FastQCFastQC Report
Sat 14 Jan 2017
SRR2933634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330510
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96522.9203352394783817No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC4330.13100965175032525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCAC253.884756E-545.08
TACGGGT207.0259866E-445.04
CGCGACC406.7902874E-945.010
ATACGGG253.884756E-545.025
ACGGTCC207.0259866E-445.010
CGTTTTT65200.044.137271
GGCACCG900.042.58
GCACCGA900.042.59
ACACGCG700.041.78571736
CGGGAAC650.041.538466
TTACGGG650.041.538463
CTTTGCG1250.041.41
TGTTTCG556.002665E-1140.9090921
CGGGTAC556.002665E-1140.9090926
ATTGCGG501.0768417E-940.52
GCGCGAC451.9212166E-840.09
ACCGATG403.4482582E-739.37511
TGTTCGG1200.039.3749962
GTTTTTT76750.038.9902272
TGGGCGA4450.038.9325836
Produced by FastQC (version 0.11.5)