##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	129506
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.842509227371703	33.0	31.0	34.0	30.0	34.0
2	32.06016709650518	33.0	31.0	34.0	30.0	34.0
3	32.15332108164873	33.0	31.0	34.0	30.0	34.0
4	35.76181798526709	37.0	35.0	37.0	35.0	37.0
5	35.87385912621809	37.0	35.0	37.0	35.0	37.0
6	35.871882383827774	37.0	35.0	37.0	35.0	37.0
7	35.83286488656896	37.0	35.0	37.0	35.0	37.0
8	35.67393016539774	37.0	35.0	37.0	33.0	37.0
9	37.4875913085108	39.0	37.0	39.0	34.0	39.0
10	37.07058360230414	39.0	37.0	39.0	33.0	39.0
11	37.126194925331646	39.0	37.0	39.0	33.0	39.0
12	37.180972310163234	39.0	37.0	39.0	34.0	39.0
13	37.15003938041481	39.0	37.0	39.0	33.0	39.0
14	38.22726360168641	40.0	38.0	41.0	33.0	41.0
15	38.203921053850785	40.0	37.0	41.0	33.0	41.0
16	38.37381279631832	40.0	38.0	41.0	34.0	41.0
17	38.45492100752089	40.0	38.0	41.0	34.0	41.0
18	38.14386206044507	39.0	38.0	40.0	34.0	41.0
19	37.74361033465631	38.0	37.0	40.0	34.0	41.0
20	37.09500718113446	38.0	35.0	40.0	34.0	41.0
21	36.98681914351459	38.0	35.0	40.0	33.0	41.0
22	36.99164517474094	38.0	35.0	40.0	33.0	41.0
23	36.87687829135329	38.0	35.0	40.0	33.0	41.0
24	36.76133152131948	38.0	35.0	40.0	33.0	41.0
25	36.66525875249023	38.0	35.0	40.0	33.0	41.0
26	36.67644742328541	38.0	35.0	40.0	33.0	41.0
27	36.63220236900221	38.0	35.0	40.0	33.0	41.0
28	36.599107377264374	38.0	35.0	40.0	33.0	41.0
29	36.40936327274412	38.0	35.0	40.0	32.0	41.0
30	36.16190755640665	38.0	35.0	40.0	31.0	41.0
31	35.780458048275754	38.0	35.0	40.0	30.0	41.0
32	35.313390885364385	38.0	35.0	40.0	27.0	41.0
33	34.68674810433493	38.0	35.0	40.0	22.0	41.0
34	34.28072830602443	38.0	35.0	40.0	18.0	41.0
35	33.98935184470218	38.0	34.0	40.0	16.0	41.0
36	33.77854307908514	38.0	34.0	40.0	15.0	41.0
37	33.56808950936636	38.0	33.0	40.0	13.0	41.0
38	33.260296820224546	37.0	33.0	40.0	12.0	41.0
39	33.200129723719364	38.0	33.0	40.0	10.0	41.0
40	33.17158278380924	38.0	33.0	40.0	10.0	41.0
41	33.08713109817306	38.0	33.0	40.0	10.0	41.0
42	32.85258598057233	37.0	33.0	40.0	10.0	41.0
43	32.680841042113876	37.0	32.0	40.0	10.0	41.0
44	32.53464704338023	37.0	31.0	40.0	10.0	41.0
45	32.46761540005868	37.0	31.0	40.0	10.0	41.0
46	32.11648108967924	37.0	31.0	40.0	9.0	41.0
47	32.00123546399394	36.0	31.0	40.0	10.0	41.0
48	31.937354253856963	36.0	30.0	40.0	8.0	41.0
49	32.01509582567603	36.0	31.0	40.0	8.0	41.0
50	31.815282689605116	36.0	30.0	40.0	8.0	41.0
51	30.849219341188824	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	2.0
12	3.0
13	2.0
14	6.0
15	6.0
16	10.0
17	33.0
18	53.0
19	80.0
20	185.0
21	287.0
22	477.0
23	772.0
24	1416.0
25	2803.0
26	4045.0
27	4228.0
28	3904.0
29	3526.0
30	3451.0
31	3758.0
32	4174.0
33	4867.0
34	7023.0
35	10533.0
36	13950.0
37	14787.0
38	24943.0
39	20172.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.551897209395705	4.622951832347536	36.81219402962025	37.01295692863651
2	43.02735008416598	4.71406730190107	35.88791252914923	16.37067008478372
3	17.536639229070467	4.778156996587031	59.770203697126	17.9150000772165
4	13.851095702129632	4.61368585239294	61.177088320232265	20.358130125245165
5	11.494448133677205	5.331027133877967	61.880530631785405	21.29399410065943
6	15.158371040723981	6.9070158911556225	66.14442574089232	11.790187327228082
7	56.54023751795284	2.6160950071811344	35.31342177196423	5.530245702901796
8	54.0700816950566	2.9627970904822942	35.554337250783746	7.412783963677358
9	49.117415409324664	4.985869380569239	37.92025079919077	7.976464410915324
10	24.72626750884129	16.91504640711627	43.089123283863294	15.269562800179143
11	15.297360740042931	14.411687489382732	47.05959569440798	23.231356076166353
12	13.566938983521998	12.36390591941686	54.36890954859235	19.700245548468796
13	17.971368121940294	14.180037990517814	54.80827143143947	13.040322456102421
14	15.403919509520794	16.226275230491254	52.773616666409275	15.596188593578674
15	10.11536145043473	14.834833907309314	55.546461167822336	19.503343474433617
16	8.666779917532779	14.891201952033112	52.25086096397078	24.19115716646333
17	8.763300542059827	14.81012462743039	50.38685466310441	26.039720167405374
