##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172143
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96218841312165	33.0	31.0	34.0	30.0	34.0
2	32.14401979749395	33.0	31.0	34.0	30.0	34.0
3	32.280179850473154	34.0	31.0	34.0	30.0	34.0
4	35.805295597265065	37.0	35.0	37.0	35.0	37.0
5	35.8804075681265	37.0	35.0	37.0	35.0	37.0
6	35.859564431896736	37.0	35.0	37.0	35.0	37.0
7	35.813271524255995	37.0	35.0	37.0	35.0	37.0
8	35.646270832970266	37.0	35.0	37.0	33.0	37.0
9	37.503163067914464	39.0	37.0	39.0	35.0	39.0
10	37.06043812411774	39.0	37.0	39.0	33.0	39.0
11	37.046101206555015	39.0	37.0	39.0	33.0	39.0
12	37.05320576497447	39.0	37.0	39.0	33.0	39.0
13	37.03930453169748	39.0	37.0	39.0	33.0	39.0
14	38.12956088833121	40.0	37.0	41.0	33.0	41.0
15	38.1459948995893	40.0	37.0	41.0	33.0	41.0
16	38.29407527462633	40.0	38.0	41.0	34.0	41.0
17	38.281800596016105	40.0	38.0	41.0	34.0	41.0
18	38.15309945800875	39.0	37.0	41.0	34.0	41.0
19	37.94487141504447	39.0	37.0	41.0	34.0	41.0
20	37.54708004391698	39.0	35.0	41.0	34.0	41.0
21	37.45843281457858	39.0	35.0	41.0	33.0	41.0
22	37.46923778486491	39.0	35.0	41.0	33.0	41.0
23	37.3689142166687	39.0	35.0	40.0	33.0	41.0
24	37.30327692674114	39.0	35.0	41.0	33.0	41.0
25	37.20883800096431	39.0	35.0	41.0	33.0	41.0
26	37.16323637905695	39.0	35.0	40.0	33.0	41.0
27	37.052671325583965	39.0	35.0	40.0	33.0	41.0
28	37.00856845761954	39.0	35.0	40.0	33.0	41.0
29	36.89796854940369	39.0	35.0	40.0	33.0	41.0
30	36.64183847150334	39.0	35.0	40.0	31.0	41.0
31	36.33910179327652	38.0	35.0	40.0	31.0	41.0
32	36.11808786880675	38.0	35.0	40.0	30.0	41.0
33	35.6487106649704	38.0	35.0	40.0	27.0	41.0
34	35.35070842264861	38.0	35.0	40.0	25.0	41.0
35	35.14074345166519	38.0	35.0	40.0	23.0	41.0
36	34.86045322783964	38.0	35.0	40.0	22.0	41.0
37	34.71615459240283	38.0	34.0	40.0	21.0	41.0
38	34.6463521607036	38.0	34.0	40.0	21.0	41.0
39	34.63643017723637	38.0	34.0	40.0	21.0	41.0
40	34.44027930267278	38.0	34.0	40.0	21.0	41.0
41	34.32411425384709	38.0	34.0	40.0	20.0	41.0
42	34.14756336301796	38.0	33.0	40.0	18.0	41.0
43	34.02413690942995	38.0	33.0	40.0	18.0	41.0
44	33.89218266208908	38.0	33.0	40.0	18.0	41.0
45	33.77243338387271	37.0	33.0	40.0	18.0	41.0
46	33.400905061489574	37.0	32.0	40.0	17.0	41.0
47	33.248996473861844	37.0	32.0	40.0	17.0	41.0
48	33.20356912566878	36.0	32.0	40.0	17.0	41.0
49	33.25299315104303	36.0	32.0	40.0	17.0	41.0
50	33.071399940746936	36.0	32.0	40.0	15.0	41.0
51	32.07487960590904	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	1.0
11	10.0
12	7.0
13	8.0
14	11.0
15	6.0
16	17.0
17	46.0
18	56.0
19	99.0
20	205.0
21	323.0
22	546.0
23	885.0
24	1378.0
25	2349.0
26	3498.0
27	4088.0
28	4107.0
29	3961.0
30	4079.0
31	4580.0
32	5489.0
33	6861.0
34	10053.0
35	13189.0
36	16712.0
37	21632.0
38	35198.0
39	32726.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.7872815043307	5.784725489854365	39.96619089942664	28.461802106388294
2	34.107689537187106	5.436758973644006	41.83847150334315	18.61707998582574
3	20.694422660230156	5.825389356523355	54.07771445832825	19.402473524918236
4	16.079073793299756	5.794020087950134	54.86717438408765	23.259731734662463
5	14.354925846534567	6.708957088002417	55.67987080508647	23.25624626037655
6	18.506706633438476	7.9526904956925355	59.260614721481566	14.27998814938743
7	68.07596010293767	3.12647043446437	23.68147412325799	5.116095339339967
8	66.69164589904905	3.7910341983118685	23.261474471805418	6.255845430833667
9	61.15555090825651	6.215181564164677	25.82039351004688	6.808874017531935
10	28.417071853052406	24.60686754616801	31.533085864659032	15.44297473612055
11	21.024380892629964	19.78936117065463	37.8133296154941	21.37292832122131
12	19.33392586396194	17.278657860034972	42.98170706912276	20.405709206880328
13	21.359567336458642	18.37483952295475	44.55133232254579	15.714260818040815
