	0.0
37	0.0015371017945663451	0.0	0.0	56.47926833954579	0.0
38	0.0015371017945663451	0.0	0.0	56.596088075932826	0.0
39	0.0015371017945663451	0.0	0.0	56.709065057833456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCT	20	7.010218E-4	45.0	9
CTGGGTA	20	7.010218E-4	45.0	5
CGGGGTG	20	7.010218E-4	45.0	6
CTCCTGG	55	1.8189894E-12	45.0	2
ATGATCT	25	3.8716968E-5	45.0	11
GGACCGA	25	3.8716968E-5	45.0	8
CGCTTGG	40	6.7357178E-9	45.0	2
TGCTGCG	20	7.010218E-4	45.0	1
GCGCGGG	20	7.010218E-4	45.0	3
TAGTCTG	20	7.010218E-4	45.0	1
CGGGCTC	25	3.8716968E-5	45.0	6
GTTTATG	20	7.010218E-4	45.0	1
CGGGATC	20	7.010218E-4	45.0	6
GTCTTTG	35	1.2008604E-7	45.0	1
GACCGAT	25	3.8716968E-5	45.0	9
ATGCTAC	20	7.010218E-4	45.0	11
TAGGGTC	20	7.010218E-4	45.0	5
TAGGGAC	45	3.783498E-10	45.0	5
TGTATGG	20	7.010218E-4	45.0	2
GGTATGC	25	3.8716968E-5	45.0	8
>>END_MODULE