18	10.119994440412029	13.899742096891263	54.744953901749724	21.235309560946984
19	10.94543882136735	16.137476255926366	55.63371581239479	17.28336911031149
20	10.798727472086235	20.05003629175482	53.02148163019474	16.1297546059642
21	9.748583077231942	21.605176594134633	52.548916652510314	16.097323676123114
22	8.098466480317516	19.9689589671521	49.732058746312916	22.20051580621747
23	8.763300542059827	20.73340231340633	46.57467607678409	23.928621067749756
24	10.719194477475948	19.58596512902877	46.355381217858636	23.33945917563665
25	9.050545920652326	22.234491066050992	44.69445431099717	24.020508702299505
26	8.27065927447377	22.356493135453185	47.632542121600544	21.740305468472503
27	11.331521319475545	20.763516748258766	48.83557518570568	19.069386746560006
28	9.503034608435131	17.79531450280296	49.22397417880253	23.477676709959383
29	10.789461492131638	16.63938350346702	48.634812286689424	23.93634271771192
30	16.772968047812455	17.989127916853274	45.719889425972546	19.518014609361728
31	17.136657761030378	23.794264358408103	39.87768906459932	19.191388815962195
32	14.178493660525382	24.08845922196655	39.06768798356833	22.66535913393974
33	16.82779176254382	17.70033820826834	39.10706839837537	26.364801630812472
34	17.6748567633932	15.479591679150001	41.3370809074483	25.50847065000849
35	15.43943909934675	16.383796889719395	42.2412861180177	25.935477892916158
36	17.523512424134786	16.110450481058795	41.780303615276516	24.585733479529907
37	20.917949747502046	19.001436226892963	37.22839096258088	22.85222306302411
38	21.584328139236792	23.581918984448595	34.730437199820855	20.103315676493754
39	18.087192871372753	20.134202276342407	42.10615724367983	19.672447608605008
40	18.255524840547928	14.990039071548807	48.65566074158726	18.098775346316
41	20.31643321544948	15.227093725387242	41.46371596682779	22.992757092335488
42	22.726360168640834	14.587741108520069	36.94500640896947	25.74089231386963
43	19.303352740413573	15.31512053495591	39.46921378160086	25.912312943029665
44	17.76056707797322	16.101184501104196	40.00432412397881	26.13392429694377
45	20.82297345296743	14.00707303136534	40.28462001760536	24.885333498061865
46	23.205102466294996	14.412459654378948	44.48751409201118	17.894923787314873
47	16.671042268311894	18.514972279276638	45.633406946396306	19.180578506015163
48	16.17067935076367	17.728136148132133	44.5214893518447	21.579695149259493
49	20.701743548561456	12.661189442960172	45.45658116226276	21.180485846215618
50	20.182848671104043	10.669003752721881	41.594984016184576	27.553163559989496
51	17.477182524361805	10.480595493645083	38.08394977838865	33.958272203604466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	424.0
2	574.0
3	7366.0
4	14158.0
5	8491.5
6	2825.0
7	2627.0
8	2429.0
9	2379.0
10	2329.0
11	2247.0
12	2165.0
13	2009.0
14	1853.0
15	1692.0
16	1531.0
17	1407.0
18	1283.0
19	1176.5
20	1070.0
21	986.0
22	902.0
23	838.5
24	775.0
25	722.5
26	615.5
27	561.0
28	557.0
29	553.0
30	591.0
31	629.0
32	717.0
33	805.0
34	892.5
35	980.0
36	1110.5
37	1241.0
38	1443.5
39	1646.0
40	2324.0
41	3002.0
42	3990.5
43	4979.0
44	6076.0
45	7173.0
46	9881.5
47	12590.0
48	15530.5
49	18471.0
50	18614.5
51	18758.0
52	15007.0
53	11256.0
54	8225.0
55	5194.0
56	3807.0
57	2420.0
58	1979.5
59	1539.0
60	1370.5
61	1202.0
62	1112.5
63	1023.0
64	932.0
65	841.0
66	711.0
67	581.0
68	493.0
69	405.0
70	344.0
71	283.0
72	253.0
73	223.0
74	179.5
75	116.0
76	96.0
77	69.5
78	43.0
79	33.5
80	24.0
81	16.5
82	9.0
83	6.0
84	3.0
85	1.5
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	129506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.69880932157583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16957568003899	39.124056028292124
2	11.08166560053611	11.236545024940929
3	4.665082701270219	7.095424150232422
4	2.433823753388772	4.935678655815175
5	1.2565110116055926	3.185180609392615
6	0.8041670474275793	2.4462187080135283
7	0.5635261506594779	1.999907340200454
8	0.354869170550428	1.4393155529473538
9	0.25587133327241157	1.167513474279184
>10	1.3022023211185234	11.918366716600003
>50	0.07310609522068902	2.626905317128164
