##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330510
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.508722882817466	33.0	31.0	34.0	31.0	34.0
2	32.68072070436598	34.0	31.0	34.0	31.0	34.0
3	32.75858219115912	34.0	31.0	34.0	31.0	34.0
4	36.207542888263596	37.0	35.0	37.0	35.0	37.0
5	36.21389973071919	37.0	35.0	37.0	35.0	37.0
6	36.18816677256361	37.0	37.0	37.0	35.0	37.0
7	36.27479350095307	37.0	37.0	37.0	35.0	37.0
8	36.183225923572664	37.0	37.0	37.0	35.0	37.0
9	38.030465038879306	39.0	39.0	39.0	35.0	39.0
10	37.35834619224834	39.0	37.0	39.0	34.0	39.0
11	37.304847054552056	39.0	37.0	39.0	34.0	39.0
12	37.34870654443133	39.0	37.0	39.0	34.0	39.0
13	37.41160933103386	39.0	37.0	39.0	35.0	39.0
14	38.767489637227314	40.0	38.0	41.0	35.0	41.0
15	38.82328825149012	40.0	38.0	41.0	35.0	41.0
16	38.85047351063508	40.0	38.0	41.0	35.0	41.0
17	38.7813349066594	40.0	38.0	41.0	35.0	41.0
18	38.41707663913346	39.0	38.0	41.0	35.0	41.0
19	38.046785271247465	39.0	37.0	41.0	34.0	41.0
20	37.60235091222656	39.0	35.0	41.0	34.0	41.0
21	37.5162778735893	39.0	35.0	41.0	34.0	41.0
22	37.50876221596926	39.0	35.0	41.0	34.0	41.0
23	37.4159269008502	39.0	35.0	41.0	34.0	41.0
24	37.32203261625972	39.0	35.0	41.0	33.0	41.0
25	37.24428912892197	39.0	35.0	41.0	33.0	41.0
26	37.18667211279538	39.0	35.0	41.0	33.0	41.0
27	37.084060996641554	39.0	35.0	41.0	33.0	41.0
28	36.91660766693897	38.0	35.0	41.0	33.0	41.0
29	36.73398081752443	38.0	35.0	41.0	33.0	41.0
30	36.49136788599437	38.0	35.0	41.0	32.0	41.0
31	36.09641160630541	38.0	35.0	41.0	30.0	41.0
32	35.48162234123022	38.0	35.0	41.0	25.0	41.0
33	34.78979455992254	39.0	35.0	41.0	20.0	41.0
34	34.192674956884815	39.0	34.0	41.0	15.0	41.0
35	33.754570209675954	38.0	34.0	41.0	12.0	41.0
36	33.50356116305104	38.0	33.0	41.0	10.0	41.0
37	33.369843575080935	38.0	33.0	41.0	10.0	41.0
38	33.292281625366854	38.0	33.0	41.0	10.0	41.0
39	33.23428338023055	38.0	33.0	41.0	10.0	41.0
40	33.1118090224199	38.0	33.0	41.0	10.0	41.0
41	32.931490726453056	38.0	32.0	40.0	10.0	41.0
42	32.793531209343136	38.0	32.0	40.0	10.0	41.0
43	32.70273214123627	38.0	32.0	40.0	8.0	41.0
44	32.53818341351245	38.0	31.0	40.0	8.0	41.0
45	32.55394390487429	38.0	31.0	40.0	8.0	41.0
46	32.407588272669514	38.0	31.0	40.0	8.0	41.0
47	32.30177301745787	37.0	31.0	40.0	8.0	41.0
48	32.219454782003574	37.0	31.0	40.0	8.0	41.0
49	32.20024810141902	37.0	31.0	40.0	8.0	41.0
50	32.11944267949533	37.0	31.0	40.0	8.0	41.0
51	31.273725454600466	36.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	6.0
14	7.0
15	13.0
16	34.0
17	53.0
18	117.0
19	243.0
20	402.0
21	664.0
22	1186.0
23	2157.0
24	4268.0
25	8344.0
26	12552.0
27	12597.0
28	10423.0
29	8009.0
30	7178.0
31	6898.0
32	7679.0
33	9564.0
34	15050.0
35	19326.0
36	24370.0
37	33784.0
38	62128.0
39	83420.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.787540467761943	4.4458564037396755	37.67177997640011	36.09482315209827
2	37.23729993041058	4.416205258539832	44.95930531602675	13.387189495022845
3	18.58703216241566	4.278539227254848	61.801155789537376	15.33327282079211
4	14.27521103748752	4.294575050679253	63.12365737799159	18.30655653384164
5	13.571752745756557	5.355359898338931	63.02895525097576	18.043932104928746
6	16.77589180357629	6.245801942452573	64.71574233759947	12.262563916371667
7	69.31741853499138	1.9076578620919185	26.128105049771566	2.6468185531451396
8	71.04051314634958	2.0934313636501165	24.582614746906295	2.2834407430940065
9	66.21373029560377	4.680342500983329	25.898157393119725	3.207769810293183
10	29.150706483918793	22.316420078061178	34.950228434843126	13.582645003176907
11	21.514326344134822	22.388127439411818	39.42210523130919	16.67544098514417
12	17.253335753834982	20.205742640162175	45.14356600405434	17.3973556019485
13	18.107772835920244	20.722519742216576	46.20223291277117	14.96747450909201
