SRR2933637.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129506
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9977	7.703890167250939	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	901	0.6957206615909688	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	734	0.5667691072228314	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	727	0.5613639522493166	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	520	0.40152579803252364	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	494	0.3814495081308974	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	483	0.3729556931725171	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	240	0.18531959909193396	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	181	0.13976186431516685	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	181	0.13976186431516685	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	175	0.13512887433786852	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	160	0.12354639939462263	TruSeq Adapter, Index 27 (95% over 21bp)
GTGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	158	0.12200206940218986	No Hit
GTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	153	0.1181412444211079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.11736907942489151	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	150	0.11582474943245871	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144	0.11119175945516037	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	134	0.10347010949299645	No Hit
GTTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	133	0.10269794449678007	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	132	0.10192577950056368	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	130	0.10038144950813091	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCTA	20	7.0100965E-4	45.000004	13
TTGTTAG	20	7.0100965E-4	45.000004	1
GGCCGAT	20	7.0100965E-4	45.000004	8
TGTTTAG	20	7.0100965E-4	45.000004	1
GGCCTAA	20	7.0100965E-4	45.000004	8
TCTGAGG	20	7.0100965E-4	45.000004	2
GCGTTGG	20	7.0100965E-4	45.000004	2
CGTGTTT	20	7.0100965E-4	45.000004	1
TGAGGGC	20	7.0100965E-4	45.000004	4
GTAGGGC	20	7.0100965E-4	45.000004	4
GAGAGGA	20	7.0100965E-4	45.000004	32
CTTGAGG	20	7.0100965E-4	45.000004	2
CTACTGG	20	7.0100965E-4	45.000004	2
GTGCTTG	40	6.7338988E-9	45.000004	1
GGGCCGA	20	7.0100965E-4	45.000004	7
TAGCGGG	25	3.8715967E-5	45.0	3
GCGGGAA	25	3.8715967E-5	45.0	5
CTGGGTA	35	1.2007877E-7	45.0	5
GCTAGAG	25	3.8715967E-5	45.0	6
AGGGACC	25	3.8715967E-5	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content