14	18.620565460111653	20.268032972586745	43.23091848056558	17.880483086736028
15	14.927124541805359	19.634838477312467	44.904527050185024	20.533509930697154
16	15.523140644696559	19.59766008492939	42.463533225283626	22.41566604509042
17	14.971854795141248	19.689444241125113	41.3812934595075	23.95740750422614
18	16.72446744857473	17.90952870578531	43.32270263676129	22.043301208878667
19	16.558907419993844	21.382803831698066	43.25241223866204	18.80587650964605
20	17.88919677245081	23.53450329086864	40.83930220804796	17.736997728632588
21	16.86795280667817	24.84852709665801	40.818970274713465	17.464549821950357
22	14.768535461796297	22.35525115746792	40.0887634118146	22.787449968921187
23	14.468203760826754	24.200809791859093	38.18104715265797	23.14993929465619
24	15.303555764684013	23.00354937464782	38.71083924411681	22.982055616551357
25	15.669530564704925	25.27491678430142	35.67150566680027	23.384046984193375
26	15.121730189435528	25.097738508100825	37.66171148405686	22.11881981840679
27	16.00355518377163	24.97632782047484	38.95540335651174	20.064713639241795
28	14.077830640804448	24.296660334721714	38.58013395839506	23.045375066078783
29	17.42853325432925	22.59574888319595	37.02270786497272	22.95300999750208
30	21.123716909778498	24.86595446808758	35.708684059183355	18.30164456295057
31	20.426622052595807	30.007029039809925	30.77209064556793	18.79425826202634
32	20.33716154592403	28.77781844164445	29.670680771219278	21.214339241212247
33	20.84371714214345	26.90205236344202	28.633752171160026	23.620478323254503
34	21.088862166919363	26.434998809129617	29.883294702659995	22.592844321291018
35	23.869689734697317	26.189853784353705	27.894831622546373	22.045624858402608
36	22.620728115578327	32.68038781710555	26.419314174843006	18.27956989247312
37	20.39583369640357	34.58519951435725	26.78935536153082	18.22961142770836
38	23.409026216575754	30.96727720557908	26.629604456759786	18.994092121085377
39	23.826121306123397	28.04180245493572	26.789936273911806	21.342139965029077
40	23.806370285169887	29.80951883027483	28.11674015208286	18.267370732472422
41	22.783383582254288	29.323876079770887	26.473339026274665	21.419401311700156
42	24.239150009004142	26.257239620548035	27.44171996537762	22.0618904050702
43	21.300314273598115	24.507531529019477	31.38379138274574	22.808362814636666
44	21.221310189784077	25.40852663192811	30.21790023410769	23.15226294418013
45	23.939399220415584	23.98877677279936	28.29798481495036	23.773839191834696
46	24.44653572901599	25.671679940514576	30.573999523651846	19.307784806817587
47	19.421062721109774	28.177735952086348	33.141051335227104	19.260149991576768
48	19.012681317276915	26.31010264721772	33.748104773357035	20.929111262148332
49	22.427865205091116	22.24081141841376	34.39756481529891	20.933758561196214
50	21.373509233602295	20.67176707737172	32.43466187994864	25.520061809077337
51	19.09865634966278	19.834672336371504	30.568771312222975	30.49790000174274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	477.0
1	914.5
2	1352.0
3	5443.0
4	9534.0
5	5979.0
6	2424.0
7	2280.5
8	2137.0
9	2115.5
10	2094.0
11	2010.5
12	1927.0
13	1812.5
14	1698.0
15	1581.0
16	1464.0
17	1354.0
18	1244.0
19	1214.5
20	1185.0
21	1119.5
22	1054.0
23	1021.0
24	988.0
25	1058.5
26	1225.5
27	1322.0
28	1517.0
29	1712.0
30	1797.5
31	1883.0
32	2084.0
33	2285.0
34	2646.0
35	3007.0
36	3265.0
37	3523.0
38	3772.5
39	4022.0
40	4744.0
41	5466.0
42	6501.0
43	7536.0
44	8644.5
45	9753.0
46	11940.0
47	14127.0
48	17215.0
49	20303.0
50	20427.0
51	20551.0
52	17814.5
53	15078.0
54	11909.0
55	8740.0
56	7318.5
57	5897.0
58	5172.0
59	4447.0
60	4136.0
61	3825.0
62	3408.5
63	2992.0
64	2607.0
65	2222.0
66	1894.5
67	1567.0
68	1317.0
69	1067.0
70	875.0
71	683.0
72	630.0
73	577.0
74	483.0
75	318.0
76	247.0
77	186.5
78	126.0
79	92.0
80	58.0
81	36.5
82	15.0
83	11.0
84	7.0
85	7.5