14	17.894768690811173	21.34942966929896	45.14386856676046	15.611933073129405
15	16.595564430728267	21.794801972708843	45.888777949229976	15.72085564733291
16	17.376478775226165	20.422377537744698	45.05249462951197	17.148649057517172
17	17.277238207618527	20.76971952437143	43.913951166379235	18.039091101630813
18	18.115942028985508	19.582766028259357	45.008623037124444	17.29266890563069
19	18.700795739917098	21.76091494962331	43.24074914526035	16.297540165199237
20	20.084112432301595	21.787843030468064	42.53880366706	15.589240870170343
21	19.653263138785512	22.566941998729238	42.169072040180325	15.610722822304924
22	18.953435599528	20.804514235575322	42.34758403679162	17.894466128105048
23	17.052736679676865	23.026837312032917	41.90372454691234	18.01670146137787
24	16.855465795286072	22.26074854013494	42.7623975068833	18.121388157695684
25	16.85062479198814	23.979909836313578	41.56303893982028	17.606426431878006
26	17.244863998063597	24.11364255241899	41.409942210523134	17.23155123899428
27	17.04789567637893	23.5085171401773	42.27375873649814	17.16982844694563
28	16.854558107167712	22.935765937490547	42.387522313999575	17.82215364134217
29	17.34107893861003	22.103718495658224	41.8740734017125	18.681129164019243
30	18.25360806027049	23.412907325043115	40.90254455235848	17.430940062327917
31	18.66206771353363	25.034038304438592	38.53589906508124	17.767994916946535
32	18.38885358990651	25.184411969380655	37.864512420199084	18.56222202051375
33	19.73071919155245	24.968684759916492	35.54900003025627	19.751596018274785
34	19.820882877976462	25.57381017215818	34.711506459713775	19.893800490151584
35	19.52618680221476	27.104474902423526	33.55269129527094	19.81664700009077
36	20.376388006414327	28.225772291307372	32.34032253184472	19.05751717043357
37	20.90314967777072	28.59762185712989	32.428368279325895	18.0708601857735
38	20.866842153036217	27.41308886266679	32.28313818038789	19.43693080390911
39	21.773622583280385	26.201022661946688	31.953949956128408	20.07140479864452
40	22.424132401440197	26.577108105654894	32.16090284711506	18.83785664578984
41	20.65232519439654	27.337750748842698	32.81292547880548	19.19699857795528
42	20.08986112371789	27.65846721733079	33.28764636470908	18.96402529424223
43	20.066261232640464	26.982239569150707	33.46706604943875	19.484433148770083
44	20.295603763880063	27.286617651508276	32.12005688178875	20.29772170282291
45	21.776043084929352	26.581343983540588	30.941272578741945	20.701340352788115
46	21.61477716256694	26.70993313364195	31.705848537109315	19.969441166681793
47	19.89501074097607	27.63607757707785	33.21351850170948	19.255393180236606
48	20.124050709509547	26.329914374754164	33.866146258812144	19.679888656924145
49	20.53099754924208	25.85368067531996	33.79988502617167	19.815436749266286
50	20.288947384345406	25.851562736377115	32.691295270944906	21.168194608332577
51	19.749478079331944	25.237965568364046	32.51096789809688	22.501588454207134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	395.0
1	757.5
2	1120.0
3	9044.0
4	16968.0
5	11365.5
6	5763.0
7	5612.0
8	5461.0
9	5565.0
10	5669.0
11	5645.5
12	5622.0
13	5536.5
14	5451.0
15	5157.5
16	4864.0
17	4738.0
18	4612.0
19	4498.0
20	4384.0
21	4197.5
22	4011.0
23	4017.5
24	4024.0
25	3974.5
26	4196.5
27	4468.0
28	4816.5
29	5165.0
30	5586.5
31	6008.0
32	6565.5
33	7123.0
34	8077.5
35	9032.0
36	9707.5
37	10383.0
38	11478.5
39	12574.0
40	13719.5
41	14865.0
42	15996.0
43	17127.0
44	18221.5
45	19316.0
46	20809.0
47	22302.0
48	23746.0
49	25190.0
50	24686.0
51	24182.0
52	21919.0
53	19656.0
54	17437.0
55	15218.0
56	13664.5
57	12111.0
58	11099.0
59	10087.0
60	8806.0
61	7525.0
62	6619.5
63	5714.0
64	4791.0
65	3868.0
66	3121.0
67	2374.0
68	1925.5
69	1477.0
70	1216.5
71	956.0
72	774.0
73	592.0
74	521.0
75	335.5