>100	0.031983916659051446	2.895618735811468
>500	0.006092174601724086	2.2253795190956405
>1k	0.0	0.0
>5k	0.0015230436504310215	7.703890167250939
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9977	7.703890167250939	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	901	0.6957206615909688	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	734	0.5667691072228314	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	727	0.5613639522493166	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	520	0.40152579803252364	TruSeq Adapter, Index 27 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	494	0.3814495081308974	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	483	0.3729556931725171	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	240	0.18531959909193396	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	181	0.13976186431516685	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	181	0.13976186431516685	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	175	0.13512887433786852	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	160	0.12354639939462263	TruSeq Adapter, Index 27 (95% over 21bp)
GTGCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	158	0.12200206940218986	No Hit
GTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	153	0.1181412444211079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152	0.11736907942489151	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	150	0.11582474943245871	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144	0.11119175945516037	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	134	0.10347010949299645	No Hit
GTTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	133	0.10269794449678007	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	132	0.10192577950056368	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	130	0.10038144950813091	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5937948820904051	0.0
2	0.0	0.0	0.0	2.0778960048183097	0.0
3	0.0	0.0	0.0	3.0284311151606875	0.0
4	0.0	0.0	0.0	4.360415733633963	0.0
5	0.0	0.0	0.0	7.128627245069726	0.0
6	0.0	0.0	0.0	8.9787345760042	0.0
7	0.0	0.0	0.0	10.730004787422976	0.0
8	0.0	0.0	0.0	13.427177119206831	0.0
9	0.0	0.0	0.0	14.723642147854154	0.0
10	0.0	0.0	0.0	17.976001111917594	0.0
11	0.0	0.0	0.0	25.629700554414466	0.0
12	0.0	0.0	0.0	32.60235047024848	0.0
13	0.0	0.0	0.0	34.87251555912467	0.0
14	0.0	0.0	0.0	35.69795994008	0.0
15	0.0	0.0	0.0	36.8569795994008	0.0
16	0.0	0.0	0.0	39.5325313112906	0.0
17	0.0	0.0	0.0	43.04047688910166	0.0
18	0.0	0.0	0.0	46.040337899402346	0.0
19	0.0	0.0	0.0	47.73678439608975	0.0
20	0.0	0.0	0.0	49.161428814109	0.0
21	0.0	0.0	0.0	50.70807530153043	0.0
22	0.0	0.0	0.0	52.07326301484101	0.0
23	0.0	0.0	0.0	53.08788781986935	0.0
24	0.0	0.0	0.0	53.57049094250459	0.0
25	0.0	0.0	0.0	53.914876530817104	0.0
26	0.0	0.0	0.0	54.17509613454203	0.0
27	0.0	0.0	0.0	54.40056831343721	0.0
28	0.0	0.0	0.0	54.61368585239294	0.0
29	0.0	0.0	0.0	54.78742297654163	0.0
30	0.0	0.0	0.0	54.968109585656265	0.0
31	0.0	0.0	0.0	55.114048769941164	0.0
32	0.0	0.0	0.0	55.248405479282816	0.0
33	0.0	0.0	0.0	55.38585084860933	0.0
34	0.0	0.0	0.0	55.52870137290936	0.0
35	0.0	0.0	0.0	55.654564267292635	0.0
36	0.0	0.0	0.0	55.76730035674023	0.0
37	0.0	0.0	0.0	55.88466943616512	0.0
38	0.0	0.0	0.0	56.00976016555217	0.0
39	0.0	0.0	0.0	56.150294194863555	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCTA	20	7.0100965E-4	45.000004	13
TTGTTAG	20	7.0100965E-4	45.000004	1
GGCCGAT	20	7.0100965E-4	45.000004	8
TGTTTAG	20	7.0100965E-4	45.000004	1
GGCCTAA	20	7.0100965E-4	45.000004	8
TCTGAGG	20	7.0100965E-4	45.000004	2
GCGTTGG	20	7.0100965E-4	45.000004	2
CGTGTTT	20	7.0100965E-4	45.000004	1
TGAGGGC	20	7.0100965E-4	45.000004	4
GTAGGGC	20	7.0100965E-4	45.000004	4
GAGAGGA	20	7.0100965E-4	45.000004	32
CTTGAGG	20	7.0100965E-4	45.000004	2
CTACTGG	20	7.0100965E-4	45.000004	2
GTGCTTG	40	6.7338988E-9	45.000004	1
GGGCCGA	20	7.0100965E-4	45.000004	7
TAGCGGG	25	3.8715967E-5	45.0	3
GCGGGAA	25	3.8715967E-5	45.0	5
CTGGGTA	35	1.2007877E-7	45.0	5
GCTAGAG	25	3.8715967E-5	45.0	6
AGGGACC	25	3.8715967E-5	45.0	6
>>END_MODULE