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	172143.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.31920554422777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06084867894315	43.597474192967475
2	11.984270961072657	13.738577810308872
3	5.197069047643177	8.9367560690821
4	2.666436947025975	6.113521897492201
5	1.3773043751456862	3.947299628796989
6	0.7702364423184117	2.6489604572942262
7	0.46518227240019866	1.8664714801066555
8	0.30100029390601085	1.3802478172217283
9	0.2280305256863719	1.1763475714957914
>10	0.8847584396631228	8.595179589062582
>50	0.04256569812812275	1.6393347391412953
>100	0.01824244205490975	1.9977576782093955
>500	0.0030404070091516254	1.0839825029190846
>1k	0.0	0.0
>5k	0.0010134690030505417	3.2780885659016055
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5643	3.2780885659016055	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	713	0.4141905276427157	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	595	0.3456428666864177	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	558	0.3241491085899514	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	394	0.22887947810831694	No Hit
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	378	0.2195848800125477	RNA PCR Primer, Index 40 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	364	0.21145210667874964	RNA PCR Primer, Index 40 (95% over 24bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.19867203429706695	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	205	0.11908703810204306	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.11676338857810077	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3956013314511772	0.0
2	0.0	0.0	0.0	1.2524470934049017	0.0
3	0.0	0.0	0.0	1.7380898439088432	0.0
4	0.0	0.0	0.0	2.516512434429515	0.0
5	0.0	0.0	0.0	4.223233009765137	0.0
6	0.0	0.0	0.0	5.766136293662827	0.0
7	0.0	0.0	0.0	7.10107294516768	0.0
8	0.0	0.0	0.0	9.19816664052561	0.0
9	0.0	0.0	0.0	10.156091156770824	0.0
10	0.0	0.0	0.0	12.293848718797744	0.0
11	0.0	0.0	0.0	17.233346694318097	0.0
12	0.0	0.0	0.0	21.878903005059747	0.0
13	0.0	0.0	0.0	23.332926694666643	0.0
14	0.0	0.0	0.0	23.872594296602244	0.0
15	0.0	0.0	0.0	24.657406923313758	0.0
16	0.0	0.0	0.0	26.487861835799308	0.0
17	0.0	0.0	0.0	28.586117355919207	0.0
18	0.0	0.0	0.0	30.567609487461006	0.0
19	0.0	0.0	0.0	31.823542055151822	0.0
20	0.0	0.0	0.0	32.94121747616807	0.0
21	0.0	0.0	0.0	34.18204632195326	0.0
22	0.0	0.0	0.0	35.314825464875135	0.0
23	0.0	0.0	0.0	36.154824767780276	0.0
24	0.0	0.0	0.0	36.6369820439983	0.0
25	0.0	0.0	0.0	36.96055024020727	0.0
26	0.0	0.0	0.0	37.184782419267705	0.0
27	0.0	0.0	0.0	37.399720000232364	0.0
28	0.0	0.0	0.0	37.60129659643436	0.0
29	0.0	0.0	0.0	37.833080636447605	0.0
30	0.0	0.0	0.0	38.06835015074676	0.0
31	0.0	0.0	0.0	38.26469853551989	0.0
32	0.0	0.0	0.0	38.51565268410565	0.0
33	0.0	0.0	0.0	38.728266615546374	0.0
34	0.0	0.0	0.0	38.90834945365191	0.0
35	0.0	0.0	0.0	39.07797586889969	0.0
36	0.0	0.0	0.0	39.25980144414818	0.0
37	0.0	0.0	0.0	39.43058968415794	0.0
38	0.0	0.0	0.0	39.63448992988388	0.0
39	0.0	0.0	0.0	39.94527805371116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATA	20	7.0165604E-4	45.000004	19
GTGCAGG	20	7.0165604E-4	45.000004	2
CCGGGAT	25	3.8769474E-5	45.000004	5
ATAGGTG	25	3.8769474E-5	45.000004	24
TTGCTAG	20	7.0165604E-4	45.000004	1
TAGGTGG	25	3.8769474E-5	45.000004	25
ACCGATC	25	3.8769474E-5	45.000004	10
TTGTACG	20	7.0165604E-4	45.000004	1
TAGCCTT	20	7.0165604E-4	45.000004	38
GCCGATT	25	3.8769474E-5	45.000004	9
CGTAGGG	25	3.8769474E-5	45.000004	3
CGGGCTA	20	7.0165604E-4	45.000004	6
CGGGATT	20	7.0165604E-4	45.000004	6
CAACTAG	20	7.0165604E-4	45.000004	16
AACTAGA	20	7.0165604E-4	45.000004	17
CCCGGGA	25	3.8769474E-5	45.000004	4
GTTATGG	20	7.0165604E-4	45.000004	2
GACCGAT	55	1.8189894E-12	45.0	9
TTTTCCG	35	1.2038981E-7	45.0	1
TCGTTTG	30	2.1547203E-6	44.999996	1
>>END_MODULE