76	221.0
77	174.0
78	127.0
79	105.5
80	84.0
81	55.0
82	26.0
83	18.5
84	11.0
85	7.0
86	3.0
87	3.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	330510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.68486480280596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74435326147596	35.902338685053195
2	9.436406343916103	9.188203341557918
3	4.918642202036966	7.183902918586364
4	3.0438876393306677	5.92765032782983
5	2.1658058794034893	5.272098321394057
6	1.6259215253778627	4.749466178579597
7	1.1430454047824363	3.8954307696711066
8	0.9082611547301657	3.5374857218942823
9	0.6458537382299396	2.8298971735300293
>10	2.332953102566494	16.726819150194878
>50	0.019302896297278736	0.6275164244608676
>100	0.014944177778538376	1.2332061906796181
>500	0.0	0.0
>1k	0.0	0.0
>5k	6.226740741057656E-4	2.925984796568258
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9652	2.9203352394783817	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC	433	0.13100965175032525	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.025627061208435E-4	0.0	0.0	0.06293304287313546	0.0
2	3.025627061208435E-4	0.0	0.0	0.21996308734985326	0.0
3	3.025627061208435E-4	0.0	0.0	0.3367522919124989	0.0
4	3.025627061208435E-4	0.0	0.0	0.5364436779522556	0.0
5	3.025627061208435E-4	0.0	0.0	0.980303167831533	0.0
6	3.025627061208435E-4	0.0	0.0	1.55305437051829	0.0
7	3.025627061208435E-4	0.0	0.0	2.0277752564218936	0.0
8	3.025627061208435E-4	0.0	0.0	2.656803122447127	0.0
9	3.025627061208435E-4	0.0	0.0	2.9990015430698014	0.0
10	3.025627061208435E-4	0.0	0.0	3.6610087440622068	0.0
11	3.025627061208435E-4	0.0	0.0	4.986535959577623	0.0
12	3.025627061208435E-4	0.0	0.0	6.195273970530392	0.0
13	3.025627061208435E-4	0.0	0.0	6.617954070981211	0.0
14	3.025627061208435E-4	0.0	0.0	6.7786148679313785	0.0
15	3.025627061208435E-4	0.0	0.0	6.980121630207861	0.0
16	3.025627061208435E-4	0.0	0.0	7.45332970258086	0.0
17	6.05125412241687E-4	0.0	0.0	8.089619073552994	0.0
18	6.05125412241687E-4	0.0	0.0	8.747692959365828	0.0
19	6.05125412241687E-4	0.0	0.0	9.15010135850655	0.0
20	6.05125412241687E-4	0.0	0.0	9.505309975492422	0.0
21	6.05125412241687E-4	0.0	0.0	9.927384950530998	0.0
22	6.05125412241687E-4	0.0	0.0	10.334029227557412	0.0
23	6.05125412241687E-4	0.0	0.0	10.63538168285377	0.0
24	6.05125412241687E-4	0.0	0.0	10.845965326313879	0.0
25	6.05125412241687E-4	0.0	0.0	11.002692808084475	0.0
26	6.05125412241687E-4	0.0	0.0	11.149738283259206	0.0
27	6.05125412241687E-4	0.0	0.0	11.298901697376781	0.0
28	6.05125412241687E-4	0.0	0.0	11.439290793016852	0.0
29	6.05125412241687E-4	0.0	0.0	11.575746573477353	0.0
30	6.05125412241687E-4	0.0	0.0	11.7361048077214	0.0
31	6.05125412241687E-4	0.0	0.0	11.885268221838976	0.0
32	6.05125412241687E-4	0.0	0.0	12.050164896674836	0.0
33	6.05125412241687E-4	0.0	0.0	12.190856555021028	0.0
34	6.05125412241687E-4	0.0	0.0	12.327009772775408	0.0
35	6.05125412241687E-4	0.0	0.0	12.49644488820308	0.0
36	6.05125412241687E-4	0.0	0.0	12.657408247859369	0.0
37	6.05125412241687E-4	0.0	0.0	12.821699797282987	0.0
38	6.05125412241687E-4	0.0	0.0	12.988714411061693	0.0
39	6.05125412241687E-4	0.0	0.0	13.14967777071798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCAC	25	3.884756E-5	45.0	8
TACGGGT	20	7.0259866E-4	45.0	4
CGCGACC	40	6.7902874E-9	45.0	10
ATACGGG	25	3.884756E-5	45.0	25
ACGGTCC	20	7.0259866E-4	45.0	10
CGTTTTT	6520	0.0	44.13727	1
GGCACCG	90	0.0	42.5	8
GCACCGA	90	0.0	42.5	9
ACACGCG	70	0.0	41.785717	36
CGGGAAC	65	0.0	41.53846	6
TTACGGG	65	0.0	41.53846	3
CTTTGCG	125	0.0	41.4	1
TGTTTCG	55	6.002665E-11	40.909092	1
CGGGTAC	55	6.002665E-11	40.909092	6
ATTGCGG	50	1.0768417E-9	40.5	2
GCGCGAC	45	1.9212166E-8	40.0	9
ACCGATG	40	3.4482582E-7	39.375	11
TGTTCGG	120	0.0	39.374996	2
GTTTTTT	7675	0.0	38.990227	2
TGGGCGA	445	0.0	38.932583	6
>>END_MODULE